2cm1 - Revision history

OCA at 14:21, 13 December 2023

2023-12-13T14:21:02Z

←Older revision		Revision as of 14:21, 13 December 2023
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	<StructureSection load='2cm1' size='340' side='right'caption='[[2cm1]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2cm1' size='340' side='right'caption='[[2cm1]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2cm1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CM1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2cm1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CM1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1txo\|1txo]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm1 OCA], [https://pdbe.org/2cm1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cm1 RCSB], [https://www.ebi.ac.uk/pdbsum/2cm1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cm1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm1 OCA], [https://pdbe.org/2cm1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cm1 RCSB], [https://www.ebi.ac.uk/pdbsum/2cm1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cm1 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PSTP_MYCTU PSTP_MYCTU] The only predicted protein phosphatase in M.tuberculosis, it dephosphorylates at least 5 protein kinases (PknA, PknB, PknD, PknE and PknF) and the penicillin-binding protein PBPA.<ref>PMID:14575702</ref> <ref>PMID:12950916</ref> <ref>PMID:15967413</ref> <ref>PMID:16436437</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu]]~~	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	~~[[Category: Phosphoprotein phosphatase~~]]	+	[[Category: Alzari PM]]
-	[[Category: Alzari~~, P M~~]]	+	[[Category: Bellinzoni M]]
-	[[Category: Bellinzoni, M]]	+	[[Category: Villarino A]]
-	[[Category: Villarino, A]]	+	[[Category: Wehenkel A]]
-	[[Category: Wehenkel, A]]	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase hypothetical protein]]~~	+
-	~~[[Category: Mycobacterium tuberculosis]]~~	+
-	~~[[Category: Ser/thr protein phosphatase pstp~~]]	+

OCA at 11:31, 30 March 2022

2022-03-30T11:31:51Z

←Older revision		Revision as of 11:31, 30 March 2022
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	<StructureSection load='2cm1' size='340' side='right'caption='[[2cm1]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2cm1' size='340' side='right'caption='[[2cm1]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2cm1]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2CM1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2cm1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CM1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1txo\|1txo]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1txo\|1txo]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2cm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm1 OCA], [~~http~~://pdbe.org/2cm1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2cm1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2cm1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2cm1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm1 OCA], [https://pdbe.org/2cm1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cm1 RCSB], [https://www.ebi.ac.uk/pdbsum/2cm1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cm1 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:38, 25 June 2020

2020-06-25T07:38:55Z

←Older revision		Revision as of 07:38, 25 June 2020
Line 1:		Line 1:

	==Crystal structure of the catalytic domain of serine threonine protein phosphatase PstP in complex with 2 Manganese ions.==		==Crystal structure of the catalytic domain of serine threonine protein phosphatase PstP in complex with 2 Manganese ions.==
-	<StructureSection load='2cm1' size='340' side='right' caption='[[2cm1]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='2cm1' size='340' side='right'caption='[[2cm1]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2cm1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2CM1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2cm1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2CM1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1txo\|1txo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1txo\|1txo]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2cm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm1 OCA], [http://pdbe.org/2cm1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2cm1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cm1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2cm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm1 OCA], [http://pdbe.org/2cm1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2cm1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cm1 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[~~Serine/threonine protein~~ phosphatase\|~~Serine/threonine protein~~ phosphatase]]	+	*[[Protein phosphatase 3D structures\|Protein phosphatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Myctu]]		[[Category: Myctu]]
	[[Category: Phosphoprotein phosphatase]]		[[Category: Phosphoprotein phosphatase]]

OCA at 08:38, 23 May 2018

2018-05-23T08:38:06Z

←Older revision		Revision as of 08:38, 23 May 2018
Line 1:		Line 1:
-	==~~CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF SERINE THREONINE PROTEIN PHOSPHATASE PSTP IN COMPLEX WITH~~ 2 ~~MANGANESE IONS~~.==	+
		+	==Crystal structure of the catalytic domain of serine threonine protein phosphatase PstP in complex with 2 Manganese ions.==
	<StructureSection load='2cm1' size='340' side='right' caption='[[2cm1]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2cm1' size='340' side='right' caption='[[2cm1]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1txo\|1txo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1txo\|1txo]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm1 OCA], [http://pdbe.org/2cm1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2cm1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm1 OCA], [http://pdbe.org/2cm1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2cm1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cm1 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cm/2cm1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cm/2cm1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 06:43, 9 February 2016

2016-02-09T06:43:07Z

←Older revision		Revision as of 06:43, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cm1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 19:37, 10 September 2015

2015-09-10T19:37:17Z

←Older revision		Revision as of 19:37, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2cm1' size='340' side='right' caption='[[2cm1]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2cm1' size='340' side='right' caption='[[2cm1]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2cm1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis Mycobacterium tuberculosis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CM1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2cm1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CM1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1txo\|1txo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1txo\|1txo]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2cm1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm1 OCA], [http://pdbe.org/2cm1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2cm1 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2cm1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[Protein phosphatase\|Protein phosphatase]]
	*[[Serine/threonine protein phosphatase\|Serine/threonine protein phosphatase]]		*[[Serine/threonine protein phosphatase\|Serine/threonine protein phosphatase]]
	== References ==		== References ==
Line 34:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mycobacterium tuberculosis~~]]	+	[[Category: Myctu]]
	[[Category: Phosphoprotein phosphatase]]		[[Category: Phosphoprotein phosphatase]]
	[[Category: Alzari, P M]]		[[Category: Alzari, P M]]
Line 42:		Line 42:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Hydrolase hypothetical protein]]		[[Category: Hydrolase hypothetical protein]]
		+	[[Category: Mycobacterium tuberculosis]]
	[[Category: Ser/thr protein phosphatase pstp]]		[[Category: Ser/thr protein phosphatase pstp]]

OCA at 17:03, 15 January 2015

2015-01-15T17:03:33Z

←Older revision		Revision as of 17:03, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cm1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CM1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cm1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CM1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1txo\|1txo]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1txo\|1txo]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2cm1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2cm1 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Phosphoprotein phosphatase]]		[[Category: Phosphoprotein phosphatase]]
-	[[Category: Alzari, P M.]]	+	[[Category: Alzari, P M]]
-	[[Category: Bellinzoni, M.]]	+	[[Category: Bellinzoni, M]]
-	[[Category: Villarino, A.]]	+	[[Category: Villarino, A]]
-	[[Category: Wehenkel, A.]]	+	[[Category: Wehenkel, A]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Hydrolase hypothetical protein]]		[[Category: Hydrolase hypothetical protein]]
-	[[Category: Mycobacterium tuberculosis]]
	[[Category: Ser/thr protein phosphatase pstp]]		[[Category: Ser/thr protein phosphatase pstp]]

OCA at 00:27, 30 September 2014

2014-09-30T00:27:31Z

←Older revision		Revision as of 00:27, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2cm1.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF SERINE THREONINE PROTEIN PHOSPHATASE PSTP IN COMPLEX WITH 2 MANGANESE IONS.==
		+	<StructureSection load='2cm1' size='340' side='right' caption='[[2cm1]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2cm1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CM1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1txo\|1txo]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cm1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2cm1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cm/2cm1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phospho-Ser/Thr protein phosphatases (PPs) are dinuclear metalloenzymes classed into two large families, PPP and PPM, on the basis of sequence similarity and metal ion dependence. The archetype of the PPM family is the alpha isoform of human PP2C (PP2Calpha), which folds into an alpha/beta domain similar to those of PPP enzymes. The recent structural studies of three bacterial PPM phosphatases, Mycobacterium tuberculosis MtPstP, Mycobacterium smegmatis MspP, and Streptococcus agalactiae STP, confirmed the conservation of the overall fold and dinuclear metal center in the family, but surprisingly revealed the presence of a third conserved metal-binding site in the active site. To gain insight into the roles of the three-metal center in bacterial enzymes, we report structural and metal-binding studies of MtPstP and MspP. The structure of MtPstP in a new trigonal crystal form revealed a fully active enzyme with the canonical dinuclear metal center but without the third metal ion bound to the catalytic site. The absence of metal correlates with a partially unstructured flap segment, indicating that the third manganese ion contributes to reposition the flap, but is dispensable for catalysis. Studies of metal binding to MspP using isothermal titration calorimetry revealed that the three Mn(2+)-binding sites display distinct affinities, with dissociation constants in the nano- and micromolar range for the two catalytic metal ions and a significantly lower affinity for the third metal-binding site. In agreement, the structure of inactive MspP at acidic pH was determined at atomic resolution and shown to lack the third metal ion in the active site. Structural comparisons of all bacterial phosphatases revealed positional variations in the third metal-binding site that are correlated with the presence of bound substrate and the conformation of the flap segment, supporting a role of this metal ion in assisting enzyme-substrate interactions.

-	~~{{STRUCTURE_2cm1\| PDB=2cm1 \| SCENE= }}~~	+	Structural and binding studies of the three-metal center in two mycobacterial PPM Ser/Thr protein phosphatases.,Wehenkel A, Bellinzoni M, Schaeffer F, Villarino A, Alzari PM J Mol Biol. 2007 Dec 7;374(4):890-8. Epub 2007 Oct 2. PMID:17961594<ref>PMID:17961594</ref>

-	~~===CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF SERINE THREONINE PROTEIN PHOSPHATASE PSTP IN COMPLEX WITH 2 MANGANESE IONS~~.~~===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_17961594}}~~	+	==See Also==
-		+	*[[Protein phosphatase\|Protein phosphatase]]
-	==~~About this Structure~~==	+	*[[Serine/threonine protein phosphatase\|Serine/threonine protein phosphatase]]
-	[[~~2cm1~~]] ~~is a 1 chain structure with sequence from~~ [~~http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from~~ [~~http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA~~].	+	== References ==
-		+	<references/>
-	==~~Reference~~==	+	__TOC__
-	<~~ref group="xtra">PMID:017961594<~~/~~ref~~><~~references group="xtra"~~/>	+	</StructureSection>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Phosphoprotein phosphatase]]		[[Category: Phosphoprotein phosphatase]]

OCA: Protected "2cm1" [edit=sysop:move=sysop]

2013-01-06T09:50:37Z

Protected "2cm1" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:50, 6 January 2013

OCA at 09:50, 6 January 2013

2013-01-06T09:50:34Z

←Older revision		Revision as of 09:50, 6 January 2013
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-	{{Seed}}
	[[Image:2cm1.png\|left\|200px]]		[[Image:2cm1.png\|left\|200px]]

-	<!--
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-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_2cm1\| PDB=2cm1 \| SCENE= }}		{{STRUCTURE_2cm1\| PDB=2cm1 \| SCENE= }}

	===CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF SERINE THREONINE PROTEIN PHOSPHATASE PSTP IN COMPLEX WITH 2 MANGANESE IONS.===		===CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF SERINE THREONINE PROTEIN PHOSPHATASE PSTP IN COMPLEX WITH 2 MANGANESE IONS.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17961594}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17961594 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17961594}}		{{ABSTRACT_PUBMED_17961594}}

	==About this Structure==		==About this Structure==
-	~~2CM1~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA].	+	[[2cm1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CM1 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17961594~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017961594</ref><references group="xtra"/>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Phosphoprotein phosphatase]]		[[Category: Phosphoprotein phosphatase]]
Line 30:		Line 18:
	[[Category: Villarino, A.]]		[[Category: Villarino, A.]]
	[[Category: Wehenkel, A.]]		[[Category: Wehenkel, A.]]
		+	[[Category: Hydrolase]]
	[[Category: Hydrolase hypothetical protein]]		[[Category: Hydrolase hypothetical protein]]
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Ser/thr protein phosphatase pstp]]		[[Category: Ser/thr protein phosphatase pstp]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 07:36:57 2009''