2d1u - Revision history

OCA at 06:39, 1 May 2024

2024-05-01T06:39:29Z

←Older revision		Revision as of 06:39, 1 May 2024
Line 1:		Line 1:

	==Solution structure of the periplasmic signaling domain of FecA from Escherichia coli==		==Solution structure of the periplasmic signaling domain of FecA from Escherichia coli==
-	<StructureSection load='2d1u' size='340' side='right'caption='[[2d1u~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2d1u' size='340' side='right'caption='[[2d1u]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2d1u]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2D1U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2d1u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D1U FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>fecA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2d1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d1u OCA], [~~http~~://pdbe.org/2d1u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2d1u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2d1u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2d1u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d1u OCA], [https://pdbe.org/2d1u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d1u RCSB], [https://www.ebi.ac.uk/pdbsum/2d1u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d1u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/FECA_ECOLI FECA_ECOLI]] FecA is the outer membrane receptor protein in the Fe(3+) dicitrate transport system.	+	[https://www.uniprot.org/uniprot/FECA_ECOLI FECA_ECOLI] FecA is the outer membrane receptor protein in the Fe(3+) dicitrate transport system.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d1u ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d1u ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Gram-negative bacteria possess outer membrane receptors that utilize energy provided by the TonB system to take up iron. Several of these receptors participate in extracytoplasmic factor (ECF) signalling through an N-terminal signalling domain that interacts with a periplasmic transmembrane anti-sigma factor protein and a cytoplasmic sigma factor protein. The structures of the intact TonB-dependent outer membrane receptor FecA from Escherichia coli and FpvA from Pseudomonas aeruginosa have recently been solved by protein crystallography; however, no electron density was detected for their periplasmic signalling domains, suggesting that it was either unfolded or flexible with respect to the remainder of the protein. Here we describe the well-defined solution structure of this domain solved by multidimensional nuclear magnetic resonance (NMR) spectroscopy. The monomeric protein construct contains the 79-residue N-terminal domain as well as the next 17 residues that are part of the receptor's plug domain. These form two clearly distinct regions: a highly structured domain at the N-terminal end followed by an extended flexible tail at the C-terminal end, which includes the 'TonB-box' region, and connects it to the plug domain of the receptor. The structured region consists of two alpha-helices that are positioned side by side and are sandwiched in between two small beta-sheets. This is a novel protein fold which appears to be preserved in all the periplasmic signalling domains of bacterial TonB-dependent outer membrane receptors that are involved in ECF signalling, because the hydrophobic residues that make up the core of the protein domain are highly conserved.
-
-	Nuclear magnetic resonance solution structure of the periplasmic signalling domain of the TonB-dependent outer membrane transporter FecA from Escherichia coli.,Garcia-Herrero A, Vogel HJ Mol Microbiol. 2005 Dec;58(5):1226-37. PMID:16313612<ref>PMID:16313612</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2d1u" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Garcia-Herrero, A]]	+	[[Category: Garcia-Herrero A]]
-	[[Category: Vogel~~, H J]]~~	+	[[Category: Vogel HJ]]
-	~~[[Category: Feca]]~~	+
-	~~[[Category: Iron-uptake]]~~	+
-	~~[[Category: Metal transport]]~~	+
-	~~[[Category: Surface signaling~~]]	+

OCA at 09:40, 9 September 2020

2020-09-09T09:40:12Z

←Older revision		Revision as of 09:40, 9 September 2020
Line 1:		Line 1:

-	==Solution ~~strcuture~~ of the periplasmic signaling domain of FecA from Escherichia coli==	+	==Solution structure of the periplasmic signaling domain of FecA from Escherichia coli==
-	<StructureSection load='2d1u' size='340' side='right' caption='[[2d1u]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2d1u' size='340' side='right'caption='[[2d1u]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2d1u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2D1U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2d1u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2D1U FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fecA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fecA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2d1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d1u OCA], [http://pdbe.org/2d1u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d1u RCSB], [http://www.ebi.ac.uk/pdbsum/2d1u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2d1u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2d1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d1u OCA], [http://pdbe.org/2d1u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d1u RCSB], [http://www.ebi.ac.uk/pdbsum/2d1u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2d1u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Garcia-Herrero, A]]		[[Category: Garcia-Herrero, A]]
	[[Category: Vogel, H J]]		[[Category: Vogel, H J]]

OCA at 06:56, 30 May 2018

2018-05-30T06:56:57Z

←Older revision		Revision as of 06:56, 30 May 2018
Line 1:		Line 1:
		+
	==Solution strcuture of the periplasmic signaling domain of FecA from Escherichia coli==		==Solution strcuture of the periplasmic signaling domain of FecA from Escherichia coli==
	<StructureSection load='2d1u' size='340' side='right' caption='[[2d1u]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2d1u' size='340' side='right' caption='[[2d1u]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2d1u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D1U FirstGlance]. <br>		<table><tr><td colspan='2'>[[2d1u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D1U FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fecA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fecA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d1u OCA], [http://pdbe.org/2d1u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d1u RCSB], [http://www.ebi.ac.uk/pdbsum/2d1u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d1u OCA], [http://pdbe.org/2d1u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d1u RCSB], [http://www.ebi.ac.uk/pdbsum/2d1u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2d1u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d1/2d1u_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d1/2d1u_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d1u ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:43, 11 September 2015

2015-09-11T22:43:52Z

←Older revision		Revision as of 22:43, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2d1u' size='340' side='right' caption='[[2d1u]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2d1u' size='340' side='right' caption='[[2d1u]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2d1u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D1U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2d1u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D1U FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fecA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fecA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d1u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d1u RCSB], [http://www.ebi.ac.uk/pdbsum/2d1u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d1u OCA], [http://pdbe.org/2d1u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d1u RCSB], [http://www.ebi.ac.uk/pdbsum/2d1u PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2d1u" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Garcia-Herrero, A]]		[[Category: Garcia-Herrero, A]]
	[[Category: Vogel, H J]]		[[Category: Vogel, H J]]

OCA at 20:25, 25 December 2014

2014-12-25T20:25:57Z

←Older revision		Revision as of 20:25, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2d1u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D1U FirstGlance]. <br>		<table><tr><td colspan='2'>[[2d1u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D1U FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fecA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fecA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d1u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d1u RCSB], [http://www.ebi.ac.uk/pdbsum/2d1u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d1u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d1u RCSB], [http://www.ebi.ac.uk/pdbsum/2d1u PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FECA_ECOLI FECA_ECOLI]] FecA is the outer membrane receptor protein in the Fe(3+) dicitrate transport system.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Garcia-Herrero, A.]]	+	[[Category: Garcia-Herrero, A]]
-	[[Category: Vogel, H J.]]	+	[[Category: Vogel, H J]]
	[[Category: Feca]]		[[Category: Feca]]
	[[Category: Iron-uptake]]		[[Category: Iron-uptake]]
	[[Category: Metal transport]]		[[Category: Metal transport]]
	[[Category: Surface signaling]]		[[Category: Surface signaling]]

OCA at 02:17, 30 September 2014

2014-09-30T02:17:47Z

←Older revision		Revision as of 02:17, 30 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2d1u.~~png\|left~~\|200px]]	+	==Solution strcuture of the periplasmic signaling domain of FecA from Escherichia coli==
		+	<StructureSection load='2d1u' size='340' side='right' caption='[[2d1u]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2d1u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D1U FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fecA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d1u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d1u RCSB], [http://www.ebi.ac.uk/pdbsum/2d1u PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d1/2d1u_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Gram-negative bacteria possess outer membrane receptors that utilize energy provided by the TonB system to take up iron. Several of these receptors participate in extracytoplasmic factor (ECF) signalling through an N-terminal signalling domain that interacts with a periplasmic transmembrane anti-sigma factor protein and a cytoplasmic sigma factor protein. The structures of the intact TonB-dependent outer membrane receptor FecA from Escherichia coli and FpvA from Pseudomonas aeruginosa have recently been solved by protein crystallography; however, no electron density was detected for their periplasmic signalling domains, suggesting that it was either unfolded or flexible with respect to the remainder of the protein. Here we describe the well-defined solution structure of this domain solved by multidimensional nuclear magnetic resonance (NMR) spectroscopy. The monomeric protein construct contains the 79-residue N-terminal domain as well as the next 17 residues that are part of the receptor's plug domain. These form two clearly distinct regions: a highly structured domain at the N-terminal end followed by an extended flexible tail at the C-terminal end, which includes the 'TonB-box' region, and connects it to the plug domain of the receptor. The structured region consists of two alpha-helices that are positioned side by side and are sandwiched in between two small beta-sheets. This is a novel protein fold which appears to be preserved in all the periplasmic signalling domains of bacterial TonB-dependent outer membrane receptors that are involved in ECF signalling, because the hydrophobic residues that make up the core of the protein domain are highly conserved.

-	~~{{STRUCTURE_2d1u\| PDB=2d1u \| SCENE= }}~~	+	Nuclear magnetic resonance solution structure of the periplasmic signalling domain of the TonB-dependent outer membrane transporter FecA from Escherichia coli.,Garcia-Herrero A, Vogel HJ Mol Microbiol. 2005 Dec;58(5):1226-37. PMID:16313612<ref>PMID:16313612</ref>

-	~~===Solution strcuture~~ of the ~~periplasmic signaling domain of FecA from Escherichia coli===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16313612}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2d1u]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016313612<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Garcia-Herrero, A.]]		[[Category: Garcia-Herrero, A.]]

OCA: Protected "2d1u" [edit=sysop:move=sysop]

2013-01-06T11:30:29Z

Protected "2d1u" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:30, 6 January 2013

OCA at 11:30, 6 January 2013

2013-01-06T11:30:25Z

←Older revision		Revision as of 11:30, 6 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2d1u.png\|left\|200px]]		[[Image:2d1u.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2d1u", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2d1u\| PDB=2d1u \| SCENE= }}		{{STRUCTURE_2d1u\| PDB=2d1u \| SCENE= }}

	===Solution strcuture of the periplasmic signaling domain of FecA from Escherichia coli===		===Solution strcuture of the periplasmic signaling domain of FecA from Escherichia coli===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16313612}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16313612 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16313612}}		{{ABSTRACT_PUBMED_16313612}}

	==About this Structure==		==About this Structure==
-	~~2D1U~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA].	+	[[2d1u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16313612~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016313612</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Garcia-Herrero, A.]]		[[Category: Garcia-Herrero, A.]]
Line 29:		Line 17:
	[[Category: Feca]]		[[Category: Feca]]
	[[Category: Iron-uptake]]		[[Category: Iron-uptake]]
		+	[[Category: Metal transport]]
	[[Category: Surface signaling]]		[[Category: Surface signaling]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:11:23 2009''

OCA at 04:11, 18 February 2009

2009-02-18T04:11:23Z

←Older revision		Revision as of 04:11, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2D1U is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA].	+	2D1U is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA].

	==Reference==		==Reference==
-	Nuclear magnetic resonance solution structure of the periplasmic signalling domain of the TonB-dependent outer membrane transporter FecA from Escherichia coli., Garcia-Herrero A, Vogel HJ, Mol Microbiol. 2005 Dec;58(5):1226-37. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16313612 16313612]~~	+	<ref group="xtra">PMID:16313612</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Garcia-Herrero, A.]]		[[Category: Garcia-Herrero, A.]]
	[[Category: Vogel, H J.]]		[[Category: Vogel, H J.]]
Line 32:		Line 31:
	[[Category: Surface signaling]]		[[Category: Surface signaling]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 12~~:54:~~02 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:11:23 2009''

OCA at 09:54, 29 July 2008

2008-07-29T09:54:04Z

←Older revision		Revision as of 09:54, 29 July 2008
Line 1:		Line 1:
-	[[Image:2d1u.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2d1u.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2d1u\| PDB=2d1u \| SCENE= }}		{{STRUCTURE_2d1u\| PDB=2d1u \| SCENE= }}

-	~~'''~~Solution strcuture of the periplasmic signaling domain of FecA from Escherichia coli~~'''~~	+	===Solution strcuture of the periplasmic signaling domain of FecA from Escherichia coli===


-	~~==Overview==~~	+	<!--
-	~~Gram~~-negative bacteria possess outer membrane receptors that utilize energy provided by the TonB system to take up iron. Several of these receptors participate in extracytoplasmic factor (ECF) signalling through an N-~~terminal signalling domain that interacts with a periplasmic transmembrane anti-sigma factor protein and a cytoplasmic sigma factor protein.~~ The ~~structures of the intact TonB-dependent outer membrane receptor FecA from Escherichia coli and FpvA from Pseudomonas aeruginosa have recently been solved by protein crystallography; however~~, ~~no electron density was detected for their periplasmic signalling domains~~, ~~suggesting that it was either unfolded or flexible with respect~~ to the ~~remainder of the protein. Here we describe the well-defined solution structure of this domain solved by multidimensional nuclear magnetic resonance~~ (~~NMR) spectroscopy. The monomeric protein construct contains the 79-residue N-terminal domain~~ as ~~well as the next 17 residues that are part of the receptor's plug domain~~. ~~These form two clearly distinct regions: a highly structured domain at the N-terminal end followed by an extended flexible tail at the C-terminal end~~, ~~which includes~~ the ~~'TonB-box' region, and connects it to the plug domain of the receptor~~. ~~The structured region consists of two alpha~~-~~helices that are positioned side by side and are sandwiched in between two small beta~~-sheets. This is a novel protein fold which appears to be preserved in all the periplasmic signalling domains of bacterial TonB-dependent outer membrane receptors that are involved in ECF signalling, because the hydrophobic residues that make up the core of the protein domain are highly conserved.	+	The line below this paragraph, {{ABSTRACT_PUBMED_16313612}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 16313612 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_16313612}}

	==About this Structure==		==About this Structure==
-	2D1U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA].	+	2D1U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA].

	==Reference==		==Reference==
Line 27:		Line 31:
	[[Category: Iron-uptake]]		[[Category: Iron-uptake]]
	[[Category: Surface signaling]]		[[Category: Surface signaling]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 23~~:32:08 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:54:02 2008''