2drg - Revision history

OCA at 10:35, 8 December 2021

2021-12-08T10:35:03Z

←Older revision		Revision as of 10:35, 8 December 2021
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	<StructureSection load='2drg' size='340' side='right'caption='[[2drg]]' scene=''>		<StructureSection load='2drg' size='340' side='right'caption='[[2drg]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2DRG FirstGlance]. <br>	+	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DRG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2drg FirstGlance], [~~http~~://www.ebi.ac.uk/pdbsum/2drg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2drg ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2drg FirstGlance], [https://www.ebi.ac.uk/pdbsum/2drg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2drg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:57, 4 March 2020

2020-03-04T07:57:05Z

←Older revision		Revision as of 07:57, 4 March 2020
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	==1-O-SINAPOYL-BETA-GLUCOSE:CHOLINE SINAPOYLTRANSFERASE==		==1-O-SINAPOYL-BETA-GLUCOSE:CHOLINE SINAPOYLTRANSFERASE==
-	<StructureSection load='2drg' size='340' side='right' caption='[[2drg]]' scene=''>	+	<StructureSection load='2drg' size='340' side='right'caption='[[2drg]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRG FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRG FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Theoretical Model]]		[[Category: Theoretical Model]]
		+	[[Category: Large Structures]]
	[[Category: Brandt, W]]		[[Category: Brandt, W]]
	[[Category: Stehle, F]]		[[Category: Stehle, F]]

OCA at 06:50, 30 May 2018

2018-05-30T06:50:19Z

←Older revision		Revision as of 06:50, 30 May 2018
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRG FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2drg FirstGlance], [http://www.ebi.ac.uk/pdbsum/2drg PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2drg FirstGlance], [http://www.ebi.ac.uk/pdbsum/2drg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2drg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:31, 11 February 2016

2016-02-11T10:31:47Z

←Older revision		Revision as of 10:31, 11 February 2016
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		+	{{Theoretical_model}}
		+
	==1-O-SINAPOYL-BETA-GLUCOSE:CHOLINE SINAPOYLTRANSFERASE==		==1-O-SINAPOYL-BETA-GLUCOSE:CHOLINE SINAPOYLTRANSFERASE==
	<StructureSection load='2drg' size='340' side='right' caption='[[2drg]]' scene=''>		<StructureSection load='2drg' size='340' side='right' caption='[[2drg]]' scene=''>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Theoretical Model]]
	[[Category: Brandt, W]]		[[Category: Brandt, W]]
	[[Category: Stehle, F]]		[[Category: Stehle, F]]

OCA at 06:39, 10 September 2015

2015-09-10T06:39:35Z

←Older revision		Revision as of 06:39, 10 September 2015
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2drg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:33, 15 January 2015

2015-01-15T18:33:31Z

←Older revision		Revision as of 18:33, 15 January 2015
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRG FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2drg FirstGlance], [http://www.ebi.ac.uk/pdbsum/2drg PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2drg FirstGlance], [http://www.ebi.ac.uk/pdbsum/2drg PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 04:17, 29 September 2014

2014-09-29T04:17:46Z

←Older revision		Revision as of 04:17, 29 September 2014
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-	~~{{Theoretical_model}}~~	+	==1-O-SINAPOYL-BETA-GLUCOSE:CHOLINE SINAPOYLTRANSFERASE==
		+	<StructureSection load='2drg' size='340' side='right' caption='[[2drg]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DRG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2drg FirstGlance], [http://www.ebi.ac.uk/pdbsum/2drg PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Structures of the serine carboxypeptidase-like enzymes 1-O-sinapoyl-beta-glucose:L-malate sinapoyltransferase (SMT) and 1-O-sinapoyl-beta-glucose:choline sinapoyltransferase (SCT) were modeled to gain insight into determinants of specificity and substrate recognition. The structures reveal the alpha/beta-hydrolase fold as scaffold for the catalytic triad Ser-His-Asp. The recombinant mutants of SMT Ser173Ala and His411Ala were inactive, whereas Asp358Ala displayed residual activity of 20%. 1-O-sinapoyl-beta-glucose recognition is mediated by a network of hydrogen bonds. The glucose moiety is recognized by a hydrogen bond network including Trp71, Asn73, Glu87 and Asp172. The conserved Asp172 at the sequence position preceding the catalytic serine meets sterical requirements for the glucose moiety. The mutant Asn73Ala with a residual activity of 13% underscores the importance of the intact hydrogen bond network. Arg322 is of key importance by hydrogen bonding of 1-O-sinapoyl-beta-glucose and L-malate. By conformational change, Arg322 transfers L-malate to a position favoring its activation by His411. Accordingly, the mutant Arg322Glu showed 1% residual activity. Glu215 and Arg219 establish hydrogen bonds with the sinapoyl moiety. The backbone amide hydrogens of Gly75 and Tyr174 were shown to form the oxyanion hole, stabilizing the transition state. SCT reveals also the catalytic triad and a hydrogen bond network for 1-O-sinapoyl-beta-glucose recognition, but Glu274, Glu447, Thr445 and Cys281 are crucial for positioning of choline.

-	~~[[Image~~:~~2drg~~.~~png\|left\|200px]]~~	+	Structure determinants and substrate recognition of serine carboxypeptidase-like acyltransferases from plant secondary metabolism.,Stehle F, Brandt W, Milkowski C, Strack D FEBS Lett. 2006 Nov 27;580(27):6366-74. Epub 2006 Nov 10. PMID:17094968<ref>PMID:17094968</ref>

-	~~{{STRUCTURE_2drg\| PDB=2drg \| SCENE= }}~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	==~~=1-O-SINAPOYL-BETA-GLUCOSE:CHOLINE SINAPOYLTRANSFERASE===~~	+	== References ==
-		+	<references/>
-	~~{{ABSTRACT_PUBMED_17094968}}~~	+	__TOC__
-		+	</StructureSection>
-	~~==Reference~~==	+
-	<~~ref group="xtra">PMID:017094968<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Brandt, W]]		[[Category: Brandt, W]]
	[[Category: Stehle, F]]		[[Category: Stehle, F]]

OCA: Protected "2drg" [edit=sysop:move=sysop]

2012-10-21T10:01:57Z

Protected "2drg" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:01, 21 October 2012

OCA at 10:01, 21 October 2012

2012-10-21T10:01:54Z

←Older revision		Revision as of 10:01, 21 October 2012
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	{{Theoretical_model}}		{{Theoretical_model}}
-	~~{{Seed}}~~	+
	[[Image:2drg.png\|left\|200px]]		[[Image:2drg.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2drg\| PDB=2drg \| SCENE= }}		{{STRUCTURE_2drg\| PDB=2drg \| SCENE= }}

	===1-O-SINAPOYL-BETA-GLUCOSE:CHOLINE SINAPOYLTRANSFERASE===		===1-O-SINAPOYL-BETA-GLUCOSE:CHOLINE SINAPOYLTRANSFERASE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17094968}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17094968 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17094968}}		{{ABSTRACT_PUBMED_17094968}}
-
-	==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DRG OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17094968~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017094968</ref><references group="xtra"/>
	[[Category: Brandt, W]]		[[Category: Brandt, W]]
	[[Category: Stehle, F]]		[[Category: Stehle, F]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 09:25:34 2010''

OCA at 06:25, 8 April 2010

2010-04-08T06:25:35Z

←Older revision		Revision as of 06:25, 8 April 2010
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-	~~'''Theoretical Model'''~~	+	{{Theoretical_model}}
		+	{{Seed}}
		+	[[Image:2drg.png\|left\|200px]]

-	The ~~entry 2DRG~~ is ~~a Theoretical Model titled '1~~-O-~~sinapoyl-beta-glucose:choline sinapoyltransferase'.~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2drg", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2drg\| PDB=2drg \| SCENE= }}

-	~~[[Category~~:~~Theoretical Model]]~~	+	===1-O-SINAPOYL-BETA-GLUCOSE:CHOLINE SINAPOYLTRANSFERASE===


-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 30~~ 09:56:~~18 2008~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_17094968}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17094968 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_17094968}}
		+
		+	==About this Structure==
		+	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DRG OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:17094968</ref><references group="xtra"/>
		+	[[Category: Brandt, W]]
		+	[[Category: Stehle, F]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 09:25:34 2010''