2ehn - Revision history

OCA: Redirecting to 3ah4

2010-04-28T07:39:25Z

Redirecting to 3ah4

←Older revision		Revision as of 07:39, 28 April 2010
Line 1:		Line 1:
-	~~{{Seed}}~~	+	#REDIRECT [[3ah4]] This PDB entry is obsolete and replaced by 3ah4
-	[[~~Image:2ehn.png\|left\|200px~~]]	+
-		+
-	~~<!--~~	+
-	~~The line below this paragraph, containing "STRUCTURE_2ehn", creates the "Structure Box" on the page.~~	+
-	~~You may change the~~ PDB ~~parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page~~ is ~~loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	~~-->~~	+
-	~~{{STRUCTURE_2ehn\| PDB=2ehn \| SCENE= }}~~	+
-		+
-	~~===HA1 subcomponent of botulinum type C progenitor toxin complexed with galactose===~~	+
-		+
-		+
-	~~<!--~~	+
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_18178224}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 18178224 is the PubMed ID number.~~	+
-	~~-->~~	+
-	~~{{ABSTRACT_PUBMED_18178224}}~~	+
-		+
-	~~==About this Structure==~~	+
-	2EHN is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EHN OCA].	+
-		+
-	~~==Reference==~~	+
-	~~<ref group="xtra">PMID:18178224</ref><ref group="xtra">PMID:17196748</ref><ref group="xtra">PMID:16413070</ref><references group="xtra"/>~~	+
-	~~[[Category: Clostridium botulinum]]~~	+
-	~~[[Category: Ide, A.]]~~	+
-	~~[[Category: Nakamura, T.]]~~	+
-	~~[[Category: Nishikawa, A.]]~~	+
-	~~[[Category: Oguma, K.]]~~	+
-	~~[[Category: Tonozuka, T.]]~~	+
-	~~[[Category: Yuzawa, T.]]~~	+
-	~~[[Category: Beta trefoil]]~~	+
-	~~[[Category: Toxin]]~~	+
-		+
-	~~''Page seeded~~ by ~~[http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 10:51:45 2009''~~	+

OCA at 08:51, 16 February 2009

2009-02-16T08:51:47Z

←Older revision		Revision as of 08:51, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2EHN is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EHN OCA].	+	2EHN is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EHN OCA].

	==Reference==		==Reference==
-	Sugar-binding sites of the HA1 subcomponent of Clostridium botulinum type C progenitor toxin., Nakamura T, Tonozuka T, Ide A, Yuzawa T, Oguma K, Nishikawa A, J Mol Biol. 2008 Feb 22;376(3):854-67. Epub 2007 Dec 23. PMID:~~[http:~~/~~/www.ncbi.nlm.nih.gov/pubmed/18178224 18178224]~~	+	<ref group="xtra">PMID:18178224</ref><ref group="xtra">PMID:17196748</ref><ref group="xtra">PMID:16413070</ref><references group="xtra"/>
-		+
-	Binding properties of Clostridium botulinum type C progenitor toxin to mucins., Nakamura T, Takada N, Tonozuka T, Sakano Y, Oguma K, Nishikawa A, Biochim Biophys Acta. 2007 Apr;1770(4):551-5. Epub 2006 Nov 23. PMID:~~[http:~~/~~/www.ncbi.nlm.nih.gov/pubmed/17196748 17196748]~~	+
-		+
-	Cell internalization and traffic pathway of Clostridium botulinum type C neurotoxin in HT-29 cells., Uotsu N, Nishikawa A, Watanabe T, Ohyama T, Tonozuka T, Sakano Y, Oguma K, Biochim Biophys Acta. 2006 Jan;1763(1):120-8. Epub 2005 Dec 27. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16413070 16413070]~~	+
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
-	[[Category: Single protein]]
	[[Category: Ide, A.]]		[[Category: Ide, A.]]
	[[Category: Nakamura, T.]]		[[Category: Nakamura, T.]]
Line 39:		Line 34:
	[[Category: Toxin]]		[[Category: Toxin]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 13~~:00:~~57 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 10:51:45 2009''

OCA at 10:01, 27 July 2008

2008-07-27T10:01:00Z

←Older revision		Revision as of 10:01, 27 July 2008
Line 1:		Line 1:
-	[[Image:2ehn.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2ehn.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2ehn\| PDB=2ehn \| SCENE= }}		{{STRUCTURE_2ehn\| PDB=2ehn \| SCENE= }}

-	~~'''~~HA1 subcomponent of botulinum type C progenitor toxin complexed with galactose~~'''~~	+	===HA1 subcomponent of botulinum type C progenitor toxin complexed with galactose===


-	~~==Overview==~~	+	<!--
-	Clostridium botulinum type C 16S progenitor toxin contains a hemagglutinin (HA) subcomponent, designated HA1, which appears to play an important role in the effective internalization of the toxin in gastrointestinal epithelial cells and in creating a broad specificity for the oligosaccharide structure that corresponds to various targets. In this ~~study~~, ~~using the recombinant protein fused to glutathione S-transferase~~, ~~we investigated~~ the ~~binding specificity of the HA1 subcomponent~~ to ~~sugars and estimated~~ the ~~binding sites of HA1 based~~ on X-ray crystallography and soaking experiments using various sugars. N-Acetylneuraminic acid, N-acetylgalactosamine, and galactose effectively inhibited the binding that occurs between glutathione S-transferase-HA1 and mucins, whereas N-acetylglucosamine and glucose did not inhibit it. The crystal structures of HA1 complex with N-acetylneuraminic acid, N-acetylgalactosamine, and galactose were also determined. There are two sugar-binding sites, sites I and II. Site I corresponds to the electron densities noted for all sugars and is located at ~~the C-terminal beta-trefoil domain, while site II corresponds to the electron densities noted only for galactose~~. ~~An aromatic amino acid residue, Trp176, at site I has a stacking interaction with the hexose ring of the sugars~~. ~~On the other hand~~, ~~there~~ is ~~no aromatic residue at site II; thus,~~ the ~~interaction with galactose seems to be poor~~. ~~The double mutant W176A at site I and D271F at site II has no avidity for N~~-~~acetylneuraminic acid but has avidity for galactose. In this report, the binding specificity of botulinum C16S toxin HA1 to various sugars is demonstrated based on its structural features.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_18178224}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18178224 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18178224}}

	==About this Structure==		==About this Structure==
Line 20:		Line 24:
	==Reference==		==Reference==
	Sugar-binding sites of the HA1 subcomponent of Clostridium botulinum type C progenitor toxin., Nakamura T, Tonozuka T, Ide A, Yuzawa T, Oguma K, Nishikawa A, J Mol Biol. 2008 Feb 22;376(3):854-67. Epub 2007 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18178224 18178224]		Sugar-binding sites of the HA1 subcomponent of Clostridium botulinum type C progenitor toxin., Nakamura T, Tonozuka T, Ide A, Yuzawa T, Oguma K, Nishikawa A, J Mol Biol. 2008 Feb 22;376(3):854-67. Epub 2007 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18178224 18178224]
		+
		+	Binding properties of Clostridium botulinum type C progenitor toxin to mucins., Nakamura T, Takada N, Tonozuka T, Sakano Y, Oguma K, Nishikawa A, Biochim Biophys Acta. 2007 Apr;1770(4):551-5. Epub 2006 Nov 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17196748 17196748]
		+
		+	Cell internalization and traffic pathway of Clostridium botulinum type C neurotoxin in HT-29 cells., Uotsu N, Nishikawa A, Watanabe T, Ohyama T, Tonozuka T, Sakano Y, Oguma K, Biochim Biophys Acta. 2006 Jan;1763(1):120-8. Epub 2005 Dec 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16413070 16413070]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
	[[Category: Single protein]]		[[Category: Single protein]]
Line 30:		Line 38:
	[[Category: Beta trefoil]]		[[Category: Beta trefoil]]
	[[Category: Toxin]]		[[Category: Toxin]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun ~~May 4 02~~:34:09 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:00:57 2008''

OCA at 23:34, 3 May 2008

2008-05-03T23:34:10Z

←Older revision		Revision as of 23:34, 3 May 2008
Line 1:		Line 1:
	[[Image:2ehn.jpg\|left\|200px]]		[[Image:2ehn.jpg\|left\|200px]]

-	{{Structure	+	<!--
-	\|PDB~~= 2ehn \|SIZE=350\|CAPTION= <~~scene ~~name='initialview01'>2ehn</scene>~~, ~~resolution 1~~.~~78Å~~	+	The line below this paragraph, containing "STRUCTURE_2ehn", creates the "Structure Box" on the page.
-	~~\|SITE= <scene name='pdbsite=AC1:Gal+Binding+Site+For+Residue+B+2000'>AC1</scene> and <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+B+2001'>AC2</scene>~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <scene name='pdbligand=GAL:BETA~~-D-~~GALACTOSE'>GAL</scene~~>	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	\|~~ACTIVITY~~=	+	or leave the SCENE parameter empty for the default display.
-	\|~~GENE~~=	+	-->
-	~~\|DOMAIN=~~	+	{{STRUCTURE_2ehn\| PDB=2ehn \| SCENE= }}
-	~~\|RELATEDENTRY=[[1qxm\|1QXM]], [[2ehi\|2EHI]], [[2ehm\|2EHM]]~~	+
-	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ehn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ehn OCA], [http://www.ebi.ac.uk/pdbsum/2ehn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ehn RCSB]</span>	+
-	}}	+

	'''HA1 subcomponent of botulinum type C progenitor toxin complexed with galactose'''		'''HA1 subcomponent of botulinum type C progenitor toxin complexed with galactose'''
Line 31:		Line 28:
	[[Category: Tonozuka, T.]]		[[Category: Tonozuka, T.]]
	[[Category: Yuzawa, T.]]		[[Category: Yuzawa, T.]]
-	[[Category: ~~beta~~ trefoil]]	+	[[Category: Beta trefoil]]
-	[[Category: ~~toxin~~]]	+	[[Category: Toxin]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:34:09 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31~~ 02:49:16 2008''	+

OCA at 23:49, 30 March 2008

2008-03-30T23:49:16Z

←Older revision		Revision as of 23:49, 30 March 2008
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	\|ACTIVITY=		\|ACTIVITY=
	\|GENE=		\|GENE=
		+	\|DOMAIN=
		+	\|RELATEDENTRY=[[1qxm\|1QXM]], [[2ehi\|2EHI]], [[2ehm\|2EHM]]
		+	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ehn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ehn OCA], [http://www.ebi.ac.uk/pdbsum/2ehn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ehn RCSB]</span>
	}}		}}

Line 28:		Line 31:
	[[Category: Tonozuka, T.]]		[[Category: Tonozuka, T.]]
	[[Category: Yuzawa, T.]]		[[Category: Yuzawa, T.]]
-	[[Category: GAL]]
	[[Category: beta trefoil]]		[[Category: beta trefoil]]
	[[Category: toxin]]		[[Category: toxin]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Mar ~~20 16~~:40:21 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:49:16 2008''

OCA at 14:40, 20 March 2008

2008-03-20T14:40:25Z

←Older revision		Revision as of 14:40, 20 March 2008
Line 1:		Line 1:
-	[[Image:2ehn.jpg\|left\|200px]]~~<br /><applet load~~="2ehn~~" size~~="350~~" color~~=~~"white" frame="true" align="right" spinBox~~=~~"true"~~	+	[[Image:2ehn.jpg\|left\|200px]]
-	~~caption="~~2ehn, resolution 1.78Å" />	+
-	'''HA1 subcomponent of botulinum type C progenitor toxin complexed with galactose'''~~<br />~~	+	{{Structure
		+	\|PDB= 2ehn \|SIZE=350\|CAPTION= <scene name='initialview01'>2ehn</scene>, resolution 1.78Å
		+	\|SITE= <scene name='pdbsite=AC1:Gal+Binding+Site+For+Residue+B+2000'>AC1</scene> and <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+B+2001'>AC2</scene>
		+	\|LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>
		+	\|ACTIVITY=
		+	\|GENE=
		+	}}
		+
		+	'''HA1 subcomponent of botulinum type C progenitor toxin complexed with galactose'''
		+

	==Overview==		==Overview==
Line 7:		Line 16:

	==About this Structure==		==About this Structure==
-	2EHN is a [~~http://en.wikipedia.org/wiki/Single_protein~~ Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] with <scene name='pdbligand=GAL:'>GAL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Gal+Binding+Site+For+Residue+B+2000'>AC1</scene> and <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+B+2001'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EHN OCA].	+	2EHN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EHN OCA].

	==Reference==		==Reference==
-	Sugar-binding sites of the HA1 subcomponent of Clostridium botulinum type C progenitor toxin., Nakamura T, Tonozuka T, Ide A, Yuzawa T, Oguma K, Nishikawa A, J Mol Biol. 2008 Feb 22;376(3):854-67. Epub 2007 Dec 23. PMID:[http://~~ispc~~.~~weizmann~~.ac.~~il/~~/~~pmbin~~/~~getpm?pmid=~~18178224 18178224]	+	Sugar-binding sites of the HA1 subcomponent of Clostridium botulinum type C progenitor toxin., Nakamura T, Tonozuka T, Ide A, Yuzawa T, Oguma K, Nishikawa A, J Mol Biol. 2008 Feb 22;376(3):854-67. Epub 2007 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18178224 18178224]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
	[[Category: Single protein]]		[[Category: Single protein]]
Line 23:		Line 32:
	[[Category: toxin]]		[[Category: toxin]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu ~~Feb 21 17~~:10:06 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:40:21 2008''

OCA: New page: 200px
'''HA1 subcomponent of ...
2008-02-21T15:10:18Z

New page: 200px<br /><applet load="2ehn" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ehn, resolution 1.78Å" /> '''HA1 subcomponent of ...

New page
[[Image:2ehn.jpg|left|200px]]<br /><applet load="2ehn" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2ehn, resolution 1.78Å" />
'''HA1 subcomponent of botulinum type C progenitor toxin complexed with galactose'''<br />

==Overview==
Clostridium botulinum type C 16S progenitor toxin contains a hemagglutinin (HA) subcomponent, designated HA1, which appears to play an important role in the effective internalization of the toxin in gastrointestinal epithelial cells and in creating a broad specificity for the oligosaccharide structure that corresponds to various targets. In this study, using the recombinant protein fused to glutathione S-transferase, we investigated the binding specificity of the HA1 subcomponent to sugars and estimated the binding sites of HA1 based on X-ray crystallography and soaking experiments using various sugars. N-Acetylneuraminic acid, N-acetylgalactosamine, and galactose effectively inhibited the binding that occurs between glutathione S-transferase-HA1 and mucins, whereas N-acetylglucosamine and glucose did not inhibit it. The crystal structures of HA1 complex with N-acetylneuraminic acid, N-acetylgalactosamine, and galactose were also determined. There are two sugar-binding sites, sites I and II. Site I corresponds to the electron densities noted for all sugars and is located at the C-terminal beta-trefoil domain, while site II corresponds to the electron densities noted only for galactose. An aromatic amino acid residue, Trp176, at site I has a stacking interaction with the hexose ring of the sugars. On the other hand, there is no aromatic residue at site II; thus, the interaction with galactose seems to be poor. The double mutant W176A at site I and D271F at site II has no avidity for N-acetylneuraminic acid but has avidity for galactose. In this report, the binding specificity of botulinum C16S toxin HA1 to various sugars is demonstrated based on its structural features.

==About this Structure==
2EHN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] with <scene name='pdbligand=GAL:'>GAL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Gal+Binding+Site+For+Residue+B+2000'>AC1</scene> and <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+B+2001'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EHN OCA].

==Reference==
Sugar-binding sites of the HA1 subcomponent of Clostridium botulinum type C progenitor toxin., Nakamura T, Tonozuka T, Ide A, Yuzawa T, Oguma K, Nishikawa A, J Mol Biol. 2008 Feb 22;376(3):854-67. Epub 2007 Dec 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18178224 18178224]
[[Category: Clostridium botulinum]]
[[Category: Single protein]]
[[Category: Ide, A.]]
[[Category: Nakamura, T.]]
[[Category: Nishikawa, A.]]
[[Category: Oguma, K.]]
[[Category: Tonozuka, T.]]
[[Category: Yuzawa, T.]]
[[Category: GAL]]
[[Category: beta trefoil]]
[[Category: toxin]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:10:06 2008''