2eic - Revision history

OCA at 08:41, 25 October 2023

2023-10-25T08:41:10Z

←Older revision		Revision as of 08:41, 25 October 2023
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	<StructureSection load='2eic' size='340' side='right'caption='[[2eic]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2eic' size='340' side='right'caption='[[2eic]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2eic]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Fusairum_graminearum Fusairum~~ graminearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EIC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2eic]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_graminearum Fusarium graminearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EIC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>~~, ~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[2eib\|2eib]], [[2eid\|2eid]], [[2eie\|2eie]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>GO ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~5518 Fusairum graminearum])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eic OCA], [https://pdbe.org/2eic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eic RCSB], [https://www.ebi.ac.uk/pdbsum/2eic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eic ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eic OCA], [https://pdbe.org/2eic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eic RCSB], [https://www.ebi.ac.uk/pdbsum/2eic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eic ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GAOA_GIBZA GAOA_GIBZA] Catalyzes the sterospecific oxidation of primary alcohols to the corresponding aldehydes. The biologically relevant substrate of the enzyme is not known as the enzyme exhibits broad substrate specificity from small alcohols through sugars to oligo- and polysaccharides.<ref>PMID:13641238</ref> <ref>PMID:4441089</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Fusairum~~ graminearum]]	+	[[Category: Fusarium graminearum]]
-	~~[[Category: Galactose oxidase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Akyumani, N]]	+	[[Category: Akyumani N]]
-	[[Category: Firbank~~, S J~~]]	+	[[Category: Firbank SJ]]
-	[[Category: Knowles~~, P F~~]]	+	[[Category: Knowles PF]]
-	[[Category: McPherson~~, M J~~]]	+	[[Category: McPherson MJ]]
-	[[Category: Phillips~~, S E~~]]	+	[[Category: Phillips SE]]
-	[[Category: Tamber, S]]	+	[[Category: Tamber S]]
-	~~[[Category: Galactose oxidase w290f mutant]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 06:56, 10 November 2021

2021-11-10T06:56:35Z

←Older revision		Revision as of 06:56, 10 November 2021
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	<StructureSection load='2eic' size='340' side='right'caption='[[2eic]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2eic' size='340' side='right'caption='[[2eic]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2eic]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Fusarium_graminarium Fusarium graminarium~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EIC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2eic]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusairum_graminearum Fusairum graminearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EIC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2eib\|2eib]], [[2eid\|2eid]], [[2eie\|2eie]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2eib\|2eib]], [[2eid\|2eid]], [[2eie\|2eie]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GO ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5518 ~~Fusarium graminarium~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GO ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5518 Fusairum graminearum])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eic OCA], [https://pdbe.org/2eic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eic RCSB], [https://www.ebi.ac.uk/pdbsum/2eic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eic ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eic OCA], [https://pdbe.org/2eic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eic RCSB], [https://www.ebi.ac.uk/pdbsum/2eic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eic ProSAT]</span></td></tr>
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Fusarium graminarium~~]]	+	[[Category: Fusairum graminearum]]
	[[Category: Galactose oxidase]]		[[Category: Galactose oxidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 12:08, 10 February 2021

2021-02-10T12:08:43Z

←Older revision		Revision as of 12:08, 10 February 2021
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	==Crystal Structure of Galactose Oxidase mutant W290F==		==Crystal Structure of Galactose Oxidase mutant W290F==
-	<StructureSection load='2eic' size='340' side='right' caption='[[2eic]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='2eic' size='340' side='right'caption='[[2eic]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2eic]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Fusarium_graminarium Fusarium graminarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2EIC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2eic]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_graminarium Fusarium graminarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EIC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2eib\|2eib]], [[2eid\|2eid]], [[2eie\|2eie]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2eib\|2eib]], [[2eid\|2eid]], [[2eie\|2eie]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GO ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5518 Fusarium graminarium])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GO ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5518 Fusarium graminarium])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eic OCA], [~~http~~://pdbe.org/2eic PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2eic RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2eic PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2eic ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eic OCA], [https://pdbe.org/2eic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eic RCSB], [https://www.ebi.ac.uk/pdbsum/2eic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eic ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	[[Category: Fusarium graminarium]]		[[Category: Fusarium graminarium]]
	[[Category: Galactose oxidase]]		[[Category: Galactose oxidase]]
		+	[[Category: Large Structures]]
	[[Category: Akyumani, N]]		[[Category: Akyumani, N]]
	[[Category: Firbank, S J]]		[[Category: Firbank, S J]]

OCA at 06:57, 6 June 2018

2018-06-06T06:57:31Z

←Older revision		Revision as of 06:57, 6 June 2018
Line 1:		Line 1:
		+
	==Crystal Structure of Galactose Oxidase mutant W290F==		==Crystal Structure of Galactose Oxidase mutant W290F==
	<StructureSection load='2eic' size='340' side='right' caption='[[2eic]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2eic' size='340' side='right' caption='[[2eic]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2eic]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Fusarium_graminearum~~ Fusarium ~~graminearum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2eic]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_graminarium Fusarium graminarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2eib\|2eib]], [[2eid\|2eid]], [[2eie\|2eie]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2eib\|2eib]], [[2eid\|2eid]], [[2eie\|2eie]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5518 Fusarium ~~graminearum~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5518 Fusarium graminarium])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eic OCA], [http://pdbe.org/2eic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eic RCSB], [http://www.ebi.ac.uk/pdbsum/2eic PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eic OCA], [http://pdbe.org/2eic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eic RCSB], [http://www.ebi.ac.uk/pdbsum/2eic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2eic ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/2eic_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/2eic_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Fusarium ~~graminearum~~]]	+	[[Category: Fusarium graminarium]]
	[[Category: Galactose oxidase]]		[[Category: Galactose oxidase]]
	[[Category: Akyumani, N]]		[[Category: Akyumani, N]]

OCA at 01:19, 9 February 2016

2016-02-09T01:19:29Z

←Older revision		Revision as of 01:19, 9 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eic ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:13, 10 September 2015

2015-09-10T17:13:04Z

←Older revision		Revision as of 17:13, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2eic' size='340' side='right' caption='[[2eic]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2eic' size='340' side='right' caption='[[2eic]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2eic]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Gibberella_zeae Gibberella zeae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2eic]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_graminearum Fusarium graminearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2eib\|2eib]], [[2eid\|2eid]], [[2eie\|2eie]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2eib\|2eib]], [[2eid\|2eid]], [[2eie\|2eie]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5518 ~~Gibberella zeae~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5518 Fusarium graminearum])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eic RCSB], [http://www.ebi.ac.uk/pdbsum/2eic PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eic OCA], [http://pdbe.org/2eic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eic RCSB], [http://www.ebi.ac.uk/pdbsum/2eic PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2eic" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Fusarium graminearum]]
	[[Category: Galactose oxidase]]		[[Category: Galactose oxidase]]
-	[[Category: Gibberella zeae]]
	[[Category: Akyumani, N]]		[[Category: Akyumani, N]]
	[[Category: Firbank, S J]]		[[Category: Firbank, S J]]

OCA at 18:11, 15 January 2015

2015-01-15T18:11:02Z

←Older revision		Revision as of 18:11, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eic]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gibberella_zeae Gibberella zeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eic]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gibberella_zeae Gibberella zeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2eib\|2eib]], [[2eid\|2eid]], [[2eie\|2eie]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2eib\|2eib]], [[2eid\|2eid]], [[2eie\|2eie]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5518 Gibberella zeae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5518 Gibberella zeae])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eic RCSB], [http://www.ebi.ac.uk/pdbsum/2eic PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eic RCSB], [http://www.ebi.ac.uk/pdbsum/2eic PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: Galactose oxidase]]		[[Category: Galactose oxidase]]
	[[Category: Gibberella zeae]]		[[Category: Gibberella zeae]]
-	[[Category: Akyumani, N.]]	+	[[Category: Akyumani, N]]
-	[[Category: Firbank, S J.]]	+	[[Category: Firbank, S J]]
-	[[Category: Knowles, P F.]]	+	[[Category: Knowles, P F]]
-	[[Category: McPherson, M J.]]	+	[[Category: McPherson, M J]]
-	[[Category: Phillips, S E.]]	+	[[Category: Phillips, S E]]
-	[[Category: Tamber, S.]]	+	[[Category: Tamber, S]]
	[[Category: Galactose oxidase w290f mutant]]		[[Category: Galactose oxidase w290f mutant]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 03:04, 29 September 2014

2014-09-29T03:04:04Z

←Older revision		Revision as of 03:04, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2eic.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of Galactose Oxidase mutant W290F==
		+	<StructureSection load='2eic' size='340' side='right' caption='[[2eic]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2eic]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gibberella_zeae Gibberella zeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2eib\|2eib]], [[2eid\|2eid]], [[2eie\|2eie]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5518 Gibberella zeae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactose_oxidase Galactose oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.9 1.1.3.9] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eic RCSB], [http://www.ebi.ac.uk/pdbsum/2eic PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/2eic_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The function of the stacking tryptophan, W290, a second-coordination sphere residue in galactose oxidase, has been investigated via steady-state kinetics measurements, absorption, CD and EPR spectroscopy, and X-ray crystallography of the W290F, W290G, and W290H variants. Enzymatic turnover is significantly slower in the W290 variants. The Km for D-galactose for W290H is similar to that of the wild type, whereas the Km is greatly elevated in W290G and W290F, suggesting a role for W290 in substrate binding and/or positioning via the NH group of the indole ring. Hydrogen bonding between W290 and azide in the wild type-azide crystal structure are consistent with this function. W290 modulates the properties and reactivity of the redox-active tyrosine radical; the Y272 tyrosyl radicals in both the W290G and W290H variants have elevated redox potentials and are highly unstable compared to the radical in W290F, which has properties similar to those of the wild-type tyrosyl radical. W290 restricts the accessibility of the Y272 radical site to solvent. Crystal structures show that Y272 is significantly more solvent exposed in the W290G variant but that W290F limits solvent access comparable to the wild-type indole side chain. Spectroscopic studies indicate that the Cu(II) ground states in the semireduced W290 variants are very similar to that of the wild-type protein. In addition, the electronic structures of W290X-azide complexes are also closely similar to the wild-type electronic structure. Azide binding and azide-mediated proton uptake by Y495 are perturbed in the variants, indicating that tryptophan also modulates the function of the catalytic base (Y495) in the wild-type enzyme. Thus, W290 plays multiple critical roles in enzyme catalysis, affecting substrate binding, the tyrosyl radical redox potential and stability, and the axial tyrosine function.

-	~~{{STRUCTURE_2eic\| PDB=2eic \| SCENE= }}~~	+	The stacking tryptophan of galactose oxidase: a second-coordination sphere residue that has profound effects on tyrosyl radical behavior and enzyme catalysis.,Rogers MS, Tyler EM, Akyumani N, Kurtis CR, Spooner RK, Deacon SE, Tamber S, Firbank SJ, Mahmoud K, Knowles PF, Phillips SE, McPherson MJ, Dooley DM Biochemistry. 2007 Apr 17;46(15):4606-18. Epub 2007 Mar 27. PMID:17385891<ref>PMID:17385891</ref>

-	~~===Crystal Structure~~ of ~~Galactose Oxidase mutant W290F===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17385891}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2eic]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Gibberella_zeae Gibberella zeae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA].~~	+

	==See Also==		==See Also==
	*[[Galactose oxidase\|Galactose oxidase]]		*[[Galactose oxidase\|Galactose oxidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:017385891<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Galactose oxidase]]		[[Category: Galactose oxidase]]
	[[Category: Gibberella zeae]]		[[Category: Gibberella zeae]]

OCA at 12:08, 20 October 2012

2012-10-20T12:08:50Z

←Older revision		Revision as of 12:08, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2eic]] is a 1 chain structure ~~of [[Galactose oxidase]]~~ with sequence from [http://en.wikipedia.org/wiki/Gibberella_zeae Gibberella zeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA].	+	[[2eic]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gibberella_zeae Gibberella zeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIC OCA].

	==See Also==		==See Also==

OCA: Protected "2eic" [edit=sysop:move=sysop]

2012-07-26T07:46:23Z

Protected "2eic" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:46, 26 July 2012