2ejg - Revision history

OCA at 08:41, 25 October 2023

2023-10-25T08:41:56Z

←Older revision		Revision as of 08:41, 25 October 2023
Line 3:		Line 3:
	<StructureSection load='2ejg' size='340' side='right'caption='[[2ejg]], [[Resolution\|resolution]] 2.71Å' scene=''>		<StructureSection load='2ejg' size='340' side='right'caption='[[2ejg]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ejg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Pyrococcus_horikoshii~~ Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EJG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ejg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EJG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>~~, ~~<scene name='pdbligand=BTN:BIOTIN'>BTN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.71Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2ejf\|2ejf]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td><td class="sblockDat">bpl ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii]), bccp ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ejg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejg OCA], [https://pdbe.org/2ejg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ejg RCSB], [https://www.ebi.ac.uk/pdbsum/2ejg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ejg ProSAT], [https://www.topsan.org/Proteins/RSGI/2ejg TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ejg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejg OCA], [https://pdbe.org/2ejg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ejg RCSB], [https://www.ebi.ac.uk/pdbsum/2ejg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ejg ProSAT], [https://www.topsan.org/Proteins/RSGI/2ejg TOPSAN]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O57883_PYRHO O57883_PYRHO]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Pyrococcus horikoshii]]	+	[[Category: Pyrococcus horikoshii OT3]]
-	[[Category: Bagautdinov, B]]	+	[[Category: Bagautdinov B]]
-	[[Category: Bagautdinova, S]]	+	[[Category: Bagautdinova S]]
-	[[Category: Kunishima, N]]	+	[[Category: Kunishima N]]
-	[[Category: Matsuura, Y]]	+	[[Category: Matsuura Y]]
-	~~[[Category: Structural genomic]]~~	+
-	~~[[Category: Biotinylation]]~~	+
-	~~[[Category: Dimer]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: National project on protein structural and functional analyse]]~~	+
-	~~[[Category: Nppsfa]]~~	+
-	~~[[Category: Rsgi~~]]	+

OCA at 12:09, 10 February 2021

2021-02-10T12:09:38Z

←Older revision		Revision as of 12:09, 10 February 2021
Line 1:		Line 1:

	==Crystal Structure Of The Biotin Protein Ligase (Mutation R48A) and Biotin Carboxyl Carrier Protein Complex From Pyrococcus Horikoshii OT3==		==Crystal Structure Of The Biotin Protein Ligase (Mutation R48A) and Biotin Carboxyl Carrier Protein Complex From Pyrococcus Horikoshii OT3==
-	<StructureSection load='2ejg' size='340' side='right' caption='[[2ejg]], [[Resolution\|resolution]] 2.71Å' scene=''>	+	<StructureSection load='2ejg' size='340' side='right'caption='[[2ejg]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ejg]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2EJG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ejg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EJG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ejf\|2ejf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ejf\|2ejf]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii]), bccp ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii]), bccp ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ejg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejg OCA], [~~http~~://pdbe.org/2ejg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ejg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ejg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ejg ProSAT], [~~http~~://www.topsan.org/Proteins/RSGI/2ejg TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ejg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejg OCA], [https://pdbe.org/2ejg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ejg RCSB], [https://www.ebi.ac.uk/pdbsum/2ejg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ejg ProSAT], [https://www.topsan.org/Proteins/RSGI/2ejg TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Biotin Protein Ligase\|Biotin Protein Ligase]]	+	*[[Biotin Protein Ligase 3D structures\|Biotin Protein Ligase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
	[[Category: Bagautdinov, B]]		[[Category: Bagautdinov, B]]

OCA at 08:16, 25 July 2018

2018-07-25T08:16:39Z

←Older revision		Revision as of 08:16, 25 July 2018
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ej/2ejg_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ej/2ejg_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 07:03, 18 October 2016

2016-10-18T07:03:26Z

←Older revision		Revision as of 07:03, 18 October 2016
Line 1:		Line 1:
		+
	==Crystal Structure Of The Biotin Protein Ligase (Mutation R48A) and Biotin Carboxyl Carrier Protein Complex From Pyrococcus Horikoshii OT3==		==Crystal Structure Of The Biotin Protein Ligase (Mutation R48A) and Biotin Carboxyl Carrier Protein Complex From Pyrococcus Horikoshii OT3==
	<StructureSection load='2ejg' size='340' side='right' caption='[[2ejg]], [[Resolution\|resolution]] 2.71Å' scene=''>		<StructureSection load='2ejg' size='340' side='right' caption='[[2ejg]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ejg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pyrho Pyrho~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EJG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ejg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EJG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ejf\|2ejf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ejf\|2ejf]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 ~~PYRHO~~]), bccp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 ~~PYRHO~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii]), bccp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ejg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejg OCA], [http://pdbe.org/2ejg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ejg RCSB], [http://www.ebi.ac.uk/pdbsum/2ejg PDBsum], [http://www.topsan.org/Proteins/RSGI/2ejg TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ejg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejg OCA], [http://pdbe.org/2ejg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ejg RCSB], [http://www.ebi.ac.uk/pdbsum/2ejg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ejg ProSAT], [http://www.topsan.org/Proteins/RSGI/2ejg TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pyrho~~]]	+	[[Category: Pyrococcus horikoshii]]
	[[Category: Bagautdinov, B]]		[[Category: Bagautdinov, B]]
	[[Category: Bagautdinova, S]]		[[Category: Bagautdinova, S]]

OCA at 06:25, 9 February 2016

2016-02-09T06:25:35Z

←Older revision		Revision as of 06:25, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ejg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 19:29, 10 September 2015

2015-09-10T19:29:03Z

←Older revision		Revision as of 19:29, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2ejg' size='340' side='right' caption='[[2ejg]], [[Resolution\|resolution]] 2.71Å' scene=''>		<StructureSection load='2ejg' size='340' side='right' caption='[[2ejg]], [[Resolution\|resolution]] 2.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ejg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pyrococcus_horikoshii Pyrococcus horikoshii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EJG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ejg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrho Pyrho]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EJG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ejf\|2ejf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ejf\|2ejf]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~53953 Pyrococcus horikoshii~~]), bccp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~53953 Pyrococcus horikoshii~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 PYRHO]), bccp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 PYRHO])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ejg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ejg RCSB], [http://www.ebi.ac.uk/pdbsum/2ejg PDBsum], [http://www.topsan.org/Proteins/RSGI/2ejg TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ejg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejg OCA], [http://pdbe.org/2ejg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ejg RCSB], [http://www.ebi.ac.uk/pdbsum/2ejg PDBsum], [http://www.topsan.org/Proteins/RSGI/2ejg TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ejg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pyrococcus horikoshii~~]]	+	[[Category: Pyrho]]
	[[Category: Bagautdinov, B]]		[[Category: Bagautdinov, B]]
	[[Category: Bagautdinova, S]]		[[Category: Bagautdinova, S]]

OCA at 16:47, 15 January 2015

2015-01-15T16:47:43Z

←Older revision		Revision as of 16:47, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ejg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EJG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ejg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EJG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ejf\|2ejf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ejf\|2ejf]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii]), bccp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii]), bccp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ejg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ejg RCSB], [http://www.ebi.ac.uk/pdbsum/2ejg PDBsum], [http://www.topsan.org/Proteins/RSGI/2ejg TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ejg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ejg RCSB], [http://www.ebi.ac.uk/pdbsum/2ejg PDBsum], [http://www.topsan.org/Proteins/RSGI/2ejg TOPSAN]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
-	[[Category: Bagautdinov, B.]]	+	[[Category: Bagautdinov, B]]
-	[[Category: Bagautdinova, S.]]	+	[[Category: Bagautdinova, S]]
-	[[Category: Kunishima, N.]]	+	[[Category: Kunishima, N]]
-	[[Category: Matsuura, Y.]]	+	[[Category: Matsuura, Y]]
-	[[Category: ~~RSGI, RIKEN~~ Structural ~~Genomics/Proteomics Initiative.~~]]	+	[[Category: Structural genomic]]
	[[Category: Biotinylation]]		[[Category: Biotinylation]]
	[[Category: Dimer]]		[[Category: Dimer]]
Line 44:		Line 44:
	[[Category: National project on protein structural and functional analyse]]		[[Category: National project on protein structural and functional analyse]]
	[[Category: Nppsfa]]		[[Category: Nppsfa]]
-	[[Category: Riken structural genomics/proteomics initiative]]
	[[Category: Rsgi]]		[[Category: Rsgi]]
-	[[Category: Structural genomic]]

OCA at 04:42, 29 September 2014

2014-09-29T04:42:18Z

←Older revision		Revision as of 04:42, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2ejg.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure Of The Biotin Protein Ligase (Mutation R48A) and Biotin Carboxyl Carrier Protein Complex From Pyrococcus Horikoshii OT3==
		+	<StructureSection load='2ejg' size='340' side='right' caption='[[2ejg]], [[Resolution\|resolution]] 2.71Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ejg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EJG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ejf\|2ejf]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii]), bccp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ejg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ejg RCSB], [http://www.ebi.ac.uk/pdbsum/2ejg PDBsum], [http://www.topsan.org/Proteins/RSGI/2ejg TOPSAN]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ej/2ejg_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Biotin protein ligase (BPL) catalyzes the biotinylation of the biotin carboxyl carrier protein (BCCP) only at a special lysine residue. Here we report the first structure of BPL.BCCP complex crystals, which are prepared using two BPL mutants: R48A and R48A/K111A. From a detailed structural characterization, it is likely that the mutants retain functionality as enzymes but have a reduced activity to produce the reaction intermediate biotinyl-5'-AMP. The observed biotin and partly disordered ATP in the mutant structures may act as a non-reactive analog of the substrates or biotinyl-5'-AMP, thereby providing the complex crystals. The four crystallographically independent BPL.BCCP complexes obtained can be classified structurally into three groups: the formation stages 1 and 2 with apo-BCCP and the product stage with biotinylated holo-BCCP. Residues responsible for the complex formation as well as for the biotinylation reaction have been identified. The C-terminal domain of BPL shows especially large conformational changes to accommodate BCCP, suggesting its functional importance. The formation stage 1 complex shows the closest distance between the carboxyl carbon of biotin and the special lysine of BCCP, suggesting its relevance to the unobserved reaction stage. Interestingly, bound ATP and biotin are also seen in the product stage, indicating that the substrates may be recruited into the product stage complex before the release of holo-BCCP, probably for the next reaction cycle. The existence of formation and product stages before and after the reaction stage would be favorable to ensure both the reaction efficiency and the extreme substrate specificity of the biotinylation reaction.

-	~~{{STRUCTURE_2ejg\| PDB=2ejg \| SCENE= }}~~	+	Protein biotinylation visualized by a complex structure of biotin protein ligase with a substrate.,Bagautdinov B, Matsuura Y, Bagautdinova S, Kunishima N J Biol Chem. 2008 May 23;283(21):14739-50. Epub 2008 Mar 26. PMID:18372281<ref>PMID:18372281</ref>

-	~~===Crystal Structure Of The Biotin Protein Ligase (Mutation R48A) and Biotin Carboxyl Carrier Protein Complex~~ From ~~Pyrococcus Horikoshii OT3===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18372281}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2ejg]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA].~~	+

	==See Also==		==See Also==
	*[[Biotin Protein Ligase\|Biotin Protein Ligase]]		*[[Biotin Protein Ligase\|Biotin Protein Ligase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:018372281<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
	[[Category: Bagautdinov, B.]]		[[Category: Bagautdinov, B.]]

OCA at 12:57, 20 October 2012

2012-10-20T12:57:24Z

←Older revision		Revision as of 12:57, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2ejg]] is a 4 chain structure ~~of [[Biotin Protein Ligase]]~~ with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA].	+	[[2ejg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJG OCA].

	==See Also==		==See Also==

OCA: Protected "2ejg" [edit=sysop:move=sysop]

2012-07-26T10:27:23Z

Protected "2ejg" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:27, 26 July 2012