2eot - Revision history

OCA at 15:56, 22 December 2021

2021-12-22T15:56:41Z

←Older revision		Revision as of 15:56, 22 December 2021
Line 3:		Line 3:
	<StructureSection load='2eot' size='340' side='right'caption='[[2eot]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>		<StructureSection load='2eot' size='340' side='right'caption='[[2eot]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2eot]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2EOT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2eot]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EOT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2eot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eot OCA], [~~http~~://pdbe.org/2eot PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2eot RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2eot PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2eot ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eot OCA], [https://pdbe.org/2eot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eot RCSB], [https://www.ebi.ac.uk/pdbsum/2eot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eot ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CCL11_HUMAN CCL11_HUMAN]] In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.	+	[[https://www.uniprot.org/uniprot/CCL11_HUMAN CCL11_HUMAN]] In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:40, 18 March 2020

2020-03-18T10:40:29Z

←Older revision		Revision as of 10:40, 18 March 2020
Line 1:		Line 1:

	==SOLUTION STRUCTURE OF EOTAXIN, AN ENSEMBLE OF 32 NMR SOLUTION STRUCTURES==		==SOLUTION STRUCTURE OF EOTAXIN, AN ENSEMBLE OF 32 NMR SOLUTION STRUCTURES==
-	<StructureSection load='2eot' size='340' side='right' caption='[[2eot]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>	+	<StructureSection load='2eot' size='340' side='right'caption='[[2eot]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EOT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EOT FirstGlance]. <br>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Clark-Lewis, I]]		[[Category: Clark-Lewis, I]]
	[[Category: Crump, M P]]		[[Category: Crump, M P]]

OCA at 09:26, 14 June 2018

2018-06-14T09:26:09Z

←Older revision		Revision as of 09:26, 14 June 2018
Line 1:		Line 1:
		+
	==SOLUTION STRUCTURE OF EOTAXIN, AN ENSEMBLE OF 32 NMR SOLUTION STRUCTURES==		==SOLUTION STRUCTURE OF EOTAXIN, AN ENSEMBLE OF 32 NMR SOLUTION STRUCTURES==
	<StructureSection load='2eot' size='340' side='right' caption='[[2eot]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>		<StructureSection load='2eot' size='340' side='right' caption='[[2eot]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EOT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EOT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eot OCA], [http://pdbe.org/2eot PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eot RCSB], [http://www.ebi.ac.uk/pdbsum/2eot PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eot OCA], [http://pdbe.org/2eot PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eot RCSB], [http://www.ebi.ac.uk/pdbsum/2eot PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2eot ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/2eot_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/2eot_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 17:42, 8 February 2016

2016-02-08T17:42:48Z

←Older revision		Revision as of 17:42, 8 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eot ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:55, 10 September 2015

2015-09-10T13:55:45Z

←Older revision		Revision as of 13:55, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2eot' size='340' side='right' caption='[[2eot]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>		<StructureSection load='2eot' size='340' side='right' caption='[[2eot]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2eot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EOT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2eot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EOT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eot OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eot RCSB], [http://www.ebi.ac.uk/pdbsum/2eot PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eot OCA], [http://pdbe.org/2eot PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eot RCSB], [http://www.ebi.ac.uk/pdbsum/2eot PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2eot" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Clark-Lewis, I]]		[[Category: Clark-Lewis, I]]
	[[Category: Crump, M P]]		[[Category: Crump, M P]]

OCA at 01:03, 25 December 2014

2014-12-25T01:03:42Z

←Older revision		Revision as of 01:03, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EOT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EOT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eot OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eot RCSB], [http://www.ebi.ac.uk/pdbsum/2eot PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eot OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eot RCSB], [http://www.ebi.ac.uk/pdbsum/2eot PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CCL11_HUMAN CCL11_HUMAN]] In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Clark-Lewis, I.]]	+	[[Category: Clark-Lewis, I]]
-	[[Category: Crump, M P.]]	+	[[Category: Crump, M P]]
-	[[Category: Kim, K S.]]	+	[[Category: Kim, K S]]
-	[[Category: Rajarathnam, K.]]	+	[[Category: Rajarathnam, K]]
-	[[Category: Sykes, B D.]]	+	[[Category: Sykes, B D]]
	[[Category: Ccr3]]		[[Category: Ccr3]]
	[[Category: Chemokine]]		[[Category: Chemokine]]

OCA: Protected "2eot" [edit=sysop:move=sysop]

2014-09-29T04:55:49Z

Protected "2eot" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:55, 29 September 2014

OCA at 04:55, 29 September 2014

2014-09-29T04:55:47Z

←Older revision		Revision as of 04:55, 29 September 2014
Line 1:		Line 1:
-	~~{{Seed}}~~	+	==SOLUTION STRUCTURE OF EOTAXIN, AN ENSEMBLE OF 32 NMR SOLUTION STRUCTURES==
-	[[~~Image~~:2eot.~~png\|left~~\|200px]]	+	<StructureSection load='2eot' size='340' side='right' caption='[[2eot]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2eot]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EOT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eot OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eot RCSB], [http://www.ebi.ac.uk/pdbsum/2eot PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/2eot_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The solution structure of the CCR3-specific chemokine, eotaxin, has been determined by NMR spectroscopy. The quaternary structure of eotaxin was investigated by ultracentrifugation and NMR, and it was found to be in equilibrium between monomer and dimer under a wide range of conditions. At pH </= 5 and low ionic strength, eotaxin was found to be predominantly a monomer. The three-dimensional structure of the eotaxin monomer solved at pH 5.0 revealed that it has a typical chemokine fold, which includes a 3-stranded beta-sheet and an overlying alpha-helix. Except for the N-terminal residues (residues 1-8), the core of the protein is well defined. The eotaxin structure is compared with the chemokines regulated upon activation, normal T-cell expressed and secreted (RANTES) and monocyte chemoattractant protein-1 (MCP-1); eotaxin binds only CC chemokine receptor CCR3, whereas RANTES binds many receptors including CCR3, and MCP-1 binds a distinct receptor, CCR2. The RMSD of the eotaxin ensemble of structures with the RANTES average minimized monomeric subunit is 5.52 +/- 0.87 A over all backbone atoms and 1.14 +/- 0.09 A over backbone atoms of residues 11-28 and 34-65. The most important difference between the structures is in the N-terminal residues that are unstructured in eotaxin but structured in RANTES and MCP-1. Several residues in the loop region of RANTES show similar packing in eotaxin (residues 11-17). As the N-terminal and loop regions have been shown to be critical for receptor binding and signaling, this structure will be useful for determining the basis for CCR3 selectivity of the eotaxin.

-	~~<!--~~	+	Solution structure of eotaxin, a chemokine that selectively recruits eosinophils in allergic inflammation.,Crump MP, Rajarathnam K, Kim KS, Clark-Lewis I, Sykes BD J Biol Chem. 1998 Aug 28;273(35):22471-9. PMID:9712872<ref>PMID:9712872</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_2eot"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_2eot\| PDB=2eot \| SCENE= }}~~	+

-	~~===SOLUTION STRUCTURE OF EOTAXIN~~, ~~AN ENSEMBLE OF 32 NMR SOLUTION STRUCTURES===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_9712872}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 9712872 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_9712872}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~2EOT is a 1 chain structure of sequence from [http:~~/~~/en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:9712872<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Clark-Lewis, I.]]		[[Category: Clark-Lewis, I.]]
Line 37:		Line 40:
	[[Category: Protein synthesis]]		[[Category: Protein synthesis]]
	[[Category: Solution structure]]		[[Category: Solution structure]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:52:33 2009''

OCA at 22:52, 16 February 2009

2009-02-16T22:52:35Z

←Older revision		Revision as of 22:52, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2EOT is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA].	+	2EOT is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA].

	==Reference==		==Reference==
-	Solution structure of eotaxin, a chemokine that selectively recruits eosinophils in allergic inflammation., Crump MP, Rajarathnam K, Kim KS, Clark-Lewis I, Sykes BD, J Biol Chem. 1998 Aug 28;273(35):22471-9. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/9712872 9712872]~~	+	<ref group="xtra">PMID:9712872</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Single protein]]
	[[Category: Clark-Lewis, I.]]		[[Category: Clark-Lewis, I.]]
	[[Category: Crump, M P.]]		[[Category: Crump, M P.]]
Line 39:		Line 38:
	[[Category: Solution structure]]		[[Category: Solution structure]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 05~~:23:~~50 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:52:33 2009''

OCA at 02:23, 28 July 2008

2008-07-28T02:23:56Z

←Older revision		Revision as of 02:23, 28 July 2008
Line 1:		Line 1:
-	[[Image:2eot.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2eot.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2eot\| PDB=2eot \| SCENE= }}		{{STRUCTURE_2eot\| PDB=2eot \| SCENE= }}

-	~~'''~~SOLUTION STRUCTURE OF EOTAXIN, AN ENSEMBLE OF 32 NMR SOLUTION STRUCTURES~~'''~~	+	===SOLUTION STRUCTURE OF EOTAXIN, AN ENSEMBLE OF 32 NMR SOLUTION STRUCTURES===


-	~~==Overview==~~	+	<!--
-	The ~~solution structure of the CCR3-specific chemokine~~, ~~eotaxin~~, ~~has been determined by NMR spectroscopy. The quaternary structure of eotaxin was investigated by ultracentrifugation and NMR, and it was found~~ to ~~be in equilibrium between monomer and dimer under a wide range of conditions. At pH </= 5 and low ionic strength, eotaxin was found to be predominantly a monomer. The three-dimensional structure of~~ the ~~eotaxin monomer solved~~ at ~~pH 5~~.~~0 revealed that it has a typical chemokine fold, which includes a 3-stranded beta-sheet and an overlying alpha-helix~~. ~~Except for the N-terminal residues (residues 1-8~~), ~~the core of the protein~~ is ~~well defined. The eotaxin structure is compared with~~ the chemokines regulated upon activation, normal T-cell expressed and secreted (RANTES) and monocyte chemoattractant protein-1 (MCP-1); eotaxin binds only CC chemokine receptor CCR3, whereas RANTES binds many receptors including CCR3, and MCP-1 binds a distinct receptor, CCR2. ~~The RMSD of the eotaxin ensemble of structures with the RANTES average minimized monomeric subunit is 5.52 +/~~- ~~0.87 A over all backbone atoms and 1.14 +/~~- 0.09 A over backbone atoms of residues 11-28 and 34-65. The most important difference between the structures is in the N-terminal residues that are unstructured in eotaxin but structured in RANTES and MCP-1. Several residues in the loop region of RANTES show similar packing in eotaxin (residues 11-17). As the N-terminal and loop regions have been shown to be critical for receptor binding and signaling, this structure will be useful for determining the basis for CCR3 selectivity of the eotaxin.	+	The line below this paragraph, {{ABSTRACT_PUBMED_9712872}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 9712872 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_9712872}}

	==About this Structure==		==About this Structure==
-	2EOT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA].	+	2EOT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EOT OCA].

	==Reference==		==Reference==
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	[[Category: Protein synthesis]]		[[Category: Protein synthesis]]
	[[Category: Solution structure]]		[[Category: Solution structure]]
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