2f8q - Revision history

OCA at 09:22, 30 August 2023

2023-08-30T09:22:55Z

←Older revision		Revision as of 09:22, 30 August 2023
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	<StructureSection load='2f8q' size='340' side='right'caption='[[2f8q]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2f8q' size='340' side='right'caption='[[2f8q]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2f8q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp.~~_ng~~-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F8Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2f8q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._NG-27 Bacillus sp. NG-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F8Q FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8q OCA], [https://pdbe.org/2f8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f8q RCSB], [https://www.ebi.ac.uk/pdbsum/2f8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8q OCA], [https://pdbe.org/2f8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f8q RCSB], [https://www.ebi.ac.uk/pdbsum/2f8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8q ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O30700_9BACI O30700_9BACI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus sp. ng-27]]	+	[[Category: Bacillus sp. NG-27]]
-	~~[[Category: Endo-1,4-beta-xylanase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bhardwaj, A]]	+	[[Category: Bhardwaj A]]
-	[[Category: Ghosh, A]]	+	[[Category: Ghosh A]]
-	[[Category: Manikandan, K]]	+	[[Category: Manikandan K]]
-	[[Category: Ramakumar, S]]	+	[[Category: Ramakumar S]]
-	[[Category: Reddy~~, V S]]~~	+	[[Category: Reddy VS]]
-	~~[[Category: Alkali thermostable family 10 xylanase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 09:53, 5 May 2021

2021-05-05T09:53:32Z

←Older revision		Revision as of 09:53, 5 May 2021
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	<StructureSection load='2f8q' size='340' side='right'caption='[[2f8q]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2f8q' size='340' side='right'caption='[[2f8q]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2f8q]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacillus_sp._ng-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2F8Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2f8q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._ng-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F8Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8q OCA], [~~http~~://pdbe.org/2f8q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2f8q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2f8q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8q OCA], [https://pdbe.org/2f8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f8q RCSB], [https://www.ebi.ac.uk/pdbsum/2f8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8q ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 09:20, 24 July 2019

2019-07-24T09:20:59Z

←Older revision		Revision as of 09:20, 24 July 2019
Line 1:		Line 1:

	==An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27==		==An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27==
-	<StructureSection load='2f8q' size='340' side='right' caption='[[2f8q]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='2f8q' size='340' side='right'caption='[[2f8q]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f8q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._ng-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F8Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f8q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._ng-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F8Q FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f8/2f8q_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f8/2f8q_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 33:
	[[Category: Bacillus sp. ng-27]]		[[Category: Bacillus sp. ng-27]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
		+	[[Category: Large Structures]]
	[[Category: Bhardwaj, A]]		[[Category: Bhardwaj, A]]
	[[Category: Ghosh, A]]		[[Category: Ghosh, A]]

OCA at 07:35, 18 October 2017

2017-10-18T07:35:17Z

←Older revision		Revision as of 07:35, 18 October 2017
Line 1:		Line 1:
		+
	==An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27==		==An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27==
	<StructureSection load='2f8q' size='340' side='right' caption='[[2f8q]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2f8q' size='340' side='right' caption='[[2f8q]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8q OCA], [http://pdbe.org/2f8q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f8q RCSB], [http://www.ebi.ac.uk/pdbsum/2f8q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8q OCA], [http://pdbe.org/2f8q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f8q RCSB], [http://www.ebi.ac.uk/pdbsum/2f8q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8q ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 17:12, 9 February 2016

2016-02-09T17:12:36Z

←Older revision		Revision as of 17:12, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f8q ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:23, 11 September 2015

2015-09-11T00:23:29Z

←Older revision		Revision as of 00:23, 11 September 2015
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f8q RCSB], [http://www.ebi.ac.uk/pdbsum/2f8q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8q OCA], [http://pdbe.org/2f8q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f8q RCSB], [http://www.ebi.ac.uk/pdbsum/2f8q PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2f8q" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:24, 16 January 2015

2015-01-16T08:24:03Z

←Older revision		Revision as of 08:24, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f8q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._ng-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F8Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f8q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._ng-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F8Q FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f8q RCSB], [http://www.ebi.ac.uk/pdbsum/2f8q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f8q RCSB], [http://www.ebi.ac.uk/pdbsum/2f8q PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	[[Category: Bacillus sp. ng-27]]		[[Category: Bacillus sp. ng-27]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
-	[[Category: Bhardwaj, A.]]	+	[[Category: Bhardwaj, A]]
-	[[Category: Ghosh, A.]]	+	[[Category: Ghosh, A]]
-	[[Category: Manikandan, K.]]	+	[[Category: Manikandan, K]]
-	[[Category: Ramakumar, S.]]	+	[[Category: Ramakumar, S]]
-	[[Category: Reddy, V S.]]	+	[[Category: Reddy, V S]]
	[[Category: Alkali thermostable family 10 xylanase]]		[[Category: Alkali thermostable family 10 xylanase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 07:17, 10 September 2014

2014-09-10T07:17:31Z

←Older revision		Revision as of 07:17, 10 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2f8q.~~png\|left~~\|200px]]	+	==An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27==
		+	<StructureSection load='2f8q' size='340' side='right' caption='[[2f8q]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2f8q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._ng-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F8Q FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f8q RCSB], [http://www.ebi.ac.uk/pdbsum/2f8q PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f8/2f8q_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Crystal structures are known for several glycosyl hydrolase family 10 (GH10) xylanases. However, none of them is from an alkalophilic organism that can grow in alkaline conditions. We have determined the crystal structures at 2.2 Angstroms of a GH10 extracellular endoxylanase (BSX) from an alkalophilic Bacillus sp. NG-27, for the native and the complex enzyme with xylosaccharides. The industrially important enzyme is optimally active and stable at 343 K and at a pH of 8.4. Comparison of the structure of BSX with those of other thermostable GH10 xylanases optimally active at acidic or close to neutral pH showed that the solvent-exposed acidic amino acids, Asp and Glu, are markedly enhanced in BSX, while solvent-exposed Asn was noticeably depleted. The BSX crystal structure when compared with putative three-dimensional homology models of other extracellular alkalophilic GH10 xylanases from alkalophilic organisms suggests that a protein surface rich in acidic residues may be an important feature common to these alkali thermostable enzymes. A comparison of the surface features of BSX and of halophilic proteins allowed us to predict the activity of BSX at high salt concentrations, which we verified through experiments. This offered us important lessons in the polyextremophilicity of proteins, where understanding the structural features of a protein stable in one set of extreme conditions provided clues about the activity of the protein in other extreme conditions. The work brings to the fore the role of the nature and composition of solvent-exposed residues in the adaptation of enzymes to polyextreme conditions, as in BSX.

-	~~{{STRUCTURE_2f8q\| PDB=2f8q \| SCENE= }}~~	+	Crystal structures of native and xylosaccharide-bound alkali thermostable xylanase from an alkalophilic Bacillus sp. NG-27: structural insights into alkalophilicity and implications for adaptation to polyextreme conditions.,Manikandan K, Bhardwaj A, Gupta N, Lokanath NK, Ghosh A, Reddy VS, Ramakumar S Protein Sci. 2006 Aug;15(8):1951-60. Epub 2006 Jul 5. PMID:16823036<ref>PMID:16823036</ref>

-	~~===An alkali thermostable F~~/~~10 xylanase from alkalophilic Bacillus sp. NG-27===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16823036}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2f8q]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_sp~~.~~_ng-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016823036<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bacillus sp. ng-27]]		[[Category: Bacillus sp. ng-27]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]

OCA: Protected "2f8q" [edit=sysop:move=sysop]

2013-01-07T11:50:10Z

Protected "2f8q" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:50, 7 January 2013

OCA at 11:50, 7 January 2013

2013-01-07T11:50:05Z

←Older revision		Revision as of 11:50, 7 January 2013
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-	{{Seed}}
	[[Image:2f8q.png\|left\|200px]]		[[Image:2f8q.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2f8q", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2f8q\| PDB=2f8q \| SCENE= }}		{{STRUCTURE_2f8q\| PDB=2f8q \| SCENE= }}

	===An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27===		===An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16823036}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16823036 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16823036}}		{{ABSTRACT_PUBMED_16823036}}

	==About this Structure==		==About this Structure==
-	~~2F8Q~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Bacteria Bacteria~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA].	+	[[2f8q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._ng-27 Bacillus sp. ng-27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Q OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16823036~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016823036</ref><references group="xtra"/>
-	[[Category: ~~Bacteria~~]]	+	[[Category: Bacillus sp. ng-27]]
	[[Category: Endo-1,4-beta-xylanase]]		[[Category: Endo-1,4-beta-xylanase]]
	[[Category: Bhardwaj, A.]]		[[Category: Bhardwaj, A.]]
Line 32:		Line 20:
	[[Category: Reddy, V S.]]		[[Category: Reddy, V S.]]
	[[Category: Alkali thermostable family 10 xylanase]]		[[Category: Alkali thermostable family 10 xylanase]]
-		+	[[Category: Hydrolase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 11:25:50 2009''~~	+