2fzv - Revision history

OCA at 09:25, 14 February 2024

2024-02-14T09:25:57Z

←Older revision		Revision as of 09:25, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='2fzv' size='340' side='right'caption='[[2fzv]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2fzv' size='340' side='right'caption='[[2fzv]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2fzv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri_2a_str.~~_2457t~~ Shigella flexneri 2a str. ~~2457t~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FZV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2fzv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri_2a_str._2457T Shigella flexneri 2a str. 2457T]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FZV FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~AAP17869 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=198215 Shigella flexneri 2a str. 2457T])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fzv OCA], [https://pdbe.org/2fzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fzv RCSB], [https://www.ebi.ac.uk/pdbsum/2fzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fzv ProSAT], [https://www.topsan.org/Proteins/MCSG/2fzv TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fzv OCA], [https://pdbe.org/2fzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fzv RCSB], [https://www.ebi.ac.uk/pdbsum/2fzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fzv ProSAT], [https://www.topsan.org/Proteins/MCSG/2fzv TOPSAN]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ARREH_SHIFL ARREH_SHIFL] Has NADPH-dependent FMN reductase activity and very low azoreductase activity. No activity with NADH.<ref>PMID:17962405</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fzv ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fzv ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The arsH gene or its homologs are a frequent part of the arsenic resistance system in bacteria and eukaryotes. Although a specific biological function of the gene product is unknown, the ArsH protein was annotated as a member of the NADPH-dependent FMN reductase family based on a conserved (T/S)XRXXSX(T/S) fingerprint motif common for FMN binding proteins. Presented here are the first crystal structure of an ArsH protein from Shigella flexneri refined at 1.7 A resolution and results of enzymatic activity assays that revealed a strong NADPH-dependent FMN reductase and low azoreductase activities. The ArsH apo protein has an alpha/beta/alpha-fold typical for FMN binding proteins. The asymmetric unit consists of four monomers, which form a tetramer. Buried surface analysis suggests that this tetramer is likely to be the relevant biological assembly. Dynamic light scattering experiments are consistent with this hypothesis and show that ArsH in solution at room temperature does exist predominantly in the tetrameric form.
-
-	Crystal structure of an apo form of Shigella flexneri ArsH protein with an NADPH-dependent FMN reductase activity.,Vorontsov II, Minasov G, Brunzelle JS, Shuvalova L, Kiryukhina O, Collart FR, Anderson WF Protein Sci. 2007 Nov;16(11):2483-90. PMID:17962405<ref>PMID:17962405</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2fzv" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Shigella flexneri 2a str. ~~2457t~~]]	+	[[Category: Shigella flexneri 2a str. 2457T]]
-	[[Category: Anderson~~, W F~~]]	+	[[Category: Anderson WF]]
-	[[Category: Brunzelle~~, J S~~]]	+	[[Category: Brunzelle JS]]
-	[[Category: Collart~~, F R~~]]	+	[[Category: Collart FR]]
-	[[Category: Joachimiak, A]]	+	[[Category: Joachimiak A]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Minasov G]]
-	[[Category: Minasov, G]]	+	[[Category: Shuvalova L]]
-	[[Category: Shuvalova, L]]	+	[[Category: Vorontsov II]]
-	[[Category: Vorontsov~~, I I]]~~	+
-	~~[[Category: Flavin binding protein]]~~	+
-	~~[[Category: Mcsg]]~~	+
-	~~[[Category: PSI, Protein structure initiative]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 15:36, 3 March 2021

2021-03-03T15:36:47Z

←Older revision		Revision as of 15:36, 3 March 2021
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	==Crystal Structure of an apo form of a Flavin-binding Protein from Shigella flexneri==		==Crystal Structure of an apo form of a Flavin-binding Protein from Shigella flexneri==
-	<StructureSection load='2fzv' size='340' side='right' caption='[[2fzv]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='2fzv' size='340' side='right'caption='[[2fzv]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2fzv]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Shigella_flexneri_2a_str._2457t Shigella flexneri 2a str. 2457t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2FZV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2fzv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri_2a_str._2457t Shigella flexneri 2a str. 2457t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FZV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAP17869 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198215 Shigella flexneri 2a str. 2457T])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAP17869 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198215 Shigella flexneri 2a str. 2457T])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2fzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fzv OCA], [~~http~~://pdbe.org/2fzv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2fzv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2fzv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2fzv ProSAT], [~~http~~://www.topsan.org/Proteins/MCSG/2fzv TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fzv OCA], [https://pdbe.org/2fzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fzv RCSB], [https://www.ebi.ac.uk/pdbsum/2fzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fzv ProSAT], [https://www.topsan.org/Proteins/MCSG/2fzv TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fz/2fzv_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fz/2fzv_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Shigella flexneri 2a str. 2457t]]		[[Category: Shigella flexneri 2a str. 2457t]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]

OCA at 07:46, 18 October 2017

2017-10-18T07:46:18Z

←Older revision		Revision as of 07:46, 18 October 2017
Line 1:		Line 1:
		+
	==Crystal Structure of an apo form of a Flavin-binding Protein from Shigella flexneri==		==Crystal Structure of an apo form of a Flavin-binding Protein from Shigella flexneri==
	<StructureSection load='2fzv' size='340' side='right' caption='[[2fzv]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2fzv' size='340' side='right' caption='[[2fzv]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAP17869 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198215 Shigella flexneri 2a str. 2457T])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAP17869 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198215 Shigella flexneri 2a str. 2457T])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fzv OCA], [http://pdbe.org/2fzv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fzv RCSB], [http://www.ebi.ac.uk/pdbsum/2fzv PDBsum], [http://www.topsan.org/Proteins/MCSG/2fzv TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fzv OCA], [http://pdbe.org/2fzv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fzv RCSB], [http://www.ebi.ac.uk/pdbsum/2fzv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fzv ProSAT], [http://www.topsan.org/Proteins/MCSG/2fzv TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 23:12, 9 February 2016

2016-02-09T23:12:08Z

←Older revision		Revision as of 23:12, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fzv ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:59, 11 September 2015

2015-09-11T02:59:22Z

←Older revision		Revision as of 02:59, 11 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAP17869 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198215 Shigella flexneri 2a str. 2457T])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAP17869 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198215 Shigella flexneri 2a str. 2457T])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fzv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fzv RCSB], [http://www.ebi.ac.uk/pdbsum/2fzv PDBsum], [http://www.topsan.org/Proteins/MCSG/2fzv TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fzv OCA], [http://pdbe.org/2fzv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fzv RCSB], [http://www.ebi.ac.uk/pdbsum/2fzv PDBsum], [http://www.topsan.org/Proteins/MCSG/2fzv TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2fzv" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:09, 25 December 2014

2014-12-25T10:09:52Z

←Older revision		Revision as of 10:09, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fzv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri_2a_str._2457t Shigella flexneri 2a str. 2457t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FZV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fzv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri_2a_str._2457t Shigella flexneri 2a str. 2457t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FZV FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAP17869 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198215 Shigella flexneri 2a str. 2457T])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAP17869 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198215 Shigella flexneri 2a str. 2457T])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fzv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fzv RCSB], [http://www.ebi.ac.uk/pdbsum/2fzv PDBsum], [http://www.topsan.org/Proteins/MCSG/2fzv TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fzv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fzv RCSB], [http://www.ebi.ac.uk/pdbsum/2fzv PDBsum], [http://www.topsan.org/Proteins/MCSG/2fzv TOPSAN]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Shigella flexneri 2a str. 2457t]]		[[Category: Shigella flexneri 2a str. 2457t]]
-	[[Category: Anderson, W F.]]	+	[[Category: Anderson, W F]]
-	[[Category: Brunzelle, J S.]]	+	[[Category: Brunzelle, J S]]
-	[[Category: Collart, F R.]]	+	[[Category: Collart, F R]]
-	[[Category: Joachimiak, A.]]	+	[[Category: Joachimiak, A]]
-	[[Category: ~~MCSG, Midwest Center for~~ Structural ~~Genomics.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Minasov, G.]]	+	[[Category: Minasov, G]]
-	[[Category: Shuvalova, L.]]	+	[[Category: Shuvalova, L]]
-	[[Category: Vorontsov, I I.]]	+	[[Category: Vorontsov, I I]]
	[[Category: Flavin binding protein]]		[[Category: Flavin binding protein]]
	[[Category: Mcsg]]		[[Category: Mcsg]]
-	[[Category: ~~Midwest center for structural genomic]]~~	+	[[Category: PSI, Protein structure initiative]]
-	~~[[Category:~~ Protein structure initiative]]	+
-	~~[[Category: Psi]]~~	+
-	~~[[Category: Structural genomic~~]]	+
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 09:12, 30 September 2014

2014-09-30T09:12:39Z

←Older revision		Revision as of 09:12, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2fzv.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of an apo form of a Flavin-binding Protein from Shigella flexneri==
		+	<StructureSection load='2fzv' size='340' side='right' caption='[[2fzv]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2fzv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri_2a_str._2457t Shigella flexneri 2a str. 2457t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FZV FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AAP17869 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198215 Shigella flexneri 2a str. 2457T])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fzv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fzv RCSB], [http://www.ebi.ac.uk/pdbsum/2fzv PDBsum], [http://www.topsan.org/Proteins/MCSG/2fzv TOPSAN]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fz/2fzv_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The arsH gene or its homologs are a frequent part of the arsenic resistance system in bacteria and eukaryotes. Although a specific biological function of the gene product is unknown, the ArsH protein was annotated as a member of the NADPH-dependent FMN reductase family based on a conserved (T/S)XRXXSX(T/S) fingerprint motif common for FMN binding proteins. Presented here are the first crystal structure of an ArsH protein from Shigella flexneri refined at 1.7 A resolution and results of enzymatic activity assays that revealed a strong NADPH-dependent FMN reductase and low azoreductase activities. The ArsH apo protein has an alpha/beta/alpha-fold typical for FMN binding proteins. The asymmetric unit consists of four monomers, which form a tetramer. Buried surface analysis suggests that this tetramer is likely to be the relevant biological assembly. Dynamic light scattering experiments are consistent with this hypothesis and show that ArsH in solution at room temperature does exist predominantly in the tetrameric form.

-	~~{{STRUCTURE_2fzv\| PDB=2fzv \| SCENE= }}~~	+	Crystal structure of an apo form of Shigella flexneri ArsH protein with an NADPH-dependent FMN reductase activity.,Vorontsov II, Minasov G, Brunzelle JS, Shuvalova L, Kiryukhina O, Collart FR, Anderson WF Protein Sci. 2007 Nov;16(11):2483-90. PMID:17962405<ref>PMID:17962405</ref>

-	~~===Crystal Structure~~ of ~~an apo form of a Flavin-binding Protein from Shigella flexneri===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17962405}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2fzv]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Shigella_flexneri_2a_str~~.~~_2457t Shigella flexneri 2a str. 2457t]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017962405<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Shigella flexneri 2a str. 2457t]]		[[Category: Shigella flexneri 2a str. 2457t]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F.]]

OCA: Protected "2fzv" [edit=sysop:move=sysop]

2013-01-07T14:07:55Z

Protected "2fzv" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:07, 7 January 2013

OCA at 14:07, 7 January 2013

2013-01-07T14:07:52Z

←Older revision		Revision as of 14:07, 7 January 2013
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-	{{Seed}}
	[[Image:2fzv.png\|left\|200px]]		[[Image:2fzv.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2fzv", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2fzv\| PDB=2fzv \| SCENE= }}		{{STRUCTURE_2fzv\| PDB=2fzv \| SCENE= }}

	===Crystal Structure of an apo form of a Flavin-binding Protein from Shigella flexneri===		===Crystal Structure of an apo form of a Flavin-binding Protein from Shigella flexneri===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17962405}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17962405 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17962405}}		{{ABSTRACT_PUBMED_17962405}}

	==About this Structure==		==About this Structure==
-	~~2FZV~~ is a 4 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Shigella_flexneri_2a_str._2457t Shigella flexneri 2a str. 2457t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA].	+	[[2fzv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri_2a_str._2457t Shigella flexneri 2a str. 2457t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17962405~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017962405</ref><references group="xtra"/>
	[[Category: Shigella flexneri 2a str. 2457t]]		[[Category: Shigella flexneri 2a str. 2457t]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F.]]
Line 40:		Line 28:
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Unknown function]]		[[Category: Unknown function]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 15:56:38 2010''

OCA at 11:50, 14 July 2010

2010-07-14T11:50:29Z

←Older revision		Revision as of 11:50, 14 July 2010
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2FZV is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/~~Shigella_flexneri_2a~~ Shigella flexneri 2a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA].	+	2FZV is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Shigella_flexneri_2a_str._2457t Shigella flexneri 2a str. 2457t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:17962405</ref><references group="xtra"/>		<ref group="xtra">PMID:17962405</ref><references group="xtra"/>
-	[[Category: Shigella flexneri 2a]]	+	[[Category: Shigella flexneri 2a str. 2457t]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F.]]
	[[Category: Brunzelle, J S.]]		[[Category: Brunzelle, J S.]]
	[[Category: Collart, F R.]]		[[Category: Collart, F R.]]
		+	[[Category: Joachimiak, A.]]
	[[Category: MCSG, Midwest Center for Structural Genomics.]]		[[Category: MCSG, Midwest Center for Structural Genomics.]]
	[[Category: Minasov, G.]]		[[Category: Minasov, G.]]
	[[Category: Shuvalova, L.]]		[[Category: Shuvalova, L.]]
	[[Category: Vorontsov, I I.]]		[[Category: Vorontsov, I I.]]
-	[[Category: Crystal structure]]
	[[Category: Flavin binding protein]]		[[Category: Flavin binding protein]]
	[[Category: Mcsg]]		[[Category: Mcsg]]
Line 41:		Line 41:
	[[Category: Unknown function]]		[[Category: Unknown function]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Feb 17 13~~:29:~~54 2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 15:56:38 2010''