2g5l - Revision history

OCA at 08:47, 25 October 2023

2023-10-25T08:47:53Z

←Older revision		Revision as of 08:47, 25 October 2023
Line 3:		Line 3:
	<StructureSection load='2g5l' size='340' side='right'caption='[[2g5l]], [[Resolution\|resolution]] 1.15Å' scene=''>		<StructureSection load='2g5l' size='340' side='right'caption='[[2g5l]], [[Resolution\|resolution]] 1.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2g5l]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~As_4.1583 As 4.1583~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G5L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2g5l]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G5L FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.15Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5l OCA], [https://pdbe.org/2g5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g5l RCSB], [https://www.ebi.ac.uk/pdbsum/2g5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g5l ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5l OCA], [https://pdbe.org/2g5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g5l RCSB], [https://www.ebi.ac.uk/pdbsum/2g5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g5l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g5l ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g5l ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A novel peptide was designed which possesses nanomolar affinity of less than 20 nM for streptavidin. Therefore it was termed Nano-tag and has been used as an affinity tag for recombinant proteins. The minimized version of the wild type Nano-tag is a seven-amino acid peptide with the sequence fMDVEAWL. The three-dimensional structure of wild type streptavidin in complex with the minimized Nano-tag was analyzed at atomic resolution of 1.15 A and the details of the binding motif were investigated. The peptide recognizes the same pocket of streptavidin where the natural ligand biotin is bound, but the peptide requires significantly more space than biotin. Therefore the binding loop adopts an "open" conformation in order to release additional space for the peptide. The conformation of the bound Nano-tag corresponds to a 3(10) helix. However, the analysis of the intermolecular interactions of the Nano-tag with residues of the binding pocket of streptavidin reveals astonishing similarities to the biotin binding motif. In principle the three-dimensional conformation of the Nano-tag mimics the binding mode of biotin. Our results explain why the use of the Nano-tag in fusion with recombinant proteins is restricted to their N-terminus and we describe the special significance of the fMet residue for the high affinity binding mode.
		+
		+	High resolution structure of streptavidin in complex with a novel high affinity peptide tag mimicking the biotin binding motif.,Perbandt M, Bruns O, Vallazza M, Lamla T, Betzel Ch, Erdmann VA Proteins. 2007 Jun 1;67(4):1147-53. doi: 10.1002/prot.21236. PMID:17377987<ref>PMID:17377987</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 2g5l" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Avidin 3D structures\|Avidin 3D structures]]		*[[Avidin 3D structures\|Avidin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Betzel, C]]	+	[[Category: Streptomyces avidinii]]
-	[[Category: Bruns, O]]	+	[[Category: Betzel C]]
-	[[Category: Erdmann~~, V A~~]]	+	[[Category: Bruns O]]
-	[[Category: Lamla, T]]	+	[[Category: Erdmann VA]]
-	[[Category: Perbandt, M]]	+	[[Category: Lamla T]]
-	[[Category: Vallazza, M]]	+	[[Category: Perbandt M]]
-	~~[[Category: Binding peptid]]~~	+	[[Category: Vallazza M]]
-	~~[[Category: Biotin]]~~	+
-	~~[[Category: Nanotag]]~~	+
-	~~[[Category: Peptide binding protein]]~~	+
-	~~[[Category: Streptavidin~~]]	+

OCA at 14:40, 29 December 2021

2021-12-29T14:40:54Z

←Older revision		Revision as of 14:40, 29 December 2021
Line 3:		Line 3:
	<StructureSection load='2g5l' size='340' side='right'caption='[[2g5l]], [[Resolution\|resolution]] 1.15Å' scene=''>		<StructureSection load='2g5l' size='340' side='right'caption='[[2g5l]], [[Resolution\|resolution]] 1.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2g5l]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2G5L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2g5l]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G5L FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2g5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5l OCA], [~~http~~://pdbe.org/2g5l PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2g5l RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2g5l PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2g5l ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5l OCA], [https://pdbe.org/2g5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g5l RCSB], [https://www.ebi.ac.uk/pdbsum/2g5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g5l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:09, 27 March 2020

2020-03-27T11:09:58Z

←Older revision		Revision as of 11:09, 27 March 2020
Line 1:		Line 1:

	==Streptavidin in complex with Nanotag==		==Streptavidin in complex with Nanotag==
-	<StructureSection load='2g5l' size='340' side='right' caption='[[2g5l]], [[Resolution\|resolution]] 1.15Å' scene=''>	+	<StructureSection load='2g5l' size='340' side='right'caption='[[2g5l]], [[Resolution\|resolution]] 1.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2g5l]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G5L FirstGlance]. <br>		<table><tr><td colspan='2'>[[2g5l]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G5L FirstGlance]. <br>
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Avidin\|Avidin]]	+	*[[Avidin 3D structures\|Avidin 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: As 4 1583]]		[[Category: As 4 1583]]
		+	[[Category: Large Structures]]
	[[Category: Betzel, C]]		[[Category: Betzel, C]]
	[[Category: Bruns, O]]		[[Category: Bruns, O]]

OCA at 07:15, 20 June 2018

2018-06-20T07:15:22Z

←Older revision		Revision as of 07:15, 20 June 2018
Line 1:		Line 1:
		+
	==Streptavidin in complex with Nanotag==		==Streptavidin in complex with Nanotag==
	<StructureSection load='2g5l' size='340' side='right' caption='[[2g5l]], [[Resolution\|resolution]] 1.15Å' scene=''>		<StructureSection load='2g5l' size='340' side='right' caption='[[2g5l]], [[Resolution\|resolution]] 1.15Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5l OCA], [http://pdbe.org/2g5l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g5l RCSB], [http://www.ebi.ac.uk/pdbsum/2g5l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5l OCA], [http://pdbe.org/2g5l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g5l RCSB], [http://www.ebi.ac.uk/pdbsum/2g5l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2g5l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/2g5l_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/2g5l_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 03:10, 7 February 2016

2016-02-07T03:10:00Z

←Older revision		Revision as of 03:10, 7 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g5l ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>

OCA at 21:06, 9 September 2015

2015-09-09T21:06:16Z

←Older revision		Revision as of 21:06, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='2g5l' size='340' side='right' caption='[[2g5l]], [[Resolution\|resolution]] 1.15Å' scene=''>		<StructureSection load='2g5l' size='340' side='right' caption='[[2g5l]], [[Resolution\|resolution]] 1.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2g5l]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_avidinii Streptomyces avidinii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G5L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2g5l]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G5L FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g5l RCSB], [http://www.ebi.ac.uk/pdbsum/2g5l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5l OCA], [http://pdbe.org/2g5l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2g5l RCSB], [http://www.ebi.ac.uk/pdbsum/2g5l PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces avidinii~~]]	+	[[Category: As 4 1583]]
	[[Category: Betzel, C]]		[[Category: Betzel, C]]
	[[Category: Bruns, O]]		[[Category: Bruns, O]]

OCA at 07:22, 24 December 2014

2014-12-24T07:22:52Z

←Older revision		Revision as of 07:22, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2g5l]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G5L FirstGlance]. <br>		<table><tr><td colspan='2'>[[2g5l]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G5L FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g5l RCSB], [http://www.ebi.ac.uk/pdbsum/2g5l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g5l RCSB], [http://www.ebi.ac.uk/pdbsum/2g5l PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 23:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
-	[[Category: Betzel, C.]]	+	[[Category: Betzel, C]]
-	[[Category: Bruns, O.]]	+	[[Category: Bruns, O]]
-	[[Category: Erdmann, V A.]]	+	[[Category: Erdmann, V A]]
-	[[Category: Lamla, T.]]	+	[[Category: Lamla, T]]
-	[[Category: Perbandt, M.]]	+	[[Category: Perbandt, M]]
-	[[Category: Vallazza, M.]]	+	[[Category: Vallazza, M]]
	[[Category: Binding peptid]]		[[Category: Binding peptid]]
	[[Category: Biotin]]		[[Category: Biotin]]

OCA at 02:32, 29 September 2014

2014-09-29T02:32:00Z

←Older revision		Revision as of 02:32, 29 September 2014
Line 1:		Line 1:
-	~~[[Image:2g5l.png\|left\|200px]]~~	+	==Streptavidin in complex with Nanotag==
-		+	<StructureSection load='2g5l' size='340' side='right' caption='[[2g5l]], [[Resolution\|resolution]] 1.15Å' scene=''>
-	~~{{STRUCTURE_2g5l\| PDB=2g5l \| SCENE= }}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[2g5l]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2G5L FirstGlance]. <br>
-	===Streptavidin in complex with Nanotag===	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
-		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
-		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2g5l RCSB], [http://www.ebi.ac.uk/pdbsum/2g5l PDBsum]</span></td></tr>
-	==~~About this Structure~~==	+	<table>
-	[[2g5l]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA].	+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/2g5l_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>

	==See Also==		==See Also==
	*[[Avidin\|Avidin]]		*[[Avidin\|Avidin]]
		+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
	[[Category: Betzel, C.]]		[[Category: Betzel, C.]]

OCA at 05:25, 20 October 2012

2012-10-20T05:25:54Z

←Older revision		Revision as of 05:25, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[2g5l]] is a 4 chain structure ~~of [[Avidin]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA].	+	[[2g5l]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5L OCA].

	==See Also==		==See Also==

OCA at 10:00, 25 July 2012

2012-07-25T10:00:44Z

←Older revision		Revision as of 10:00, 25 July 2012
Line 1:		Line 1:
	[[Image:2g5l.png\|left\|200px]]		[[Image:2g5l.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2g5l", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2g5l\| PDB=2g5l \| SCENE= }}		{{STRUCTURE_2g5l\| PDB=2g5l \| SCENE= }}

Line 16:		Line 10:

	==See Also==		==See Also==
-	*[[Avidin]]	+	*[[Avidin\|Avidin]]
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
	[[Category: Betzel, C.]]		[[Category: Betzel, C.]]