2ha6 - Revision history

OCA at 08:50, 25 October 2023

2023-10-25T08:50:36Z

←Older revision		Revision as of 08:50, 25 October 2023
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	<StructureSection load='2ha6' size='340' side='right'caption='[[2ha6]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2ha6' size='340' side='right'caption='[[2ha6]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ha6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HA6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ha6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HA6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SCK:2,2-[(1,4-DIOXOBUTANE-1,4-DIYL)BIS(OXY)]BIS(N,N,N-TRIMETHYLETHANAMINIUM)'>SCK</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.25Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1j06\|1j06]], [[2h9y\|2h9y]], [[2ha0\|2ha0]], [[2ha2\|2ha2]], [[2ha3\|2ha3]], [[2ha4\|2ha4]], [[2ha5\|2ha5]], [[2ha7\|2ha7]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SCK:2,2-[(1,4-DIOXOBUTANE-1,4-DIYL)BIS(OXY)]BIS(N,N,N-TRIMETHYLETHANAMINIUM)'>SCK</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ha6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ha6 OCA], [https://pdbe.org/2ha6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ha6 RCSB], [https://www.ebi.ac.uk/pdbsum/2ha6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ha6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ha6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ha6 OCA], [https://pdbe.org/2ha6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ha6 RCSB], [https://www.ebi.ac.uk/pdbsum/2ha6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ha6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ACES_MOUSE ACES_MOUSE]] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.	+	[https://www.uniprot.org/uniprot/ACES_MOUSE ACES_MOUSE] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Acetylcholinesterase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Bourne, Y]]	+	[[Category: Bourne Y]]
-	[[Category: Kim, E]]	+	[[Category: Kim E]]
-	[[Category: Marchot, P]]	+	[[Category: Marchot P]]
-	[[Category: Radic, Z]]	+	[[Category: Radic Z]]
-	[[Category: Sulzenbacher, G]]	+	[[Category: Sulzenbacher G]]
-	[[Category: Taylor, P]]	+	[[Category: Taylor P]]
-	~~[[Category: Glycosylated protein]]~~	+
-	~~[[Category: Homodimer]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase fold]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: Serine esterase~~]]	+

OCA at 15:14, 2 June 2021

2021-06-02T15:14:03Z

←Older revision		Revision as of 15:14, 2 June 2021
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		+
	==Crystal structure of mutant S203A of mouse acetylcholinesterase complexed with succinylcholine==		==Crystal structure of mutant S203A of mouse acetylcholinesterase complexed with succinylcholine==
-	<StructureSection load='2ha6' size='340' side='right' caption='[[2ha6]], [[Resolution\|resolution]] 2.25Å' scene=''>	+	<StructureSection load='2ha6' size='340' side='right'caption='[[2ha6]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ha6]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HA6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ha6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HA6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SCK:2,2-[(1,4-DIOXOBUTANE-1,4-DIYL)BIS(OXY)]BIS(N,N,N-TRIMETHYLETHANAMINIUM)'>SCK</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SCK:2,2-[(1,4-DIOXOBUTANE-1,4-DIYL)BIS(OXY)]BIS(N,N,N-TRIMETHYLETHANAMINIUM)'>SCK</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j06\|1j06]], [[2h9y\|2h9y]], [[2ha0\|2ha0]], [[2ha2\|2ha2]], [[2ha3\|2ha3]], [[2ha4\|2ha4]], [[2ha5\|2ha5]], [[2ha7\|2ha7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1j06\|1j06]], [[2h9y\|2h9y]], [[2ha0\|2ha0]], [[2ha2\|2ha2]], [[2ha3\|2ha3]], [[2ha4\|2ha4]], [[2ha5\|2ha5]], [[2ha7\|2ha7]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ha6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ha6 OCA], [~~http~~://pdbe.org/2ha6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ha6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ha6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ha6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ha6 OCA], [https://pdbe.org/2ha6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ha6 RCSB], [https://www.ebi.ac.uk/pdbsum/2ha6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ha6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ACES_MOUSE ACES_MOUSE]] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.	+	[[https://www.uniprot.org/uniprot/ACES_MOUSE ACES_MOUSE]] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ha/2ha6_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ha/2ha6_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 32:

	==See Also==		==See Also==
-	*[[3D structures ~~of acetylcholinesterase~~\|3D structures ~~of acetylcholinesterase~~]]	+	*[[Acetylcholinesterase 3D structures\|Acetylcholinesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Acetylcholinesterase]]		[[Category: Acetylcholinesterase]]
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Bourne, Y]]		[[Category: Bourne, Y]]

OCA at 12:27, 8 February 2016

2016-02-08T12:27:08Z

←Older revision		Revision as of 12:27, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ha6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[~~Acetylcholinesterase~~\|~~Acetylcholinesterase~~]]	+	*[[3D structures of acetylcholinesterase\|3D structures of acetylcholinesterase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:34, 10 September 2015

2015-09-10T11:34:09Z

←Older revision		Revision as of 11:34, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2ha6' size='340' side='right' caption='[[2ha6]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2ha6' size='340' side='right' caption='[[2ha6]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ha6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HA6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ha6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HA6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SCK:2,2-[(1,4-DIOXOBUTANE-1,4-DIYL)BIS(OXY)]BIS(N,N,N-TRIMETHYLETHANAMINIUM)'>SCK</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SCK:2,2-[(1,4-DIOXOBUTANE-1,4-DIYL)BIS(OXY)]BIS(N,N,N-TRIMETHYLETHANAMINIUM)'>SCK</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j06\|1j06]], [[2h9y\|2h9y]], [[2ha0\|2ha0]], [[2ha2\|2ha2]], [[2ha3\|2ha3]], [[2ha4\|2ha4]], [[2ha5\|2ha5]], [[2ha7\|2ha7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j06\|1j06]], [[2h9y\|2h9y]], [[2ha0\|2ha0]], [[2ha2\|2ha2]], [[2ha3\|2ha3]], [[2ha4\|2ha4]], [[2ha5\|2ha5]], [[2ha7\|2ha7]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ha6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ha6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ha6 RCSB], [http://www.ebi.ac.uk/pdbsum/2ha6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ha6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ha6 OCA], [http://pdbe.org/2ha6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ha6 RCSB], [http://www.ebi.ac.uk/pdbsum/2ha6 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ha6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Acetylcholinesterase]]		[[Category: Acetylcholinesterase]]
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Bourne, Y]]		[[Category: Bourne, Y]]
	[[Category: Kim, E]]		[[Category: Kim, E]]

OCA at 23:18, 24 December 2014

2014-12-24T23:18:55Z

←Older revision		Revision as of 23:18, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ha6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HA6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ha6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HA6 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SCK:2,2-[(1,4-DIOXOBUTANE-1,4-DIYL)BIS(OXY)]BIS(N,N,N-TRIMETHYLETHANAMINIUM)'>SCK</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SCK:2,2-[(1,4-DIOXOBUTANE-1,4-DIYL)BIS(OXY)]BIS(N,N,N-TRIMETHYLETHANAMINIUM)'>SCK</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j06\|1j06]], [[2h9y\|2h9y]], [[2ha0\|2ha0]], [[2ha2\|2ha2]], [[2ha3\|2ha3]], [[2ha4\|2ha4]], [[2ha5\|2ha5]], [[2ha7\|2ha7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j06\|1j06]], [[2h9y\|2h9y]], [[2ha0\|2ha0]], [[2ha2\|2ha2]], [[2ha3\|2ha3]], [[2ha4\|2ha4]], [[2ha5\|2ha5]], [[2ha7\|2ha7]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ha6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ha6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ha6 RCSB], [http://www.ebi.ac.uk/pdbsum/2ha6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ha6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ha6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ha6 RCSB], [http://www.ebi.ac.uk/pdbsum/2ha6 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACES_MOUSE ACES_MOUSE]] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Acetylcholinesterase]]		[[Category: Acetylcholinesterase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Bourne, Y.]]	+	[[Category: Bourne, Y]]
-	[[Category: Kim, E.]]	+	[[Category: Kim, E]]
-	[[Category: Marchot, P.]]	+	[[Category: Marchot, P]]
-	[[Category: Radic, Z.]]	+	[[Category: Radic, Z]]
-	[[Category: Sulzenbacher, G.]]	+	[[Category: Sulzenbacher, G]]
-	[[Category: Taylor, P~~.]]~~	+	[[Category: Taylor, P]]
-	~~[[Category: Acetylcholinesterase~~]]	+
	[[Category: Glycosylated protein]]		[[Category: Glycosylated protein]]
	[[Category: Homodimer]]		[[Category: Homodimer]]

OCA at 03:19, 29 September 2014

2014-09-29T03:19:41Z

←Older revision		Revision as of 03:19, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2ha6.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of mutant S203A of mouse acetylcholinesterase complexed with succinylcholine==
		+	<StructureSection load='2ha6' size='340' side='right' caption='[[2ha6]], [[Resolution\|resolution]] 2.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ha6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HA6 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SCK:2,2-[(1,4-DIOXOBUTANE-1,4-DIYL)BIS(OXY)]BIS(N,N,N-TRIMETHYLETHANAMINIUM)'>SCK</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j06\|1j06]], [[2h9y\|2h9y]], [[2ha0\|2ha0]], [[2ha2\|2ha2]], [[2ha3\|2ha3]], [[2ha4\|2ha4]], [[2ha5\|2ha5]], [[2ha7\|2ha7]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ha6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ha6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ha6 RCSB], [http://www.ebi.ac.uk/pdbsum/2ha6 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ha/2ha6_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hydrolysis of acetylcholine catalyzed by acetylcholinesterase (AChE), one of the most efficient enzymes in nature, occurs at the base of a deep and narrow active center gorge. At the entrance of the gorge, the peripheral anionic site provides a binding locus for allosteric ligands, including substrates. To date, no structural information on substrate entry to the active center from the peripheral site of AChE or its subsequent egress has been reported. Complementary crystal structures of mouse AChE and an inactive mouse AChE mutant with a substituted catalytic serine (S203A), in various complexes with four substrates (acetylcholine, acetylthiocholine, succinyldicholine, and butyrylthiocholine), two non-hydrolyzable substrate analogues (m-(N,N,N-trimethylammonio)-trifluoroacetophenone and 4-ketoamyltrimethylammonium), and one reaction product (choline) were solved in the 2.05-2.65-A resolution range. These structures, supported by binding and inhibition data obtained on the same complexes, reveal the successive positions and orientations of the substrates bound to the peripheral site and proceeding within the gorge toward the active site, the conformations of the presumed transition state for acylation and the acyl-enzyme intermediate, and the positions and orientations of the dissociating and egressing products. Moreover, the structures of the AChE mutant in complexes with acetylthiocholine and succinyldicholine reveal additional substrate binding sites on the enzyme surface, distal to the gorge entry. Hence, we provide a comprehensive set of structural snapshots of the steps leading to the intermediates of catalysis and the potential regulation by substrate binding to various allosteric sites at the enzyme surface.

-	~~{{STRUCTURE_2ha6\| PDB=2ha6 \| SCENE= }}~~	+	Substrate and product trafficking through the active center gorge of acetylcholinesterase analyzed by crystallography and equilibrium binding.,Bourne Y, Radic Z, Sulzenbacher G, Kim E, Taylor P, Marchot P J Biol Chem. 2006 Sep 29;281(39):29256-67. Epub 2006 Jul 12. PMID:16837465<ref>PMID:16837465</ref>

-	~~===Crystal structure~~ of ~~mutant S203A of mouse acetylcholinesterase complexed with succinylcholine===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16837465}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2ha6]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA].~~	+

	==See Also==		==See Also==
	*[[Acetylcholinesterase\|Acetylcholinesterase]]		*[[Acetylcholinesterase\|Acetylcholinesterase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016837465<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Acetylcholinesterase]]		[[Category: Acetylcholinesterase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 10:08, 20 October 2012

2012-10-20T10:08:07Z

←Older revision		Revision as of 10:08, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[2ha6]] is a 2 chain structure ~~of [[Acetylcholinesterase]]~~ with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA].	+	[[2ha6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA].

	==See Also==		==See Also==

OCA at 01:05, 26 July 2012

2012-07-26T01:05:41Z

←Older revision		Revision as of 01:05, 26 July 2012
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	[[Image:2ha6.png\|left\|200px]]		[[Image:2ha6.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2ha6", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2ha6\| PDB=2ha6 \| SCENE= }}		{{STRUCTURE_2ha6\| PDB=2ha6 \| SCENE= }}

	===Crystal structure of mutant S203A of mouse acetylcholinesterase complexed with succinylcholine===		===Crystal structure of mutant S203A of mouse acetylcholinesterase complexed with succinylcholine===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16837465}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16837465 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_16837465}}		{{ABSTRACT_PUBMED_16837465}}

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	==See Also==		==See Also==
-	*[[Acetylcholinesterase]]	+	*[[Acetylcholinesterase\|Acetylcholinesterase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16837465~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016837465</ref><references group="xtra"/>
	[[Category: Acetylcholinesterase]]		[[Category: Acetylcholinesterase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
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	[[Category: Glycosylated protein]]		[[Category: Glycosylated protein]]
	[[Category: Homodimer]]		[[Category: Homodimer]]
		+	[[Category: Hydrolase]]
	[[Category: Hydrolase fold]]		[[Category: Hydrolase fold]]
	[[Category: Mutant]]		[[Category: Mutant]]
	[[Category: Serine esterase]]		[[Category: Serine esterase]]

OCA: Protected "2ha6" [edit=sysop:move=sysop]

2010-12-26T20:55:02Z

Protected "2ha6" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:55, 26 December 2010

OCA at 20:55, 26 December 2010

2010-12-26T20:55:01Z

←Older revision		Revision as of 20:55, 26 December 2010
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-	{{Seed}}
	[[Image:2ha6.png\|left\|200px]]		[[Image:2ha6.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~2HA6~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA].	+	[[2ha6]] is a 2 chain structure of [[Acetylcholinesterase]] with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HA6 OCA].
		+
		+	==See Also==
		+	*[[Acetylcholinesterase]]

	==Reference==		==Reference==
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	[[Category: Mutant]]		[[Category: Mutant]]
	[[Category: Serine esterase]]		[[Category: Serine esterase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:59:59 2009''