2him - Revision history

OCA at 07:41, 17 March 2021

2021-03-17T07:41:01Z

←Older revision		Revision as of 07:41, 17 March 2021
Line 1:		Line 1:

	==Crystal Structure and Allosteric Regulation of the Cytoplasmic Escherichia coli L-Asparaginase I==		==Crystal Structure and Allosteric Regulation of the Cytoplasmic Escherichia coli L-Asparaginase I==
-	<StructureSection load='2him' size='340' side='right' caption='[[2him]], [[Resolution\|resolution]] 1.82Å' scene=''>	+	<StructureSection load='2him' size='340' side='right'caption='[[2him]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2him]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HIM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2him]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HIM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASN:ASPARAGINE'>ASN</scene>, <scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASN:ASPARAGINE'>ASN</scene>, <scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2p2d\|2p2d]], [[2p2n\|2p2n]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2p2d\|2p2d]], [[2p2n\|2p2n]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ansA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ansA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2him OCA], [~~http~~://pdbe.org/2him PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2him RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2him PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2him ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2him OCA], [https://pdbe.org/2him PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2him RCSB], [https://www.ebi.ac.uk/pdbsum/2him PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2him ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Asparaginase\|Asparaginase]]	+	*[[Asparaginase 3D structures\|Asparaginase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
		+	[[Category: Large Structures]]
	[[Category: Heath, R J]]		[[Category: Heath, R J]]
	[[Category: Nourse, A]]		[[Category: Nourse, A]]

OCA at 06:50, 27 June 2018

2018-06-27T06:50:51Z

←Older revision		Revision as of 06:50, 27 June 2018
Line 1:		Line 1:
		+
	==Crystal Structure and Allosteric Regulation of the Cytoplasmic Escherichia coli L-Asparaginase I==		==Crystal Structure and Allosteric Regulation of the Cytoplasmic Escherichia coli L-Asparaginase I==
	<StructureSection load='2him' size='340' side='right' caption='[[2him]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='2him' size='340' side='right' caption='[[2him]], [[Resolution\|resolution]] 1.82Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ansA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ansA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2him OCA], [http://pdbe.org/2him PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2him RCSB], [http://www.ebi.ac.uk/pdbsum/2him PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2him OCA], [http://pdbe.org/2him PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2him RCSB], [http://www.ebi.ac.uk/pdbsum/2him PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2him ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/2him_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/2him_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2him ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:31, 11 September 2015

2015-09-11T09:31:51Z

←Older revision		Revision as of 09:31, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2him' size='340' side='right' caption='[[2him]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='2him' size='340' side='right' caption='[[2him]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2him]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HIM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2him]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HIM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASN:ASPARAGINE'>ASN</scene>, <scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASN:ASPARAGINE'>ASN</scene>, <scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2p2d\|2p2d]], [[2p2n\|2p2n]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2p2d\|2p2d]], [[2p2n\|2p2n]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ansA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ansA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2him OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2him RCSB], [http://www.ebi.ac.uk/pdbsum/2him PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2him OCA], [http://pdbe.org/2him PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2him RCSB], [http://www.ebi.ac.uk/pdbsum/2him PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2him" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
-	[[Category: Escherichia coli]]
	[[Category: Heath, R J]]		[[Category: Heath, R J]]
	[[Category: Nourse, A]]		[[Category: Nourse, A]]

OCA at 08:39, 16 January 2015

2015-01-16T08:39:19Z

←Older revision		Revision as of 08:39, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2him]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HIM FirstGlance]. <br>		<table><tr><td colspan='2'>[[2him]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HIM FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASN:ASPARAGINE'>ASN</scene>, <scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASN:ASPARAGINE'>ASN</scene>, <scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2p2d\|2p2d]], [[2p2n\|2p2n]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2p2d\|2p2d]], [[2p2n\|2p2n]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ansA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ansA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2him OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2him RCSB], [http://www.ebi.ac.uk/pdbsum/2him PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2him OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2him RCSB], [http://www.ebi.ac.uk/pdbsum/2him PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Heath, R J.]]	+	[[Category: Heath, R J]]
-	[[Category: Nourse, A.]]	+	[[Category: Nourse, A]]
-	[[Category: Rock, C O.]]	+	[[Category: Rock, C O]]
-	[[Category: White, S W.]]	+	[[Category: White, S W]]
-	[[Category: Yun, M K~~.]]~~	+	[[Category: Yun, M K]]
-	~~[[Category: Asparaginase~~]]	+
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 10:13, 30 September 2014

2014-09-30T10:13:19Z

←Older revision		Revision as of 10:13, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2him.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure and Allosteric Regulation of the Cytoplasmic Escherichia coli L-Asparaginase I==
		+	<StructureSection load='2him' size='340' side='right' caption='[[2him]], [[Resolution\|resolution]] 1.82Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2him]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HIM FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASN:ASPARAGINE'>ASN</scene>, <scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2p2d\|2p2d]], [[2p2n\|2p2n]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ansA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2him OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2him RCSB], [http://www.ebi.ac.uk/pdbsum/2him PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/2him_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Analytical ultracentifugation and X-ray crystallography reveal that AnsA forms a tetrameric structure as a dimer of two intimate dimers. Kinetic analysis of the enzyme reveals that AnsA is positively cooperative, displaying a sigmoidal substrate dependence curve with an [S](0.5) of 1 mM L-asparagine and a Hill coefficient (n(H)) of 2.6. Binding of L-asparagine to an allosteric site was observed in the crystal structure concomitant with a reorganization of the quarternary structure, relative to the apo enzyme. The carboxyl group of the bound asparagine makes salt bridges and hydrogen bonds to Arg240, while the N(delta2) nitrogen interacts with Thr162. Mutation of Arg240 to Ala increases the [S](0.5) value to 5.9 mM, presumably by reducing the affinity of the site for L-asparagine, although the enzyme retains cooperativity. Mutation of Thr162 to Ala results in an active enzyme with no cooperativity. Transmission of the signal from the allosteric site to the active site appears to involve subtle interactions at the dimer-dimer interface and relocation of Gln118 into the vicinity of the active site to position the probable catalytic water molecule. These data define the structural basis for the cooperative regulation of the intracellular asparaginase that is required for proper functioning within the cell.

-	~~{{STRUCTURE_2him\| PDB=2him \| SCENE= }}~~	+	Crystal structure and allosteric regulation of the cytoplasmic Escherichia coli L-asparaginase I.,Yun MK, Nourse A, White SW, Rock CO, Heath RJ J Mol Biol. 2007 Jun 8;369(3):794-811. Epub 2007 Mar 30. PMID:17451745<ref>PMID:17451745</ref>

-	~~===Crystal Structure and Allosteric Regulation~~ of the ~~Cytoplasmic Escherichia coli L-Asparaginase I===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17451745}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2him]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2HIM OCA].~~	+

	==See Also==		==See Also==
	*[[Asparaginase\|Asparaginase]]		*[[Asparaginase\|Asparaginase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:017451745<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA: Protected "2him" [edit=sysop:move=sysop]

2013-01-07T14:41:21Z

Protected "2him" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:41, 7 January 2013

OCA at 14:41, 7 January 2013

2013-01-07T14:41:19Z

←Older revision		Revision as of 14:41, 7 January 2013
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-	{{Seed}}
	[[Image:2him.png\|left\|200px]]		[[Image:2him.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2him", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_2him\| PDB=2him \| SCENE= }}		{{STRUCTURE_2him\| PDB=2him \| SCENE= }}

	===Crystal Structure and Allosteric Regulation of the Cytoplasmic Escherichia coli L-Asparaginase I===		===Crystal Structure and Allosteric Regulation of the Cytoplasmic Escherichia coli L-Asparaginase I===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17451745}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17451745 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17451745}}		{{ABSTRACT_PUBMED_17451745}}

	==About this Structure==		==About this Structure==
-	~~2HIM~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIM OCA].	+	[[2him]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIM OCA].
		+
		+	==See Also==
		+	*[[Asparaginase\|Asparaginase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17451745~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017451745</ref><references group="xtra"/>
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
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	[[Category: Asparaginase]]		[[Category: Asparaginase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 19 08:41:20 2009''

OCA at 05:41, 19 April 2009

2009-04-19T05:41:20Z

←Older revision		Revision as of 05:41, 19 April 2009
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	[[Category: Yun, M K.]]		[[Category: Yun, M K.]]
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
		+	[[Category: Hydrolase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Feb 17 18~~:47:50 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 19 08:41:20 2009''

OCA at 16:47, 17 February 2009

2009-02-17T16:47:52Z

←Older revision		Revision as of 16:47, 17 February 2009
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	==About this Structure==		==About this Structure==
-	2HIM is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIM OCA].	+	2HIM is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIM OCA].

	==Reference==		==Reference==
-	Crystal structure and allosteric regulation of the cytoplasmic Escherichia coli L-asparaginase I., Yun MK, Nourse A, White SW, Rock CO, Heath RJ, J Mol Biol. 2007 Jun 8;369(3):794-811. Epub 2007 Mar 30. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17451745 17451745]~~	+	<ref group="xtra">PMID:17451745</ref><references group="xtra"/>
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Heath, R J.]]		[[Category: Heath, R J.]]
	[[Category: Nourse, A.]]		[[Category: Nourse, A.]]
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	[[Category: Asparaginase]]		[[Category: Asparaginase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 02~~:50:~~52 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:47:50 2009''

OCA at 23:50, 28 July 2008

2008-07-28T23:50:52Z

←Older revision		Revision as of 23:50, 28 July 2008
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-	[[Image:2him.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2him.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2him\| PDB=2him \| SCENE= }}		{{STRUCTURE_2him\| PDB=2him \| SCENE= }}

-	~~'''~~Crystal Structure and Allosteric Regulation of the Cytoplasmic Escherichia coli L-Asparaginase I~~'''~~	+	===Crystal Structure and Allosteric Regulation of the Cytoplasmic Escherichia coli L-Asparaginase I===


-	~~==Overview==~~	+	<!--
-	~~AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Analytical ultracentifugation and X~~-ray crystallography reveal that AnsA forms a tetrameric structure as a dimer of two intimate dimers. Kinetic analysis of the enzyme reveals that AnsA is positively cooperative, displaying a sigmoidal substrate dependence curve with an [S](0.5) of 1 mM L-asparagine and a Hill coefficient (n(H)) of 2.6. Binding of L-asparagine to an allosteric site was observed in the crystal structure concomitant with a reorganization of the quarternary structure, relative to the apo enzyme. The ~~carboxyl group of the bound asparagine makes salt bridges and hydrogen bonds to Arg240~~, ~~while~~ the ~~N(delta2) nitrogen interacts with Thr162. Mutation of Arg240~~ to ~~Ala increases~~ the ~~[S]~~(0.5) ~~value to 5.9 mM~~, ~~presumably by reducing~~ the ~~affinity of the site for L-asparagine, although the enzyme retains cooperativity~~. ~~Mutation of Thr162 to Ala results in an active enzyme with no cooperativity. Transmission of the signal from the allosteric site to the active site appears to involve subtle interactions at the dimer~~-dimer interface and relocation of Gln118 into the vicinity of the active site to position the probable catalytic water molecule. These data define the structural basis for the cooperative regulation of the intracellular asparaginase that is required for proper functioning within the cell.	+	The line below this paragraph, {{ABSTRACT_PUBMED_17451745}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17451745 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_17451745}}

	==About this Structure==		==About this Structure==
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	[[Category: Yun, M K.]]		[[Category: Yun, M K.]]
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
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