2hj3 - Revision history

OCA at 11:09, 5 January 2022

2022-01-05T11:09:25Z

←Older revision		Revision as of 11:09, 5 January 2022
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	<StructureSection load='2hj3' size='340' side='right'caption='[[2hj3]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2hj3' size='340' side='right'caption='[[2hj3]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hj3]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HJ3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hj3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HJ3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jr8\|1jr8]], [[1jra\|1jra]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1jr8\|1jr8]], [[1jra\|1jra]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2hj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hj3 OCA], [~~http~~://pdbe.org/2hj3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2hj3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2hj3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2hj3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hj3 OCA], [https://pdbe.org/2hj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hj3 RCSB], [https://www.ebi.ac.uk/pdbsum/2hj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hj3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ERV1_ARATH ERV1_ARATH]] FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Oxidizes thioredoxin in vitro.[PROSITE-ProRule:PRU00654]<ref>PMID:14996837</ref>	+	[[https://www.uniprot.org/uniprot/ERV1_ARATH ERV1_ARATH]] FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Oxidizes thioredoxin in vitro.[PROSITE-ProRule:PRU00654]<ref>PMID:14996837</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:10, 1 April 2020

2020-04-01T10:10:51Z

←Older revision		Revision as of 10:10, 1 April 2020
Line 1:		Line 1:

	==Structure of the Arabidopsis Thaliana Erv1 Thiol Oxidase==		==Structure of the Arabidopsis Thaliana Erv1 Thiol Oxidase==
-	<StructureSection load='2hj3' size='340' side='right' caption='[[2hj3]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='2hj3' size='340' side='right'caption='[[2hj3]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hj3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJ3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hj3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJ3 FirstGlance]. <br>
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Sulfhydryl oxidase\|Sulfhydryl oxidase]]	+	*[[Sulfhydryl oxidase 3D structures\|Sulfhydryl oxidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Arath]]		[[Category: Arath]]
		+	[[Category: Large Structures]]
	[[Category: Fass, D]]		[[Category: Fass, D]]
	[[Category: Vitu, E]]		[[Category: Vitu, E]]

OCA at 06:09, 27 June 2018

2018-06-27T06:09:21Z

←Older revision		Revision as of 06:09, 27 June 2018
Line 1:		Line 1:
		+
	==Structure of the Arabidopsis Thaliana Erv1 Thiol Oxidase==		==Structure of the Arabidopsis Thaliana Erv1 Thiol Oxidase==
	<StructureSection load='2hj3' size='340' side='right' caption='[[2hj3]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2hj3' size='340' side='right' caption='[[2hj3]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jr8\|1jr8]], [[1jra\|1jra]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jr8\|1jr8]], [[1jra\|1jra]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hj3 OCA], [http://pdbe.org/2hj3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hj3 RCSB], [http://www.ebi.ac.uk/pdbsum/2hj3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hj3 OCA], [http://pdbe.org/2hj3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hj3 RCSB], [http://www.ebi.ac.uk/pdbsum/2hj3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hj3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/2hj3_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/2hj3_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:02, 7 February 2016

2016-02-07T20:02:21Z

←Older revision		Revision as of 20:02, 7 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hj3 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:34, 10 September 2015

2015-09-10T04:34:08Z

←Older revision		Revision as of 04:34, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2hj3' size='340' side='right' caption='[[2hj3]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2hj3' size='340' side='right' caption='[[2hj3]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hj3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Arabidopsis_thaliana Arabidopsis thaliana~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJ3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hj3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJ3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jr8\|1jr8]], [[1jra\|1jra]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jr8\|1jr8]], [[1jra\|1jra]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hj3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hj3 RCSB], [http://www.ebi.ac.uk/pdbsum/2hj3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hj3 OCA], [http://pdbe.org/2hj3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hj3 RCSB], [http://www.ebi.ac.uk/pdbsum/2hj3 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ERV1_ARATH ERV1_ARATH]] FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Oxidizes thioredoxin in vitro.[PROSITE-ProRule:PRU00654]<ref>PMID:14996837</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 25:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2hj3" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arabidopsis thaliana~~]]	+	[[Category: Arath]]
	[[Category: Fass, D]]		[[Category: Fass, D]]
	[[Category: Vitu, E]]		[[Category: Vitu, E]]

OCA at 08:47, 16 January 2015

2015-01-16T08:47:41Z

←Older revision		Revision as of 08:47, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hj3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJ3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hj3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJ3 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jr8\|1jr8]], [[1jra\|1jra]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jr8\|1jr8]], [[1jra\|1jra]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hj3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hj3 RCSB], [http://www.ebi.ac.uk/pdbsum/2hj3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hj3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hj3 RCSB], [http://www.ebi.ac.uk/pdbsum/2hj3 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
-	[[Category: Fass, D.]]	+	[[Category: Fass, D]]
-	[[Category: Vitu, E.]]	+	[[Category: Vitu, E]]
	[[Category: Flavin adenine dinucleotide]]		[[Category: Flavin adenine dinucleotide]]
	[[Category: Four-helix bundle]]		[[Category: Four-helix bundle]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 02:45, 29 September 2014

2014-09-29T02:45:18Z

←Older revision		Revision as of 02:45, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2hj3.~~png~~\|~~left~~\|200px]]	+	==Structure of the Arabidopsis Thaliana Erv1 Thiol Oxidase==
		+	<StructureSection load='2hj3' size='340' side='right' caption='[[2hj3]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2hj3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJ3 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jr8\|1jr8]], [[1jra\|1jra]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hj3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hj3 RCSB], [http://www.ebi.ac.uk/pdbsum/2hj3 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/2hj3_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ERV/ALR sulfhydryl oxidase domain is a versatile module adapted for catalysis of disulfide bond formation in various organelles and biological settings. Its four-helix bundle structure juxtaposes a Cys-X-X-Cys dithiol/disulfide motif with a bound flavin adenine dinucleotide (FAD) cofactor, enabling transfer of electrons from thiol substrates to non-thiol electron acceptors. ERV/ALR family members contain an additional di-cysteine motif outside the four-helix-bundle core. Although the location and context of this "shuttle" disulfide differs among family members, it is proposed to perform the same basic function of mediating electron transfer from substrate to the enzyme active site. We have determined by X-ray crystallography the structure of AtErv1, an ERV/ALR enzyme that contains a Cys-X4-Cys shuttle disulfide and oxidizes thioredoxin in vitro, and compared it to ScErv2, which has a Cys-X-Cys shuttle and does not oxidize thioredoxin at an appreciable rate. The AtErv1 shuttle disulfide is in a region of the structure that is disordered and thus apparently mobile and exposed. This feature may facilitate access of protein substrates to the shuttle disulfide. To test whether the shuttle disulfide region is modular and can confer on other enzymes oxidase activity toward new substrates, we generated chimeric enzyme variants combining shuttle disulfide and core elements from AtErv1 and ScErv2 and monitored oxidation of thioredoxin by the chimeras. We found that the AtErv1 shuttle disulfide region could indeed confer thioredoxin oxidase activity on the ScErv2 core. Remarkably, various chimeras containing the ScErv2 Cys-X-Cys shuttle disulfide were found to function efficiently as well. Since neither the ScErv2 core nor the Cys-X-Cys motif is therefore incapable of participating in oxidation of thioredoxin, we conclude that wild-type ScErv2 has evolved to repress activity on substrates of this type, perhaps in favor of a different, as yet unknown, substrate.

-	~~{{STRUCTURE_2hj3\| PDB=2hj3 \| SCENE= }}~~	+	Gain of function in an ERV/ALR sulfhydryl oxidase by molecular engineering of the shuttle disulfide.,Vitu E, Bentzur M, Lisowsky T, Kaiser CA, Fass D J Mol Biol. 2006 Sep 8;362(1):89-101. Epub 2006 Aug 8. PMID:16893552<ref>PMID:16893552</ref>

-	~~===Structure~~ of the ~~Arabidopsis Thaliana Erv1 Thiol Oxidase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16893552}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2hj3]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA].~~	+

	==See Also==		==See Also==
	*[[Sulfhydryl oxidase\|Sulfhydryl oxidase]]		*[[Sulfhydryl oxidase\|Sulfhydryl oxidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016893552<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Fass, D.]]		[[Category: Fass, D.]]

OCA at 07:43, 20 October 2012

2012-10-20T07:43:10Z

←Older revision		Revision as of 07:43, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[2hj3]] is a 2 chain structure ~~of [[Sulfhydryl oxidase]]~~ with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA].	+	[[2hj3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA].

	==See Also==		==See Also==

OCA: Protected "2hj3" [edit=sysop:move=sysop]

2012-07-25T17:14:03Z

Protected "2hj3" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:14, 25 July 2012

OCA at 17:14, 25 July 2012

2012-07-25T17:14:01Z

←Older revision		Revision as of 17:14, 25 July 2012
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-	{{Seed}}
	[[Image:2hj3.png\|left\|200px]]		[[Image:2hj3.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2hj3\| PDB=2hj3 \| SCENE= }}		{{STRUCTURE_2hj3\| PDB=2hj3 \| SCENE= }}

	===Structure of the Arabidopsis Thaliana Erv1 Thiol Oxidase===		===Structure of the Arabidopsis Thaliana Erv1 Thiol Oxidase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16893552}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16893552 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_16893552}}		{{ABSTRACT_PUBMED_16893552}}

	==About this Structure==		==About this Structure==
-	~~2HJ3~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA].	+	[[2hj3]] is a 2 chain structure of [[Sulfhydryl oxidase]] with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ3 OCA].
		+
		+	==See Also==
		+	*[[Sulfhydryl oxidase\|Sulfhydryl oxidase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16893552~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016893552</ref><references group="xtra"/>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Fass, D.]]		[[Category: Fass, D.]]
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	[[Category: Flavin adenine dinucleotide]]		[[Category: Flavin adenine dinucleotide]]
	[[Category: Four-helix bundle]]		[[Category: Four-helix bundle]]
-		+	[[Category: Oxidoreductase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 16:11:02 2009''~~	+