2hjr - Revision history

OCA at 09:58, 30 August 2023

2023-08-30T09:58:23Z

←Older revision		Revision as of 09:58, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='2hjr' size='340' side='right'caption='[[2hjr]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2hjr' size='340' side='right'caption='[[2hjr]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hjr]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Crypv Crypv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HJR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hjr]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HJR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hjr OCA], [https://pdbe.org/2hjr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hjr RCSB], [https://www.ebi.ac.uk/pdbsum/2hjr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hjr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hjr OCA], [https://pdbe.org/2hjr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hjr RCSB], [https://www.ebi.ac.uk/pdbsum/2hjr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hjr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5CYZ3_CRYPI Q5CYZ3_CRYPI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Crypv]]
-	[[Category: Large Structures]]
-	[[Category: Amani, M]]
-	[[Category: Arrowsmith, C H]]
-	[[Category: Bochkarev, A]]
-	[[Category: Dong, A]]
-	[[Category: Edwards, A M]]
-	[[Category: Hassani, A]]
-	[[Category: Hui, R]]
-	[[Category: Kozieradzki, I]]
-	[[Category: Lew, J]]
-	[[Category: Qiu, W]]
-	[[Category: Ren, H]]
-	[[Category: Structural genomic]]
-	[[Category: Sundstrom, M]]
-	[[Category: Weigelt, J]]
-	[[Category: Wernimont, A K]]
	[[Category: Cryptosporidium parvum]]		[[Category: Cryptosporidium parvum]]
-	[[Category: ~~Malaria~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Malate dehydrogenase~~]]	+	[[Category: Amani M]]
-	[[Category: ~~Oxidoreductase~~]]	+	[[Category: Arrowsmith CH]]
-	[[Category: ~~Sgc~~]]	+	[[Category: Bochkarev A]]
		+	[[Category: Dong A]]
		+	[[Category: Edwards AM]]
		+	[[Category: Hassani A]]
		+	[[Category: Hui R]]
		+	[[Category: Kozieradzki I]]
		+	[[Category: Lew J]]
		+	[[Category: Qiu W]]
		+	[[Category: Ren H]]
		+	[[Category: Sundstrom M]]
		+	[[Category: Weigelt J]]
		+	[[Category: Wernimont AK]]

OCA at 11:09, 5 January 2022

2022-01-05T11:09:37Z

←Older revision		Revision as of 11:09, 5 January 2022
Line 3:		Line 3:
	<StructureSection load='2hjr' size='340' side='right'caption='[[2hjr]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2hjr' size='340' side='right'caption='[[2hjr]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hjr]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Crypv Crypv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HJR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hjr]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Crypv Crypv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HJR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2hjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hjr OCA], [~~http~~://pdbe.org/2hjr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2hjr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2hjr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2hjr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hjr OCA], [https://pdbe.org/2hjr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hjr RCSB], [https://www.ebi.ac.uk/pdbsum/2hjr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hjr ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 10:11, 1 April 2020

2020-04-01T10:11:12Z

←Older revision		Revision as of 10:11, 1 April 2020
Line 1:		Line 1:

	==Crystal Structure of Cryptosporidium parvum malate dehydrogenase==		==Crystal Structure of Cryptosporidium parvum malate dehydrogenase==
-	<StructureSection load='2hjr' size='340' side='right' caption='[[2hjr]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='2hjr' size='340' side='right'caption='[[2hjr]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hjr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Crypv Crypv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJR FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hjr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Crypv Crypv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJR FirstGlance]. <br>
Line 28:		Line 28:

	==See Also==		==See Also==
-	*[[Malate ~~dehydrogenase~~\|Malate ~~dehydrogenase~~]]	+	*[[Malate Dehydrogenase 3D structures\|Malate Dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Crypv]]		[[Category: Crypv]]
		+	[[Category: Large Structures]]
	[[Category: Amani, M]]		[[Category: Amani, M]]
	[[Category: Arrowsmith, C H]]		[[Category: Arrowsmith, C H]]

OCA at 06:20, 27 June 2018

2018-06-27T06:20:32Z

←Older revision		Revision as of 06:20, 27 June 2018
Line 1:		Line 1:
		+
	==Crystal Structure of Cryptosporidium parvum malate dehydrogenase==		==Crystal Structure of Cryptosporidium parvum malate dehydrogenase==
	<StructureSection load='2hjr' size='340' side='right' caption='[[2hjr]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2hjr' size='340' side='right' caption='[[2hjr]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2hjr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Crypv Crypv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJR FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hjr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Crypv Crypv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hjr OCA], [http://pdbe.org/2hjr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hjr RCSB], [http://www.ebi.ac.uk/pdbsum/2hjr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hjr OCA], [http://pdbe.org/2hjr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hjr RCSB], [http://www.ebi.ac.uk/pdbsum/2hjr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hjr ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/2hjr_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/2hjr_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:25, 7 February 2016

2016-02-07T20:25:35Z

←Older revision		Revision as of 20:25, 7 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hjr ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:43, 10 September 2015

2015-09-10T04:43:07Z

←Older revision		Revision as of 04:43, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2hjr' size='340' side='right' caption='[[2hjr]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2hjr' size='340' side='right' caption='[[2hjr]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hjr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Cryptosporidium_parvum Cryptosporidium parvum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hjr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Crypv Crypv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hjr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hjr RCSB], [http://www.ebi.ac.uk/pdbsum/2hjr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hjr OCA], [http://pdbe.org/2hjr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hjr RCSB], [http://www.ebi.ac.uk/pdbsum/2hjr PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2hjr" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cryptosporidium parvum~~]]	+	[[Category: Crypv]]
	[[Category: Amani, M]]		[[Category: Amani, M]]
	[[Category: Arrowsmith, C H]]		[[Category: Arrowsmith, C H]]
Line 47:		Line 48:
	[[Category: Weigelt, J]]		[[Category: Weigelt, J]]
	[[Category: Wernimont, A K]]		[[Category: Wernimont, A K]]
		+	[[Category: Cryptosporidium parvum]]
	[[Category: Malaria]]		[[Category: Malaria]]
	[[Category: Malate dehydrogenase]]		[[Category: Malate dehydrogenase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Sgc]]		[[Category: Sgc]]

OCA at 08:57, 16 January 2015

2015-01-16T08:57:32Z

←Older revision		Revision as of 08:57, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hjr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJR FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hjr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJR FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hjr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hjr RCSB], [http://www.ebi.ac.uk/pdbsum/2hjr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hjr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hjr RCSB], [http://www.ebi.ac.uk/pdbsum/2hjr PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Cryptosporidium parvum]]		[[Category: Cryptosporidium parvum]]
-	[[Category: Amani, M.]]	+	[[Category: Amani, M]]
-	[[Category: Arrowsmith, C H.]]	+	[[Category: Arrowsmith, C H]]
-	[[Category: Bochkarev, A.]]	+	[[Category: Bochkarev, A]]
-	[[Category: Dong, A.]]	+	[[Category: Dong, A]]
-	[[Category: Edwards, A M.]]	+	[[Category: Edwards, A M]]
-	[[Category: Hassani, A.]]	+	[[Category: Hassani, A]]
-	[[Category: Hui, R.]]	+	[[Category: Hui, R]]
-	[[Category: Kozieradzki, I.]]	+	[[Category: Kozieradzki, I]]
-	[[Category: Lew, J.]]	+	[[Category: Lew, J]]
-	[[Category: Qiu, W.]]	+	[[Category: Qiu, W]]
-	[[Category: Ren, H.]]	+	[[Category: Ren, H]]
-	[[Category: ~~SGC,~~ Structural ~~Genomics Consortium.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Sundstrom, M.]]	+	[[Category: Sundstrom, M]]
-	[[Category: Weigelt, J.]]	+	[[Category: Weigelt, J]]
-	[[Category: Wernimont, A K~~.]]~~	+	[[Category: Wernimont, A K]]
-	~~[[Category: Cryptosporidium parvum~~]]	+
	[[Category: Malaria]]		[[Category: Malaria]]
	[[Category: Malate dehydrogenase]]		[[Category: Malate dehydrogenase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Sgc]]		[[Category: Sgc]]
-	[[Category: Structural genomic]]
-	[[Category: Structural genomics consortium]]

OCA at 05:19, 3 October 2014

2014-10-03T05:19:12Z

←Older revision		Revision as of 05:19, 3 October 2014
Line 1:		Line 1:
-	[[~~Image~~:2hjr.~~png\|left~~\|200px]]	+	==Crystal Structure of Cryptosporidium parvum malate dehydrogenase==
		+	<StructureSection load='2hjr' size='340' side='right' caption='[[2hjr]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2hjr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HJR FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hjr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hjr RCSB], [http://www.ebi.ac.uk/pdbsum/2hjr PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/2hjr_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Parasites from the protozoan phylum Apicomplexa are responsible for diseases, such as malaria, toxoplasmosis and cryptosporidiosis, all of which have significantly higher rates of mortality and morbidity in economically underdeveloped regions of the world. Advances in vaccine development and drug discovery are urgently needed to control these diseases and can be facilitated by production of purified recombinant proteins from Apicomplexan genomes and determination of their 3D structures. To date, both heterologous expression and crystallization of Apicomplexan proteins have seen only limited success. In an effort to explore the effectiveness of producing and crystallizing proteins on a genome-scale using a standardized methodology, over 400 distinct Plasmodium falciparum target genes were chosen representing different cellular classes, along with select orthologues from four other Plasmodium species as well as Cryptosporidium parvum and Toxoplasma gondii. From a total of 1008 genes from the seven genomes, 304 (30.2%) produced purified soluble proteins and 97 (9.6%) crystallized, culminating in 36 crystal structures. These results demonstrate that, contrary to previous findings, a standardized platform using Escherichia coli can be effective for genome-scale production and crystallography of Apicomplexan proteins. Predictably, orthologous proteins from different Apicomplexan genomes behaved differently in expression, purification and crystallization, although the overall success rates of Plasmodium orthologues do not differ significantly. Their differences were effectively exploited to elevate the overall productivity to levels comparable to the most successful ongoing structural genomics projects: 229 of the 468 target genes produced purified soluble protein from one or more organisms, with 80 and 32 of the purified targets, respectively, leading to crystals and ultimately structures from one or more orthologues.

-	~~{{STRUCTURE_2hjr\| PDB=2hjr \| SCENE= }}~~	+	Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms.,Vedadi M, Lew J, Artz J, Amani M, Zhao Y, Dong A, Wasney GA, Gao M, Hills T, Brokx S, Qiu W, Sharma S, Diassiti A, Alam Z, Melone M, Mulichak A, Wernimont A, Bray J, Loppnau P, Plotnikova O, Newberry K, Sundararajan E, Houston S, Walker J, Tempel W, Bochkarev A, Kozieradzki I, Edwards A, Arrowsmith C, Roos D, Kain K, Hui R Mol Biochem Parasitol. 2007 Jan;151(1):100-10. Epub 2006 Nov 13. PMID:17125854<ref>PMID:17125854</ref>

-	~~===Crystal Structure~~ of ~~Cryptosporidium parvum malate dehydrogenase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17125854}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2hjr]] is a 12 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA].~~	+

	==See Also==		==See Also==
-	*[[Johnson sandbox 1\|Johnson sandbox 1]]
	*[[Malate dehydrogenase\|Malate dehydrogenase]]		*[[Malate dehydrogenase\|Malate dehydrogenase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:017125854<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Cryptosporidium parvum]]		[[Category: Cryptosporidium parvum]]
	[[Category: Amani, M.]]		[[Category: Amani, M.]]

OCA at 07:46, 20 October 2012

2012-10-20T07:46:35Z

←Older revision		Revision as of 07:46, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2hjr]] is a 12 chain structure ~~of [[Johnson sandbox 1]] and [[Malate dehydrogenase]]~~ with sequence from [http://en.wikipedia.org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA].	+	[[2hjr]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA].

	==See Also==		==See Also==

OCA at 17:24, 25 July 2012

2012-07-25T17:24:24Z

←Older revision		Revision as of 17:24, 25 July 2012
Line 1:		Line 1:
	[[Image:2hjr.png\|left\|200px]]		[[Image:2hjr.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2hjr\| PDB=2hjr \| SCENE= }}		{{STRUCTURE_2hjr\| PDB=2hjr \| SCENE= }}

	===Crystal Structure of Cryptosporidium parvum malate dehydrogenase===		===Crystal Structure of Cryptosporidium parvum malate dehydrogenase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17125854}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17125854 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17125854}}		{{ABSTRACT_PUBMED_17125854}}

	==About this Structure==		==About this Structure==
-	[[2hjr]] is a 12 chain structure of [[Malate dehydrogenase]] with sequence from [http://en.wikipedia.org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA].	+	[[2hjr]] is a 12 chain structure of [[Johnson sandbox 1]] and [[Malate dehydrogenase]] with sequence from [http://en.wikipedia.org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJR OCA].

	==See Also==		==See Also==
-	*[[Malate dehydrogenase]]	+	*[[Johnson sandbox 1\|Johnson sandbox 1]]
		+	*[[Malate dehydrogenase\|Malate dehydrogenase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17125854~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017125854</ref><references group="xtra"/>
	[[Category: Cryptosporidium parvum]]		[[Category: Cryptosporidium parvum]]
	[[Category: Amani, M.]]		[[Category: Amani, M.]]