2hmk - Revision history

OCA at 09:59, 30 August 2023

2023-08-30T09:59:15Z

←Older revision		Revision as of 09:59, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='2hmk' size='340' side='right'caption='[[2hmk]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='2hmk' size='340' side='right'caption='[[2hmk]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hmk]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Achromobacter_georgiopolitanum Achromobacter georgiopolitanum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2HMK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hmk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HMK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=PEY:PHENANTHRENE'>PEY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o7h\|1o7h]], [[1o7g\|1o7g]], [[1o7m\|1o7m]], [[1o7n\|1o7n]], [[1o7p\|1o7p]], [[1o7w\|1o7w]], [[1uuv\|1uuv]], [[1uuw\|1uuw]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=PEY:PHENANTHRENE'>PEY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">doxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Achromobacter georgiopolitanum]), doxD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Achromobacter georgiopolitanum])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hmk OCA], [https://pdbe.org/2hmk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hmk RCSB], [https://www.ebi.ac.uk/pdbsum/2hmk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hmk ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naphthalene_1,2-dioxygenase Naphthalene 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.12 1.14.12.12] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2hmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hmk OCA], [~~http~~://pdbe.org/2hmk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2hmk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2hmk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2hmk ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NDOB_PSEU8 NDOB_PSEU8]] Component of the naphthalene dioxygenase (NDO) multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into naphthalene to form cis-naphthalene dihydrodiol. [[http://www.uniprot.org/uniprot/NDOC_PSEU8 NDOC_PSEU8]] Component of the naphthalene dioxygenase (NDO) multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into naphthalene to form cis-naphthalene dihydrodiol. The beta subunit may be responsible for the substrate specificity of the enzyme.	+	[https://www.uniprot.org/uniprot/NDOB_PSEU8 NDOB_PSEU8] Component of the naphthalene dioxygenase (NDO) multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into naphthalene to form cis-naphthalene dihydrodiol.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Achromobacter georgiopolitanum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Naphthalene 1,2-dioxygenase~~]]	+	[[Category: Pseudomonas sp]]
-	[[Category: Ferraro~~, D J~~]]	+	[[Category: Ferraro DJ]]
-	[[Category: Mowers~~, J C~~]]	+	[[Category: Mowers JC]]
-	[[Category: Okerlund~~, A L~~]]	+	[[Category: Okerlund AL]]
-	[[Category: Ramaswamy, S]]	+	[[Category: Ramaswamy S]]
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Protein]]~~	+
-	~~[[Category: Rieske oxygenase~~]]	+

OCA at 06:56, 2 September 2020

2020-09-02T06:56:35Z

←Older revision		Revision as of 06:56, 2 September 2020
Line 1:		Line 1:

	==Crystal Structure of Naphthalene 1,2-Dioxygenase Bound to Phenanthrene==		==Crystal Structure of Naphthalene 1,2-Dioxygenase Bound to Phenanthrene==
-	<StructureSection load='2hmk' size='340' side='right' caption='[[2hmk]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='2hmk' size='340' side='right'caption='[[2hmk]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hmk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Achromobacter_georgiopolitanum Achromobacter georgiopolitanum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HMK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hmk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Achromobacter_georgiopolitanum Achromobacter georgiopolitanum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2HMK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=PEY:PHENANTHRENE'>PEY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=PEY:PHENANTHRENE'>PEY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o7h\|1o7h]], [[1o7g\|1o7g]], [[1o7m\|1o7m]], [[1o7n\|1o7n]], [[1o7p\|1o7p]], [[1o7w\|1o7w]], [[1uuv\|1uuv]], [[1uuw\|1uuw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o7h\|1o7h]], [[1o7g\|1o7g]], [[1o7m\|1o7m]], [[1o7n\|1o7n]], [[1o7p\|1o7p]], [[1o7w\|1o7w]], [[1uuv\|1uuv]], [[1uuw\|1uuw]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">doxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Achromobacter georgiopolitanum]), doxD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Achromobacter georgiopolitanum])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">doxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Achromobacter georgiopolitanum]), doxD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Achromobacter georgiopolitanum])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naphthalene_1,2-dioxygenase Naphthalene 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.12 1.14.12.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naphthalene_1,2-dioxygenase Naphthalene 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.12 1.14.12.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2hmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hmk OCA], [http://pdbe.org/2hmk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hmk RCSB], [http://www.ebi.ac.uk/pdbsum/2hmk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hmk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2hmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hmk OCA], [http://pdbe.org/2hmk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hmk RCSB], [http://www.ebi.ac.uk/pdbsum/2hmk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hmk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:

	==See Also==		==See Also==
-	*[[Dioxygenase\|Dioxygenase]]	+	*[[Dioxygenase 3D structures\|Dioxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 39:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Achromobacter georgiopolitanum]]		[[Category: Achromobacter georgiopolitanum]]
		+	[[Category: Large Structures]]
	[[Category: Naphthalene 1,2-dioxygenase]]		[[Category: Naphthalene 1,2-dioxygenase]]
	[[Category: Ferraro, D J]]		[[Category: Ferraro, D J]]
Line 45:		Line 46:
	[[Category: Ramaswamy, S]]		[[Category: Ramaswamy, S]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-	[[Category: ~~Oxioreductase]]~~	+	[[Category: Protein]]
-	~~[[Category: Protien~~]]	+
	[[Category: Rieske oxygenase]]		[[Category: Rieske oxygenase]]

OCA at 06:31, 27 June 2018

2018-06-27T06:31:03Z

←Older revision		Revision as of 06:31, 27 June 2018
Line 1:		Line 1:
		+
	==Crystal Structure of Naphthalene 1,2-Dioxygenase Bound to Phenanthrene==		==Crystal Structure of Naphthalene 1,2-Dioxygenase Bound to Phenanthrene==
	<StructureSection load='2hmk' size='340' side='right' caption='[[2hmk]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='2hmk' size='340' side='right' caption='[[2hmk]], [[Resolution\|resolution]] 1.65Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">doxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Achromobacter georgiopolitanum]), doxD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Achromobacter georgiopolitanum])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">doxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Achromobacter georgiopolitanum]), doxD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Achromobacter georgiopolitanum])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naphthalene_1,2-dioxygenase Naphthalene 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.12 1.14.12.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naphthalene_1,2-dioxygenase Naphthalene 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.12 1.14.12.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hmk OCA], [http://pdbe.org/2hmk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hmk RCSB], [http://www.ebi.ac.uk/pdbsum/2hmk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hmk OCA], [http://pdbe.org/2hmk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hmk RCSB], [http://www.ebi.ac.uk/pdbsum/2hmk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hmk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hm/2hmk_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hm/2hmk_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hmk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:42, 11 September 2015

2015-09-11T10:42:21Z

←Older revision		Revision as of 10:42, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2hmk' size='340' side='right' caption='[[2hmk]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='2hmk' size='340' side='right' caption='[[2hmk]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hmk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_sp. Pseudomonas sp.~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HMK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hmk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Achromobacter_georgiopolitanum Achromobacter georgiopolitanum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HMK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=PEY:PHENANTHRENE'>PEY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=PEY:PHENANTHRENE'>PEY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o7h\|1o7h]], [[1o7g\|1o7g]], [[1o7m\|1o7m]], [[1o7n\|1o7n]], [[1o7p\|1o7p]], [[1o7w\|1o7w]], [[1uuv\|1uuv]], [[1uuw\|1uuw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o7h\|1o7h]], [[1o7g\|1o7g]], [[1o7m\|1o7m]], [[1o7n\|1o7n]], [[1o7p\|1o7p]], [[1o7w\|1o7w]], [[1uuv\|1uuv]], [[1uuw\|1uuw]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">doxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 ~~Pseudomonas sp.~~]), doxD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 ~~Pseudomonas sp.~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">doxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Achromobacter georgiopolitanum]), doxD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Achromobacter georgiopolitanum])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naphthalene_1,2-dioxygenase Naphthalene 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.12 1.14.12.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naphthalene_1,2-dioxygenase Naphthalene 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.12 1.14.12.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hmk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hmk RCSB], [http://www.ebi.ac.uk/pdbsum/2hmk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hmk OCA], [http://pdbe.org/2hmk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hmk RCSB], [http://www.ebi.ac.uk/pdbsum/2hmk PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2hmk" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Achromobacter georgiopolitanum]]
	[[Category: Naphthalene 1,2-dioxygenase]]		[[Category: Naphthalene 1,2-dioxygenase]]
-	[[Category: Pseudomonas sp]]
	[[Category: Ferraro, D J]]		[[Category: Ferraro, D J]]
	[[Category: Mowers, J C]]		[[Category: Mowers, J C]]

OCA at 15:22, 25 December 2014

2014-12-25T15:22:31Z

←Older revision		Revision as of 15:22, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hmk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HMK FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hmk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HMK FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=PEY:PHENANTHRENE'>PEY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=PEY:PHENANTHRENE'>PEY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o7h\|1o7h]], [[1o7g\|1o7g]], [[1o7m\|1o7m]], [[1o7n\|1o7n]], [[1o7p\|1o7p]], [[1o7w\|1o7w]], [[1uuv\|1uuv]], [[1uuw\|1uuw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o7h\|1o7h]], [[1o7g\|1o7g]], [[1o7m\|1o7m]], [[1o7n\|1o7n]], [[1o7p\|1o7p]], [[1o7w\|1o7w]], [[1uuv\|1uuv]], [[1uuw\|1uuw]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">doxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Pseudomonas sp.]), doxD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Pseudomonas sp.])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">doxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Pseudomonas sp.]), doxD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Pseudomonas sp.])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naphthalene_1,2-dioxygenase Naphthalene 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.12 1.14.12.12] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naphthalene_1,2-dioxygenase Naphthalene 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.12 1.14.12.12] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hmk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hmk RCSB], [http://www.ebi.ac.uk/pdbsum/2hmk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hmk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hmk RCSB], [http://www.ebi.ac.uk/pdbsum/2hmk PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NDOB_PSEU8 NDOB_PSEU8]] Component of the naphthalene dioxygenase (NDO) multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into naphthalene to form cis-naphthalene dihydrodiol. [[http://www.uniprot.org/uniprot/NDOC_PSEU8 NDOC_PSEU8]] Component of the naphthalene dioxygenase (NDO) multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into naphthalene to form cis-naphthalene dihydrodiol. The beta subunit may be responsible for the substrate specificity of the enzyme.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Naphthalene 1,2-dioxygenase]]		[[Category: Naphthalene 1,2-dioxygenase]]
-	[[Category: Pseudomonas sp.]]	+	[[Category: Pseudomonas sp]]
-	[[Category: Ferraro, D J.]]	+	[[Category: Ferraro, D J]]
-	[[Category: Mowers, J C.]]	+	[[Category: Mowers, J C]]
-	[[Category: Okerlund, A L.]]	+	[[Category: Okerlund, A L]]
-	[[Category: Ramaswamy, S.]]	+	[[Category: Ramaswamy, S]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Oxioreductase]]		[[Category: Oxioreductase]]
	[[Category: Protien]]		[[Category: Protien]]
	[[Category: Rieske oxygenase]]		[[Category: Rieske oxygenase]]

OCA at 07:59, 30 September 2014

2014-09-30T07:59:02Z

←Older revision		Revision as of 07:59, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2hmk.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of Naphthalene 1,2-Dioxygenase Bound to Phenanthrene==
		+	<StructureSection load='2hmk' size='340' side='right' caption='[[2hmk]], [[Resolution\|resolution]] 1.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2hmk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HMK FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=PEY:PHENANTHRENE'>PEY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1o7h\|1o7h]], [[1o7g\|1o7g]], [[1o7m\|1o7m]], [[1o7n\|1o7n]], [[1o7p\|1o7p]], [[1o7w\|1o7w]], [[1uuv\|1uuv]], [[1uuw\|1uuw]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">doxB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Pseudomonas sp.]), doxD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=306 Pseudomonas sp.])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naphthalene_1,2-dioxygenase Naphthalene 1,2-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.12.12 1.14.12.12] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hmk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hmk RCSB], [http://www.ebi.ac.uk/pdbsum/2hmk PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hm/2hmk_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rieske oxygenase (RO) systems are two- and three-component enzyme systems that catalyze the formation of cis-dihydrodiols from aromatic substrates. Degradation of pollutants in contaminated soil and generation of chiral synthons have been the major foci of RO research. Substrate specificity and product regio- and stereoselectivity have been shown to vary between individual ROs. While directed evolution methods for altering RO function have been successful in the past, rational engineering of these enzymes still poses a challenge due to the lack of structural understanding. Here we examine the structural changes induced by mutation of Phe-352 in naphthalene 1,2-dioxygenase from Pseudomonas sp. strain NCIB 9816-4 (NDO-O(9816-4)). Structures of the Phe-352-Val mutant in native form and in complex with phenanthrene and anthracene, along with those of wild-type NDO-O(9816-4) in complex with phenanthrene, anthracene, and 3-nitrotoluene, are presented. Phenanthrene was shown to bind in a different orientation in the Phe-352-Val mutant active site from that in the wild type, while anthracene was found to bind in similar positions in both enzymes. Two orientations of 3-nitrotoluene were observed, i.e., a productive and a nonproductive orientation. These orientations help explain why NDO-O(9816-4) forms different products from 3-nitrotoluene than those made from nitrobenzene dioxygenase. Comparison of these structures among themselves and with other known ROs bound to substrates reveals that the orientation of substrate binding at the active site is the primary determinant of product regio- and stereoselectivity.

-	~~{{STRUCTURE_2hmk\| PDB=2hmk \| SCENE= }}~~	+	Structural basis for regioselectivity and stereoselectivity of product formation by naphthalene 1,2-dioxygenase.,Ferraro DJ, Okerlund AL, Mowers JC, Ramaswamy S J Bacteriol. 2006 Oct;188(19):6986-94. PMID:16980501<ref>PMID:16980501</ref>

-	~~===Crystal Structure of Naphthalene 1~~,~~2-Dioxygenase Bound to Phenanthrene===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_16980501}}~~	+	==See Also==
-		+	*[[Dioxygenase\|Dioxygenase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~2hmk~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:016980501<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Naphthalene 1,2-dioxygenase]]		[[Category: Naphthalene 1,2-dioxygenase]]
	[[Category: Pseudomonas sp.]]		[[Category: Pseudomonas sp.]]

OCA: Protected "2hmk" [edit=sysop:move=sysop]

2013-01-07T18:02:18Z

Protected "2hmk" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:02, 7 January 2013

OCA at 18:02, 7 January 2013

2013-01-07T18:02:15Z

←Older revision		Revision as of 18:02, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2hmk.png\|left\|200px]]		[[Image:2hmk.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2hmk", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2hmk\| PDB=2hmk \| SCENE= }}		{{STRUCTURE_2hmk\| PDB=2hmk \| SCENE= }}

	===Crystal Structure of Naphthalene 1,2-Dioxygenase Bound to Phenanthrene===		===Crystal Structure of Naphthalene 1,2-Dioxygenase Bound to Phenanthrene===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16980501}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16980501 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16980501}}		{{ABSTRACT_PUBMED_16980501}}

	==About this Structure==		==About this Structure==
-	~~2HMK~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA].	+	[[2hmk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16980501~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016980501</ref><references group="xtra"/>
	[[Category: Naphthalene 1,2-dioxygenase]]		[[Category: Naphthalene 1,2-dioxygenase]]
	[[Category: Pseudomonas sp.]]		[[Category: Pseudomonas sp.]]
Line 30:		Line 18:
	[[Category: Okerlund, A L.]]		[[Category: Okerlund, A L.]]
	[[Category: Ramaswamy, S.]]		[[Category: Ramaswamy, S.]]
		+	[[Category: Oxidoreductase]]
	[[Category: Oxioreductase]]		[[Category: Oxioreductase]]
	[[Category: Protien]]		[[Category: Protien]]
	[[Category: Rieske oxygenase]]		[[Category: Rieske oxygenase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:11:28 2009''

OCA at 18:11, 17 February 2009

2009-02-17T18:11:30Z

←Older revision		Revision as of 18:11, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2HMK is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA].	+	2HMK is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMK OCA].

	==Reference==		==Reference==
-	Structural basis for regioselectivity and stereoselectivity of product formation by naphthalene 1,2-dioxygenase., Ferraro DJ, Okerlund AL, Mowers JC, Ramaswamy S, J Bacteriol. 2006 Oct;188(19):6986-94. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16980501 16980501]~~	+	<ref group="xtra">PMID:16980501</ref><references group="xtra"/>
	[[Category: Naphthalene 1,2-dioxygenase]]		[[Category: Naphthalene 1,2-dioxygenase]]
-	[[Category: Protein complex]]
	[[Category: Pseudomonas sp.]]		[[Category: Pseudomonas sp.]]
	[[Category: Ferraro, D J.]]		[[Category: Ferraro, D J.]]
Line 35:		Line 34:
	[[Category: Rieske oxygenase]]		[[Category: Rieske oxygenase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 04~~:02:~~54 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:11:28 2009''

OCA at 01:02, 29 July 2008

2008-07-29T01:02:58Z

←Older revision		Revision as of 01:02, 29 July 2008
Line 1:		Line 1:
-	[[Image:2hmk.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2hmk.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2hmk\| PDB=2hmk \| SCENE= }}		{{STRUCTURE_2hmk\| PDB=2hmk \| SCENE= }}

-	~~'''~~Crystal Structure of Naphthalene 1,2-Dioxygenase Bound to Phenanthrene~~'''~~	+	===Crystal Structure of Naphthalene 1,2-Dioxygenase Bound to Phenanthrene===


-	~~==Overview==~~	+	<!--
-	~~Rieske oxygenase (RO) systems are two~~- ~~and three~~-~~component enzyme systems that catalyze~~ the formation of cis-dihydrodiols from aromatic substrates. Degradation of pollutants in contaminated soil and generation of chiral synthons have been the major foci of RO research. Substrate specificity and product regio- and stereoselectivity have been shown to ~~vary between individual ROs. While directed evolution methods for altering RO function have been successful in~~ the ~~past, rational engineering of these enzymes still poses a challenge due to the lack of structural understanding~~. ~~Here we examine the structural changes induced by mutation of Phe-352 in naphthalene 1,2-dioxygenase from Pseudomonas sp~~. ~~strain NCIB 9816-4 (NDO-O(9816-4~~)~~). Structures of the Phe-352-Val mutant in native form and in complex with phenanthrene and anthracene~~, ~~along with those of wild-type NDO-O(9816-4) in complex with phenanthrene, anthracene, and 3-nitrotoluene, are presented. Phenanthrene was shown to bind in a different orientation in~~ the ~~Phe-352-Val mutant active site from that in the wild type, while anthracene was found to bind in similar positions in both enzymes~~. ~~Two orientations of 3~~-~~nitrotoluene were observed, i.e., a productive and a nonproductive orientation. These orientations help explain why NDO~~-O(9816-4) forms different products from 3-nitrotoluene than those made from nitrobenzene dioxygenase. Comparison of these structures among themselves and with other known ROs bound to substrates reveals that the orientation of substrate binding at the active site is the primary determinant of product regio- and stereoselectivity.	+	The line below this paragraph, {{ABSTRACT_PUBMED_16980501}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 16980501 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_16980501}}

	==About this Structure==		==About this Structure==
Line 30:		Line 34:
	[[Category: Protien]]		[[Category: Protien]]
	[[Category: Rieske oxygenase]]		[[Category: Rieske oxygenase]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 06~~:27:51 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:02:54 2008''