2hp9 - Revision history

OCA at 10:00, 30 August 2023

2023-08-30T10:00:20Z

←Older revision		Revision as of 10:00, 30 August 2023
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	<StructureSection load='2hp9' size='340' side='right'caption='[[2hp9]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2hp9' size='340' side='right'caption='[[2hp9]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hp9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HP9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hp9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HP9 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[1k4f\|1k4f]]~~, [[~~2hp5~~\|~~2hp5]], [[2hp6\|2hp6]], [[2hpb\|2hpb~~]]~~</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>pse2, oxa10 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~287~~ "~~Bacillus aeruginosus~~" ~~(Schroeter 1872) Trevisan 1885])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hp9 OCA], [https://pdbe.org/2hp9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hp9 RCSB], [https://www.ebi.ac.uk/pdbsum/2hp9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hp9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hp9 OCA], [https://pdbe.org/2hp9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hp9 RCSB], [https://www.ebi.ac.uk/pdbsum/2hp9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hp9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BLO10_PSEAI BLO10_PSEAI]] Hydrolyzes both carbenicillin and oxacillin.	+	[https://www.uniprot.org/uniprot/BLO10_PSEAI BLO10_PSEAI] Hydrolyzes both carbenicillin and oxacillin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beta-lactamase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Charlier, P]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: Falzone, C]]	+	[[Category: Charlier P]]
-	[[Category: Herman, R]]	+	[[Category: Falzone C]]
-	[[Category: Kerff, F]]	+	[[Category: Herman R]]
-	[[Category: Sauvage, E]]	+	[[Category: Kerff F]]
-	~~[[Category: Class d beta-lactamase]]~~	+	[[Category: Sauvage E]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Lysine carboxylation~~]]	+

OCA at 07:19, 24 March 2021

2021-03-24T07:19:15Z

←Older revision		Revision as of 07:19, 24 March 2021
Line 1:		Line 1:

	==Crystal Structure of the OXA-10 W154A mutant at pH 6.0==		==Crystal Structure of the OXA-10 W154A mutant at pH 6.0==
-	<StructureSection load='2hp9' size='340' side='right' caption='[[2hp9]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='2hp9' size='340' side='right'caption='[[2hp9]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hp9]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HP9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hp9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HP9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k4f\|1k4f]], [[2hp5\|2hp5]], [[2hp6\|2hp6]], [[2hpb\|2hpb]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1k4f\|1k4f]], [[2hp5\|2hp5]], [[2hp6\|2hp6]], [[2hpb\|2hpb]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pse2, oxa10 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pse2, oxa10 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2hp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hp9 OCA], [~~http~~://pdbe.org/2hp9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2hp9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2hp9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2hp9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hp9 OCA], [https://pdbe.org/2hp9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hp9 RCSB], [https://www.ebi.ac.uk/pdbsum/2hp9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hp9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BLO10_PSEAI BLO10_PSEAI]] Hydrolyzes both carbenicillin and oxacillin.	+	[[https://www.uniprot.org/uniprot/BLO10_PSEAI BLO10_PSEAI]] Hydrolyzes both carbenicillin and oxacillin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:

	==See Also==		==See Also==
-	*[[Beta-lactamase\|Beta-lactamase]]	+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 39:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
		+	[[Category: Large Structures]]
	[[Category: Charlier, P]]		[[Category: Charlier, P]]
	[[Category: Falzone, C]]		[[Category: Falzone, C]]

OCA at 06:33, 27 June 2018

2018-06-27T06:33:57Z

←Older revision		Revision as of 06:33, 27 June 2018
Line 1:		Line 1:
		+
	==Crystal Structure of the OXA-10 W154A mutant at pH 6.0==		==Crystal Structure of the OXA-10 W154A mutant at pH 6.0==
	<StructureSection load='2hp9' size='340' side='right' caption='[[2hp9]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2hp9' size='340' side='right' caption='[[2hp9]], [[Resolution\|resolution]] 2.50Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pse2, oxa10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pse2, oxa10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hp9 OCA], [http://pdbe.org/2hp9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hp9 RCSB], [http://www.ebi.ac.uk/pdbsum/2hp9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hp9 OCA], [http://pdbe.org/2hp9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hp9 RCSB], [http://www.ebi.ac.uk/pdbsum/2hp9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hp9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/2hp9_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/2hp9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hp9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:08, 11 September 2015

2015-09-11T23:08:41Z

←Older revision		Revision as of 23:08, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2hp9' size='340' side='right' caption='[[2hp9]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2hp9' size='340' side='right' caption='[[2hp9]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hp9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HP9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hp9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HP9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k4f\|1k4f]], [[2hp5\|2hp5]], [[2hp6\|2hp6]], [[2hpb\|2hpb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k4f\|1k4f]], [[2hp5\|2hp5]], [[2hp6\|2hp6]], [[2hpb\|2hpb]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pse2, oxa10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 ~~Pseudomonas aeruginosa~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pse2, oxa10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hp9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hp9 RCSB], [http://www.ebi.ac.uk/pdbsum/2hp9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hp9 OCA], [http://pdbe.org/2hp9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hp9 RCSB], [http://www.ebi.ac.uk/pdbsum/2hp9 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BLO10_PSEAI BLO10_PSEAI]] Hydrolyzes both carbenicillin and oxacillin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2hp9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
-	[[Category: Pseudomonas aeruginosa]]
	[[Category: Charlier, P]]		[[Category: Charlier, P]]
	[[Category: Falzone, C]]		[[Category: Falzone, C]]

OCA at 09:10, 16 January 2015

2015-01-16T09:10:18Z

←Older revision		Revision as of 09:10, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hp9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HP9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hp9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HP9 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k4f\|1k4f]], [[2hp5\|2hp5]], [[2hp6\|2hp6]], [[2hpb\|2hpb]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k4f\|1k4f]], [[2hp5\|2hp5]], [[2hp6\|2hp6]], [[2hpb\|2hpb]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pse2, oxa10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pse2, oxa10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hp9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hp9 RCSB], [http://www.ebi.ac.uk/pdbsum/2hp9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hp9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hp9 RCSB], [http://www.ebi.ac.uk/pdbsum/2hp9 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
-	[[Category: Charlier, P.]]	+	[[Category: Charlier, P]]
-	[[Category: Falzone, C.]]	+	[[Category: Falzone, C]]
-	[[Category: Herman, R.]]	+	[[Category: Herman, R]]
-	[[Category: Kerff, F.]]	+	[[Category: Kerff, F]]
-	[[Category: Sauvage, E.]]	+	[[Category: Sauvage, E]]
	[[Category: Class d beta-lactamase]]		[[Category: Class d beta-lactamase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Lysine carboxylation]]		[[Category: Lysine carboxylation]]

OCA at 04:05, 29 September 2014

2014-09-29T04:05:05Z

←Older revision		Revision as of 04:05, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2hp9.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of the OXA-10 W154A mutant at pH 6.0==
		+	<StructureSection load='2hp9' size='340' side='right' caption='[[2hp9]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2hp9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HP9 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k4f\|1k4f]], [[2hp5\|2hp5]], [[2hp6\|2hp6]], [[2hpb\|2hpb]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pse2, oxa10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hp9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hp9 RCSB], [http://www.ebi.ac.uk/pdbsum/2hp9 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/2hp9_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The catalytic efficiency of class D OXA-10 beta-lactamase depends critically on an unusual carboxylated lysine as the general base residue for both the enzyme acylation and deacylation steps of catalysis. Evidence is presented that the interaction between the indole group of Trp154 and the carboxylated lysine is essential for the stability of the post-translationally modified Lys70. Substitution of Trp154 by Gly, Ala or Phe yielded non carboxylated enzymes which displayed poor catalytic efficiencies and reduced stability when compared to the wild type OXA-10. The W154H mutant was partially carboxylated. In addition, the maximum values of kcat and kcat/KM were shifted toward pH 7 indicating that the carboxylation state of Lys70 is dependent on the protonation level of the histidine. A comparison of the three dimensional structures of the different proteins also indicated that the Trp154 mutations did not modify the overall structures of OXA-10 but induced an increased flexibility of the omega-loop in the active site. Finally, the deacylation impaired W154A mutant was used to determine the structure of the acyl-enzyme complex with benzylpenicillin. These results indicate a role of the Lys70 carboxylation during the deacylation step and emphasize the importance of Trp154 for the ideal positioning of active site residues leading to an optimum activity.

-	~~{{STRUCTURE_2hp9\| PDB=2hp9 \| SCENE= }}~~	+	Critical role of Tryptophan 154 for the activity and stability of class D beta-lactamases.,Baurin S, Vercheval L, Bouillenne F, Falzone C, Brans A, Jacquamet L, Ferrer JL, Sauvage E, Dehareng D, Frere JM, Charlier P, Galleni M, Kerff F Biochemistry. 2009 Oct 27. PMID:19860471<ref>PMID:19860471</ref>

-	~~===Crystal Structure~~ of the ~~OXA-10 W154A mutant at pH 6~~.~~0===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19860471}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2hp9]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA].~~	+

	==See Also==		==See Also==
	*[[Beta-lactamase\|Beta-lactamase]]		*[[Beta-lactamase\|Beta-lactamase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019860471<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]

OCA at 21:21, 20 October 2012

2012-10-20T21:21:20Z

←Older revision		Revision as of 21:21, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2hp9]] is a 2 chain structure ~~of [[Beta-lactamase]]~~ with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA].	+	[[2hp9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA].

	==See Also==		==See Also==

OCA: Protected "2hp9" [edit=sysop:move=sysop]

2012-07-27T13:00:31Z

Protected "2hp9" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:00, 27 July 2012

OCA at 13:00, 27 July 2012

2012-07-27T13:00:30Z

←Older revision		Revision as of 13:00, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2hp9.png\|left\|200px]]		[[Image:2hp9.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2hp9", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2hp9\| PDB=2hp9 \| SCENE= }}		{{STRUCTURE_2hp9\| PDB=2hp9 \| SCENE= }}

	===Crystal Structure of the OXA-10 W154A mutant at pH 6.0===		===Crystal Structure of the OXA-10 W154A mutant at pH 6.0===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19860471}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19860471 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19860471}}		{{ABSTRACT_PUBMED_19860471}}

	==About this Structure==		==About this Structure==
-	~~2HP9~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA].	+	[[2hp9]] is a 2 chain structure of [[Beta-lactamase]] with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA].
		+
		+	==See Also==
		+	*[[Beta-lactamase\|Beta-lactamase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19860471~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019860471</ref><references group="xtra"/>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
Line 34:		Line 25:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Lysine carboxylation]]		[[Category: Lysine carboxylation]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Aug 12 00:02:18 2010''

OCA at 19:55, 11 August 2010

2010-08-11T19:55:02Z

←Older revision		Revision as of 19:55, 11 August 2010
Line 12:		Line 12:
	===Crystal Structure of the OXA-10 W154A mutant at pH 6.0===		===Crystal Structure of the OXA-10 W154A mutant at pH 6.0===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19860471}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19860471 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19860471}}

	==About this Structure==		==About this Structure==
-	2HP9 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA].	+	2HP9 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HP9 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19860471</ref><references group="xtra"/>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
Line 23:		Line 32:
	[[Category: Sauvage, E.]]		[[Category: Sauvage, E.]]
	[[Category: Class d beta-lactamase]]		[[Category: Class d beta-lactamase]]
		+	[[Category: Hydrolase]]
	[[Category: Lysine carboxylation]]		[[Category: Lysine carboxylation]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Feb 18 06~~:33:~~11 2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Aug 12 00:02:18 2010''