2i2h - Revision history

OCA at 16:38, 13 December 2023

2023-12-13T16:38:05Z

←Older revision		Revision as of 16:38, 13 December 2023
Line 1:		Line 1:

	==NMR structure of TPC3 in TFE==		==NMR structure of TPC3 in TFE==
-	<StructureSection load='2i2h' size='340' side='right'caption='[[2i2h~~]], [[NMR_Ensembles_of_Models \| 22 NMR models~~]]' scene=''>	+	<StructureSection load='2i2h' size='340' side='right'caption='[[2i2h]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i2h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I2H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i2h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I2H FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2h OCA], [https://pdbe.org/2i2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i2h RCSB], [https://www.ebi.ac.uk/pdbsum/2i2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i2h ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2h OCA], [https://pdbe.org/2i2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i2h RCSB], [https://www.ebi.ac.uk/pdbsum/2i2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i2h ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9APK6_STRMG Q9APK6_STRMG] Acts as a pheromone, induces cells to develop competence for genetic transformation.[ARBA:ARBA00002667]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Jakeman, D L~~]]	+	[[Category: Streptococcus mutans]]
-	[[Category: ~~Li, Y~~]]	+	[[Category: Jakeman DL]]
-	[[Category: ~~Syvitski, R T~~]]	+	[[Category: Li Y]]
-	[[Category: ~~Helix]]~~	+	[[Category: Syvitski RT]]
-	~~[[Category: Signaling protein~~]]	+

OCA at 10:24, 12 January 2022

2022-01-12T10:24:32Z

←Older revision		Revision as of 10:24, 12 January 2022
Line 3:		Line 3:
	<StructureSection load='2i2h' size='340' side='right'caption='[[2i2h]], [[NMR_Ensembles_of_Models \| 22 NMR models]]' scene=''>		<StructureSection load='2i2h' size='340' side='right'caption='[[2i2h]], [[NMR_Ensembles_of_Models \| 22 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i2h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2I2H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i2h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I2H FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2i2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2h OCA], [~~http~~://pdbe.org/2i2h PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2i2h RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2i2h PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2i2h ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2h OCA], [https://pdbe.org/2i2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i2h RCSB], [https://www.ebi.ac.uk/pdbsum/2i2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i2h ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:41, 8 April 2020

2020-04-08T07:41:46Z

←Older revision		Revision as of 07:41, 8 April 2020
Line 1:		Line 1:

	==NMR structure of TPC3 in TFE==		==NMR structure of TPC3 in TFE==
-	<StructureSection load='2i2h' size='340' side='right' caption='[[2i2h]], [[NMR_Ensembles_of_Models \| 22 NMR models]]' scene=''>	+	<StructureSection load='2i2h' size='340' side='right'caption='[[2i2h]], [[NMR_Ensembles_of_Models \| 22 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i2h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2I2H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i2h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2I2H FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2i2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2h OCA], [http://pdbe.org/2i2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i2h RCSB], [http://www.ebi.ac.uk/pdbsum/2i2h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i2h ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2i2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2h OCA], [http://pdbe.org/2i2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i2h RCSB], [http://www.ebi.ac.uk/pdbsum/2i2h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i2h ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Jakeman, D L]]		[[Category: Jakeman, D L]]
	[[Category: Li, Y]]		[[Category: Li, Y]]

OCA at 07:50, 4 July 2018

2018-07-04T07:50:06Z

←Older revision		Revision as of 07:50, 4 July 2018
Line 1:		Line 1:
		+
	==NMR structure of TPC3 in TFE==		==NMR structure of TPC3 in TFE==
	<StructureSection load='2i2h' size='340' side='right' caption='[[2i2h]], [[NMR_Ensembles_of_Models \| 22 NMR models]]' scene=''>		<StructureSection load='2i2h' size='340' side='right' caption='[[2i2h]], [[NMR_Ensembles_of_Models \| 22 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2i2h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I2H FirstGlance]. <br>		<table><tr><td colspan='2'>[[2i2h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I2H FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2h OCA], [http://pdbe.org/2i2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i2h RCSB], [http://www.ebi.ac.uk/pdbsum/2i2h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2h OCA], [http://pdbe.org/2i2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i2h RCSB], [http://www.ebi.ac.uk/pdbsum/2i2h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i2h ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:27, 9 September 2015

2015-09-09T23:27:48Z

←Older revision		Revision as of 23:27, 9 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[2i2h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I2H FirstGlance]. <br>		<table><tr><td colspan='2'>[[2i2h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I2H FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i2h RCSB], [http://www.ebi.ac.uk/pdbsum/2i2h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2h OCA], [http://pdbe.org/2i2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i2h RCSB], [http://www.ebi.ac.uk/pdbsum/2i2h PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2i2h" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:47, 16 January 2015

2015-01-16T08:47:02Z

←Older revision		Revision as of 08:47, 16 January 2015
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Jakeman, D L.]]	+	[[Category: Jakeman, D L]]
-	[[Category: Li, Y.]]	+	[[Category: Li, Y]]
-	[[Category: Syvitski, R T.]]	+	[[Category: Syvitski, R T]]
	[[Category: Helix]]		[[Category: Helix]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 15:25, 12 October 2014

2014-10-12T15:25:47Z

←Older revision		Revision as of 15:25, 12 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2i2h.~~png~~\|~~left\|200px~~]]	+	==NMR structure of TPC3 in TFE==
		+	<StructureSection load='2i2h' size='340' side='right' caption='[[2i2h]], [[NMR_Ensembles_of_Models \| 22 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2i2h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I2H FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i2h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i2h RCSB], [http://www.ebi.ac.uk/pdbsum/2i2h PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Streptococcus mutans secretes and utilizes a 21-amino-acid signaling peptide pheromone to initiate quorum sensing for genetic competence, biofilm formation, stress responses, and bacteriocin production. In this study, we designed and synthesized a series of truncated peptides and peptides with amino acid substitutions to investigate their structure-activity relationships based on the three-dimensional structures of S. mutans wild-type signaling peptide UA159sp and C-terminally truncated peptide TPC3 from mutant JH1005 defective in genetic competence. By analyzing these peptides, we demonstrated that the signaling peptide of S. mutans has at least two functional domains. The C-terminal structural motif consisting of a sequence of polar hydrophobic charged residues is crucial for activation of the signal transduction pathway, while the core alpha-helical structure extending from residue 5 to the end of the peptide is required for receptor binding. Peptides in which three or more residues were deleted from the C terminus did not induce genetic competence but competitively inhibited quorum sensing activated by UA159sp. Disruption of the amphipathic alpha-helix by replacing the Phe-7, Phe-11, or Phe-15 residue with a hydrophilic residue resulted in a significant reduction in or complete loss of the activity of the peptide. In contrast to the C-terminally truncated peptides, these peptides with amino acid substitutions did not compete with UA159sp to activate quorum sensing, suggesting that disruption of the hydrophobic face of the alpha-helical structure results in a peptide that is not able to bind to the receptor. This study is the first study to recognize the importance of the signaling peptide C-terminal residues in streptococcal quorum sensing.

-	~~{{STRUCTURE_2i2h\| PDB=2i2h \| SCENE= }}~~	+	Structure-activity analysis of quorum-sensing signaling peptides from Streptococcus mutans.,Syvitski RT, Tian XL, Sampara K, Salman A, Lee SF, Jakeman DL, Li YH J Bacteriol. 2007 Feb;189(4):1441-50. Epub 2006 Aug 25. PMID:16936029<ref>PMID:16936029</ref>

-	~~===NMR structure~~ of ~~TPC3 in TFE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16936029}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2i2h]] is a 1 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2I2H OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016936029<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Jakeman, D L.]]		[[Category: Jakeman, D L.]]
	[[Category: Li, Y.]]		[[Category: Li, Y.]]

OCA: Protected "2i2h" [edit=sysop:move=sysop]

2013-01-07T12:10:22Z

Protected "2i2h" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:10, 7 January 2013

OCA at 12:10, 7 January 2013

2013-01-07T12:10:19Z

←Older revision		Revision as of 12:10, 7 January 2013
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-	{{Seed}}
	[[Image:2i2h.png\|left\|200px]]		[[Image:2i2h.png\|left\|200px]]

-	<!--
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-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_2i2h\| PDB=2i2h \| SCENE= }}		{{STRUCTURE_2i2h\| PDB=2i2h \| SCENE= }}

	===NMR structure of TPC3 in TFE===		===NMR structure of TPC3 in TFE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16936029}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16936029 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_16936029}}		{{ABSTRACT_PUBMED_16936029}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA].	+	[[2i2h]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA].

	==Reference==		==Reference==
-	Structure-activity analysis of quorum-sensing signaling peptides from Streptococcus mutans., Syvitski RT, Tian XL, Sampara K, Salman A, Lee SF, Jakeman DL, Li YH, J Bacteriol. 2007 Feb;189(4):1441-50. Epub 2006 Aug 25. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~16936029 16936029]~~	+	<ref group="xtra">PMID:016936029</ref><references group="xtra"/>
	[[Category: Jakeman, D L.]]		[[Category: Jakeman, D L.]]
	[[Category: Li, Y.]]		[[Category: Li, Y.]]
	[[Category: Syvitski, R T.]]		[[Category: Syvitski, R T.]]
	[[Category: Helix]]		[[Category: Helix]]
-		+	[[Category: Signaling protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Sun Jul 27 14:40:19 2008''~~	+

OCA at 11:40, 27 July 2008

2008-07-27T11:40:23Z

←Older revision		Revision as of 11:40, 27 July 2008
Line 1:		Line 1:
-	[[Image:2i2h.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2i2h.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2i2h\| PDB=2i2h \| SCENE= }}		{{STRUCTURE_2i2h\| PDB=2i2h \| SCENE= }}

-	~~'''~~NMR structure of TPC3 in TFE~~'''~~	+	===NMR structure of TPC3 in TFE===


-	~~==Overview==~~	+	<!--
-	~~Streptococcus mutans secretes and utilizes a 21~~-~~amino~~-~~acid signaling peptide pheromone to initiate quorum sensing for genetic competence, biofilm formation, stress responses, and bacteriocin production. In~~ this ~~study~~, we designed and synthesized a series of truncated peptides and peptides with amino acid substitutions to investigate their structure-activity relationships based on the three-dimensional structures of S. mutans wild-type signaling peptide UA159sp and C-terminally truncated peptide TPC3 from mutant JH1005 defective in genetic competence. By analyzing these peptides, ~~we demonstrated that~~ the signaling peptide of S. mutans has at least two functional domains. The C-terminal structural motif consisting of a sequence of polar hydrophobic charged residues is crucial for activation of the signal transduction pathway, while the core alpha-helical structure extending from residue 5 to the ~~end of the peptide is required for receptor binding~~. ~~Peptides in which three or more residues were deleted from the C terminus did not induce genetic competence but competitively inhibited quorum sensing activated by UA159sp~~. ~~Disruption of the amphipathic alpha-helix by replacing the Phe-7~~, ~~Phe-11, or Phe-15 residue with a hydrophilic residue resulted in a significant reduction in or complete loss of~~ the ~~activity of the peptide~~. ~~In contrast to the C~~-~~terminally truncated peptides, these peptides with amino acid substitutions did not compete with UA159sp to activate quorum sensing, suggesting that disruption of the hydrophobic face of the alpha~~-helical structure results in a peptide that is not able to bind to the receptor. This study is the first study to recognize the importance of the signaling peptide C-terminal residues in streptococcal quorum sensing.	+	The line below this paragraph, {{ABSTRACT_PUBMED_16936029}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 16936029 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_16936029}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2H OCA].

	==Reference==		==Reference==
Line 24:		Line 28:
	[[Category: Syvitski, R T.]]		[[Category: Syvitski, R T.]]
	[[Category: Helix]]		[[Category: Helix]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun ~~May 4 06~~:59:48 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:40:19 2008''