2i5m - Revision history

OCA at 10:06, 30 August 2023

2023-08-30T10:06:25Z

←Older revision		Revision as of 10:06, 30 August 2023
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	<StructureSection load='2i5m' size='340' side='right'caption='[[2i5m]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2i5m' size='340' side='right'caption='[[2i5m]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i5m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"vibrio_subtilis"_ehrenberg_1835 "vibrio~~ subtilis~~" ehrenberg 1835~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I5M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i5m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I5M FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[1csq\|1csq]], [[1csp\|1csp]], [[2es2\|2es2]], [[2i5l\|2i5l]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">cspB, cspA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5m OCA], [https://pdbe.org/2i5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i5m RCSB], [https://www.ebi.ac.uk/pdbsum/2i5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i5m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5m OCA], [https://pdbe.org/2i5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i5m RCSB], [https://www.ebi.ac.uk/pdbsum/2i5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i5m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CSPB_BACSU CSPB_BACSU]] Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.	+	[https://www.uniprot.org/uniprot/CSPB_BACSU CSPB_BACSU] Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio~~ subtilis ~~ehrenberg 1835~~]]	+	[[Category: Bacillus subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Heinemann, U]]	+	[[Category: Heinemann U]]
-	[[Category: Max~~, K E.A]]~~	+	[[Category: Max KEA]]
-	~~[[Category: Beta-barrel]]~~	+
-	~~[[Category: Cold shock domain]]~~	+
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Expression regulator]]~~	+
-	~~[[Category: Gene regulation]]~~	+
-	~~[[Category: Oligonucleotide/oligosaccharide binding fold~~]]	+

OCA at 17:49, 20 October 2021

2021-10-20T17:49:38Z

←Older revision		Revision as of 17:49, 20 October 2021
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	<StructureSection load='2i5m' size='340' side='right'caption='[[2i5m]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2i5m' size='340' side='right'caption='[[2i5m]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i5m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"~~bacillus_globigii~~"~~_migula_1900~~ "~~bacillus globigii~~" ~~migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I5M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i5m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I5M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1csq\|1csq]], [[1csp\|1csp]], [[2es2\|2es2]], [[2i5l\|2i5l]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1csq\|1csq]], [[1csp\|1csp]], [[2es2\|2es2]], [[2i5l\|2i5l]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cspB, cspA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "~~Bacillus globigii~~" ~~Migula 1900~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cspB, cspA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5m OCA], [https://pdbe.org/2i5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i5m RCSB], [https://www.ebi.ac.uk/pdbsum/2i5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i5m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5m OCA], [https://pdbe.org/2i5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i5m RCSB], [https://www.ebi.ac.uk/pdbsum/2i5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i5m ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus globigii migula 1900~~]]	+	[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Heinemann, U]]		[[Category: Heinemann, U]]

OCA at 07:28, 24 March 2021

2021-03-24T07:28:26Z

←Older revision		Revision as of 07:28, 24 March 2021
Line 1:		Line 1:

	==Crystal structure of Bacillus subtilis cold shock protein CspB variant A46K S48R==		==Crystal structure of Bacillus subtilis cold shock protein CspB variant A46K S48R==
-	<StructureSection load='2i5m' size='340' side='right' caption='[[2i5m]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='2i5m' size='340' side='right'caption='[[2i5m]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i5m]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2I5M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i5m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I5M FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csq\|1csq]], [[1csp\|1csp]], [[2es2\|2es2]], [[2i5l\|2i5l]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1csq\|1csq]], [[1csp\|1csp]], [[2es2\|2es2]], [[2i5l\|2i5l]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cspB, cspA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cspB, cspA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2i5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5m OCA], [~~http~~://pdbe.org/2i5m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2i5m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2i5m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2i5m ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5m OCA], [https://pdbe.org/2i5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i5m RCSB], [https://www.ebi.ac.uk/pdbsum/2i5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i5m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CSPB_BACSU CSPB_BACSU]] Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.	+	[[https://www.uniprot.org/uniprot/CSPB_BACSU CSPB_BACSU]] Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/2i5m_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/2i5m_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus globigii migula 1900]]		[[Category: Bacillus globigii migula 1900]]
		+	[[Category: Large Structures]]
	[[Category: Heinemann, U]]		[[Category: Heinemann, U]]
	[[Category: Max, K E.A]]		[[Category: Max, K E.A]]

OCA at 08:23, 18 October 2017

2017-10-18T08:23:23Z

←Older revision		Revision as of 08:23, 18 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of Bacillus subtilis cold shock protein CspB variant A46K S48R==		==Crystal structure of Bacillus subtilis cold shock protein CspB variant A46K S48R==
	<StructureSection load='2i5m' size='340' side='right' caption='[[2i5m]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2i5m' size='340' side='right' caption='[[2i5m]], [[Resolution\|resolution]] 2.30Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csq\|1csq]], [[1csp\|1csp]], [[2es2\|2es2]], [[2i5l\|2i5l]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csq\|1csq]], [[1csp\|1csp]], [[2es2\|2es2]], [[2i5l\|2i5l]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cspB, cspA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cspB, cspA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5m OCA], [http://pdbe.org/2i5m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i5m RCSB], [http://www.ebi.ac.uk/pdbsum/2i5m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5m OCA], [http://pdbe.org/2i5m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i5m RCSB], [http://www.ebi.ac.uk/pdbsum/2i5m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i5m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 05:52, 10 February 2016

2016-02-10T05:52:05Z

←Older revision		Revision as of 05:52, 10 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i5m ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:59, 11 September 2015

2015-09-11T05:59:23Z

←Older revision		Revision as of 05:59, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2i5m' size='340' side='right' caption='[[2i5m]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2i5m' size='340' side='right' caption='[[2i5m]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i5m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I5M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i5m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I5M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csq\|1csq]], [[1csp\|1csp]], [[2es2\|2es2]], [[2i5l\|2i5l]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csq\|1csq]], [[1csp\|1csp]], [[2es2\|2es2]], [[2i5l\|2i5l]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cspB, cspA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus ~~subtilis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cspB, cspA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i5m RCSB], [http://www.ebi.ac.uk/pdbsum/2i5m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5m OCA], [http://pdbe.org/2i5m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i5m RCSB], [http://www.ebi.ac.uk/pdbsum/2i5m PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2i5m" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus ~~subtilis~~]]	+	[[Category: Bacillus globigii migula 1900]]
	[[Category: Heinemann, U]]		[[Category: Heinemann, U]]
	[[Category: Max, K E.A]]		[[Category: Max, K E.A]]

OCA at 12:15, 25 December 2014

2014-12-25T12:15:50Z

←Older revision		Revision as of 12:15, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2i5m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I5M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2i5m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I5M FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csq\|1csq]], [[1csp\|1csp]], [[2es2\|2es2]], [[2i5l\|2i5l]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csq\|1csq]], [[1csp\|1csp]], [[2es2\|2es2]], [[2i5l\|2i5l]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cspB, cspA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cspB, cspA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i5m RCSB], [http://www.ebi.ac.uk/pdbsum/2i5m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i5m RCSB], [http://www.ebi.ac.uk/pdbsum/2i5m PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CSPB_BACSU CSPB_BACSU]] Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
-	[[Category: Heinemann, U.]]	+	[[Category: Heinemann, U]]
-	[[Category: Max, K E.A.]]	+	[[Category: Max, K E.A]]
	[[Category: Beta-barrel]]		[[Category: Beta-barrel]]
	[[Category: Cold shock domain]]		[[Category: Cold shock domain]]

OCA at 09:15, 30 September 2014

2014-09-30T09:15:50Z

←Older revision		Revision as of 09:15, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2i5m.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of Bacillus subtilis cold shock protein CspB variant A46K S48R==
		+	<StructureSection load='2i5m' size='340' side='right' caption='[[2i5m]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2i5m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I5M FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csq\|1csq]], [[1csp\|1csp]], [[2es2\|2es2]], [[2i5l\|2i5l]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cspB, cspA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i5m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i5m RCSB], [http://www.ebi.ac.uk/pdbsum/2i5m PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/2i5m_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacterial cold shock proteins (Csp) are widely used as models for the experimental and computational analysis of protein stability. In a previous study, in vitro evolution was employed to identify strongly stabilizing mutations in Bs-CspB from Bacillus subtilis. The best variant found by this approach contained the mutations M1R, E3K and K65I, which raised the midpoint of thermal unfolding of Bs-CspB from 53.8 degrees C to 83.7 degrees C, and increased the Gibbs free energy of stabilization by 20.9 kJ mol(-1). Another selected variant with the two mutations A46K and S48R was stabilized by 11.1 kJ mol(-1). To elucidate the molecular basis of these stabilizations, we determined the crystal structures of these two Bs-CspB variants. The mutated residues are generally well ordered and provide additional stabilizing interactions, such as charge interactions, additional hydrogen bonds and improved side-chain packing. Several mutations improve the electrostatic interactions, either by the removal of unfavorable charges (E3K) or by compensating their destabilizing interactions (A46K, S48R). The stabilizing mutations are clustered at a contiguous surface area of Bs-CspB, which apparently is critically important for the stability of the beta-barrel structure but not well optimized in the wild-type protein.

-	~~{{STRUCTURE_2i5m\| PDB=2i5m \| SCENE= }}~~	+	Optimized variants of the cold shock protein from in vitro selection: structural basis of their high thermostability.,Max KE, Wunderlich M, Roske Y, Schmid FX, Heinemann U J Mol Biol. 2007 Jun 15;369(4):1087-97. Epub 2007 Apr 12. PMID:17481655<ref>PMID:17481655</ref>

-	~~===Crystal structure~~ of ~~Bacillus subtilis cold shock protein CspB variant A46K S48R===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17481655}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2i5m]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_subtilis Bacillus subtilis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017481655<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Heinemann, U.]]		[[Category: Heinemann, U.]]

OCA: Protected "2i5m" [edit=sysop:move=sysop]

2013-01-07T11:32:13Z

Protected "2i5m" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:32, 7 January 2013

OCA at 11:32, 7 January 2013

2013-01-07T11:32:11Z

←Older revision		Revision as of 11:32, 7 January 2013
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-	{{Seed}}
	[[Image:2i5m.png\|left\|200px]]		[[Image:2i5m.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2i5m", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2i5m\| PDB=2i5m \| SCENE= }}		{{STRUCTURE_2i5m\| PDB=2i5m \| SCENE= }}

	===Crystal structure of Bacillus subtilis cold shock protein CspB variant A46K S48R===		===Crystal structure of Bacillus subtilis cold shock protein CspB variant A46K S48R===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17481655}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17481655 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17481655}}		{{ABSTRACT_PUBMED_17481655}}

	==About this Structure==		==About this Structure==
-	~~2I5M~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA].	+	[[2i5m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5M OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17481655~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017481655</ref><references group="xtra"/>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Heinemann, U.]]		[[Category: Heinemann, U.]]
Line 31:		Line 19:
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]
	[[Category: Expression regulator]]		[[Category: Expression regulator]]
		+	[[Category: Gene regulation]]
	[[Category: Oligonucleotide/oligosaccharide binding fold]]		[[Category: Oligonucleotide/oligosaccharide binding fold]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 15:55:44 2009''