2iaq - Revision history

OCA at 10:08, 30 August 2023

2023-08-30T10:08:12Z

←Older revision		Revision as of 10:08, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='2iaq' size='340' side='right'caption='[[2iaq]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2iaq' size='340' side='right'caption='[[2iaq]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2iaq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Common_european_squid Common european squid~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IAQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2iaq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Loligo_vulgaris Loligo vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IAQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1e1a\|1e1a]], [[1pjx\|1pjx]], [[2iao\|2iao]], [[2iap\|2iap]], [[2iar\|2iar]], [[2ias\|2ias]], [[2iat\|2iat]], [[2iau\|2iau]], [[2iav\|2iav]], [[2iaw\|2iaw]], [[2iax\|2iax]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Diisopropyl-fluorophosphatase Diisopropyl-fluorophosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iaq OCA], [https://pdbe.org/2iaq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iaq RCSB], [https://www.ebi.ac.uk/pdbsum/2iaq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iaq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iaq OCA], [https://pdbe.org/2iaq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iaq RCSB], [https://www.ebi.ac.uk/pdbsum/2iaq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iaq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DFPA_LOLVU DFPA_LOLVU]] Biological function and substrate unknown. However, it is capable of acting on phosphorus anhydride bonds (such as phosphorus-halide and phosphorus-cyanide) in organophosphorus compounds (including nerve gases).<ref>PMID:15966726</ref>	+	[https://www.uniprot.org/uniprot/DFPA_LOLVU DFPA_LOLVU] Biological function and substrate unknown. However, it is capable of acting on phosphorus anhydride bonds (such as phosphorus-halide and phosphorus-cyanide) in organophosphorus compounds (including nerve gases).<ref>PMID:15966726</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Common european squid]]
-	[[Category: Diisopropyl-fluorophosphatase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fritzsch, G]]	+	[[Category: Loligo vulgaris]]
-	[[Category: Koepke, J]]	+	[[Category: Fritzsch G]]
-	[[Category: Luecke, C]]	+	[[Category: Koepke J]]
-	[[Category: Rueterjans, H]]	+	[[Category: Luecke C]]
-	[[Category: Scharff~~, E I]]~~	+	[[Category: Rueterjans H]]
-	~~[[Category: Beta-propeller]]~~	+	[[Category: Scharff EI]]
-	~~[[Category: Calcium-binding site]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phosphotriesterase~~]]	+

OCA at 17:50, 20 October 2021

2021-10-20T17:50:42Z

←Older revision		Revision as of 17:50, 20 October 2021
Line 3:		Line 3:
	<StructureSection load='2iaq' size='340' side='right'caption='[[2iaq]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2iaq' size='340' side='right'caption='[[2iaq]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2iaq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Common_european_squid Common european squid]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2IAQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2iaq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Common_european_squid Common european squid]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IAQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e1a\|1e1a]], [[1pjx\|1pjx]], [[2iao\|2iao]], [[2iap\|2iap]], [[2iar\|2iar]], [[2ias\|2ias]], [[2iat\|2iat]], [[2iau\|2iau]], [[2iav\|2iav]], [[2iaw\|2iaw]], [[2iax\|2iax]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1e1a\|1e1a]], [[1pjx\|1pjx]], [[2iao\|2iao]], [[2iap\|2iap]], [[2iar\|2iar]], [[2ias\|2ias]], [[2iat\|2iat]], [[2iau\|2iau]], [[2iav\|2iav]], [[2iaw\|2iaw]], [[2iax\|2iax]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Diisopropyl-fluorophosphatase Diisopropyl-fluorophosphatase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Diisopropyl-fluorophosphatase Diisopropyl-fluorophosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2iaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iaq OCA], [~~http~~://pdbe.org/2iaq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2iaq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2iaq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2iaq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iaq OCA], [https://pdbe.org/2iaq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iaq RCSB], [https://www.ebi.ac.uk/pdbsum/2iaq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iaq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DFPA_LOLVU DFPA_LOLVU]] Biological function and substrate unknown. However, it is capable of acting on phosphorus anhydride bonds (such as phosphorus-halide and phosphorus-cyanide) in organophosphorus compounds (including nerve gases).<ref>PMID:15966726</ref>	+	[[https://www.uniprot.org/uniprot/DFPA_LOLVU DFPA_LOLVU]] Biological function and substrate unknown. However, it is capable of acting on phosphorus anhydride bonds (such as phosphorus-halide and phosphorus-cyanide) in organophosphorus compounds (including nerve gases).<ref>PMID:15966726</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:43, 8 April 2020

2020-04-08T07:43:51Z

←Older revision		Revision as of 07:43, 8 April 2020
Line 1:		Line 1:

	==Crystal structure of squid ganglion DFPase S271A mutant==		==Crystal structure of squid ganglion DFPase S271A mutant==
-	<StructureSection load='2iaq' size='340' side='right' caption='[[2iaq]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='2iaq' size='340' side='right'caption='[[2iaq]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2iaq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_european_squid Common european squid]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2IAQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2iaq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_european_squid Common european squid]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2IAQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e1a\|1e1a]], [[1pjx\|1pjx]], [[2iao\|2iao]], [[2iap\|2iap]], [[2iar\|2iar]], [[2ias\|2ias]], [[2iat\|2iat]], [[2iau\|2iau]], [[2iav\|2iav]], [[2iaw\|2iaw]], [[2iax\|2iax]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e1a\|1e1a]], [[1pjx\|1pjx]], [[2iao\|2iao]], [[2iap\|2iap]], [[2iar\|2iar]], [[2ias\|2ias]], [[2iat\|2iat]], [[2iau\|2iau]], [[2iav\|2iav]], [[2iaw\|2iaw]], [[2iax\|2iax]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diisopropyl-fluorophosphatase Diisopropyl-fluorophosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diisopropyl-fluorophosphatase Diisopropyl-fluorophosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2iaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iaq OCA], [http://pdbe.org/2iaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iaq RCSB], [http://www.ebi.ac.uk/pdbsum/2iaq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2iaq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2iaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iaq OCA], [http://pdbe.org/2iaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iaq RCSB], [http://www.ebi.ac.uk/pdbsum/2iaq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2iaq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 36:		Line 36:
	[[Category: Common european squid]]		[[Category: Common european squid]]
	[[Category: Diisopropyl-fluorophosphatase]]		[[Category: Diisopropyl-fluorophosphatase]]
		+	[[Category: Large Structures]]
	[[Category: Fritzsch, G]]		[[Category: Fritzsch, G]]
	[[Category: Koepke, J]]		[[Category: Koepke, J]]

OCA at 07:56, 4 July 2018

2018-07-04T07:56:44Z

←Older revision		Revision as of 07:56, 4 July 2018
Line 1:		Line 1:
		+
	==Crystal structure of squid ganglion DFPase S271A mutant==		==Crystal structure of squid ganglion DFPase S271A mutant==
	<StructureSection load='2iaq' size='340' side='right' caption='[[2iaq]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2iaq' size='340' side='right' caption='[[2iaq]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e1a\|1e1a]], [[1pjx\|1pjx]], [[2iao\|2iao]], [[2iap\|2iap]], [[2iar\|2iar]], [[2ias\|2ias]], [[2iat\|2iat]], [[2iau\|2iau]], [[2iav\|2iav]], [[2iaw\|2iaw]], [[2iax\|2iax]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e1a\|1e1a]], [[1pjx\|1pjx]], [[2iao\|2iao]], [[2iap\|2iap]], [[2iar\|2iar]], [[2ias\|2ias]], [[2iat\|2iat]], [[2iau\|2iau]], [[2iav\|2iav]], [[2iaw\|2iaw]], [[2iax\|2iax]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diisopropyl-fluorophosphatase Diisopropyl-fluorophosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diisopropyl-fluorophosphatase Diisopropyl-fluorophosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iaq OCA], [http://pdbe.org/2iaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iaq RCSB], [http://www.ebi.ac.uk/pdbsum/2iaq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iaq OCA], [http://pdbe.org/2iaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iaq RCSB], [http://www.ebi.ac.uk/pdbsum/2iaq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2iaq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/2iaq_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/2iaq_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 16:08, 8 February 2016

2016-02-08T16:08:47Z

←Older revision		Revision as of 16:08, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iaq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:15, 10 September 2015

2015-09-10T13:15:11Z

←Older revision		Revision as of 13:15, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2iaq' size='340' side='right' caption='[[2iaq]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2iaq' size='340' side='right' caption='[[2iaq]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2iaq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Loligo_vulgaris Loligo vulgaris~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IAQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2iaq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_european_squid Common european squid]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IAQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e1a\|1e1a]], [[1pjx\|1pjx]], [[2iao\|2iao]], [[2iap\|2iap]], [[2iar\|2iar]], [[2ias\|2ias]], [[2iat\|2iat]], [[2iau\|2iau]], [[2iav\|2iav]], [[2iaw\|2iaw]], [[2iax\|2iax]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e1a\|1e1a]], [[1pjx\|1pjx]], [[2iao\|2iao]], [[2iap\|2iap]], [[2iar\|2iar]], [[2ias\|2ias]], [[2iat\|2iat]], [[2iau\|2iau]], [[2iav\|2iav]], [[2iaw\|2iaw]], [[2iax\|2iax]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diisopropyl-fluorophosphatase Diisopropyl-fluorophosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diisopropyl-fluorophosphatase Diisopropyl-fluorophosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iaq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iaq RCSB], [http://www.ebi.ac.uk/pdbsum/2iaq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iaq OCA], [http://pdbe.org/2iaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iaq RCSB], [http://www.ebi.ac.uk/pdbsum/2iaq PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2iaq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Common european squid]]
	[[Category: Diisopropyl-fluorophosphatase]]		[[Category: Diisopropyl-fluorophosphatase]]
-	[[Category: Loligo vulgaris]]
	[[Category: Fritzsch, G]]		[[Category: Fritzsch, G]]
	[[Category: Koepke, J]]		[[Category: Koepke, J]]

OCA at 00:32, 25 December 2014

2014-12-25T00:32:27Z

←Older revision		Revision as of 00:32, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2iaq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Loligo_vulgaris Loligo vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IAQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2iaq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Loligo_vulgaris Loligo vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IAQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e1a\|1e1a]], [[1pjx\|1pjx]], [[2iao\|2iao]], [[2iap\|2iap]], [[2iar\|2iar]], [[2ias\|2ias]], [[2iat\|2iat]], [[2iau\|2iau]], [[2iav\|2iav]], [[2iaw\|2iaw]], [[2iax\|2iax]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e1a\|1e1a]], [[1pjx\|1pjx]], [[2iao\|2iao]], [[2iap\|2iap]], [[2iar\|2iar]], [[2ias\|2ias]], [[2iat\|2iat]], [[2iau\|2iau]], [[2iav\|2iav]], [[2iaw\|2iaw]], [[2iax\|2iax]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diisopropyl-fluorophosphatase Diisopropyl-fluorophosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diisopropyl-fluorophosphatase Diisopropyl-fluorophosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iaq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iaq RCSB], [http://www.ebi.ac.uk/pdbsum/2iaq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iaq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iaq RCSB], [http://www.ebi.ac.uk/pdbsum/2iaq PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DFPA_LOLVU DFPA_LOLVU]] Biological function and substrate unknown. However, it is capable of acting on phosphorus anhydride bonds (such as phosphorus-halide and phosphorus-cyanide) in organophosphorus compounds (including nerve gases).<ref>PMID:15966726</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	[[Category: Diisopropyl-fluorophosphatase]]		[[Category: Diisopropyl-fluorophosphatase]]
	[[Category: Loligo vulgaris]]		[[Category: Loligo vulgaris]]
-	[[Category: Fritzsch, G.]]	+	[[Category: Fritzsch, G]]
-	[[Category: Koepke, J.]]	+	[[Category: Koepke, J]]
-	[[Category: Luecke, C.]]	+	[[Category: Luecke, C]]
-	[[Category: Rueterjans, H.]]	+	[[Category: Rueterjans, H]]
-	[[Category: Scharff, E I.]]	+	[[Category: Scharff, E I]]
	[[Category: Beta-propeller]]		[[Category: Beta-propeller]]
	[[Category: Calcium-binding site]]		[[Category: Calcium-binding site]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Phosphotriesterase]]		[[Category: Phosphotriesterase]]

OCA at 07:44, 30 September 2014

2014-09-30T07:44:40Z

←Older revision		Revision as of 07:44, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2iaq.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of squid ganglion DFPase S271A mutant==
		+	<StructureSection load='2iaq' size='340' side='right' caption='[[2iaq]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2iaq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Loligo_vulgaris Loligo vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IAQ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e1a\|1e1a]], [[1pjx\|1pjx]], [[2iao\|2iao]], [[2iap\|2iap]], [[2iar\|2iar]], [[2ias\|2ias]], [[2iat\|2iat]], [[2iau\|2iau]], [[2iav\|2iav]], [[2iaw\|2iaw]], [[2iax\|2iax]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diisopropyl-fluorophosphatase Diisopropyl-fluorophosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.2 3.1.8.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iaq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iaq RCSB], [http://www.ebi.ac.uk/pdbsum/2iaq PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/2iaq_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Phosphotriesterases (PTE) are enzymes capable of detoxifying organophosphate-based chemical warfare agents by hydrolysis. One subclass of these enzymes comprises the family of diisopropylfluorophosphatases (DFPases). The DFPase reported here was originally isolated from squid head ganglion of Loligo vulgaris and can be characterized as squid-type DFPase. It is capable of hydrolyzing the organophosphates diisopropylfluorophosphate, soman, sarin, tabun, and cyclosarin. RESULTS: Crystals were grown of both the native and the selenomethionine-labeled enzyme. The X-ray crystal structure of the DFPase from Loligo vulgaris has been solved by MAD phasing and refined to a crystallographic R value of 17.6% at a final resolution of 1.8 A. Using site-directed mutagenesis, we have structurally and functionally characterized essential residues in the active site of the enzyme. CONCLUSIONS: The crystal structure of the DFPase from Loligo vulgaris is the first example of a structural characterization of a squid-type DFPase and the second crystal structure of a PTE determined to date. Therefore, it may serve as a structural model for squid-type DFPases in general. The overall structure of this protein represents a six-fold beta propeller with two calcium ions bound in a central water-filled tunnel. The consensus motif found in the blades of this beta propeller has not yet been observed in other beta propeller structures. Based on the results obtained from mutants of active-site residues, a mechanistic model for the DFP hydrolysis has been developed.

-	~~{{STRUCTURE_2iaq\| PDB=2iaq \| SCENE= }}~~	+	Crystal structure of diisopropylfluorophosphatase from Loligo vulgaris.,Scharff EI, Koepke J, Fritzsch G, Lucke C, Ruterjans H Structure. 2001 Jun;9(6):493-502. PMID:11435114<ref>PMID:11435114</ref>

-	~~===Crystal structure~~ of ~~squid ganglion DFPase S271A mutant===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11435114}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2iaq]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Loligo_vulgaris Loligo vulgaris]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011435114<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Diisopropyl-fluorophosphatase]]		[[Category: Diisopropyl-fluorophosphatase]]
	[[Category: Loligo vulgaris]]		[[Category: Loligo vulgaris]]

OCA: Protected "2iaq" [edit=sysop:move=sysop]

2013-01-07T12:53:02Z

Protected "2iaq" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:53, 7 January 2013

OCA at 12:52, 7 January 2013

2013-01-07T12:52:58Z

←Older revision		Revision as of 12:52, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2iaq.png\|left\|200px]]		[[Image:2iaq.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2iaq", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2iaq\| PDB=2iaq \| SCENE= }}		{{STRUCTURE_2iaq\| PDB=2iaq \| SCENE= }}

	===Crystal structure of squid ganglion DFPase S271A mutant===		===Crystal structure of squid ganglion DFPase S271A mutant===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11435114}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11435114 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11435114}}		{{ABSTRACT_PUBMED_11435114}}

	==About this Structure==		==About this Structure==
-	~~2IAQ~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Loligo_vulgaris Loligo vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA].	+	[[2iaq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Loligo_vulgaris Loligo vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAQ OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11435114~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011435114</ref><references group="xtra"/>
	[[Category: Diisopropyl-fluorophosphatase]]		[[Category: Diisopropyl-fluorophosphatase]]
	[[Category: Loligo vulgaris]]		[[Category: Loligo vulgaris]]
Line 33:		Line 21:
	[[Category: Beta-propeller]]		[[Category: Beta-propeller]]
	[[Category: Calcium-binding site]]		[[Category: Calcium-binding site]]
		+	[[Category: Hydrolase]]
	[[Category: Phosphotriesterase]]		[[Category: Phosphotriesterase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:19:52 2009''