2ifz - Revision history

OCA at 12:37, 10 January 2024

2024-01-10T12:37:46Z

←Older revision		Revision as of 12:37, 10 January 2024
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	==Lys6 Variant of ImI Conotoxin==		==Lys6 Variant of ImI Conotoxin==
-	<StructureSection load='2ifz' size='340' side='right'caption='[[2ifz~~]], [[NMR_Ensembles_of_Models \| 15 NMR models~~]]' scene=''>	+	<StructureSection load='2ifz' size='340' side='right'caption='[[2ifz]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ifz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ifz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IFZ FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=NH2:AMINO+GROUP'>NH2</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2ifi\|2ifi]], [[2ifj\|2ifj]], [[2igu\|2igu]], [[2ih6\|2ih6]], [[2ih7\|2ih7]], [[2iha\|2iha]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ifz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ifz OCA], [https://pdbe.org/2ifz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ifz RCSB], [https://www.ebi.ac.uk/pdbsum/2ifz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ifz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ifz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ifz OCA], [https://pdbe.org/2ifz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ifz RCSB], [https://www.ebi.ac.uk/pdbsum/2ifz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ifz ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Kang, T S~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Kini, R M~~]]	+	[[Category: Kang TS]]
-	[[Category: ~~Conotoxin]]~~	+	[[Category: Kini RM]]
-	~~[[Category: Disulfide linkage]]~~	+
-	~~[[Category: Ribbon conformation]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 10:55, 6 April 2022

2022-04-06T10:55:19Z

←Older revision		Revision as of 10:55, 6 April 2022
Line 3:		Line 3:
	<StructureSection load='2ifz' size='340' side='right'caption='[[2ifz]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>		<StructureSection load='2ifz' size='340' side='right'caption='[[2ifz]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ifz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2IFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ifz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IFZ FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ifi\|2ifi]], [[2ifj\|2ifj]], [[2igu\|2igu]], [[2ih6\|2ih6]], [[2ih7\|2ih7]], [[2iha\|2iha]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ifi\|2ifi]], [[2ifj\|2ifj]], [[2igu\|2igu]], [[2ih6\|2ih6]], [[2ih7\|2ih7]], [[2iha\|2iha]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2ifz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ifz OCA], [~~http~~://pdbe.org/2ifz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ifz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ifz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ifz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ifz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ifz OCA], [https://pdbe.org/2ifz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ifz RCSB], [https://www.ebi.ac.uk/pdbsum/2ifz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ifz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:45, 8 April 2020

2020-04-08T07:45:22Z

←Older revision		Revision as of 07:45, 8 April 2020
Line 1:		Line 1:

	==Lys6 Variant of ImI Conotoxin==		==Lys6 Variant of ImI Conotoxin==
-	<StructureSection load='2ifz' size='340' side='right' caption='[[2ifz]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>	+	<StructureSection load='2ifz' size='340' side='right'caption='[[2ifz]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ifz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2IFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ifz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2IFZ FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ifi\|2ifi]], [[2ifj\|2ifj]], [[2igu\|2igu]], [[2ih6\|2ih6]], [[2ih7\|2ih7]], [[2iha\|2iha]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ifi\|2ifi]], [[2ifj\|2ifj]], [[2igu\|2igu]], [[2ih6\|2ih6]], [[2ih7\|2ih7]], [[2iha\|2iha]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ifz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ifz OCA], [http://pdbe.org/2ifz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ifz RCSB], [http://www.ebi.ac.uk/pdbsum/2ifz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ifz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2ifz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ifz OCA], [http://pdbe.org/2ifz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ifz RCSB], [http://www.ebi.ac.uk/pdbsum/2ifz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ifz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Kang, T S]]		[[Category: Kang, T S]]
	[[Category: Kini, R M]]		[[Category: Kini, R M]]

OCA at 08:13, 4 July 2018

2018-07-04T08:13:46Z

←Older revision		Revision as of 08:13, 4 July 2018
Line 1:		Line 1:
		+
	==Lys6 Variant of ImI Conotoxin==		==Lys6 Variant of ImI Conotoxin==
	<StructureSection load='2ifz' size='340' side='right' caption='[[2ifz]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>		<StructureSection load='2ifz' size='340' side='right' caption='[[2ifz]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ifi\|2ifi]], [[2ifj\|2ifj]], [[2igu\|2igu]], [[2ih6\|2ih6]], [[2ih7\|2ih7]], [[2iha\|2iha]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ifi\|2ifi]], [[2ifj\|2ifj]], [[2igu\|2igu]], [[2ih6\|2ih6]], [[2ih7\|2ih7]], [[2iha\|2iha]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ifz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ifz OCA], [http://pdbe.org/2ifz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ifz RCSB], [http://www.ebi.ac.uk/pdbsum/2ifz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ifz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ifz OCA], [http://pdbe.org/2ifz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ifz RCSB], [http://www.ebi.ac.uk/pdbsum/2ifz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ifz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:04, 11 September 2015

2015-09-11T01:04:16Z

←Older revision		Revision as of 01:04, 11 September 2015
Line 5:		Line 5:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ifi\|2ifi]], [[2ifj\|2ifj]], [[2igu\|2igu]], [[2ih6\|2ih6]], [[2ih7\|2ih7]], [[2iha\|2iha]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ifi\|2ifi]], [[2ifj\|2ifj]], [[2igu\|2igu]], [[2ih6\|2ih6]], [[2ih7\|2ih7]], [[2iha\|2iha]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ifz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ifz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ifz RCSB], [http://www.ebi.ac.uk/pdbsum/2ifz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ifz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ifz OCA], [http://pdbe.org/2ifz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ifz RCSB], [http://www.ebi.ac.uk/pdbsum/2ifz PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ifz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:07, 16 January 2015

2015-01-16T09:07:49Z

←Older revision		Revision as of 09:07, 16 January 2015
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Kang, T S.]]	+	[[Category: Kang, T S]]
-	[[Category: Kini, R M.]]	+	[[Category: Kini, R M]]
	[[Category: Conotoxin]]		[[Category: Conotoxin]]
	[[Category: Disulfide linkage]]		[[Category: Disulfide linkage]]
	[[Category: Ribbon conformation]]		[[Category: Ribbon conformation]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 15:39, 12 October 2014

2014-10-12T15:39:55Z

←Older revision		Revision as of 15:39, 12 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2ifz.~~png~~\|~~left~~\|~~200px~~]]	+	==Lys6 Variant of ImI Conotoxin==
		+	<StructureSection load='2ifz' size='340' side='right' caption='[[2ifz]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ifz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IFZ FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ifi\|2ifi]], [[2ifj\|2ifj]], [[2igu\|2igu]], [[2ih6\|2ih6]], [[2ih7\|2ih7]], [[2iha\|2iha]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ifz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ifz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ifz RCSB], [http://www.ebi.ac.uk/pdbsum/2ifz PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Alpha-conotoxins isolated from Conus venoms contain 11-19 residues and preferentially fold into the globular conformation that possesses a specific disulfide pairing pattern (C1-3, C2-4). We and others isolated a new family of chi-conotoxins (also called lambda conotoxins) with the conserved cysteine framework of alpha-conotoxins but with alternative disulfide pairing (C1-4, C2-3) resulting in the ribbon conformation. In both families, disulfide pairing and hence folding are important for their biological potency. By comparing the structural differences, we identified potential structural determinants responsible for the folding tendencies of these conotoxins. We examined the role of conserved proline in the first intercysteine loop and the conserved C-terminal amide on folding patterns of synthetic analogues of ImI conotoxin by comparing the isoforms with the regiospecifically synthesized conformers. Deamidation at the C-terminus and substitution of proline in the first intercysteine loop switch the folding pattern from the globular form of alpha-conotoxins to the ribbon form of chi/lambda-conotoxins. The findings are corroborated by reciprocal folding of CMrVIA chi/lambda-conotoxins. Substitution of Lys-6 from the first intercysteine loop of CMrVIA conotoxin with proline, as well as the inclusion of an amidated C-terminal shifted the folding preference of CMrVIA conotoxin from its native ribbon conformation toward the globular conformation. Binding assays of ImI conotoxin analogues with Aplysia and Bulinus acetylcholine binding protein indicate that both these substitutions and their consequent conformational change substantially impact the binding affinity of ImI conotoxin. These results strongly indicate that the first intercysteine loop proline and C-terminal amidation act as conformational switches in alpha- and chi/lambda-conotoxins.

-	~~{{STRUCTURE_2ifz\| PDB=2ifz \| SCENE= }}~~	+	Protein folding determinants: structural features determining alternative disulfide pairing in alpha- and chi/lambda-conotoxins.,Kang TS, Radic Z, Talley TT, Jois SD, Taylor P, Kini RM Biochemistry. 2007 Mar 20;46(11):3338-55. Epub 2007 Feb 22. PMID:17315952<ref>PMID:17315952</ref>

-	~~===Lys6 Variant~~ of ~~ImI Conotoxin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17315952}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2ifz]] is a 1 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2IFZ OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017315952<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Kang, T S.]]		[[Category: Kang, T S.]]
	[[Category: Kini, R M.]]		[[Category: Kini, R M.]]

OCA: Protected "2ifz" [edit=sysop:move=sysop]

2013-01-07T12:27:19Z

Protected "2ifz" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:27, 7 January 2013

OCA at 12:27, 7 January 2013

2013-01-07T12:27:17Z

←Older revision		Revision as of 12:27, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2ifz.png\|left\|200px]]		[[Image:2ifz.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2ifz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2ifz\| PDB=2ifz \| SCENE= }}		{{STRUCTURE_2ifz\| PDB=2ifz \| SCENE= }}

	===Lys6 Variant of ImI Conotoxin===		===Lys6 Variant of ImI Conotoxin===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17315952}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17315952 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17315952}}		{{ABSTRACT_PUBMED_17315952}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFZ OCA].	+	[[2ifz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFZ OCA].

	==Reference==		==Reference==
-	Protein folding determinants: structural features determining alternative disulfide pairing in alpha- and chi/lambda-conotoxins., Kang TS, Radic Z, Talley TT, Jois SD, Taylor P, Kini RM, Biochemistry. 2007 Mar 20;46(11):3338-55. Epub 2007 Feb 22. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~17315952 17315952]~~	+	<ref group="xtra">PMID:017315952</ref><references group="xtra"/>
	[[Category: Kang, T S.]]		[[Category: Kang, T S.]]
	[[Category: Kini, R M.]]		[[Category: Kini, R M.]]
	[[Category: Conotoxin]]		[[Category: Conotoxin]]
	[[Category: Disulfide linkage]]		[[Category: Disulfide linkage]]
-	[[Category: Nmr]]
	[[Category: Ribbon conformation]]		[[Category: Ribbon conformation]]
-		+	[[Category: Toxin]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Jul 28 16:10:44 2008''~~	+

OCA at 13:10, 28 July 2008

2008-07-28T13:10:47Z

←Older revision		Revision as of 13:10, 28 July 2008
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-	[[Image:2ifz.~~jpg~~\|left\|200px]]	+	{{Seed}}
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	{{STRUCTURE_2ifz\| PDB=2ifz \| SCENE= }}		{{STRUCTURE_2ifz\| PDB=2ifz \| SCENE= }}

-	~~'''~~Lys6 Variant of ImI Conotoxin~~'''~~	+	===Lys6 Variant of ImI Conotoxin===


-	~~==Overview==~~	+	<!--
-	~~Alpha~~-~~conotoxins isolated from Conus venoms contain 11~~-~~19 residues and preferentially fold into the globular conformation that possesses a specific disulfide pairing pattern (C1-3~~, ~~C2-4). We and others isolated a new family of chi-conotoxins (also called lambda conotoxins) with the conserved cysteine framework of alpha-conotoxins but with alternative disulfide pairing (C1-4~~, ~~C2-3) resulting in~~ the ~~ribbon conformation. In both families, disulfide pairing and hence folding are important for their biological potency. By comparing~~ the structural differences, we identified potential structural determinants responsible for the folding tendencies of these conotoxins. We examined the role of conserved proline in the first intercysteine loop and the conserved C-terminal amide on ~~folding patterns of synthetic analogues of ImI conotoxin by comparing the isoforms with the regiospecifically synthesized conformers. Deamidation~~ at ~~the C-terminus and substitution of proline in the first intercysteine loop switch the folding pattern from the globular form of alpha-conotoxins to the ribbon form of chi~~/~~lambda-conotoxins. The findings are corroborated by reciprocal folding of CMrVIA chi~~/~~lambda-conotoxins~~. ~~Substitution of Lys-6 from the first intercysteine loop of CMrVIA conotoxin with proline~~, ~~as well as~~ the ~~inclusion of an amidated C-terminal shifted the folding preference of CMrVIA conotoxin from its native ribbon conformation toward the globular conformation~~. Binding assays of ImI conotoxin analogues with Aplysia and Bulinus acetylcholine binding protein indicate that both these substitutions and their consequent conformational change substantially impact the binding affinity of ImI conotoxin. These results strongly indicate that the first intercysteine loop proline and C-~~terminal amidation act as conformational switches in alpha~~- ~~and chi/lambda-conotoxins.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_17315952}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17315952 is the PubMed ID number.
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		+	{{ABSTRACT_PUBMED_17315952}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFZ OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFZ OCA].

	==Reference==		==Reference==
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	[[Category: Nmr]]		[[Category: Nmr]]
	[[Category: Ribbon conformation]]		[[Category: Ribbon conformation]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 07~~:27:40 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:10:44 2008''