2inn - Revision history

OCA at 11:19, 31 March 2021

2021-03-31T11:19:26Z

←Older revision		Revision as of 11:19, 31 March 2021
Line 1:		Line 1:

	==Structure of the Phenol Hydroxyalse-Regulatory Protein Complex==		==Structure of the Phenol Hydroxyalse-Regulatory Protein Complex==
-	<StructureSection load='2inn' size='340' side='right' caption='[[2inn]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='2inn' size='340' side='right'caption='[[2inn]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2inn]] is a 7 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"achromobacter_sewerinii"_bergey_et_al._1923 "achromobacter sewerinii" bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2INN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2inn]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/"achromobacter_sewerinii"_bergey_et_al._1923 "achromobacter sewerinii" bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2INN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phN ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phL ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phO ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phM ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phN ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phO ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phM ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2inn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2inn OCA], [~~http~~://pdbe.org/2inn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2inn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2inn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2inn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2inn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2inn OCA], [https://pdbe.org/2inn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2inn RCSB], [https://www.ebi.ac.uk/pdbsum/2inn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2inn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/in/2inn_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/in/2inn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Achromobacter sewerinii bergey et al. 1923]]		[[Category: Achromobacter sewerinii bergey et al. 1923]]
		+	[[Category: Large Structures]]
	[[Category: Dunten, P W]]		[[Category: Dunten, P W]]
	[[Category: Lippard, S J]]		[[Category: Lippard, S J]]

OCA at 08:33, 18 October 2017

2017-10-18T08:33:04Z

←Older revision		Revision as of 08:33, 18 October 2017
Line 1:		Line 1:
		+
	==Structure of the Phenol Hydroxyalse-Regulatory Protein Complex==		==Structure of the Phenol Hydroxyalse-Regulatory Protein Complex==
	<StructureSection load='2inn' size='340' side='right' caption='[[2inn]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2inn' size='340' side='right' caption='[[2inn]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2inn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2inn OCA], [http://pdbe.org/2inn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2inn RCSB], [http://www.ebi.ac.uk/pdbsum/2inn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2inn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2inn OCA], [http://pdbe.org/2inn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2inn RCSB], [http://www.ebi.ac.uk/pdbsum/2inn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2inn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2inn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:32, 11 September 2015

2015-09-11T09:32:02Z

←Older revision		Revision as of 09:32, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2inn' size='340' side='right' caption='[[2inn]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2inn' size='340' side='right' caption='[[2inn]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2inn]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_stutzeri Pseudomonas stutzeri~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2INN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2inn]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_sewerinii"_bergey_et_al._1923 "achromobacter sewerinii" bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2INN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 ~~Pseudomonas stutzeri~~]), phL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 ~~Pseudomonas stutzeri~~]), phO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 ~~Pseudomonas stutzeri~~]), phM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 ~~Pseudomonas stutzeri~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923]), phM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2inn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2inn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2inn RCSB], [http://www.ebi.ac.uk/pdbsum/2inn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2inn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2inn OCA], [http://pdbe.org/2inn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2inn RCSB], [http://www.ebi.ac.uk/pdbsum/2inn PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2inn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseudomonas stutzeri~~]]	+	[[Category: Achromobacter sewerinii bergey et al. 1923]]
	[[Category: Dunten, P W]]		[[Category: Dunten, P W]]
	[[Category: Lippard, S J]]		[[Category: Lippard, S J]]

OCA at 08:39, 16 January 2015

2015-01-16T08:39:28Z

←Older revision		Revision as of 08:39, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2inn]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2INN FirstGlance]. <br>		<table><tr><td colspan='2'>[[2inn]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2INN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), phL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), phO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), phM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), phL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), phO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), phM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2inn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2inn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2inn RCSB], [http://www.ebi.ac.uk/pdbsum/2inn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2inn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2inn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2inn RCSB], [http://www.ebi.ac.uk/pdbsum/2inn PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Pseudomonas stutzeri]]		[[Category: Pseudomonas stutzeri]]
-	[[Category: Dunten, P W.]]	+	[[Category: Dunten, P W]]
-	[[Category: Lippard, S J.]]	+	[[Category: Lippard, S J]]
-	[[Category: McCormick, M S.]]	+	[[Category: McCormick, M S]]
-	[[Category: Sazinsky, M H.]]	+	[[Category: Sazinsky, M H]]
	[[Category: Complex]]		[[Category: Complex]]
	[[Category: Diiron]]		[[Category: Diiron]]

OCA at 10:13, 30 September 2014

2014-09-30T10:13:23Z

←Older revision		Revision as of 10:13, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2inn.~~png~~\|~~left~~\|200px]]	+	==Structure of the Phenol Hydroxyalse-Regulatory Protein Complex==
		+	<StructureSection load='2inn' size='340' side='right' caption='[[2inn]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2inn]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2INN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">phN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), phL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), phO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), phM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2inn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2inn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2inn RCSB], [http://www.ebi.ac.uk/pdbsum/2inn PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/in/2inn_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phenol hydroxylase (PH) belongs to a family of bacterial multicomponent monooxygenases (BMMs) with carboxylate-bridged diiron active sites. Included are toluene/o-xylene (ToMO) and soluble methane (sMMO) monooxygenase. PH hydroxylates aromatic compounds, but unlike sMMO, it cannot oxidize alkanes despite having a similar dinuclear iron active site. Important for activity is formation of a complex between the hydroxylase and a regulatory protein component. To address how structural features of BMM hydroxylases and their component complexes may facilitate the catalytic mechanism and choice of substrate, we determined X-ray structures of native and SeMet forms of the PH hydroxylase (PHH) in complex with its regulatory protein (PHM) to 2.3 A resolution. PHM binds in a canyon on one side of the (alphabetagamma)2 PHH dimer, contacting alpha-subunit helices A, E, and F approximately 12 A above the diiron core. The structure of the dinuclear iron center in PHH resembles that of mixed-valent MMOH, suggesting an Fe(II)Fe(III) oxidation state. Helix E, which comprises part of the iron-coordinating four-helix bundle, has more pi-helical character than analogous E helices in MMOH and ToMOH lacking a bound regulatory protein. Consequently, conserved active site Thr and Asn residues translocate to the protein surface, and an approximately 6 A pore opens through the four-helix bundle. Of likely functional significance is a specific hydrogen bond formed between this Asn residue and a conserved Ser side chain on PHM. The PHM protein covers a putative docking site on PHH for the PH reductase, which transfers electrons to the PHH diiron center prior to O2 activation, suggesting that the regulatory component may function to block undesired reduction of oxygenated intermediates during the catalytic cycle. A series of hydrophobic cavities through the PHH alpha-subunit, analogous to those in MMOH, may facilitate movement of the substrate to and/or product from the active site pocket. Comparisons between the ToMOH and PHH structures provide insights into their substrate regiospecificities.

-	~~{{STRUCTURE_2inn\| PDB=2inn \| SCENE= }}~~	+	X-ray structure of a hydroxylase-regulatory protein complex from a hydrocarbon-oxidizing multicomponent monooxygenase, Pseudomonas sp. OX1 phenol hydroxylase.,Sazinsky MH, Dunten PW, McCormick MS, DiDonato A, Lippard SJ Biochemistry. 2006 Dec 26;45(51):15392-404. Epub 2006 Dec 2. PMID:17176061<ref>PMID:17176061</ref>

-	~~===Structure~~ of the ~~Phenol Hydroxyalse-Regulatory Protein Complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17176061}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2inn]] is a 7 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2INN OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017176061<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Pseudomonas stutzeri]]		[[Category: Pseudomonas stutzeri]]
	[[Category: Dunten, P W.]]		[[Category: Dunten, P W.]]

OCA: Protected "2inn" [edit=sysop:move=sysop]

2013-01-07T14:41:23Z

Protected "2inn" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:41, 7 January 2013

OCA at 14:41, 7 January 2013

2013-01-07T14:41:20Z

←Older revision		Revision as of 14:41, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2inn.png\|left\|200px]]		[[Image:2inn.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2inn", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2inn\| PDB=2inn \| SCENE= }}		{{STRUCTURE_2inn\| PDB=2inn \| SCENE= }}

	===Structure of the Phenol Hydroxyalse-Regulatory Protein Complex===		===Structure of the Phenol Hydroxyalse-Regulatory Protein Complex===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17176061}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17176061 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17176061}}		{{ABSTRACT_PUBMED_17176061}}

	==About this Structure==		==About this Structure==
-	~~2INN~~ is a 7 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INN OCA].	+	[[2inn]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INN OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17176061~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017176061</ref><references group="xtra"/>
	[[Category: Pseudomonas stutzeri]]		[[Category: Pseudomonas stutzeri]]
	[[Category: Dunten, P W.]]		[[Category: Dunten, P W.]]
Line 33:		Line 21:
	[[Category: Four-helix bundle]]		[[Category: Four-helix bundle]]
	[[Category: Hydroxylase]]		[[Category: Hydroxylase]]
		+	[[Category: Oxidoreductase]]
	[[Category: Phenol]]		[[Category: Phenol]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:48:02 2009''

OCA at 16:48, 17 February 2009

2009-02-17T16:48:03Z

←Older revision		Revision as of 16:48, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2INN is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INN OCA].	+	2INN is a 7 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INN OCA].

	==Reference==		==Reference==
-	X-ray structure of a hydroxylase-regulatory protein complex from a hydrocarbon-oxidizing multicomponent monooxygenase, Pseudomonas sp. OX1 phenol hydroxylase., Sazinsky MH, Dunten PW, McCormick MS, DiDonato A, Lippard SJ, Biochemistry. 2006 Dec 26;45(51):15392-404. Epub 2006 Dec 2. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17176061 17176061]~~	+	<ref group="xtra">PMID:17176061</ref><references group="xtra"/>
-	~~[[Category: Protein complex]]~~	+
	[[Category: Pseudomonas stutzeri]]		[[Category: Pseudomonas stutzeri]]
	[[Category: Dunten, P W.]]		[[Category: Dunten, P W.]]
Line 36:		Line 35:
	[[Category: Phenol]]		[[Category: Phenol]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 02~~:51:~~11 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:48:02 2009''

OCA at 23:51, 28 July 2008

2008-07-28T23:51:17Z

←Older revision		Revision as of 23:51, 28 July 2008
Line 1:		Line 1:
-	[[Image:2inn.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2inn.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2inn\| PDB=2inn \| SCENE= }}		{{STRUCTURE_2inn\| PDB=2inn \| SCENE= }}

-	~~'''~~Structure of the Phenol Hydroxyalse-Regulatory Protein Complex~~'''~~	+	===Structure of the Phenol Hydroxyalse-Regulatory Protein Complex===


-	~~==Overview==~~	+	<!--
-	~~Phenol hydroxylase (PH) belongs to a family of bacterial multicomponent monooxygenases (BMMs) with carboxylate~~-~~bridged diiron active sites. Included are toluene/o~~-~~xylene (ToMO) and soluble methane (sMMO) monooxygenase. PH hydroxylates aromatic compounds~~, ~~but unlike sMMO~~, ~~it cannot oxidize alkanes despite having a similar dinuclear iron active site. Important for activity is formation of a complex between~~ the ~~hydroxylase and a regulatory protein component. To address how structural features of BMM hydroxylases and their component complexes may facilitate~~ the ~~catalytic mechanism and choice of substrate, we determined X-ray structures of native and SeMet forms of the PH hydroxylase~~ (~~PHH) in complex with its regulatory protein (PHM) to 2~~.~~3 A resolution~~. ~~PHM binds in a canyon on one side of the (alphabetagamma~~)~~2 PHH dimer~~, ~~contacting alpha-subunit helices A, E, and F approximately 12 A above~~ the ~~diiron core~~. ~~The structure of the dinuclear iron center in PHH resembles that of mixed~~-~~valent MMOH, suggesting an Fe(II)Fe(III) oxidation state. Helix E, which comprises part of the iron~~-coordinating four-helix bundle, has more pi-helical character than analogous E helices in MMOH and ToMOH lacking a bound regulatory protein. Consequently, conserved active site Thr and Asn residues translocate to the protein surface, and an approximately 6 A pore opens through the four-helix bundle. Of likely functional significance is a specific hydrogen bond formed between this Asn residue and a conserved Ser side chain on PHM. The PHM protein covers a putative docking site on PHH for the PH reductase, which transfers electrons to the PHH diiron center prior to O2 activation, suggesting that the regulatory component may function to block undesired reduction of oxygenated intermediates during the catalytic cycle. A series of hydrophobic cavities through the PHH alpha-subunit, analogous to those in MMOH, may facilitate movement of the substrate to and/or product from the active site pocket. Comparisons between the ToMOH and PHH structures provide insights into their substrate regiospecificities.	+	The line below this paragraph, {{ABSTRACT_PUBMED_17176061}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17176061 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_17176061}}

	==About this Structure==		==About this Structure==
Line 31:		Line 35:
	[[Category: Hydroxylase]]		[[Category: Hydroxylase]]
	[[Category: Phenol]]		[[Category: Phenol]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 07~~:41:28 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:51:11 2008''

OCA at 04:41, 4 May 2008

2008-05-04T04:41:30Z

←Older revision		Revision as of 04:41, 4 May 2008
Line 1:		Line 1:
	[[Image:2inn.gif\|left\|200px]]		[[Image:2inn.gif\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 2inn \|SIZE=350\|CAPTION= <scene name='initialview01'>2inn</scene>~~, ~~resolution 2~~.~~700Å~~	+	The line below this paragraph, containing "STRUCTURE_2inn", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <scene name='pdbligand=FE:FE+~~(~~III~~)~~+ION'>FE</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY=~~	+	or leave the SCENE parameter empty for the default display.
-	~~\|GENE= phN~~ (~~[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]~~), ~~phL ([http://www~~.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), phO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), phM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri])	+	-->
-	~~\|DOMAIN=~~	+	{{STRUCTURE_2inn\| PDB=2inn \| SCENE= }}
-	\|~~RELATEDENTRY~~=	+
-	\|~~RESOURCES~~=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2inn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2inn OCA], [http://www.ebi.ac.uk/pdbsum/2inn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2inn RCSB]</span>	+
-	}}	+

	'''Structure of the Phenol Hydroxyalse-Regulatory Protein Complex'''		'''Structure of the Phenol Hydroxyalse-Regulatory Protein Complex'''
Line 29:		Line 26:
	[[Category: McCormick, M S.]]		[[Category: McCormick, M S.]]
	[[Category: Sazinsky, M H.]]		[[Category: Sazinsky, M H.]]
-	[[Category: ~~complex~~]]	+	[[Category: Complex]]
-	[[Category: ~~diiron~~]]	+	[[Category: Diiron]]
-	[[Category: ~~four~~-helix bundle]]	+	[[Category: Four-helix bundle]]
-	[[Category: ~~hydroxylase~~]]	+	[[Category: Hydroxylase]]
-	[[Category: ~~phenol~~]]	+	[[Category: Phenol]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:41:28 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 03~~:46:28 2008''	+