2iur - Revision history

OCA at 07:55, 4 November 2020

2020-11-04T07:55:06Z

←Older revision		Revision as of 07:55, 4 November 2020
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	==CRYSTAL STRUCTURE OF N-QUINOL FORM OF AROMATIC AMINE DEHYDROGENASE (AADH) FROM ALCALIGENES FAECALIS, FORM A COCRYSTAL==		==CRYSTAL STRUCTURE OF N-QUINOL FORM OF AROMATIC AMINE DEHYDROGENASE (AADH) FROM ALCALIGENES FAECALIS, FORM A COCRYSTAL==
-	<StructureSection load='2iur' size='340' side='right' caption='[[2iur]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='2iur' size='340' side='right'caption='[[2iur]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2iur]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUR OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2IUR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2iur]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2IUR FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TQQ:(S)-2-AMINO-3-(6,7-DIHYDRO-6-IMINO-7-OXO-1H-INDOL-3-YL)PROPANOIC+ACID'>TQQ</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TQQ:(S)-2-AMINO-3-(6,7-DIHYDRO-6-IMINO-7-OXO-1H-INDOL-3-YL)PROPANOIC+ACID'>TQQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iup\|2iup]], [[2iuq\|2iuq]], [[2iuv\|2iuv]], [[2iva\|2iva]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iup\|2iup]], [[2iuq\|2iuq]], [[2iuv\|2iuv]], [[2iva\|2iva]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aralkylamine_dehydrogenase_(azurin) Aralkylamine dehydrogenase (azurin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.9.2 1.4.9.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aralkylamine_dehydrogenase_(azurin) Aralkylamine dehydrogenase (azurin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.9.2 1.4.9.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2iur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iur OCA], [http://pdbe.org/2iur PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iur RCSB], [http://www.ebi.ac.uk/pdbsum/2iur PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2iur ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2iur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iur OCA], [http://pdbe.org/2iur PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iur RCSB], [http://www.ebi.ac.uk/pdbsum/2iur PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2iur ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
-	*[[Aromatic amine dehydrogenase\|Aromatic amine dehydrogenase]]	+	*[[Aromatic amine dehydrogenase 3D structures\|Aromatic amine dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Alcaligenes faecalis]]		[[Category: Alcaligenes faecalis]]
		+	[[Category: Large Structures]]
	[[Category: Leys, D]]		[[Category: Leys, D]]
	[[Category: Roujeinikova, A]]		[[Category: Roujeinikova, A]]
	[[Category: Scrutton, N]]		[[Category: Scrutton, N]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 08:36, 4 July 2018

2018-07-04T08:36:26Z

←Older revision		Revision as of 08:36, 4 July 2018
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF N-QUINOL FORM OF AROMATIC AMINE DEHYDROGENASE (AADH) FROM ALCALIGENES FAECALIS, FORM A COCRYSTAL==		==CRYSTAL STRUCTURE OF N-QUINOL FORM OF AROMATIC AMINE DEHYDROGENASE (AADH) FROM ALCALIGENES FAECALIS, FORM A COCRYSTAL==
	<StructureSection load='2iur' size='340' side='right' caption='[[2iur]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='2iur' size='340' side='right' caption='[[2iur]], [[Resolution\|resolution]] 1.30Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iup\|2iup]], [[2iuq\|2iuq]], [[2iuv\|2iuv]], [[2iva\|2iva]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iup\|2iup]], [[2iuq\|2iuq]], [[2iuv\|2iuv]], [[2iva\|2iva]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aralkylamine_dehydrogenase_(azurin) Aralkylamine dehydrogenase (azurin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.9.2 1.4.9.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aralkylamine_dehydrogenase_(azurin) Aralkylamine dehydrogenase (azurin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.9.2 1.4.9.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iur OCA], [http://pdbe.org/2iur PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iur RCSB], [http://www.ebi.ac.uk/pdbsum/2iur PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iur OCA], [http://pdbe.org/2iur PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iur RCSB], [http://www.ebi.ac.uk/pdbsum/2iur PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2iur ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/2iur_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/2iur_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 07:50, 7 February 2016

2016-02-07T07:50:23Z

←Older revision		Revision as of 07:50, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iur ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:21, 9 September 2015

2015-09-09T23:21:43Z

←Older revision

Revision as of 23:21, 9 September 2015

OCA at 08:40, 16 January 2015

2015-01-16T08:40:58Z

←Older revision		Revision as of 08:40, 16 January 2015
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2iur]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IUR FirstGlance]. <br>		<table><tr><td colspan='2'>[[2iur]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IUR FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TQQ:(S)-2-AMINO-3-(6,7-DIHYDRO-6-IMINO-7-OXO-1H-INDOL-3-YL)PROPANOIC+ACID'>TQQ</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TQQ:(S)-2-AMINO-3-(6,7-DIHYDRO-6-IMINO-7-OXO-1H-INDOL-3-YL)PROPANOIC+ACID'>TQQ</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iup\|2iup]], [[2iuq\|2iuq]], [[2iuv\|2iuv]], [[2iva\|2iva]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iup\|2iup]], [[2iuq\|2iuq]], [[2iuv\|2iuv]], [[2iva\|2iva]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aralkylamine_dehydrogenase Aralkylamine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.4 1.4.99.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aralkylamine_dehydrogenase Aralkylamine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.4 1.4.99.4] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iur OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iur RCSB], [http://www.ebi.ac.uk/pdbsum/2iur PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iur OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iur RCSB], [http://www.ebi.ac.uk/pdbsum/2iur PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Alcaligenes faecalis]]		[[Category: Alcaligenes faecalis]]
	[[Category: Aralkylamine dehydrogenase]]		[[Category: Aralkylamine dehydrogenase]]
-	[[Category: Leys, D.]]	+	[[Category: Leys, D]]
-	[[Category: Roujeinikova, A.]]	+	[[Category: Roujeinikova, A]]
-	[[Category: Scrutton, N.]]	+	[[Category: Scrutton, N]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 10:15, 30 September 2014

2014-09-30T10:15:09Z

←Older revision		Revision as of 10:15, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2iur.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF N-QUINOL FORM OF AROMATIC AMINE DEHYDROGENASE (AADH) FROM ALCALIGENES FAECALIS, FORM A COCRYSTAL==
		+	<StructureSection load='2iur' size='340' side='right' caption='[[2iur]], [[Resolution\|resolution]] 1.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2iur]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IUR FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TQQ:(S)-2-AMINO-3-(6,7-DIHYDRO-6-IMINO-7-OXO-1H-INDOL-3-YL)PROPANOIC+ACID'>TQQ</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iup\|2iup]], [[2iuq\|2iuq]], [[2iuv\|2iuv]], [[2iva\|2iva]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aralkylamine_dehydrogenase Aralkylamine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.99.4 1.4.99.4] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iur FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iur OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iur RCSB], [http://www.ebi.ac.uk/pdbsum/2iur PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/2iur_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The quinoprotein aromatic amine dehydrogenase (AADH) uses a covalently bound tryptophan tryptophylquinone (TTQ) cofactor to oxidatively deaminate primary aromatic amines. Recent crystal structures have provided insight into the reductive half-reaction. In contrast, no atomic details are available for the oxidative half-reaction. The TTQ O7 hydroxyl group is protonated during reduction, but it is unclear how this proton can be removed during the oxidative half-reaction. Furthermore, compared with the electron transfer from the N-quinol form, electron transfer from the non-physiological O-quinol form to azurin is significantly slower. Here we report crystal structures of the O-quinol, N-quinol, and N-semiquinone forms of AADH. A comparison of oxidized and substrate reduced AADH species reveals changes in the TTQ-containing subunit, extending from residues in the immediate vicinity of the N-quinol to the putative azurin docking site, suggesting a mechanism whereby TTQ redox state influences interprotein electron transfer. In contrast, chemical reduction of the TTQ center has no significant effect on protein conformation. Furthermore, structural reorganization upon substrate reduction places a water molecule near TTQ O7 where it can act as proton acceptor. The structure of the N-semiquinone, however, is essentially similar to oxidized AADH. Surprisingly, in the presence of substrate a covalent N-semiquinone substrate adduct is observed. To our knowledge this is the first detailed insight into a complex, branching mechanism of quinone oxidation where significant structural reorganization upon reduction of the quinone center directly influences formation of the electron transfer complex and nature of the electron transfer process.

-	~~{{STRUCTURE_2iur\| PDB=2iur \| SCENE= }}~~	+	Atomic level insight into the oxidative half-reaction of aromatic amine dehydrogenase.,Roujeinikova A, Scrutton NS, Leys D J Biol Chem. 2006 Dec 29;281(52):40264-72. Epub 2006 Sep 27. PMID:17005560<ref>PMID:17005560</ref>

-	~~===CRYSTAL STRUCTURE OF N-QUINOL FORM OF AROMATIC AMINE DEHYDROGENASE (AADH) FROM ALCALIGENES FAECALIS~~, ~~FORM A COCRYSTAL===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_17005560}}~~	+	==See Also==
-		+	*[[Aromatic amine dehydrogenase\|Aromatic amine dehydrogenase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~2iur~~]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUR OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:017005560<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Alcaligenes faecalis]]		[[Category: Alcaligenes faecalis]]
	[[Category: Aralkylamine dehydrogenase]]		[[Category: Aralkylamine dehydrogenase]]

OCA: Protected "2iur" [edit=sysop:move=sysop]

2013-01-07T12:04:57Z

Protected "2iur" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:04, 7 January 2013

OCA at 12:04, 7 January 2013

2013-01-07T12:04:54Z

←Older revision		Revision as of 12:04, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2iur.png\|left\|200px]]		[[Image:2iur.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2iur", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2iur\| PDB=2iur \| SCENE= }}		{{STRUCTURE_2iur\| PDB=2iur \| SCENE= }}

	===CRYSTAL STRUCTURE OF N-QUINOL FORM OF AROMATIC AMINE DEHYDROGENASE (AADH) FROM ALCALIGENES FAECALIS, FORM A COCRYSTAL===		===CRYSTAL STRUCTURE OF N-QUINOL FORM OF AROMATIC AMINE DEHYDROGENASE (AADH) FROM ALCALIGENES FAECALIS, FORM A COCRYSTAL===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17005560}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17005560 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17005560}}		{{ABSTRACT_PUBMED_17005560}}

	==About this Structure==		==About this Structure==
-	~~2IUR~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUR OCA].	+	[[2iur]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUR OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17005560~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017005560</ref><references group="xtra"/>
	[[Category: Alcaligenes faecalis]]		[[Category: Alcaligenes faecalis]]
	[[Category: Aralkylamine dehydrogenase]]		[[Category: Aralkylamine dehydrogenase]]
Line 30:		Line 18:
	[[Category: Scrutton, N.]]		[[Category: Scrutton, N.]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:22:55 2009''

OCA at 10:22, 16 February 2009

2009-02-16T10:22:56Z

←Older revision		Revision as of 10:22, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2IUR is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUR OCA].	+	2IUR is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUR OCA].

	==Reference==		==Reference==
-	~~Atomic level insight into the oxidative half-reaction of aromatic amine dehydrogenase., Roujeinikova A, Scrutton NS, Leys D, J Biol Chem. 2006 Dec 29;281(52):40264-72. Epub 2006 Sep 27.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17005560 17005560]~~	+	<ref group="xtra">PMID:17005560</ref><references group="xtra"/>
	[[Category: Alcaligenes faecalis]]		[[Category: Alcaligenes faecalis]]
	[[Category: Aralkylamine dehydrogenase]]		[[Category: Aralkylamine dehydrogenase]]
-	[[Category: Single protein]]
	[[Category: Leys, D.]]		[[Category: Leys, D.]]
	[[Category: Roujeinikova, A.]]		[[Category: Roujeinikova, A.]]
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	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

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OCA at 11:34, 27 July 2008

2008-07-27T11:34:56Z

←Older revision		Revision as of 11:34, 27 July 2008
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-	[[Image:2iur.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2iur.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2iur\| PDB=2iur \| SCENE= }}		{{STRUCTURE_2iur\| PDB=2iur \| SCENE= }}

-	~~'''~~CRYSTAL STRUCTURE OF N-QUINOL FORM OF AROMATIC AMINE DEHYDROGENASE (AADH) FROM ALCALIGENES FAECALIS, FORM A COCRYSTAL~~'''~~	+	===CRYSTAL STRUCTURE OF N-QUINOL FORM OF AROMATIC AMINE DEHYDROGENASE (AADH) FROM ALCALIGENES FAECALIS, FORM A COCRYSTAL===


-	~~==Overview==~~	+	<!--
-	The quinoprotein aromatic amine dehydrogenase (AADH) uses a covalently bound tryptophan tryptophylquinone (TTQ) cofactor to oxidatively deaminate primary aromatic amines. Recent crystal structures have provided insight into the reductive half-~~reaction. In contrast, no atomic details are available for the oxidative half~~-~~reaction.~~ The ~~TTQ O7 hydroxyl group is protonated during reduction, but it is unclear how~~ this ~~proton can be removed during the oxidative half-reaction. Furthermore~~, ~~compared with the electron transfer from the N-quinol form~~, ~~electron transfer from~~ the ~~non-physiological O-quinol form~~ to ~~azurin is significantly slower. Here we report crystal structures of~~ the ~~O-quinol, N-quinol, and N-semiquinone forms of AADH~~. A comparison of oxidized and substrate reduced AADH species reveals changes in the TTQ-containing subunit, extending from residues in the immediate vicinity of the N-quinol to the putative azurin docking site, suggesting a mechanism whereby TTQ redox state influences interprotein electron transfer. ~~In contrast~~, ~~chemical reduction of the TTQ center has no significant effect on protein conformation. Furthermore, structural reorganization upon substrate reduction places a water molecule near TTQ O7~~ where ~~it can act as proton acceptor. The structure of~~ the ~~N-semiquinone, however, is essentially similar to oxidized AADH~~. ~~Surprisingly, in the presence of substrate a covalent N~~-semiquinone substrate adduct is observed. To our knowledge this is the first detailed insight into a complex, branching mechanism of quinone oxidation where significant structural reorganization upon reduction of the quinone center directly influences formation of the electron transfer complex and nature of the electron transfer process.	+	The line below this paragraph, {{ABSTRACT_PUBMED_17005560}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17005560 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_17005560}}

	==About this Structure==		==About this Structure==
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	[[Category: Scrutton, N.]]		[[Category: Scrutton, N.]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
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