2j62 - Revision history

OCA at 09:34, 9 May 2024

2024-05-09T09:34:43Z

←Older revision		Revision as of 09:34, 9 May 2024
Line 3:		Line 3:
	<StructureSection load='2j62' size='340' side='right'caption='[[2j62]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='2j62' size='340' side='right'caption='[[2j62]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j62]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus~~ perfringens~~" veillon and zuber 1898~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J62 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j62]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J62 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GSZ:N-[(5R,6R,7R,8S)-6,7-DIHYDROXY-5-(HYDROXYMETHYL)-2-(2-PHENYLETHYL)-1,5,6,7,8,8A-HEXAHYDROIMIDAZO[1,2-A]PYRIDIN-8-YL]-2-METHYLPROPANAMIDE'>GSZ</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.26Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nagJ, CPF_1442 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1502 "Bacillus perfringens" Veillon and Zuber 1898])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GSZ:N-[(5R,6R,7R,8S)-6,7-DIHYDROXY-5-(HYDROXYMETHYL)-2-(2-PHENYLETHYL)-1,5,6,7,8,8A-HEXAHYDROIMIDAZO[1,2-A]PYRIDIN-8-YL]-2-METHYLPROPANAMIDE'>GSZ</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Protein_O-GlcNAcase Protein O-GlcNAcase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.169 3.2.1.169] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j62 OCA], [https://pdbe.org/2j62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j62 RCSB], [https://www.ebi.ac.uk/pdbsum/2j62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j62 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j62 OCA], [https://pdbe.org/2j62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j62 RCSB], [https://www.ebi.ac.uk/pdbsum/2j62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j62 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/OGA_CLOP1 OGA_CLOP1]] Biological function unknown. Capable of hydrolyzing the glycosidic link of O-GlcNAcylated proteins.	+	[https://www.uniprot.org/uniprot/OGA_CLOP1 OGA_CLOP1] Biological function unknown. Capable of hydrolyzing the glycosidic link of O-GlcNAcylated proteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ perfringens ~~veillon and zuber 1898~~]]	+	[[Category: Clostridium perfringens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Protein O-GlcNAcase]]~~	+	[[Category: Borodkin VS]]
-	~~[[Category: Aalten, D M.F van]]~~	+	[[Category: Dorfmueller HC]]
-	[[Category: Borodkin~~, V S~~]]	+	[[Category: Schimpl M]]
-	[[Category: Dorfmueller~~, H C~~]]	+	[[Category: Shepherd SM]]
-	[[Category: Schimpl, M]]	+	[[Category: Shpiro NA]]
-	[[Category: Shepherd~~, S M~~]]	+	[[Category: Van Aalten DMF]]
-	[[Category: Shpiro~~, N A~~]]	+
-	[[Category: ~~Hydrolase~~]]	+

OCA at 11:24, 31 March 2021

2021-03-31T11:24:58Z

←Older revision		Revision as of 11:24, 31 March 2021
Line 1:		Line 1:

	==Structure of a bacterial O-glcnacase in complex with glcnacstatin==		==Structure of a bacterial O-glcnacase in complex with glcnacstatin==
-	<StructureSection load='2j62' size='340' side='right' caption='[[2j62]], [[Resolution\|resolution]] 2.26Å' scene=''>	+	<StructureSection load='2j62' size='340' side='right'caption='[[2j62]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j62]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2J62 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j62]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J62 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GSZ:N-[(5R,6R,7R,8S)-6,7-DIHYDROXY-5-(HYDROXYMETHYL)-2-(2-PHENYLETHYL)-1,5,6,7,8,8A-HEXAHYDROIMIDAZO[1,2-A]PYRIDIN-8-YL]-2-METHYLPROPANAMIDE'>GSZ</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GSZ:N-[(5R,6R,7R,8S)-6,7-DIHYDROXY-5-(HYDROXYMETHYL)-2-(2-PHENYLETHYL)-1,5,6,7,8,8A-HEXAHYDROIMIDAZO[1,2-A]PYRIDIN-8-YL]-2-METHYLPROPANAMIDE'>GSZ</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nagJ, CPF_1442 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1502 "Bacillus perfringens" Veillon and Zuber 1898])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nagJ, CPF_1442 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1502 "Bacillus perfringens" Veillon and Zuber 1898])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Protein_O-GlcNAcase Protein O-GlcNAcase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.169 3.2.1.169] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Protein_O-GlcNAcase Protein O-GlcNAcase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.169 3.2.1.169] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j62 OCA], [~~http~~://pdbe.org/2j62 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2j62 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2j62 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2j62 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j62 OCA], [https://pdbe.org/2j62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j62 RCSB], [https://www.ebi.ac.uk/pdbsum/2j62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j62 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/OGA_CLOP1 OGA_CLOP1]] Biological function unknown. Capable of hydrolyzing the glycosidic link of O-GlcNAcylated proteins.	+	[[https://www.uniprot.org/uniprot/OGA_CLOP1 OGA_CLOP1]] Biological function unknown. Capable of hydrolyzing the glycosidic link of O-GlcNAcylated proteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Hyaluronidase\|Hyaluronidase]]	+	*[[Hyaluronidase 3D structures\|Hyaluronidase 3D structures]]
		+	*[[O-GlcNAcase\|O-GlcNAcase]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus perfringens veillon and zuber 1898]]		[[Category: Bacillus perfringens veillon and zuber 1898]]
		+	[[Category: Large Structures]]
	[[Category: Protein O-GlcNAcase]]		[[Category: Protein O-GlcNAcase]]
	[[Category: Aalten, D M.F van]]		[[Category: Aalten, D M.F van]]

OCA at 20:39, 2 December 2018

2018-12-02T20:39:57Z

←Older revision		Revision as of 20:39, 2 December 2018
Line 3:		Line 3:
	<StructureSection load='2j62' size='340' side='right' caption='[[2j62]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='2j62' size='340' side='right' caption='[[2j62]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j62]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J62 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j62]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_perfringens"_veillon_and_zuber_1898 "bacillus perfringens" veillon and zuber 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J62 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GSZ:N-[(5R,6R,7R,8S)-6,7-DIHYDROXY-5-(HYDROXYMETHYL)-2-(2-PHENYLETHYL)-1,5,6,7,8,8A-HEXAHYDROIMIDAZO[1,2-A]PYRIDIN-8-YL]-2-METHYLPROPANAMIDE'>GSZ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GSZ:N-[(5R,6R,7R,8S)-6,7-DIHYDROXY-5-(HYDROXYMETHYL)-2-(2-PHENYLETHYL)-1,5,6,7,8,8A-HEXAHYDROIMIDAZO[1,2-A]PYRIDIN-8-YL]-2-METHYLPROPANAMIDE'>GSZ</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nagJ, CPF_1442 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1502 "Bacillus perfringens" Veillon and Zuber 1898])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_O-GlcNAcase Protein O-GlcNAcase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.169 3.2.1.169] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j62 OCA], [http://pdbe.org/2j62 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j62 RCSB], [http://www.ebi.ac.uk/pdbsum/2j62 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j62 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j62 OCA], [http://pdbe.org/2j62 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j62 RCSB], [http://www.ebi.ac.uk/pdbsum/2j62 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j62 ProSAT]</span></td></tr>
	</table>		</table>
Line 35:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus perfringens veillon and zuber 1898]]
		+	[[Category: Protein O-GlcNAcase]]
	[[Category: Aalten, D M.F van]]		[[Category: Aalten, D M.F van]]
	[[Category: Borodkin, V S]]		[[Category: Borodkin, V S]]

OCA at 06:45, 11 July 2018

2018-07-11T06:45:01Z

←Older revision		Revision as of 06:45, 11 July 2018
Line 1:		Line 1:
-	==~~STRUCTURE OF A BACTERIAL~~ O-~~GLCNACASE IN COMPLEX WITH GLCNACSTATIN~~==	+
		+	==Structure of a bacterial O-glcnacase in complex with glcnacstatin==
	<StructureSection load='2j62' size='340' side='right' caption='[[2j62]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='2j62' size='340' side='right' caption='[[2j62]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2j62]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J62 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2j62]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J62 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GSZ:N-[(5R,6R,7R,8S)-6,7-DIHYDROXY-5-(HYDROXYMETHYL)-2-(2-PHENYLETHYL)-1,5,6,7,8,8A-HEXAHYDROIMIDAZO[1,2-A]PYRIDIN-8-YL]-2-METHYLPROPANAMIDE'>GSZ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GSZ:N-[(5R,6R,7R,8S)-6,7-DIHYDROXY-5-(HYDROXYMETHYL)-2-(2-PHENYLETHYL)-1,5,6,7,8,8A-HEXAHYDROIMIDAZO[1,2-A]PYRIDIN-8-YL]-2-METHYLPROPANAMIDE'>GSZ</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j62 OCA], [http://pdbe.org/2j62 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j62 RCSB], [http://www.ebi.ac.uk/pdbsum/2j62 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j62 OCA], [http://pdbe.org/2j62 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j62 RCSB], [http://www.ebi.ac.uk/pdbsum/2j62 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j62 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j6/2j62_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j6/2j62_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j62 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aalten, D M.F ~~Van~~]]	+	[[Category: Aalten, D M.F van]]
	[[Category: Borodkin, V S]]		[[Category: Borodkin, V S]]
	[[Category: Dorfmueller, H C]]		[[Category: Dorfmueller, H C]]

OCA at 15:12, 11 September 2015

2015-09-11T15:12:52Z

←Older revision		Revision as of 15:12, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[2j62]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J62 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2j62]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J62 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GSZ:N-[(5R,6R,7R,8S)-6,7-DIHYDROXY-5-(HYDROXYMETHYL)-2-(2-PHENYLETHYL)-1,5,6,7,8,8A-HEXAHYDROIMIDAZO[1,2-A]PYRIDIN-8-YL]-2-METHYLPROPANAMIDE'>GSZ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GSZ:N-[(5R,6R,7R,8S)-6,7-DIHYDROXY-5-(HYDROXYMETHYL)-2-(2-PHENYLETHYL)-1,5,6,7,8,8A-HEXAHYDROIMIDAZO[1,2-A]PYRIDIN-8-YL]-2-METHYLPROPANAMIDE'>GSZ</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j62 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j62 RCSB], [http://www.ebi.ac.uk/pdbsum/2j62 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j62 OCA], [http://pdbe.org/2j62 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j62 RCSB], [http://www.ebi.ac.uk/pdbsum/2j62 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/OGA_CLOP1 OGA_CLOP1]] Biological function unknown. Capable of hydrolyzing the glycosidic link of O-GlcNAcylated proteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2j62" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 16:36, 19 January 2015

2015-01-19T16:36:00Z

←Older revision		Revision as of 16:36, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2j62]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J62 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2j62]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J62 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GSZ:N-[(5R,6R,7R,8S)-6,7-DIHYDROXY-5-(HYDROXYMETHYL)-2-(2-PHENYLETHYL)-1,5,6,7,8,8A-HEXAHYDROIMIDAZO[1,2-A]PYRIDIN-8-YL]-2-METHYLPROPANAMIDE'>GSZ</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GSZ:N-[(5R,6R,7R,8S)-6,7-DIHYDROXY-5-(HYDROXYMETHYL)-2-(2-PHENYLETHYL)-1,5,6,7,8,8A-HEXAHYDROIMIDAZO[1,2-A]PYRIDIN-8-YL]-2-METHYLPROPANAMIDE'>GSZ</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j62 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j62 RCSB], [http://www.ebi.ac.uk/pdbsum/2j62 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j62 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j62 RCSB], [http://www.ebi.ac.uk/pdbsum/2j62 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aalten, D M.F Van.]]	+	[[Category: Aalten, D M.F Van]]
-	[[Category: Borodkin, V S.]]	+	[[Category: Borodkin, V S]]
-	[[Category: Dorfmueller, H C.]]	+	[[Category: Dorfmueller, H C]]
-	[[Category: Schimpl, M.]]	+	[[Category: Schimpl, M]]
-	[[Category: Shepherd, S M.]]	+	[[Category: Shepherd, S M]]
-	[[Category: Shpiro, N A.]]	+	[[Category: Shpiro, N A]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 05:39, 29 September 2014

2014-09-29T05:39:17Z

←Older revision		Revision as of 05:39, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2j62.~~png\|left~~\|200px]]	+	==STRUCTURE OF A BACTERIAL O-GLCNACASE IN COMPLEX WITH GLCNACSTATIN==
		+	<StructureSection load='2j62' size='340' side='right' caption='[[2j62]], [[Resolution\|resolution]] 2.26Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2j62]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J62 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GSZ:N-[(5R,6R,7R,8S)-6,7-DIHYDROXY-5-(HYDROXYMETHYL)-2-(2-PHENYLETHYL)-1,5,6,7,8,8A-HEXAHYDROIMIDAZO[1,2-A]PYRIDIN-8-YL]-2-METHYLPROPANAMIDE'>GSZ</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j62 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j62 RCSB], [http://www.ebi.ac.uk/pdbsum/2j62 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j6/2j62_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many phosphorylation signal transduction pathways in the eukaryotic cell are modulated by posttranslational modification of specific serines/threonines with N-acetylglucosamine (O-GlcNAc). Levels of O-GlcNAc on key proteins regulate biological processes as diverse as the cell cycle, insulin signaling, and protein degradation. The two enzymes involved in this dynamic and abundant modification are the O-GlcNAc transferase and O-GlcNAcase. Structural data have recently revealed that the O-GlcNAcase possesses an active site with significant structural similarity to that of the human lysosomal hexosaminidases HexA/HexB. PUGNAc, an O-GlcNAcase inhibitor widely used to raise levels of O-GlcNAc in human cell lines, also inhibits these hexosaminidases. Here, we have exploited recent structural information of an O-GlcNAcase-PUGNAc complex to design and synthesize a glucoimidazole-based inhibitor, GlcNAcstatin, which is a 5 pM competitive inhibitor of enzymes of the O-GlcNAcase family, shows 100000-fold selectivity over HexA/B, and binds to the O-GlcNAcase active site by mimicking the transition state as revealed by X-ray crystallography. This compound is able to raise O-GlcNAc levels in human HEK 293 and SH-SY5Y neuroblastoma cell lines and thus provides a novel, potent tool for the study of the role of O-GlcNAc in intracellular signal transduction pathways.

-	~~{{STRUCTURE_2j62\| PDB=2j62 \| SCENE= }}~~	+	GlcNAcstatin: a picomolar, selective O-GlcNAcase inhibitor that modulates intracellular O-glcNAcylation levels.,Dorfmueller HC, Borodkin VS, Schimpl M, Shepherd SM, Shpiro NA, van Aalten DM J Am Chem Soc. 2006 Dec 27;128(51):16484-5. PMID:17177381<ref>PMID:17177381</ref>

-	~~===STRUCTURE OF A BACTERIAL O-GLCNACASE IN COMPLEX WITH GLCNACSTATIN===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17177381}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2j62]] is~~ a ~~2 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2J62 OCA].~~	+

	==See Also==		==See Also==
	*[[Hyaluronidase\|Hyaluronidase]]		*[[Hyaluronidase\|Hyaluronidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:017177381<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Aalten, D M.F Van.]]		[[Category: Aalten, D M.F Van.]]
	[[Category: Borodkin, V S.]]		[[Category: Borodkin, V S.]]

OCA at 19:50, 20 October 2012

2012-10-20T19:50:53Z

←Older revision		Revision as of 19:50, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2j62]] is a 2 chain structure ~~of [[Hyaluronidase]]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA].	+	[[2j62]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA].

	==See Also==		==See Also==

OCA at 08:40, 27 July 2012

2012-07-27T08:40:18Z

←Older revision		Revision as of 08:40, 27 July 2012
Line 1:		Line 1:
	[[Image:2j62.png\|left\|200px]]		[[Image:2j62.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2j62", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2j62\| PDB=2j62 \| SCENE= }}		{{STRUCTURE_2j62\| PDB=2j62 \| SCENE= }}

	===STRUCTURE OF A BACTERIAL O-GLCNACASE IN COMPLEX WITH GLCNACSTATIN===		===STRUCTURE OF A BACTERIAL O-GLCNACASE IN COMPLEX WITH GLCNACSTATIN===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17177381}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17177381 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17177381}}		{{ABSTRACT_PUBMED_17177381}}

	==About this Structure==		==About this Structure==
-	[[2j62]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA].	+	[[2j62]] is a 2 chain structure of [[Hyaluronidase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J62 OCA].
		+
		+	==See Also==
		+	*[[Hyaluronidase\|Hyaluronidase]]

	==Reference==		==Reference==

OCA: Protected "2j62" [edit=sysop:move=sysop]

2011-08-24T05:34:15Z

Protected "2j62" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:34, 24 August 2011