2jcs - Revision history

OCA at 14:43, 13 December 2023

2023-12-13T14:43:27Z

←Older revision		Revision as of 14:43, 13 December 2023
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	<StructureSection load='2jcs' size='340' side='right'caption='[[2jcs]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2jcs' size='340' side='right'caption='[[2jcs]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jcs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Drome Drome~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JCS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jcs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JCS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~, ~~<scene name='pdbligand=TTP:THYMIDINE-~~5~~-TRIPHOSPHATE'>TTP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1j90\|1j90]], [[1oe0\|1oe0]], [[1ot3\|1ot3]], [[1zm7\|1zm7]], [[1zmx\|1zmx]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jcs OCA], [https://pdbe.org/2jcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jcs RCSB], [https://www.ebi.ac.uk/pdbsum/2jcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jcs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jcs OCA], [https://pdbe.org/2jcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jcs RCSB], [https://www.ebi.ac.uk/pdbsum/2jcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jcs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DNK_DROME DNK_DROME]] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.<ref>PMID:10446143</ref> <ref>PMID:10692477</ref> <ref>PMID:16008571</ref>	+	[https://www.uniprot.org/uniprot/DNK_DROME DNK_DROME] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.<ref>PMID:10446143</ref> <ref>PMID:10692477</ref> <ref>PMID:16008571</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</div>		</div>
	<div class="pdbe-citations 2jcs" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2jcs" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Thymidine kinase\|Thymidine kinase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Deoxynucleoside kinase]]~~	+	[[Category: Drosophila melanogaster]]
-	~~[[Category: Drome~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Egeblad-Welin, L]]	+	[[Category: Egeblad-Welin L]]
-	[[Category: Eklund, H]]	+	[[Category: Eklund H]]
-	[[Category: Munch-Petersen, B]]	+	[[Category: Munch-Petersen B]]
-	[[Category: Sonntag, Y]]	+	[[Category: Sonntag Y]]
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Deoxyribonucleoside kinase]]~~	+
-	~~[[Category: Dna synthesis]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Nucleotide- binding]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 20:44, 20 October 2021

2021-10-20T20:44:46Z

←Older revision		Revision as of 20:44, 20 October 2021
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	<StructureSection load='2jcs' size='340' side='right'caption='[[2jcs]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2jcs' size='340' side='right'caption='[[2jcs]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jcs]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2JCS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jcs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JCS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j90\|1j90]], [[1oe0\|1oe0]], [[1ot3\|1ot3]], [[1zm7\|1zm7]], [[1zmx\|1zmx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1j90\|1j90]], [[1oe0\|1oe0]], [[1ot3\|1ot3]], [[1zm7\|1zm7]], [[1zmx\|1zmx]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2jcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jcs OCA], [~~http~~://pdbe.org/2jcs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2jcs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2jcs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2jcs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jcs OCA], [https://pdbe.org/2jcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jcs RCSB], [https://www.ebi.ac.uk/pdbsum/2jcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jcs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DNK_DROME DNK_DROME]] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.<ref>PMID:10446143</ref> <ref>PMID:10692477</ref> <ref>PMID:16008571</ref>	+	[[https://www.uniprot.org/uniprot/DNK_DROME DNK_DROME]] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.<ref>PMID:10446143</ref> <ref>PMID:10692477</ref> <ref>PMID:16008571</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</div>		</div>
	<div class="pdbe-citations 2jcs" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2jcs" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Thymidine kinase\|Thymidine kinase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:41, 11 December 2019

2019-12-11T19:41:58Z

←Older revision		Revision as of 19:41, 11 December 2019
Line 1:		Line 1:

	==Active site mutant of dNK from D. melanogaster with dTTP bound==		==Active site mutant of dNK from D. melanogaster with dTTP bound==
-	<StructureSection load='2jcs' size='340' side='right' caption='[[2jcs]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='2jcs' size='340' side='right'caption='[[2jcs]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2jcs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JCS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2jcs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JCS FirstGlance]. <br>
Line 36:		Line 36:
	[[Category: Deoxynucleoside kinase]]		[[Category: Deoxynucleoside kinase]]
	[[Category: Drome]]		[[Category: Drome]]
		+	[[Category: Large Structures]]
	[[Category: Egeblad-Welin, L]]		[[Category: Egeblad-Welin, L]]
	[[Category: Eklund, H]]		[[Category: Eklund, H]]

OCA at 07:23, 17 January 2018

2018-01-17T07:23:00Z

←Older revision		Revision as of 07:23, 17 January 2018
Line 1:		Line 1:
-	==~~ACTIVE SITE MUTANT OF DNK FROM~~ D. ~~MELANOGASTER WITH DTTP BOUND~~==	+
		+	==Active site mutant of dNK from D. melanogaster with dTTP bound==
	<StructureSection load='2jcs' size='340' side='right' caption='[[2jcs]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2jcs' size='340' side='right' caption='[[2jcs]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j90\|1j90]], [[1oe0\|1oe0]], [[1ot3\|1ot3]], [[1zm7\|1zm7]], [[1zmx\|1zmx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j90\|1j90]], [[1oe0\|1oe0]], [[1ot3\|1ot3]], [[1zm7\|1zm7]], [[1zmx\|1zmx]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jcs OCA], [http://pdbe.org/2jcs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jcs RCSB], [http://www.ebi.ac.uk/pdbsum/2jcs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jcs OCA], [http://pdbe.org/2jcs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jcs RCSB], [http://www.ebi.ac.uk/pdbsum/2jcs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jcs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jc/2jcs_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jc/2jcs_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 11:46, 7 February 2016

2016-02-07T11:46:50Z

←Older revision		Revision as of 11:46, 7 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jcs ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:11, 10 September 2015

2015-09-10T01:11:40Z

←Older revision		Revision as of 01:11, 10 September 2015
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	<StructureSection load='2jcs' size='340' side='right' caption='[[2jcs]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2jcs' size='340' side='right' caption='[[2jcs]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jcs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Drosophila_melanogaster Drosophila melanogaster~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JCS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jcs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JCS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j90\|1j90]], [[1oe0\|1oe0]], [[1ot3\|1ot3]], [[1zm7\|1zm7]], [[1zmx\|1zmx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j90\|1j90]], [[1oe0\|1oe0]], [[1ot3\|1ot3]], [[1zm7\|1zm7]], [[1zmx\|1zmx]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jcs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jcs RCSB], [http://www.ebi.ac.uk/pdbsum/2jcs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jcs OCA], [http://pdbe.org/2jcs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jcs RCSB], [http://www.ebi.ac.uk/pdbsum/2jcs PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2jcs" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Deoxynucleoside kinase]]		[[Category: Deoxynucleoside kinase]]
-	[[Category: ~~Drosophila melanogaster~~]]	+	[[Category: Drome]]
	[[Category: Egeblad-Welin, L]]		[[Category: Egeblad-Welin, L]]
	[[Category: Eklund, H]]		[[Category: Eklund, H]]

OCA at 14:50, 24 December 2014

2014-12-24T14:50:54Z

←Older revision		Revision as of 14:50, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2jcs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JCS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2jcs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JCS FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j90\|1j90]], [[1oe0\|1oe0]], [[1ot3\|1ot3]], [[1zm7\|1zm7]], [[1zmx\|1zmx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j90\|1j90]], [[1oe0\|1oe0]], [[1ot3\|1ot3]], [[1zm7\|1zm7]], [[1zmx\|1zmx]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jcs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jcs RCSB], [http://www.ebi.ac.uk/pdbsum/2jcs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jcs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jcs RCSB], [http://www.ebi.ac.uk/pdbsum/2jcs PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DNK_DROME DNK_DROME]] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.<ref>PMID:10446143</ref> <ref>PMID:10692477</ref> <ref>PMID:16008571</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	[[Category: Deoxynucleoside kinase]]		[[Category: Deoxynucleoside kinase]]
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
-	[[Category: Egeblad-Welin, L.]]	+	[[Category: Egeblad-Welin, L]]
-	[[Category: Eklund, H.]]	+	[[Category: Eklund, H]]
-	[[Category: Munch-Petersen, B.]]	+	[[Category: Munch-Petersen, B]]
-	[[Category: Sonntag, Y.]]	+	[[Category: Sonntag, Y]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Deoxyribonucleoside kinase]]		[[Category: Deoxyribonucleoside kinase]]

OCA at 08:17, 30 September 2014

2014-09-30T08:17:04Z

←Older revision		Revision as of 08:17, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2jcs.~~png~~\|~~left~~\|200px]]	+	==ACTIVE SITE MUTANT OF DNK FROM D. MELANOGASTER WITH DTTP BOUND==
		+	<StructureSection load='2jcs' size='340' side='right' caption='[[2jcs]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2jcs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JCS FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TTP:THYMIDINE-5-TRIPHOSPHATE'>TTP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j90\|1j90]], [[1oe0\|1oe0]], [[1ot3\|1ot3]], [[1zm7\|1zm7]], [[1zmx\|1zmx]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxynucleoside_kinase Deoxynucleoside kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.145 2.7.1.145] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jcs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jcs RCSB], [http://www.ebi.ac.uk/pdbsum/2jcs PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jc/2jcs_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The catalytic reaction mechanism and binding of substrates was investigated for the multisubstrate Drosophila melanogaster deoxyribonucleoside kinase. Mutation of E52 to D, Q and H plus mutations of R105 to K and H were performed to investigate the proposed catalytic reaction mechanism, in which E52 acts as an initiating base and R105 is thought to stabilize the transition state of the reaction. Mutant enzymes (E52D, E52H and R105H) showed a markedly decreased k(cat), while the catalytic activity of E52Q and R105K was abolished. The E52D mutant was crystallized with its feedback inhibitor dTTP. The backbone conformation remained unchanged, and coordination between D52 and the dTTP-Mg complex was observed. The observed decrease in k(cat) for E52D was most likely due to an increased distance between the catalytic carboxyl group and 5'-OH of deoxythymidine (dThd) or deoxycytidine (dCyd). Mutation of Q81 to N and Y70 to W was carried out to investigate substrate binding. The mutations primarily affected the K(m) values, whereas the k(cat) values were of the same magnitude as for the wild-type. The Y70W mutation made the enzyme lose activity towards purines and negative cooperativity towards dThd and dCyd was observed. The Q81N mutation showed a 200- and 100-fold increase in K(m), whereas k(cat) was decreased five- and twofold for dThd and dCyd, respectively, supporting a role in substrate binding. These observations give insight into the mechanisms of substrate binding and catalysis, which is important for developing novel suicide genes and drugs for use in gene therapy.

-	~~{{STRUCTURE_2jcs\| PDB=2jcs \| SCENE= }}~~	+	Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase. Investigations of the putative catalytic glutamate-arginine pair and of residues responsible for substrate specificity.,Egeblad-Welin L, Sonntag Y, Eklund H, Munch-Petersen B FEBS J. 2007 Mar;274(6):1542-51. PMID:17302737<ref>PMID:17302737</ref>

-	~~===ACTIVE SITE MUTANT OF DNK FROM D. MELANOGASTER WITH DTTP BOUND===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17302737}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2jcs]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Drosophila_melanogaster Drosophila melanogaster]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017302737<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Deoxynucleoside kinase]]		[[Category: Deoxynucleoside kinase]]
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]

OCA: Protected "2jcs" [edit=sysop:move=sysop]

2013-01-07T13:20:01Z

Protected "2jcs" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:20, 7 January 2013

OCA at 13:20, 7 January 2013

2013-01-07T13:20:00Z

←Older revision		Revision as of 13:20, 7 January 2013
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-	{{Seed}}
	[[Image:2jcs.png\|left\|200px]]		[[Image:2jcs.png\|left\|200px]]

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	{{STRUCTURE_2jcs\| PDB=2jcs \| SCENE= }}		{{STRUCTURE_2jcs\| PDB=2jcs \| SCENE= }}

	===ACTIVE SITE MUTANT OF DNK FROM D. MELANOGASTER WITH DTTP BOUND===		===ACTIVE SITE MUTANT OF DNK FROM D. MELANOGASTER WITH DTTP BOUND===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17302737}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_17302737}}		{{ABSTRACT_PUBMED_17302737}}

	==About this Structure==		==About this Structure==
-	~~2JCS~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA].	+	[[2jcs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCS OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17302737~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017302737</ref><references group="xtra"/>
	[[Category: Deoxynucleoside kinase]]		[[Category: Deoxynucleoside kinase]]
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
Line 36:		Line 24:
	[[Category: Nucleotide- binding]]		[[Category: Nucleotide- binding]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 22 11:28:17 2009''