2je9 - Revision history

OCA at 14:44, 13 December 2023

2023-12-13T14:44:41Z

←Older revision		Revision as of 14:44, 13 December 2023
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	<StructureSection load='2je9' size='340' side='right'caption='[[2je9]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2je9' size='340' side='right'caption='[[2je9]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2je9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Diogr Diogr~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JE9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2je9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Macropsychanthus_grandiflorus Macropsychanthus grandiflorus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JE9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1dgl\|1dgl]], [[1vln\|1vln]], [[2jec\|2jec]], [[2je7\|2je7]], [[2jdz\|2jdz]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2je9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je9 OCA], [https://pdbe.org/2je9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2je9 RCSB], [https://www.ebi.ac.uk/pdbsum/2je9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2je9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2je9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je9 OCA], [https://pdbe.org/2je9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2je9 RCSB], [https://www.ebi.ac.uk/pdbsum/2je9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2je9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LECA_DIOGR LECA_DIOGR]] D-mannose/D-glucose-binding lectin. Has anti-inflammatory activity in rats. Induces histamine release in mast cells from rat. Induces lymphocyte proliferation and IFNG production. Shows toxicity against the aquatic snail B.glabrata at concentrations higher than 50 ug/ml.<ref>PMID:1398779</ref> <ref>PMID:7524287</ref> <ref>PMID:18472821</ref> <ref>PMID:9575151</ref> <ref>PMID:10747944</ref> <ref>PMID:19765980</ref>	+	[https://www.uniprot.org/uniprot/LECA_DIOGR LECA_DIOGR] D-mannose/D-glucose-binding lectin. Has anti-inflammatory activity in rats. Induces histamine release in mast cells from rat. Induces lymphocyte proliferation and IFNG production. Shows toxicity against the aquatic snail B.glabrata at concentrations higher than 50 ug/ml.<ref>PMID:1398779</ref> <ref>PMID:7524287</ref> <ref>PMID:18472821</ref> <ref>PMID:9575151</ref> <ref>PMID:10747944</ref> <ref>PMID:19765980</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Diogr]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Calvete, J J~~]]	+	[[Category: Macropsychanthus grandiflorus]]
-	[[Category: ~~Cavada, B S~~]]	+	[[Category: Calvete JJ]]
-	[[Category: ~~Nagano, C S~~]]	+	[[Category: Cavada BS]]
-	[[Category: ~~Sanz, L~~]]	+	[[Category: Nagano CS]]
-	[[Category: ~~Carbohydrate binding protein]]~~	+	[[Category: Sanz L]]
-	~~[[Category: Cona-like]]~~	+
-	~~[[Category: Legume lectin]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Sugar binding protein]]~~	+
-	~~[[Category: Sugar-binding protein~~]]	+

OCA at 11:26, 31 March 2021

2021-03-31T11:26:15Z

←Older revision		Revision as of 11:26, 31 March 2021
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	<StructureSection load='2je9' size='340' side='right'caption='[[2je9]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2je9' size='340' side='right'caption='[[2je9]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2je9]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Diogr Diogr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2JE9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2je9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Diogr Diogr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JE9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dgl\|1dgl]], [[1vln\|1vln]], [[2jec\|2jec]], [[2je7\|2je7]], [[2jdz\|2jdz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1dgl\|1dgl]], [[1vln\|1vln]], [[2jec\|2jec]], [[2je7\|2je7]], [[2jdz\|2jdz]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2je9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je9 OCA], [~~http~~://pdbe.org/2je9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2je9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2je9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2je9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2je9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je9 OCA], [https://pdbe.org/2je9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2je9 RCSB], [https://www.ebi.ac.uk/pdbsum/2je9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2je9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LECA_DIOGR LECA_DIOGR]] D-mannose/D-glucose-binding lectin. Has anti-inflammatory activity in rats. Induces histamine release in mast cells from rat. Induces lymphocyte proliferation and IFNG production. Shows toxicity against the aquatic snail B.glabrata at concentrations higher than 50 ug/ml.<ref>PMID:1398779</ref> <ref>PMID:7524287</ref> <ref>PMID:18472821</ref> <ref>PMID:9575151</ref> <ref>PMID:10747944</ref> <ref>PMID:19765980</ref>	+	[[https://www.uniprot.org/uniprot/LECA_DIOGR LECA_DIOGR]] D-mannose/D-glucose-binding lectin. Has anti-inflammatory activity in rats. Induces histamine release in mast cells from rat. Induces lymphocyte proliferation and IFNG production. Shows toxicity against the aquatic snail B.glabrata at concentrations higher than 50 ug/ml.<ref>PMID:1398779</ref> <ref>PMID:7524287</ref> <ref>PMID:18472821</ref> <ref>PMID:9575151</ref> <ref>PMID:10747944</ref> <ref>PMID:19765980</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 12:06, 10 May 2019

2019-05-10T12:06:08Z

←Older revision		Revision as of 12:06, 10 May 2019
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	==CRYSTAL STRUCTURE OF RECOMBINANT DIOCLEA GRANDIFLORA LECTIN COMPLEXED WITH 5-BROMO-4-CHLORO-3-INDOLYL-A-D-MANNOSE==		==CRYSTAL STRUCTURE OF RECOMBINANT DIOCLEA GRANDIFLORA LECTIN COMPLEXED WITH 5-BROMO-4-CHLORO-3-INDOLYL-A-D-MANNOSE==
-	<StructureSection load='2je9' size='340' side='right' caption='[[2je9]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='2je9' size='340' side='right'caption='[[2je9]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2je9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Diogr Diogr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JE9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2je9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Diogr Diogr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JE9 FirstGlance]. <br>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Diogr]]		[[Category: Diogr]]
		+	[[Category: Large Structures]]
	[[Category: Calvete, J J]]		[[Category: Calvete, J J]]
	[[Category: Cavada, B S]]		[[Category: Cavada, B S]]

OCA at 07:06, 11 July 2018

2018-07-11T07:06:05Z

←Older revision		Revision as of 07:06, 11 July 2018
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF RECOMBINANT DIOCLEA GRANDIFLORA LECTIN COMPLEXED WITH 5-BROMO-4-CHLORO-3-INDOLYL-A-D-MANNOSE==		==CRYSTAL STRUCTURE OF RECOMBINANT DIOCLEA GRANDIFLORA LECTIN COMPLEXED WITH 5-BROMO-4-CHLORO-3-INDOLYL-A-D-MANNOSE==
	<StructureSection load='2je9' size='340' side='right' caption='[[2je9]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2je9' size='340' side='right' caption='[[2je9]], [[Resolution\|resolution]] 2.10Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dgl\|1dgl]], [[1vln\|1vln]], [[2jec\|2jec]], [[2je7\|2je7]], [[2jdz\|2jdz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dgl\|1dgl]], [[1vln\|1vln]], [[2jec\|2jec]], [[2je7\|2je7]], [[2jdz\|2jdz]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2je9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je9 OCA], [http://pdbe.org/2je9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2je9 RCSB], [http://www.ebi.ac.uk/pdbsum/2je9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2je9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je9 OCA], [http://pdbe.org/2je9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2je9 RCSB], [http://www.ebi.ac.uk/pdbsum/2je9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2je9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/je/2je9_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/je/2je9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 41:		Line 42:
	[[Category: Legume lectin]]		[[Category: Legume lectin]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
		+	[[Category: Sugar binding protein]]
	[[Category: Sugar-binding protein]]		[[Category: Sugar-binding protein]]

OCA at 03:16, 9 February 2016

2016-02-09T03:16:19Z

←Older revision		Revision as of 03:16, 9 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2je9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 18:04, 10 September 2015

2015-09-10T18:04:30Z

←Older revision		Revision as of 18:04, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2je9' size='340' side='right' caption='[[2je9]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2je9' size='340' side='right' caption='[[2je9]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2je9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Dioclea_grandiflora Dioclea grandiflora~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JE9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2je9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Diogr Diogr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JE9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dgl\|1dgl]], [[1vln\|1vln]], [[2jec\|2jec]], [[2je7\|2je7]], [[2jdz\|2jdz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dgl\|1dgl]], [[1vln\|1vln]], [[2jec\|2jec]], [[2je7\|2je7]], [[2jdz\|2jdz]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2je9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2je9 RCSB], [http://www.ebi.ac.uk/pdbsum/2je9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2je9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je9 OCA], [http://pdbe.org/2je9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2je9 RCSB], [http://www.ebi.ac.uk/pdbsum/2je9 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2je9" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dioclea grandiflora~~]]	+	[[Category: Diogr]]
	[[Category: Calvete, J J]]		[[Category: Calvete, J J]]
	[[Category: Cavada, B S]]		[[Category: Cavada, B S]]

OCA at 04:02, 25 December 2014

2014-12-25T04:02:04Z

←Older revision		Revision as of 04:02, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2je9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Dioclea_grandiflora Dioclea grandiflora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JE9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2je9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Dioclea_grandiflora Dioclea grandiflora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JE9 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dgl\|1dgl]], [[1vln\|1vln]], [[2jec\|2jec]], [[2je7\|2je7]], [[2jdz\|2jdz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dgl\|1dgl]], [[1vln\|1vln]], [[2jec\|2jec]], [[2je7\|2je7]], [[2jdz\|2jdz]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2je9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2je9 RCSB], [http://www.ebi.ac.uk/pdbsum/2je9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2je9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2je9 RCSB], [http://www.ebi.ac.uk/pdbsum/2je9 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LECA_DIOGR LECA_DIOGR]] D-mannose/D-glucose-binding lectin. Has anti-inflammatory activity in rats. Induces histamine release in mast cells from rat. Induces lymphocyte proliferation and IFNG production. Shows toxicity against the aquatic snail B.glabrata at concentrations higher than 50 ug/ml.<ref>PMID:1398779</ref> <ref>PMID:7524287</ref> <ref>PMID:18472821</ref> <ref>PMID:9575151</ref> <ref>PMID:10747944</ref> <ref>PMID:19765980</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Dioclea grandiflora]]		[[Category: Dioclea grandiflora]]
-	[[Category: Calvete, J J.]]	+	[[Category: Calvete, J J]]
-	[[Category: Cavada, B S.]]	+	[[Category: Cavada, B S]]
-	[[Category: Nagano, C S.]]	+	[[Category: Nagano, C S]]
-	[[Category: Sanz, L.]]	+	[[Category: Sanz, L]]
	[[Category: Carbohydrate binding protein]]		[[Category: Carbohydrate binding protein]]
	[[Category: Cona-like]]		[[Category: Cona-like]]

OCA at 10:00, 30 September 2014

2014-09-30T10:00:14Z

←Older revision		Revision as of 10:00, 30 September 2014
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-	[[~~Image:~~2je9.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF RECOMBINANT DIOCLEA GRANDIFLORA LECTIN COMPLEXED WITH 5-BROMO-4-CHLORO-3-INDOLYL-A-D-MANNOSE==
		+	<StructureSection load='2je9' size='340' side='right' caption='[[2je9]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2je9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Dioclea_grandiflora Dioclea grandiflora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JE9 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dgl\|1dgl]], [[1vln\|1vln]], [[2jec\|2jec]], [[2je7\|2je7]], [[2jdz\|2jdz]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2je9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2je9 RCSB], [http://www.ebi.ac.uk/pdbsum/2je9 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/je/2je9_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The structural ground underlying the pH-dependency of the dimer-tetramer transition of Diocleinae lectins was investigated by equilibrium sedimentation and X-ray crystal structure determination of wild-type and site-directed mutants of recombinant lectins. Synthetic genes coding for the full-length alpha-chains of the seed lectins of Dioclea guianensis (termed r-alphaDguia) and Dioclea grandiflora (termed r-alphaDGL) were designed and expressed in Escherichia coli. This pioneering approach, which will be described in detail in the present paper, yielded recombinant lectins displaying carbohydrate-binding activity, dimer-tetramer equilibria and crystal structures indistinguishable from their natural homologues. Conversion of the pH-stable tetrameric r-alphaDGL into a structure exhibiting pH-dependent dimer-tetramer transition was accomplished through mutations that abolished the interdimeric interactions at the central cavity of the tetrameric lectins. Both the central and the peripheral interacting regions bear structural information for formation of the canonical legume lectin tetramer. We hypothesize that the strength of the ionic contacts at these sites may be modulated by the pH, leading to dissociation of those lectin structures that are not locked into a pH-stable tetramer through interdimeric contacts networking the central cavity loops.

-	~~{{STRUCTURE_2je9\| PDB=2je9 \| SCENE= }}~~	+	Insights into the structural basis of the pH-dependent dimer-tetramer equilibrium through crystallographic analysis of recombinant Diocleinae lectins.,Nagano CS, Calvete JJ, Barettino D, Perez A, Cavada BS, Sanz L Biochem J. 2008 Jan 15;409(2):417-28. PMID:17937659<ref>PMID:17937659</ref>

-	~~===CRYSTAL STRUCTURE OF RECOMBINANT DIOCLEA GRANDIFLORA LECTIN COMPLEXED WITH 5-BROMO-4-CHLORO-3-INDOLYL-A-D-MANNOSE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17937659}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2je9]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Dioclea_grandiflora Dioclea grandiflora]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2JE9 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017937659<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Dioclea grandiflora]]		[[Category: Dioclea grandiflora]]
	[[Category: Calvete, J J.]]		[[Category: Calvete, J J.]]

OCA at 14:31, 20 February 2013

2013-02-20T14:31:50Z

←Older revision		Revision as of 14:31, 20 February 2013
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	[[Image:2je9.png\|left\|200px]]		[[Image:2je9.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2je9\| PDB=2je9 \| SCENE= }}		{{STRUCTURE_2je9\| PDB=2je9 \| SCENE= }}

	===CRYSTAL STRUCTURE OF RECOMBINANT DIOCLEA GRANDIFLORA LECTIN COMPLEXED WITH 5-BROMO-4-CHLORO-3-INDOLYL-A-D-MANNOSE===		===CRYSTAL STRUCTURE OF RECOMBINANT DIOCLEA GRANDIFLORA LECTIN COMPLEXED WITH 5-BROMO-4-CHLORO-3-INDOLYL-A-D-MANNOSE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17937659}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17937659 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17937659}}		{{ABSTRACT_PUBMED_17937659}}

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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17937659~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017937659</ref><references group="xtra"/>
	[[Category: Dioclea grandiflora]]		[[Category: Dioclea grandiflora]]
	[[Category: Calvete, J J.]]		[[Category: Calvete, J J.]]
Line 28:		Line 17:
	[[Category: Nagano, C S.]]		[[Category: Nagano, C S.]]
	[[Category: Sanz, L.]]		[[Category: Sanz, L.]]
		+	[[Category: Carbohydrate binding protein]]
		+	[[Category: Cona-like]]
		+	[[Category: Legume lectin]]
		+	[[Category: Metal-binding]]
		+	[[Category: Sugar-binding protein]]

OCA: Protected "2je9" [edit=sysop:move=sysop]

2011-04-13T04:40:56Z

Protected "2je9" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:40, 13 April 2011