2ju0 - Revision history

OCA at 19:05, 29 May 2024

2024-05-29T19:05:37Z

←Older revision		Revision as of 19:05, 29 May 2024
Line 1:		Line 1:

	==Structure of Yeast Frequenin bound to PdtIns 4-kinase==		==Structure of Yeast Frequenin bound to PdtIns 4-kinase==
-	<StructureSection load='2ju0' size='340' side='right'caption='[[2ju0~~]], [[NMR_Ensembles_of_Models \| 15 NMR models~~]]' scene=''>	+	<StructureSection load='2ju0' size='340' side='right'caption='[[2ju0]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ju0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JU0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ju0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JU0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>FRQ1, NCS1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~4932 ATCC 18824]), PIK1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ju0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ju0 OCA], [https://pdbe.org/2ju0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ju0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ju0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ju0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ju0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ju0 OCA], [https://pdbe.org/2ju0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ju0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ju0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ju0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	~~[[https://www.uniprot.org/uniprot/NCS1_YEAST NCS1_YEAST]] Regulator of phosphatidylinositol 4-kinase PIK1.<ref>PMID:10559922</ref> [~~[https://www.uniprot.org/uniprot/PIK1_YEAST PIK1_YEAST]] Acts on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol 1,4,5,-trisphosphate. PIK1 is part of a nuclear phosphoinositide cycle and could control cytokinesis through the actin cytoskeleton.	+	[https://www.uniprot.org/uniprot/PIK1_YEAST PIK1_YEAST] Acts on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol 1,4,5,-trisphosphate. PIK1 is part of a nuclear phosphoinositide cycle and could control cytokinesis through the actin cytoskeleton.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 1-phosphatidylinositol 4-kinase]]
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ames, J~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Calcium]]~~	+	[[Category: Ames J]]
-	~~[[Category: Ef-hand]]~~	+
-	~~[[Category: Frequenin]]~~	+
-	~~[[Category: Lipoprotein]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Metal binding protein-signaling protein complex]]~~	+
-	~~[[Category: Myristate]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Phosphorylation]]~~	+
-	~~[[Category: Ptdins 4-kinase]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Yeast~~]]	+

OCA at 07:57, 7 April 2021

2021-04-07T07:57:05Z

←Older revision		Revision as of 07:57, 7 April 2021
Line 1:		Line 1:

	==Structure of Yeast Frequenin bound to PdtIns 4-kinase==		==Structure of Yeast Frequenin bound to PdtIns 4-kinase==
-	<StructureSection load='2ju0' size='340' side='right' caption='[[2ju0]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>	+	<StructureSection load='2ju0' size='340' side='right'caption='[[2ju0]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ju0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2JU0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ju0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JU0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRQ1, NCS1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), PIK1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRQ1, NCS1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), PIK1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ju0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ju0 OCA], [~~http~~://pdbe.org/2ju0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ju0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ju0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ju0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ju0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ju0 OCA], [https://pdbe.org/2ju0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ju0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ju0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ju0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NCS1_YEAST NCS1_YEAST]] Regulator of phosphatidylinositol 4-kinase PIK1.<ref>PMID:10559922</ref> [[~~http~~://www.uniprot.org/uniprot/PIK1_YEAST PIK1_YEAST]] Acts on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol 1,4,5,-trisphosphate. PIK1 is part of a nuclear phosphoinositide cycle and could control cytokinesis through the actin cytoskeleton.	+	[[https://www.uniprot.org/uniprot/NCS1_YEAST NCS1_YEAST]] Regulator of phosphatidylinositol 4-kinase PIK1.<ref>PMID:10559922</ref> [[https://www.uniprot.org/uniprot/PIK1_YEAST PIK1_YEAST]] Acts on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol 1,4,5,-trisphosphate. PIK1 is part of a nuclear phosphoinositide cycle and could control cytokinesis through the actin cytoskeleton.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 2ju0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2ju0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphatidylinositol 4-kinase\|Phosphatidylinositol 4-kinase]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 39:
	[[Category: 1-phosphatidylinositol 4-kinase]]		[[Category: 1-phosphatidylinositol 4-kinase]]
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Ames, J]]		[[Category: Ames, J]]
	[[Category: Calcium]]		[[Category: Calcium]]

OCA at 10:45, 18 July 2018

2018-07-18T10:45:51Z

←Older revision		Revision as of 10:45, 18 July 2018
Line 1:		Line 1:
		+
	==Structure of Yeast Frequenin bound to PdtIns 4-kinase==		==Structure of Yeast Frequenin bound to PdtIns 4-kinase==
	<StructureSection load='2ju0' size='340' side='right' caption='[[2ju0]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>		<StructureSection load='2ju0' size='340' side='right' caption='[[2ju0]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRQ1, NCS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), PIK1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRQ1, NCS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), PIK1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ju0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ju0 OCA], [http://pdbe.org/2ju0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ju0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ju0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ju0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ju0 OCA], [http://pdbe.org/2ju0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ju0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ju0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ju0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ju/2ju0_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ju/2ju0_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ju0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 36:		Line 37:
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
	[[Category: Ames, J]]		[[Category: Ames, J]]
		+	[[Category: Calcium]]
	[[Category: Ef-hand]]		[[Category: Ef-hand]]
	[[Category: Frequenin]]		[[Category: Frequenin]]

OCA at 14:35, 11 September 2015

2015-09-11T14:35:37Z

←Older revision		Revision as of 14:35, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2ju0' size='340' side='right' caption='[[2ju0]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>		<StructureSection load='2ju0' size='340' side='right' caption='[[2ju0]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ju0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JU0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ju0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JU0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRQ1, NCS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ~~Saccharomyces cerevisiae~~]), PIK1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ~~Saccharomyces cerevisiae~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRQ1, NCS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), PIK1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ju0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ju0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ju0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ju0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ju0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ju0 OCA], [http://pdbe.org/2ju0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ju0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ju0 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ju0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: 1-phosphatidylinositol 4-kinase]]		[[Category: 1-phosphatidylinositol 4-kinase]]
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
	[[Category: Ames, J]]		[[Category: Ames, J]]
	[[Category: Ef-hand]]		[[Category: Ef-hand]]

OCA at 17:49, 25 December 2014

2014-12-25T17:49:11Z

←Older revision		Revision as of 17:49, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ju0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JU0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ju0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JU0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRQ1, NCS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), PIK1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRQ1, NCS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), PIK1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ju0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ju0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ju0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ju0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ju0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ju0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ju0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ju0 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NCS1_YEAST NCS1_YEAST]] Regulator of phosphatidylinositol 4-kinase PIK1.<ref>PMID:10559922</ref> [[http://www.uniprot.org/uniprot/PIK1_YEAST PIK1_YEAST]] Acts on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol 1,4,5,-trisphosphate. PIK1 is part of a nuclear phosphoinositide cycle and could control cytokinesis through the actin cytoskeleton.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	[[Category: 1-phosphatidylinositol 4-kinase]]		[[Category: 1-phosphatidylinositol 4-kinase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Ames, J.]]	+	[[Category: Ames, J]]
	[[Category: Ef-hand]]		[[Category: Ef-hand]]
	[[Category: Frequenin]]		[[Category: Frequenin]]

OCA at 08:46, 30 September 2014

2014-09-30T08:46:26Z

←Older revision		Revision as of 08:46, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2ju0.~~png~~\|~~left~~\|200px]]	+	==Structure of Yeast Frequenin bound to PdtIns 4-kinase==
		+	<StructureSection load='2ju0' size='340' side='right' caption='[[2ju0]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ju0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JU0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRQ1, NCS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), PIK1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-phosphatidylinositol_4-kinase 1-phosphatidylinositol 4-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.67 2.7.1.67] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ju0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ju0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ju0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ju0 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ju/2ju0_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Yeast frequenin (Frq1), a small N-myristoylated EF-hand protein, activates phosphatidylinositol 4-kinase Pik1. The NMR structure of Ca2+-bound Frq1 complexed to an N-terminal Pik1 fragment (residues 121-174) was determined. The Frq1 main chain is similar to that in free Frq1 and related proteins in the same branch of the calmodulin superfamily. The myristoyl group and first eight residues of Frq1 are solvent-exposed, and Ca2+ binds the second, third, and fourth EF-hands, which associate to create a groove with two pockets. The Pik1 peptide forms two helices (125-135 and 156-169) connected by a 20-residue loop. Side chains in the Pik1 N-terminal helix (Val-127, Ala-128, Val-131, Leu-132, and Leu-135) interact with solvent-exposed residues in the Frq1 C-terminal pocket (Leu-101, Trp-103, Val-125, Leu-138, Ile-152, and Leu-155); side chains in the Pik1 C-terminal helix (Ala-157, Ala-159, Leu-160, Val-161, Met-165, and Met-167) contact solvent-exposed residues in the Frq1 N-terminal pocket (Trp-30, Phe-34, Phe-48, Ile-51, Tyr-52, Phe-55, Phe-85, and Leu-89). This defined complex confirms that residues in Pik1 pinpointed as necessary for Frq1 binding by site-directed mutagenesis are indeed sufficient for binding. Removal of the Pik1 N-terminal region (residues 8-760) from its catalytic domain (residues 792-1066) abolishes lipid kinase activity, inconsistent with Frq1 binding simply relieving an autoinhibitory constraint. Deletion of the lipid kinase unique motif (residues 35-110) also eliminates Pik1 activity. In the complex, binding of Ca2+-bound Frq1 forces the Pik1 chain into a U-turn. Frq1 may activate Pik1 by facilitating membrane targeting via the exposed N-myristoyl group and by imposing a structural transition that promotes association of the lipid kinase unique motif with the kinase domain.

-	~~{{STRUCTURE_2ju0\| PDB=2ju0 \| SCENE= }}~~	+	Structural insights into activation of phosphatidylinositol 4-kinase (Pik1) by yeast frequenin (Frq1).,Strahl T, Huttner IG, Lusin JD, Osawa M, King D, Thorner J, Ames JB J Biol Chem. 2007 Oct 19;282(42):30949-59. Epub 2007 Aug 24. PMID:17720810<ref>PMID:17720810</ref>

-	~~===Structure~~ of ~~Yeast Frequenin bound to PdtIns 4-kinase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17720810}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2ju0]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017720810<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: 1-phosphatidylinositol 4-kinase]]		[[Category: 1-phosphatidylinositol 4-kinase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]

OCA: Protected "2ju0" [edit=sysop:move=sysop]

2013-01-07T13:43:49Z

Protected "2ju0" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:43, 7 January 2013

OCA at 13:43, 7 January 2013

2013-01-07T13:43:45Z

←Older revision		Revision as of 13:43, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2ju0.png\|left\|200px]]		[[Image:2ju0.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2ju0", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2ju0\| PDB=2ju0 \| SCENE= }}		{{STRUCTURE_2ju0\| PDB=2ju0 \| SCENE= }}

	===Structure of Yeast Frequenin bound to PdtIns 4-kinase===		===Structure of Yeast Frequenin bound to PdtIns 4-kinase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17720810}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17720810 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17720810}}		{{ABSTRACT_PUBMED_17720810}}

	==About this Structure==		==About this Structure==
-	~~2JU0~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA].	+	[[2ju0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17720810~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017720810</ref><references group="xtra"/>
	[[Category: 1-phosphatidylinositol 4-kinase]]		[[Category: 1-phosphatidylinositol 4-kinase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Ames, J.]]		[[Category: Ames, J.]]
-	[[Category: Calcium]]
	[[Category: Ef-hand]]		[[Category: Ef-hand]]
	[[Category: Frequenin]]		[[Category: Frequenin]]
	[[Category: Lipoprotein]]		[[Category: Lipoprotein]]
	[[Category: Membrane]]		[[Category: Membrane]]
-	[[Category: Metal binding protein/signaling protein complex]]	+	[[Category: Metal binding protein-signaling protein complex]]
	[[Category: Myristate]]		[[Category: Myristate]]
	[[Category: Nucleus]]		[[Category: Nucleus]]
Line 39:		Line 26:
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Yeast]]		[[Category: Yeast]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:39:36 2009''

OCA at 22:39, 17 February 2009

2009-02-17T22:39:37Z

←Older revision		Revision as of 22:39, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2JU0 is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA].	+	2JU0 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA].

	==Reference==		==Reference==
-	Structural insights into activation of phosphatidylinositol 4-kinase (Pik1) by yeast frequenin (Frq1)., Strahl T, Huttner IG, Lusin JD, Osawa M, King D, Thorner J, Ames JB, J Biol Chem. 2007 Oct 19;282(42):30949-59. Epub 2007 Aug 24. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17720810 17720810]~~	+	<ref group="xtra">PMID:17720810</ref><references group="xtra"/>
	[[Category: 1-phosphatidylinositol 4-kinase]]		[[Category: 1-phosphatidylinositol 4-kinase]]
-	[[Category: Protein complex]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Ames, J.]]		[[Category: Ames, J.]]
Line 41:		Line 40:
	[[Category: Yeast]]		[[Category: Yeast]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 08~~:03:~~56 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:39:36 2009''

OCA at 05:03, 29 July 2008

2008-07-29T05:03:57Z

←Older revision		Revision as of 05:03, 29 July 2008
Line 1:		Line 1:
-	[[Image:2ju0.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2ju0.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2ju0\| PDB=2ju0 \| SCENE= }}		{{STRUCTURE_2ju0\| PDB=2ju0 \| SCENE= }}

-	~~'''~~Structure of Yeast Frequenin bound to PdtIns 4-kinase~~'''~~	+	===Structure of Yeast Frequenin bound to PdtIns 4-kinase===


-	~~==Overview==~~	+	<!--
-	~~Yeast frequenin (Frq1), a small N~~-~~myristoylated EF~~-~~hand protein~~, activates phosphatidylinositol 4-kinase Pik1. The NMR structure of Ca2+-bound Frq1 complexed to an N-terminal Pik1 fragment (residues 121-174) was determined. The Frq1 main chain is similar to that in free Frq1 and related proteins in the same branch of the calmodulin superfamily. The myristoyl group and first eight residues of Frq1 are solvent-exposed, ~~and Ca2+ binds~~ the ~~second, third, and fourth EF-hands, which associate~~ to ~~create a groove with two pockets. The Pik1 peptide forms two helices (125-135 and 156-169) connected by a 20-residue loop. Side chains in~~ the ~~Pik1 N-terminal helix~~ (Val-127, Ala-128, Val-131, Leu-132, and Leu-135) interact with solvent-exposed residues in the Frq1 C-terminal pocket (Leu-101, Trp-103, Val-125, Leu-138, Ile-152, and Leu-155); side chains in the Pik1 C-terminal helix (Ala-157, Ala-159, Leu-160, Val-161, Met-165, and Met-167) contact solvent-exposed residues in the Frq1 N-terminal pocket (Trp-30, Phe-34, Phe-48, Ile-51, Tyr-52, Phe-55, Phe-85, and Leu-89). This defined complex confirms that residues in Pik1 pinpointed as ~~necessary for Frq1 binding by site-directed mutagenesis are indeed sufficient for binding~~. ~~Removal of the Pik1 N-terminal region (residues 8-760~~) ~~from its catalytic domain (residues 792-1066) abolishes lipid kinase activity~~, ~~inconsistent with Frq1 binding simply relieving an autoinhibitory constraint. Deletion of~~ the ~~lipid kinase unique motif (residues 35-110) also eliminates Pik1 activity~~. ~~In the complex, binding of Ca2+~~-~~bound Frq1 forces the Pik1 chain into a U~~-turn. Frq1 may activate Pik1 by facilitating membrane targeting via the exposed N-myristoyl group and by imposing a structural transition that promotes association of the lipid kinase unique motif with the kinase domain.	+	The line below this paragraph, {{ABSTRACT_PUBMED_17720810}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17720810 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_17720810}}

	==About this Structure==		==About this Structure==
-	2JU0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA].	+	2JU0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JU0 OCA].

	==Reference==		==Reference==
Line 36:		Line 40:
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Yeast]]		[[Category: Yeast]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed May 7~~ 08:48:43 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:03:56 2008''