2k76 - Revision history

OCA at 12:49, 20 December 2023

2023-12-20T12:49:54Z

←Older revision		Revision as of 12:49, 20 December 2023
Line 1:		Line 1:

	==Solution structure of a paralog-specific Mena binder by NMR==		==Solution structure of a paralog-specific Mena binder by NMR==
-	<StructureSection load='2k76' size='340' side='right'caption='[[2k76~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='2k76' size='340' side='right'caption='[[2k76]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2k76]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K76 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K76 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2k76]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K76 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K76 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k76 OCA], [https://pdbe.org/2k76 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k76 RCSB], [https://www.ebi.ac.uk/pdbsum/2k76 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k76 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k76 OCA], [https://pdbe.org/2k76 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k76 RCSB], [https://www.ebi.ac.uk/pdbsum/2k76 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k76 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bayer, P]]	+	[[Category: Bayer P]]
-	[[Category: Eichler, J]]	+	[[Category: Eichler J]]
-	[[Category: Hunke, C]]	+	[[Category: Hunke C]]
-	[[Category: Link~~, N M]]~~	+	[[Category: Link NM]]
-	~~[[Category: Acta homolog]]~~	+
-	~~[[Category: App]]~~	+
-	~~[[Category: Beta-hairpin]]~~	+
-	~~[[Category: De novo protein]]~~	+
-	~~[[Category: Miniature protein]]~~	+
-	~~[[Category: Protein design~~]]	+

OCA at 07:32, 27 January 2022

2022-01-27T07:32:08Z

←Older revision		Revision as of 07:32, 27 January 2022
Line 3:		Line 3:
	<StructureSection load='2k76' size='340' side='right'caption='[[2k76]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='2k76' size='340' side='right'caption='[[2k76]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2k76]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K76 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2K76 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2k76]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K76 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K76 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2k76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k76 OCA], [~~http~~://pdbe.org/2k76 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2k76 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2k76 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2k76 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k76 OCA], [https://pdbe.org/2k76 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k76 RCSB], [https://www.ebi.ac.uk/pdbsum/2k76 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k76 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:32, 22 April 2020

2020-04-22T06:32:05Z

←Older revision		Revision as of 06:32, 22 April 2020
Line 1:		Line 1:

	==Solution structure of a paralog-specific Mena binder by NMR==		==Solution structure of a paralog-specific Mena binder by NMR==
-	<StructureSection load='2k76' size='340' side='right' caption='[[2k76]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='2k76' size='340' side='right'caption='[[2k76]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2k76]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K76 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2K76 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2k76]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K76 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2K76 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2k76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k76 OCA], [http://pdbe.org/2k76 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k76 RCSB], [http://www.ebi.ac.uk/pdbsum/2k76 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2k76 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2k76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k76 OCA], [http://pdbe.org/2k76 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k76 RCSB], [http://www.ebi.ac.uk/pdbsum/2k76 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2k76 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Bayer, P]]		[[Category: Bayer, P]]
	[[Category: Eichler, J]]		[[Category: Eichler, J]]

OCA at 10:07, 18 July 2018

2018-07-18T10:07:56Z

←Older revision		Revision as of 10:07, 18 July 2018
Line 1:		Line 1:
		+
	==Solution structure of a paralog-specific Mena binder by NMR==		==Solution structure of a paralog-specific Mena binder by NMR==
	<StructureSection load='2k76' size='340' side='right' caption='[[2k76]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='2k76' size='340' side='right' caption='[[2k76]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2k76]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K76 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K76 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2k76]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K76 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K76 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k76 OCA], [http://pdbe.org/2k76 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k76 RCSB], [http://www.ebi.ac.uk/pdbsum/2k76 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k76 OCA], [http://pdbe.org/2k76 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k76 RCSB], [http://www.ebi.ac.uk/pdbsum/2k76 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2k76 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:06, 10 September 2015

2015-09-10T16:06:37Z

←Older revision		Revision as of 16:06, 10 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2k76]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K76 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K76 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2k76]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K76 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K76 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k76 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k76 RCSB], [http://www.ebi.ac.uk/pdbsum/2k76 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k76 OCA], [http://pdbe.org/2k76 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k76 RCSB], [http://www.ebi.ac.uk/pdbsum/2k76 PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 13:		Line 13:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2k76" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:12, 19 January 2015

2015-01-19T13:12:45Z

←Older revision		Revision as of 13:12, 19 January 2015
Line 17:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bayer, P.]]	+	[[Category: Bayer, P]]
-	[[Category: Eichler, J.]]	+	[[Category: Eichler, J]]
-	[[Category: Hunke, C.]]	+	[[Category: Hunke, C]]
-	[[Category: Link, N M.]]	+	[[Category: Link, N M]]
	[[Category: Acta homolog]]		[[Category: Acta homolog]]
	[[Category: App]]		[[Category: App]]

OCA at 15:27, 12 October 2014

2014-10-12T15:27:00Z

←Older revision		Revision as of 15:27, 12 October 2014
Line 1:		Line 1:
-	[[~~Image~~:2k76.~~png\|left\|200px~~]]	+	==Solution structure of a paralog-specific Mena binder by NMR==
		+	<StructureSection load='2k76' size='340' side='right' caption='[[2k76]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2k76]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K76 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K76 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k76 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k76 RCSB], [http://www.ebi.ac.uk/pdbsum/2k76 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Abstract Ena/VASP homology 1 (EVH1) domains are polyproline binding domains that are present in a wide range of adaptor proteins, among them Ena/VASP proteins involved in actin remodeling and axonal guidance. The interaction of ActA, a transmembrane protein from the food-borne pathogen Listeria monocytogenes, with EVH1 domains has been shown to be crucial for recruitment of the host's actin skeleton and, as a consequence, for the infectivity of this bacterium. We present the structure of a synthetic high-affinity Mena EVH1 ligand, pGolemi, capable of paralog-specific binding, solved by NMR spectroscopy. This peptide shares the common pancreatic peptide fold with its scaffold, avian pancreatic peptide, but shows pivotal differences in the amino-terminus. The interplay of spatial fixation and flexibility appears to be the reason for its high affinity towards Mena EVH1. Combined with earlier investigations, our structural data shed light on the specificity determinants of pGolemi and the importance of additional binding epitopes around the residues Thr74 and Phe32 on EVH1 domains regulating paralog specificity. Our results are expected to facilitate the design of other high-affinity, paralog-specific EVH1 domain ligands, and serve as a fundament for the investigation of the molecular mode of action of EVH1 domains.

-	~~{{STRUCTURE_2k76\| PDB=2k76 \| SCENE= }}~~	+	The solution structure of pGolemi, a high affinity Mena EVH1 binding miniature protein, suggests explanations for paralog-specific binding to Ena/VASP homology (EVH) 1 domains.,Link NM, Hunke C, Mueller JW, Eichler J, Bayer P Biol Chem. 2009 May-Jun;390(5-6):417-26. PMID:19284291<ref>PMID:19284291</ref>

-	~~===Solution structure~~ of ~~a paralog-specific Mena binder by NMR===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19284291}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2k76]] is a 1 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2K76 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019284291<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bayer, P.]]		[[Category: Bayer, P.]]
	[[Category: Eichler, J.]]		[[Category: Eichler, J.]]

OCA: Protected "2k76" [edit=sysop:move=sysop]

2013-01-07T10:14:00Z

Protected "2k76" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:14, 7 January 2013

OCA at 10:13, 7 January 2013

2013-01-07T10:13:57Z

←Older revision		Revision as of 10:13, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2k76.png\|left\|200px]]		[[Image:2k76.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2k76", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2k76\| PDB=2k76 \| SCENE= }}		{{STRUCTURE_2k76\| PDB=2k76 \| SCENE= }}

	===Solution structure of a paralog-specific Mena binder by NMR===		===Solution structure of a paralog-specific Mena binder by NMR===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19284291}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19284291 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19284291}}		{{ABSTRACT_PUBMED_19284291}}

	==About this Structure==		==About this Structure==
-	~~2K76~~ is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K76 OCA].	+	[[2k76]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K76 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19284291~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019284291</ref><references group="xtra"/>
	[[Category: Bayer, P.]]		[[Category: Bayer, P.]]
	[[Category: Eichler, J.]]		[[Category: Eichler, J.]]
Line 34:		Line 22:
	[[Category: Miniature protein]]		[[Category: Miniature protein]]
	[[Category: Protein design]]		[[Category: Protein design]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 08:22:32 2009''

OCA at 05:22, 25 June 2009

2009-06-25T05:22:34Z

←Older revision		Revision as of 05:22, 25 June 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:2k76.~~jpg~~\|left\|200px]]	+	[[Image:2k76.png\|left\|200px]]

	<!--		<!--
Line 35:		Line 35:
	[[Category: Protein design]]		[[Category: Protein design]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed~~ Jun ~~17 10:~~08:04 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 25 08:22:32 2009''