2kb1 - Revision history

OCA at 09:37, 22 May 2024

2024-05-22T09:37:10Z

←Older revision		Revision as of 09:37, 22 May 2024
Line 1:		Line 1:

	==NMR studies of a channel protein without membrane: structure and dynamics of water-solubilized KcsA==		==NMR studies of a channel protein without membrane: structure and dynamics of water-solubilized KcsA==
-	<StructureSection load='2kb1' size='340' side='right'caption='[[2kb1~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2kb1' size='340' side='right'caption='[[2kb1]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kb1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KB1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kb1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KB1 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[2k1e\|2k1e]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb1 OCA], [https://pdbe.org/2kb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kb1 RCSB], [https://www.ebi.ac.uk/pdbsum/2kb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kb1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb1 OCA], [https://pdbe.org/2kb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kb1 RCSB], [https://www.ebi.ac.uk/pdbsum/2kb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kb1 ProSAT]</span></td></tr>
	</table>		</table>
Line 30:		Line 30:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Carnini, A]]	+	[[Category: Carnini A]]
-	[[Category: Eckenhoff, R]]	+	[[Category: Eckenhoff R]]
-	[[Category: Ma, D]]	+	[[Category: Ma D]]
-	[[Category: Meirovitch, E]]	+	[[Category: Meirovitch E]]
-	[[Category: Tang, P]]	+	[[Category: Tang P]]
-	[[Category: Tillman, T]]	+	[[Category: Tillman T]]
-	[[Category: Xu, Y]]	+	[[Category: Xu Y]]
-	~~[[Category: Homotetramer]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Protein~~]]	+

OCA at 07:33, 27 January 2022

2022-01-27T07:33:12Z

←Older revision		Revision as of 07:33, 27 January 2022
Line 3:		Line 3:
	<StructureSection load='2kb1' size='340' side='right'caption='[[2kb1]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kb1' size='340' side='right'caption='[[2kb1]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kb1]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2KB1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kb1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KB1 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k1e\|2k1e]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2k1e\|2k1e]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2kb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb1 OCA], [~~http~~://pdbe.org/2kb1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2kb1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2kb1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2kb1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb1 OCA], [https://pdbe.org/2kb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kb1 RCSB], [https://www.ebi.ac.uk/pdbsum/2kb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kb1 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 06:33, 22 April 2020

2020-04-22T06:33:16Z

←Older revision		Revision as of 06:33, 22 April 2020
Line 1:		Line 1:
-	~~{{Large structure}}~~	+
	==NMR studies of a channel protein without membrane: structure and dynamics of water-solubilized KcsA==		==NMR studies of a channel protein without membrane: structure and dynamics of water-solubilized KcsA==
-	<StructureSection load='2kb1' size='340' side='right' caption='[[2kb1]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2kb1' size='340' side='right'caption='[[2kb1]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kb1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB1 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2KB1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kb1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2KB1 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k1e\|2k1e]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k1e\|2k1e]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2kb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb1 OCA], [http://pdbe.org/2kb1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kb1 RCSB], [http://www.ebi.ac.uk/pdbsum/2kb1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kb1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2kb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb1 OCA], [http://pdbe.org/2kb1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kb1 RCSB], [http://www.ebi.ac.uk/pdbsum/2kb1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kb1 ProSAT]</span></td></tr>
	</table>		</table>
-	{{Large structure}}
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Carnini, A]]		[[Category: Carnini, A]]
	[[Category: Eckenhoff, R]]		[[Category: Eckenhoff, R]]

OCA at 10:18, 18 July 2018

2018-07-18T10:18:08Z

←Older revision		Revision as of 10:18, 18 July 2018
Line 1:		Line 1:
		+	{{Large structure}}
	==NMR studies of a channel protein without membrane: structure and dynamics of water-solubilized KcsA==		==NMR studies of a channel protein without membrane: structure and dynamics of water-solubilized KcsA==
	<StructureSection load='2kb1' size='340' side='right' caption='[[2kb1]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kb1' size='340' side='right' caption='[[2kb1]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2kb1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KB1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kb1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KB1 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k1e\|2k1e]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k1e\|2k1e]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb1 OCA], [http://pdbe.org/2kb1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kb1 RCSB], [http://www.ebi.ac.uk/pdbsum/2kb1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb1 OCA], [http://pdbe.org/2kb1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kb1 RCSB], [http://www.ebi.ac.uk/pdbsum/2kb1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kb1 ProSAT]</span></td></tr>
	</table>		</table>
	{{Large structure}}		{{Large structure}}
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kb/2kb1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kb/2kb1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 17:59, 8 February 2016

2016-02-08T17:59:54Z

←Older revision		Revision as of 17:59, 8 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kb1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:03, 10 September 2015

2015-09-10T14:03:09Z

←Older revision		Revision as of 14:03, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2kb1' size='340' side='right' caption='[[2kb1]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kb1' size='340' side='right' caption='[[2kb1]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kb1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KB1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kb1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KB1 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k1e\|2k1e]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k1e\|2k1e]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kb1 RCSB], [http://www.ebi.ac.uk/pdbsum/2kb1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb1 OCA], [http://pdbe.org/2kb1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kb1 RCSB], [http://www.ebi.ac.uk/pdbsum/2kb1 PDBsum]</span></td></tr>
	</table>		</table>
		+	{{Large structure}}
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2kb1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Carnini, A]]		[[Category: Carnini, A]]
	[[Category: Eckenhoff, R]]		[[Category: Eckenhoff, R]]

OCA at 14:58, 19 January 2015

2015-01-19T14:58:33Z

←Older revision		Revision as of 14:58, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2kb1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KB1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kb1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KB1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k1e\|2k1e]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k1e\|2k1e]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kb1 RCSB], [http://www.ebi.ac.uk/pdbsum/2kb1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kb1 RCSB], [http://www.ebi.ac.uk/pdbsum/2kb1 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Carnini, A.]]	+	[[Category: Carnini, A]]
-	[[Category: Eckenhoff, R.]]	+	[[Category: Eckenhoff, R]]
-	[[Category: Ma, D.]]	+	[[Category: Ma, D]]
-	[[Category: Meirovitch, E.]]	+	[[Category: Meirovitch, E]]
-	[[Category: Tang, P.]]	+	[[Category: Tang, P]]
-	[[Category: Tillman, T.]]	+	[[Category: Tillman, T]]
-	[[Category: Xu, Y.]]	+	[[Category: Xu, Y]]
	[[Category: Homotetramer]]		[[Category: Homotetramer]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Protein]]		[[Category: Protein]]

OCA at 08:31, 30 September 2014

2014-09-30T08:31:10Z

←Older revision		Revision as of 08:31, 30 September 2014
Line 1:		Line 1:
-	~~{{Large structure}}~~	+	==NMR studies of a channel protein without membrane: structure and dynamics of water-solubilized KcsA==
-	~~{{STRUCTURE_2kb1\| PDB=2kb1 \| SCENE= }}~~	+	<StructureSection load='2kb1' size='340' side='right' caption='[[2kb1]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	===NMR studies of a channel protein without membrane: structure and dynamics of water-solubilized KcsA===	+	== Structural highlights ==
-	~~{{ABSTRACT_PUBMED_18948596}}~~	+	<table><tr><td colspan='2'>[[2kb1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KB1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k1e\|2k1e]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kb1 RCSB], [http://www.ebi.ac.uk/pdbsum/2kb1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kb/2kb1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Structural studies of polytopic membrane proteins are often hampered by the vagaries of these proteins in membrane mimetic environments and by the difficulties in handling them with conventional techniques. Designing and creating water-soluble analogues with preserved native structures offer an attractive alternative. We report here solution NMR studies of WSK3, a water-soluble analogue of the potassium channel KcsA. The WSK3 NMR structure (PDB ID code 2K1E) resembles the KcsA crystal structures, validating the approach. By more stringent comparison criteria, however, the introduction of several charged residues aimed at improving water solubility seems to have led to the possible formations of a few salt bridges and hydrogen bonds not present in the native structure, resulting in slight differences in the structure of WSK3 relative to KcsA. NMR dynamics measurements show that WSK3 is highly flexible in the absence of a lipid environment. Reduced spectral density mapping and model-free analyses reveal dynamic characteristics consistent with an isotropically tumbling tetramer experiencing slow (nanosecond) motions with unusually low local ordering. An altered hydrogen-bond network near the selectivity filter and the pore helix, and the intrinsically dynamic nature of the selectivity filter, support the notion that this region is crucial for slow inactivation. Our results have implications not only for the design of water-soluble analogues of membrane proteins but also for our understanding of the basic determinants of intrinsic protein structure and dynamics.

-	~~==About this Structure==~~	+	NMR studies of a channel protein without membranes: structure and dynamics of water-solubilized KcsA.,Ma D, Tillman TS, Tang P, Meirovitch E, Eckenhoff R, Carnini A, Xu Y Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16537-42. Epub 2008 Oct 23. PMID:18948596<ref>PMID:18948596</ref>
-	~~[[2kb1]] is~~ a ~~4 chain~~ structure ~~with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2KB1 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	<~~ref group="xtra">PMID:018948596<~~/~~ref~~><~~references group="xtra"~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Carnini, A.]]		[[Category: Carnini, A.]]

OCA at 07:46, 11 March 2013

2013-03-11T07:46:14Z

←Older revision		Revision as of 07:46, 11 March 2013
Line 1:		Line 1:
-	~~[[Image:2kb1.png\|left\|200px]]~~	+	{{Large structure}}
-		+
	{{STRUCTURE_2kb1\| PDB=2kb1 \| SCENE= }}		{{STRUCTURE_2kb1\| PDB=2kb1 \| SCENE= }}
-
	===NMR studies of a channel protein without membrane: structure and dynamics of water-solubilized KcsA===		===NMR studies of a channel protein without membrane: structure and dynamics of water-solubilized KcsA===
-
	{{ABSTRACT_PUBMED_18948596}}		{{ABSTRACT_PUBMED_18948596}}

OCA: Protected "2kb1" [edit=sysop:move=sysop]

2013-01-07T13:31:30Z

Protected "2kb1" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:31, 7 January 2013