2kgd - Revision history

OCA at 08:21, 7 April 2021

2021-04-07T08:21:16Z

←Older revision		Revision as of 08:21, 7 April 2021
Line 1:		Line 1:

	==NMR Solution Structures of 3-oxo-butyl-ACP, an intermediate mimic from the actinorhodin polyketide synthase in Streptomyces coelicolor==		==NMR Solution Structures of 3-oxo-butyl-ACP, an intermediate mimic from the actinorhodin polyketide synthase in Streptomyces coelicolor==
-	<StructureSection load='2kgd' size='340' side='right' caption='[[2kgd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2kgd' size='340' side='right'caption='[[2kgd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kgd]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2KGD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kgd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KGD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SXV:N~3~-[(2S)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-N-{2-[(3-OXOBUTYL)SULFANYL]ETHYL}-BETA-ALANINAMIDE'>SXV</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SXV:N~3~-[(2S)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-N-{2-[(3-OXOBUTYL)SULFANYL]ETHYL}-BETA-ALANINAMIDE'>SXV</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k0x\|2k0x]], [[2k0y\|2k0y]], [[2kg6\|2kg6]], [[2kg8\|2kg8]], [[2kg9\|2kg9]], [[2kga\|2kga]], [[2kgc\|2kgc]], [[2kge\|2kge]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2k0x\|2k0x]], [[2k0y\|2k0y]], [[2kg6\|2kg6]], [[2kg8\|2kg8]], [[2kg9\|2kg9]], [[2kga\|2kga]], [[2kgc\|2kgc]], [[2kge\|2kge]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCO5089, SCBAC28G1.15 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCO5089, SCBAC28G1.15 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2kgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kgd OCA], [~~http~~://pdbe.org/2kgd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2kgd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2kgd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2kgd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kgd OCA], [https://pdbe.org/2kgd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kgd RCSB], [https://www.ebi.ac.uk/pdbsum/2kgd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kgd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ACPX_STRCO ACPX_STRCO]] Acyl carrier protein.	+	[[https://www.uniprot.org/uniprot/ACPX_STRCO ACPX_STRCO]] Acyl carrier protein.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]	+	*[[Acyl carrier protein synthase 3D structures\|Acyl carrier protein synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Crump, M P]]		[[Category: Crump, M P]]
	[[Category: Evans, S E]]		[[Category: Evans, S E]]

OCA at 10:33, 18 July 2018

2018-07-18T10:33:10Z

←Older revision		Revision as of 10:33, 18 July 2018
Line 1:		Line 1:
		+
	==NMR Solution Structures of 3-oxo-butyl-ACP, an intermediate mimic from the actinorhodin polyketide synthase in Streptomyces coelicolor==		==NMR Solution Structures of 3-oxo-butyl-ACP, an intermediate mimic from the actinorhodin polyketide synthase in Streptomyces coelicolor==
	<StructureSection load='2kgd' size='340' side='right' caption='[[2kgd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kgd' size='340' side='right' caption='[[2kgd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k0x\|2k0x]], [[2k0y\|2k0y]], [[2kg6\|2kg6]], [[2kg8\|2kg8]], [[2kg9\|2kg9]], [[2kga\|2kga]], [[2kgc\|2kgc]], [[2kge\|2kge]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k0x\|2k0x]], [[2k0y\|2k0y]], [[2kg6\|2kg6]], [[2kg8\|2kg8]], [[2kg9\|2kg9]], [[2kga\|2kga]], [[2kgc\|2kgc]], [[2kge\|2kge]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCO5089, SCBAC28G1.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCO5089, SCBAC28G1.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kgd OCA], [http://pdbe.org/2kgd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kgd RCSB], [http://www.ebi.ac.uk/pdbsum/2kgd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kgd OCA], [http://pdbe.org/2kgd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kgd RCSB], [http://www.ebi.ac.uk/pdbsum/2kgd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kgd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kg/2kgd_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kg/2kgd_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 14:42, 8 February 2016

2016-02-08T14:42:20Z

←Older revision		Revision as of 14:42, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kgd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:35, 10 September 2015

2015-09-10T12:35:57Z

←Older revision		Revision as of 12:35, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2kgd' size='340' side='right' caption='[[2kgd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kgd' size='340' side='right' caption='[[2kgd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kgd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_coelicolor Streptomyces~~ coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KGD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kgd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KGD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SXV:N~3~-[(2S)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-N-{2-[(3-OXOBUTYL)SULFANYL]ETHYL}-BETA-ALANINAMIDE'>SXV</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SXV:N~3~-[(2S)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-N-{2-[(3-OXOBUTYL)SULFANYL]ETHYL}-BETA-ALANINAMIDE'>SXV</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k0x\|2k0x]], [[2k0y\|2k0y]], [[2kg6\|2kg6]], [[2kg8\|2kg8]], [[2kg9\|2kg9]], [[2kga\|2kga]], [[2kgc\|2kgc]], [[2kge\|2kge]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k0x\|2k0x]], [[2k0y\|2k0y]], [[2kg6\|2kg6]], [[2kg8\|2kg8]], [[2kg9\|2kg9]], [[2kga\|2kga]], [[2kgc\|2kgc]], [[2kge\|2kge]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCO5089, SCBAC28G1.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 ~~Streptomyces~~ coelicolor])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCO5089, SCBAC28G1.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kgd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kgd RCSB], [http://www.ebi.ac.uk/pdbsum/2kgd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kgd OCA], [http://pdbe.org/2kgd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kgd RCSB], [http://www.ebi.ac.uk/pdbsum/2kgd PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2kgd" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptomyces coelicolor]]
	[[Category: Crump, M P]]		[[Category: Crump, M P]]
	[[Category: Evans, S E]]		[[Category: Evans, S E]]

OCA at 00:05, 25 December 2014

2014-12-25T00:05:27Z

←Older revision		Revision as of 00:05, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2kgd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KGD FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kgd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KGD FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SXV:N~3~-[(2S)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-N-{2-[(3-OXOBUTYL)SULFANYL]ETHYL}-BETA-ALANINAMIDE'>SXV</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SXV:N~3~-[(2S)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-N-{2-[(3-OXOBUTYL)SULFANYL]ETHYL}-BETA-ALANINAMIDE'>SXV</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k0x\|2k0x]], [[2k0y\|2k0y]], [[2kg6\|2kg6]], [[2kg8\|2kg8]], [[2kg9\|2kg9]], [[2kga\|2kga]], [[2kgc\|2kgc]], [[2kge\|2kge]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k0x\|2k0x]], [[2k0y\|2k0y]], [[2kg6\|2kg6]], [[2kg8\|2kg8]], [[2kg9\|2kg9]], [[2kga\|2kga]], [[2kgc\|2kgc]], [[2kge\|2kge]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCO5089, SCBAC28G1.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCO5089, SCBAC28G1.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kgd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kgd RCSB], [http://www.ebi.ac.uk/pdbsum/2kgd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kgd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kgd RCSB], [http://www.ebi.ac.uk/pdbsum/2kgd PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACPX_STRCO ACPX_STRCO]] Acyl carrier protein.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
-	[[Category: Crump, M P.]]	+	[[Category: Crump, M P]]
-	[[Category: Evans, S E.]]	+	[[Category: Evans, S E]]
-	[[Category: Williams, C.]]	+	[[Category: Williams, C]]
	[[Category: Acp]]		[[Category: Acp]]
	[[Category: Actinorhodin]]		[[Category: Actinorhodin]]

OCA at 04:00, 29 September 2014

2014-09-29T04:00:32Z

←Older revision		Revision as of 04:00, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2kgd.~~png~~\|~~left~~\|200px]]	+	==NMR Solution Structures of 3-oxo-butyl-ACP, an intermediate mimic from the actinorhodin polyketide synthase in Streptomyces coelicolor==
		+	<StructureSection load='2kgd' size='340' side='right' caption='[[2kgd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2kgd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KGD FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SXV:N~3~-[(2S)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-N-{2-[(3-OXOBUTYL)SULFANYL]ETHYL}-BETA-ALANINAMIDE'>SXV</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2k0x\|2k0x]], [[2k0y\|2k0y]], [[2kg6\|2kg6]], [[2kg8\|2kg8]], [[2kg9\|2kg9]], [[2kga\|2kga]], [[2kgc\|2kgc]], [[2kge\|2kge]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCO5089, SCBAC28G1.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kgd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kgd RCSB], [http://www.ebi.ac.uk/pdbsum/2kgd PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kg/2kgd_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acyl carrier proteins (ACPs) are essential to both fatty acid synthase (FAS) and polyketide synthase (PKS) biosynthetic pathways, yet relatively little is known about how they function at a molecular level. Seven thiol ester and thiol ether derivatives of the actinorhodin (act) PKS ACP from Streptomyces coelicolor have been prepared and structurally characterised by NMR to gain insight into ACP-intermediate interactions. Holo ACP synthase has been used to prepare early-stage ACP intermediates of polyketide biosynthesis (holo ACP, acetyl ACP, and malonyl ACP) from the respective coenzyme A derivatives. A synthetic route to stabilised thiol ether ACPs was developed and applied to the preparation of stable 3-oxobutyl and 3,5-dioxohexyl ACP as diketide and triketide analogues. No interaction between the protein and the acyl phosphopantetheine moieties of acetyl, malonyl, or 3-oxobutyl ACP was detected. Analysis of (1)H-(15)N heteronuclear single quantum coherence and nuclear Overhauser enhancement spectroscopy spectra for the triketide ACP revealed exchange between a major ('Tri', 85%) and a minor protein conformer in which the polyketide interacts with the protein ('Tri()', 15%). Act ACP was also derivatised with butyryl, hexanoyl, and octanoyl groups. The corresponding NMR spectra showed large chemical shift perturbations centred on helices II and III, indicative of acyl chain binding and significant structural rearrangement. Unexpectedly, butyryl act ACP showed almost identical backbone (1)H-(15)N chemical shifts to Tri(), suggesting comparable structural changes that might provide insight into the structurally uncharacterised polyketide bound form. Furthermore, butyryl ACP itself underwent slow conformational exchange with a second minor conformer (But(*)) with almost identical backbone chemical shifts to octanoyl act ACP. High-resolution NMR structures of these acylated forms revealed that act ACP was able to undergo dramatic conformational changes that exceed those seen in FAS ACPs. When compared to E. coli FAS ACP, the substrate binding pocket of the act PKS ACP has three specific amino acid substitutions (Thr39/Leu45, Ala68/Leu74, and Leu42/Thr48) that alter the size, shape, and location of this cavity. These conformational changes may play a role in protein-protein recognition and assist the binding of bulky polyketide intermediates.

-	~~{{STRUCTURE_2kgd\| PDB=2kgd \| SCENE= }}~~	+	Probing the Interactions of early polyketide intermediates with the Actinorhodin ACP from S. coelicolor A3(2).,Evans SE, Williams C, Arthur CJ, Ploskon E, Wattana-amorn P, Cox RJ, Crosby J, Willis CL, Simpson TJ, Crump MP J Mol Biol. 2009 Jun 12;389(3):511-28. Epub 2009 Apr 8. PMID:19361520<ref>PMID:19361520</ref>

-	~~===NMR Solution Structures~~ of ~~3-oxo-butyl-ACP, an intermediate mimic from~~ the ~~actinorhodin polyketide synthase in Streptomyces coelicolor===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19361520}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2kgd]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA].~~	+

	==See Also==		==See Also==
	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]		*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019361520<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Crump, M P.]]		[[Category: Crump, M P.]]

OCA at 10:29, 20 October 2012

2012-10-20T10:29:35Z

←Older revision		Revision as of 10:29, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[2kgd]] is a 1 chain structure ~~of [[Acyl carrier protein synthase]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA].	+	[[2kgd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA].

	==See Also==		==See Also==

OCA: Protected "2kgd" [edit=sysop:move=sysop]

2012-07-26T02:18:10Z

Protected "2kgd" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:18, 26 July 2012

OCA at 02:18, 26 July 2012

2012-07-26T02:18:09Z

←Older revision		Revision as of 02:18, 26 July 2012
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-	{{Seed}}
	[[Image:2kgd.png\|left\|200px]]		[[Image:2kgd.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2kgd", creates the "Structure Box" on the page.
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-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_2kgd\| PDB=2kgd \| SCENE= }}		{{STRUCTURE_2kgd\| PDB=2kgd \| SCENE= }}

	===NMR Solution Structures of 3-oxo-butyl-ACP, an intermediate mimic from the actinorhodin polyketide synthase in Streptomyces coelicolor===		===NMR Solution Structures of 3-oxo-butyl-ACP, an intermediate mimic from the actinorhodin polyketide synthase in Streptomyces coelicolor===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19361520}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19361520 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19361520}}		{{ABSTRACT_PUBMED_19361520}}

	==About this Structure==		==About this Structure==
-	~~2KGD~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA].	+	[[2kgd]] is a 1 chain structure of [[Acyl carrier protein synthase]] with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA].
		+
		+	==See Also==
		+	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19361520~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019361520</ref><references group="xtra"/>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Crump, M P.]]		[[Category: Crump, M P.]]
Line 36:		Line 27:
	[[Category: Phosphopantetheine]]		[[Category: Phosphopantetheine]]
	[[Category: Polyketide mimic]]		[[Category: Polyketide mimic]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 10 22:40:54 2009''

OCA at 19:40, 10 June 2009

2009-06-10T19:40:54Z

←Older revision		Revision as of 19:40, 10 June 2009
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	===NMR Solution Structures of 3-oxo-butyl-ACP, an intermediate mimic from the actinorhodin polyketide synthase in Streptomyces coelicolor===		===NMR Solution Structures of 3-oxo-butyl-ACP, an intermediate mimic from the actinorhodin polyketide synthase in Streptomyces coelicolor===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19361520}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19361520 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19361520}}

	==About this Structure==		==About this Structure==
	2KGD is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA].		2KGD is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGD OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19361520</ref><references group="xtra"/>
	[[Category: Streptomyces coelicolor]]		[[Category: Streptomyces coelicolor]]
	[[Category: Crump, M P.]]		[[Category: Crump, M P.]]
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	[[Category: Polyketide mimic]]		[[Category: Polyketide mimic]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 10 21:06:45 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 10 22:40:54 2009''