2koh - Revision history

OCA at 09:40, 22 May 2024

2024-05-22T09:40:49Z

←Older revision		Revision as of 09:40, 22 May 2024
Line 1:		Line 1:

	==NMR structure of mouse Par3-PDZ3 in complex with VE-Cadherin C-terminus==		==NMR structure of mouse Par3-PDZ3 in complex with VE-Cadherin C-terminus==
-	<StructureSection load='2koh' size='340' side='right'caption='[[2koh~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2koh' size='340' side='right'caption='[[2koh]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2koh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KOH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2koh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KOH FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Par3, Pard3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~10090 LK3 transgenic mice]), Cdh5, VE-Cadherin ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2koh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2koh OCA], [https://pdbe.org/2koh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2koh RCSB], [https://www.ebi.ac.uk/pdbsum/2koh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2koh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2koh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2koh OCA], [https://pdbe.org/2koh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2koh RCSB], [https://www.ebi.ac.uk/pdbsum/2koh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2koh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PARD3_MOUSE PARD3_MOUSE]] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Involved in Schwann cell peripheral myelination. [[https://www.uniprot.org/uniprot/CADH5_MOUSE CADH5_MOUSE]] Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.<ref>PMID:9220534</ref> <ref>PMID:20332120</ref>	+	[https://www.uniprot.org/uniprot/PARD3_MOUSE PARD3_MOUSE] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Involved in Schwann cell peripheral myelination.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Peterson~~, F C~~]]	+	[[Category: Peterson FC]]
-	[[Category: Tyler~~, R C~~]]	+	[[Category: Tyler RC]]
-	[[Category: Volkman~~, B F]]~~	+	[[Category: Volkman BF]]
-	~~[[Category: Alternative splicing]]~~	+
-	~~[[Category: Calcium]]~~	+
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Cell division]]~~	+
-	~~[[Category: Cell junction]]~~	+
-	~~[[Category: Cell membrane]]~~	+
-	~~[[Category: Cleavage on pair of basic residue]]~~	+
-	~~[[Category: Coiled coil]]~~	+
-	~~[[Category: Cytoplasm]]~~	+
-	~~[[Category: Cytoskeleton]]~~	+
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Par3]]~~	+
-	~~[[Category: Pdz domain]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Tight junction]]~~	+
-	~~[[Category: Transmembrane]]~~	+
-	~~[[Category: Ve cadherin~~]]	+

OCA at 07:29, 14 April 2021

2021-04-14T07:29:31Z

←Older revision		Revision as of 07:29, 14 April 2021
Line 1:		Line 1:

	==NMR structure of mouse Par3-PDZ3 in complex with VE-Cadherin C-terminus==		==NMR structure of mouse Par3-PDZ3 in complex with VE-Cadherin C-terminus==
-	<StructureSection load='2koh' size='340' side='right' caption='[[2koh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2koh' size='340' side='right'caption='[[2koh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2koh]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2KOH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2koh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KOH FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Par3, Pard3 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Cdh5, VE-Cadherin ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Par3, Pard3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Cdh5, VE-Cadherin ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2koh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2koh OCA], [~~http~~://pdbe.org/2koh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2koh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2koh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2koh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2koh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2koh OCA], [https://pdbe.org/2koh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2koh RCSB], [https://www.ebi.ac.uk/pdbsum/2koh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2koh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PARD3_MOUSE PARD3_MOUSE]] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Involved in Schwann cell peripheral myelination. [[~~http~~://www.uniprot.org/uniprot/CADH5_MOUSE CADH5_MOUSE]] Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.<ref>PMID:9220534</ref> <ref>PMID:20332120</ref>	+	[[https://www.uniprot.org/uniprot/PARD3_MOUSE PARD3_MOUSE]] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Involved in Schwann cell peripheral myelination. [[https://www.uniprot.org/uniprot/CADH5_MOUSE CADH5_MOUSE]] Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.<ref>PMID:9220534</ref> <ref>PMID:20332120</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Cadherin\|Cadherin]]	+	*[[Cadherin 3D structures\|Cadherin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Peterson, F C]]		[[Category: Peterson, F C]]

OCA at 07:51, 25 July 2018

2018-07-25T07:51:13Z

←Older revision		Revision as of 07:51, 25 July 2018
Line 1:		Line 1:
		+
	==NMR structure of mouse Par3-PDZ3 in complex with VE-Cadherin C-terminus==		==NMR structure of mouse Par3-PDZ3 in complex with VE-Cadherin C-terminus==
	<StructureSection load='2koh' size='340' side='right' caption='[[2koh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2koh' size='340' side='right' caption='[[2koh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2koh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2koh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOH FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Par3, Pard3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Cdh5, VE-Cadherin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Par3, Pard3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Cdh5, VE-Cadherin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2koh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2koh OCA], [http://pdbe.org/2koh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2koh RCSB], [http://www.ebi.ac.uk/pdbsum/2koh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2koh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2koh OCA], [http://pdbe.org/2koh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2koh RCSB], [http://www.ebi.ac.uk/pdbsum/2koh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2koh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ko/2koh_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ko/2koh_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 38:		Line 39:
	[[Category: Tyler, R C]]		[[Category: Tyler, R C]]
	[[Category: Volkman, B F]]		[[Category: Volkman, B F]]
		+	[[Category: Alternative splicing]]
		+	[[Category: Calcium]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
Line 44:		Line 47:
	[[Category: Cell membrane]]		[[Category: Cell membrane]]
	[[Category: Cleavage on pair of basic residue]]		[[Category: Cleavage on pair of basic residue]]
		+	[[Category: Coiled coil]]
		+	[[Category: Cytoplasm]]
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]
	[[Category: Glycoprotein]]		[[Category: Glycoprotein]]

OCA at 05:30, 8 February 2016

2016-02-08T05:30:33Z

←Older revision		Revision as of 05:30, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2koh ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:22, 10 September 2015

2015-09-10T08:22:30Z

←Older revision		Revision as of 08:22, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2koh' size='340' side='right' caption='[[2koh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2koh' size='340' side='right' caption='[[2koh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2koh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2koh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOH FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Par3, Pard3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~]), Cdh5, VE-Cadherin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Par3, Pard3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Cdh5, VE-Cadherin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2koh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2koh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2koh RCSB], [http://www.ebi.ac.uk/pdbsum/2koh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2koh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2koh OCA], [http://pdbe.org/2koh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2koh RCSB], [http://www.ebi.ac.uk/pdbsum/2koh PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2koh" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Peterson, F C]]		[[Category: Peterson, F C]]
	[[Category: Tyler, R C]]		[[Category: Tyler, R C]]

OCA at 20:58, 24 December 2014

2014-12-24T20:58:38Z

←Older revision		Revision as of 20:58, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2koh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2koh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOH FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Par3, Pard3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), Cdh5, VE-Cadherin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Par3, Pard3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), Cdh5, VE-Cadherin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2koh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2koh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2koh RCSB], [http://www.ebi.ac.uk/pdbsum/2koh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2koh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2koh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2koh RCSB], [http://www.ebi.ac.uk/pdbsum/2koh PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PARD3_MOUSE PARD3_MOUSE]] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Involved in Schwann cell peripheral myelination. [[http://www.uniprot.org/uniprot/CADH5_MOUSE CADH5_MOUSE]] Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.<ref>PMID:9220534</ref> <ref>PMID:20332120</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Peterson, F C.]]	+	[[Category: Peterson, F C]]
-	[[Category: Tyler, R C.]]	+	[[Category: Tyler, R C]]
-	[[Category: Volkman, B F.]]	+	[[Category: Volkman, B F]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]

OCA at 05:31, 29 September 2014

2014-09-29T05:31:06Z

←Older revision		Revision as of 05:31, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2koh.~~png\|left~~\|200px]]	+	==NMR structure of mouse Par3-PDZ3 in complex with VE-Cadherin C-terminus==
		+	<StructureSection load='2koh' size='340' side='right' caption='[[2koh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2koh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOH FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Par3, Pard3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), Cdh5, VE-Cadherin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2koh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2koh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2koh RCSB], [http://www.ebi.ac.uk/pdbsum/2koh PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ko/2koh_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	par3 is a multiple-PDZ-containing scaffold protein that is central to the organization of an evolutionarily conserved cell polarity complex consisting of par3, par6, and aPKC. The ability of par3 PDZ domains to target various adhesion molecules and enzymes at the plasma membrane leads to the controlled localization of par6 and aPKC, which has firmly established its role in epithelial cell polarity. Of the numerous PDZ ligands associated with par3, interaction of its third PDZ domain with the class II ligand found within the C-terminal tail of vascular endothelial cadherin (VE-Cad) suggests a role in endothelial cell polarity as well, but the molecular details of the interaction are unknown. Previously determined structures of par3-PDZ3 bound to the class I ligand found within the C-terminal tail of the phosphoinositide phosphatase PTEN revealed two discrete binding sites: a canonical PDZ-ligand interaction site and a distal site involving charge-charge complements. Currently, it is unclear if par3-PDZ3 employs both canonical and distal binding modes in its association with VE-Cad or if these modes are unique to the PTEN interaction, suggesting a possible mechanism for ligand specificity within the polarity network. The structure of par3-PDZ3 bound to the C-terminal tail of VE-Cad presented in this work shows that both canonical and distal interactions are utilized in binding. Biophysical measurements using fluorescence polarization and two-dimensional NMR implicate the intermolecular charge pairing of aspartic acid 777 (VE-Cad) and arginine 609 (par3-PDZ3) as a crucial modulator of complex formation. Phosphorylation of VE-Cad at serine 776 increases its affinity for par3, demonstrating that post-translational modifications outside of the canonical carboxylate binding site can enhance PDZ-ligand interactions. Comparison of the VE-Cad and PTEN complexes highlights how the unique molecular architecture of par3-PDZ3 can accommodate both canonical and distal interaction modes that allow dual-class specificity for these two ligand types.

-	~~{{STRUCTURE_2koh\| PDB=2koh \| SCENE= }}~~	+	Distal interactions within the par3-VE-cadherin complex.,Tyler RC, Peterson FC, Volkman BF Biochemistry. 2010 Feb 9;49(5):951-7. PMID:20047332<ref>PMID:20047332</ref>

-	~~===NMR structure~~ of ~~mouse Par3-PDZ3 in complex with VE-Cadherin C-terminus===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20047332}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2koh]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA].~~	+

	==See Also==		==See Also==
	*[[Cadherin\|Cadherin]]		*[[Cadherin\|Cadherin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020047332<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Peterson, F C.]]		[[Category: Peterson, F C.]]

OCA at 09:04, 20 October 2012

2012-10-20T09:04:30Z

←Older revision		Revision as of 09:04, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2koh]] is a 2 chain structure ~~of [[Cadherin]]~~ with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA].	+	[[2koh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOH OCA].

	==See Also==		==See Also==

OCA at 21:41, 25 July 2012

2012-07-25T21:41:35Z

←Older revision		Revision as of 21:41, 25 July 2012
Line 1:		Line 1:
	[[Image:2koh.png\|left\|200px]]		[[Image:2koh.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2koh", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2koh\| PDB=2koh \| SCENE= }}		{{STRUCTURE_2koh\| PDB=2koh \| SCENE= }}

	===NMR structure of mouse Par3-PDZ3 in complex with VE-Cadherin C-terminus===		===NMR structure of mouse Par3-PDZ3 in complex with VE-Cadherin C-terminus===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20047332}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20047332 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20047332}}		{{ABSTRACT_PUBMED_20047332}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Cadherin]]	+	*[[Cadherin\|Cadherin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20047332~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020047332</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Peterson, F C.]]		[[Category: Peterson, F C.]]
	[[Category: Tyler, R C.]]		[[Category: Tyler, R C.]]
	[[Category: Volkman, B F.]]		[[Category: Volkman, B F.]]
-	[[Category: Alternative splicing]]
-	[[Category: Calcium]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
Line 38:		Line 25:
	[[Category: Cell membrane]]		[[Category: Cell membrane]]
	[[Category: Cleavage on pair of basic residue]]		[[Category: Cleavage on pair of basic residue]]
-	[[Category: Coiled coil]]
-	[[Category: Cytoplasm]]
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]
	[[Category: Glycoprotein]]		[[Category: Glycoprotein]]

OCA: Protected "2koh" [edit=sysop:move=sysop]

2010-12-27T02:16:28Z

Protected "2koh" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:16, 27 December 2010