2l04 - Revision history

OCA at 06:50, 1 May 2024

2024-05-01T06:50:56Z

←Older revision		Revision as of 06:50, 1 May 2024
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	==The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein==		==The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein==
-	<StructureSection load='2l04' size='340' side='right'caption='[[2l04~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2l04' size='340' side='right'caption='[[2l04]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l04]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bacteriophage_lambda Bacteriophage lambda~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L04 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2l04]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L04 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>V ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~10710 Bacteriophage lambda])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l04 OCA], [https://pdbe.org/2l04 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l04 RCSB], [https://www.ebi.ac.uk/pdbsum/2l04 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l04 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l04 OCA], [https://pdbe.org/2l04 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l04 RCSB], [https://www.ebi.ac.uk/pdbsum/2l04 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l04 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~VMTV_LAMBD VMTV_LAMBD~~]~~] Protein forming~~ the phage's tail tube~~, which is~~ composed of ~~about~~ 32 hexameric disks. ~~There are 135-212 copies~~ of ~~gene V protein per mature phage~~. ~~Probably undergoes structural rearrangements leading to injection of~~ the ~~phage DNA into the host (By similarity)~~.	+	[https://www.uniprot.org/uniprot/TUBE_LAMBD TUBE_LAMBD] Forms the phage's tail tube composed of 32 hexameric disks. When it encounters the appropriate initiation complex gpM and gpL, it assembles in hexameric rings that stack on top of each others. Multimerization ceases when the correct tail length is achieved through a mechanism dependent on tail terminator protein.<ref>PMID:2150582</ref> <ref>PMID:23911548</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2l04 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2l04 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Immunoglobulin (Ig)-like domains are found frequently on the surface of tailed double-stranded DNA bacteriophages, yet their functional role remains obscure. Here, we have investigated the structure and function of the C-terminal Ig-like domain of gpV (gpV(C)), the tail tube protein of phage lambda. This domain has been predicted through sequence similarity to be a member of the bacterial Ig-like domain 2 (Big_2) family, which is composed of more than 1300 phage and bacterial sequences. Using trypsin proteolysis, we have delineated the boundaries of gpV(C) and have shown that its removal reduces the biological activity of gpV by 100-fold; thus providing a definitive demonstration of a functional role for this domain. Determination of the solution structure of gpV(C) by NMR spectroscopy showed that it adopts a canonical Ig-like fold of the I-set class. This represents the first structure of a phage-encoded Ig-like domain and only the second structure of a Big_2 domain. Structural and sequence comparisons indicate that the gpV(C) structure is more representative of both the phage-encoded Big_2 domains and Big_2 domains in general than the other available Big_2 structure. Bioinformatics analyses have identified two conserved clusters of residues on the surface of gpV(C) that may be important in mediating the function of this domain.
-
-	The Solution Structure of the C-Terminal Ig-like Domain of the Bacteriophage lambda Tail Tube Protein.,Pell LG, Gasmi-Seabrook GM, Morais M, Neudecker P, Kanelis V, Bona D, Donaldson LW, Edwards AM, Howell PL, Davidson AR, Maxwell KL J Mol Biol. 2010 Sep 6. PMID:20826161<ref>PMID:20826161</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2l04" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacteriophage lambda~~]]	+	[[Category: Escherichia virus Lambda]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Davidson~~, A R~~]]	+	[[Category: Davidson AR]]
-	[[Category: Donaldson~~, L W~~]]	+	[[Category: Donaldson LW]]
-	[[Category: Gasmi-Seabrook~~, G M.C~~]]	+	[[Category: Gasmi-Seabrook GMC]]
-	[[Category: Howell, P]]	+	[[Category: Howell P]]
-	[[Category: Maxwell~~, K L~~]]	+	[[Category: Maxwell KL]]
-	[[Category: Pell~~, L G]]~~	+	[[Category: Pell LG]]
-	~~[[Category: Gpv]]~~	+
-	~~[[Category: Ig-like domain]]~~	+
-	~~[[Category: Phage tail]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 08:32, 10 November 2021

2021-11-10T08:32:02Z

←Older revision		Revision as of 08:32, 10 November 2021
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	<StructureSection load='2l04' size='340' side='right'caption='[[2l04]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2l04' size='340' side='right'caption='[[2l04]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l04]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2L04 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2l04]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L04 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">V ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">V ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2l04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l04 OCA], [~~http~~://pdbe.org/2l04 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2l04 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2l04 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2l04 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l04 OCA], [https://pdbe.org/2l04 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l04 RCSB], [https://www.ebi.ac.uk/pdbsum/2l04 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l04 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/VMTV_LAMBD VMTV_LAMBD]] Protein forming the phage's tail tube, which is composed of about 32 hexameric disks. There are 135-212 copies of gene V protein per mature phage. Probably undergoes structural rearrangements leading to injection of the phage DNA into the host (By similarity).	+	[[https://www.uniprot.org/uniprot/VMTV_LAMBD VMTV_LAMBD]] Protein forming the phage's tail tube, which is composed of about 32 hexameric disks. There are 135-212 copies of gene V protein per mature phage. Probably undergoes structural rearrangements leading to injection of the phage DNA into the host (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:29, 5 February 2020

2020-02-05T08:29:57Z

←Older revision		Revision as of 08:29, 5 February 2020
Line 1:		Line 1:

	==The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein==		==The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein==
-	<StructureSection load='2l04' size='340' side='right' caption='[[2l04]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2l04' size='340' side='right'caption='[[2l04]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2l04]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L04 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2l04]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L04 FirstGlance]. <br>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Bacteriophage lambda]]		[[Category: Bacteriophage lambda]]
		+	[[Category: Large Structures]]
	[[Category: Davidson, A R]]		[[Category: Davidson, A R]]
	[[Category: Donaldson, L W]]		[[Category: Donaldson, L W]]

OCA at 08:18, 25 July 2018

2018-07-25T08:18:56Z

←Older revision		Revision as of 08:18, 25 July 2018
Line 1:		Line 1:
		+
	==The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein==		==The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein==
	<StructureSection load='2l04' size='340' side='right' caption='[[2l04]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2l04' size='340' side='right' caption='[[2l04]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2l04]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L04 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2l04]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L04 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">V ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">V ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l04 OCA], [http://pdbe.org/2l04 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l04 RCSB], [http://www.ebi.ac.uk/pdbsum/2l04 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l04 OCA], [http://pdbe.org/2l04 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l04 RCSB], [http://www.ebi.ac.uk/pdbsum/2l04 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l04 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l0/2l04_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l0/2l04_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2l04 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:45, 10 September 2015

2015-09-10T05:45:48Z

←Older revision		Revision as of 05:45, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2l04' size='340' side='right' caption='[[2l04]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2l04' size='340' side='right' caption='[[2l04]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l04]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_lambda Enterobacteria phage~~ lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L04 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2l04]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L04 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">V ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 ~~Enterobacteria phage~~ lambda])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">V ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l04 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l04 RCSB], [http://www.ebi.ac.uk/pdbsum/2l04 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l04 OCA], [http://pdbe.org/2l04 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l04 RCSB], [http://www.ebi.ac.uk/pdbsum/2l04 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2l04" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacteria phage~~ lambda]]	+	[[Category: Bacteriophage lambda]]
	[[Category: Davidson, A R]]		[[Category: Davidson, A R]]
	[[Category: Donaldson, L W]]		[[Category: Donaldson, L W]]
Line 37:		Line 38:
	[[Category: Maxwell, K L]]		[[Category: Maxwell, K L]]
	[[Category: Pell, L G]]		[[Category: Pell, L G]]
-	[[Category: Bacteriophage lambda]]
	[[Category: Gpv]]		[[Category: Gpv]]
	[[Category: Ig-like domain]]		[[Category: Ig-like domain]]
	[[Category: Phage tail]]		[[Category: Phage tail]]
	[[Category: Viral protein]]		[[Category: Viral protein]]

OCA at 18:52, 24 December 2014

2014-12-24T18:52:57Z

←Older revision		Revision as of 18:52, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l04 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l04 RCSB], [http://www.ebi.ac.uk/pdbsum/2l04 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l04 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l04 RCSB], [http://www.ebi.ac.uk/pdbsum/2l04 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/VMTV_LAMBD VMTV_LAMBD]] Protein forming the phage's tail tube, which is composed of about 32 hexameric disks. There are 135-212 copies of gene V protein per mature phage. Probably undergoes structural rearrangements leading to injection of the phage DNA into the host (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 12:27, 18 December 2014

2014-12-18T12:27:29Z

←Older revision		Revision as of 12:27, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2l04\| PDB=2l04 \| SCENE= }}~~	+	==The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein==
-	===The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein===	+	<StructureSection load='2l04' size='340' side='right' caption='[[2l04]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~{{ABSTRACT_PUBMED_20826161}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2l04]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L04 FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">V ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l04 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l04 RCSB], [http://www.ebi.ac.uk/pdbsum/2l04 PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l0/2l04_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Immunoglobulin (Ig)-like domains are found frequently on the surface of tailed double-stranded DNA bacteriophages, yet their functional role remains obscure. Here, we have investigated the structure and function of the C-terminal Ig-like domain of gpV (gpV(C)), the tail tube protein of phage lambda. This domain has been predicted through sequence similarity to be a member of the bacterial Ig-like domain 2 (Big_2) family, which is composed of more than 1300 phage and bacterial sequences. Using trypsin proteolysis, we have delineated the boundaries of gpV(C) and have shown that its removal reduces the biological activity of gpV by 100-fold; thus providing a definitive demonstration of a functional role for this domain. Determination of the solution structure of gpV(C) by NMR spectroscopy showed that it adopts a canonical Ig-like fold of the I-set class. This represents the first structure of a phage-encoded Ig-like domain and only the second structure of a Big_2 domain. Structural and sequence comparisons indicate that the gpV(C) structure is more representative of both the phage-encoded Big_2 domains and Big_2 domains in general than the other available Big_2 structure. Bioinformatics analyses have identified two conserved clusters of residues on the surface of gpV(C) that may be important in mediating the function of this domain.

-	~~==Function==~~	+	The Solution Structure of the C-Terminal Ig-like Domain of the Bacteriophage lambda Tail Tube Protein.,Pell LG, Gasmi-Seabrook GM, Morais M, Neudecker P, Kanelis V, Bona D, Donaldson LW, Edwards AM, Howell PL, Davidson AR, Maxwell KL J Mol Biol. 2010 Sep 6. PMID:20826161<ref>PMID:20826161</ref>
-	~~[[http://www.uniprot.org/uniprot/VMTV_LAMBD VMTV_LAMBD]] Protein forming~~ the ~~phage's tail tube, which is composed~~ of ~~about 32 hexameric disks~~. ~~There are 135~~-~~212 copies of gene~~ V ~~protein per mature phage~~. ~~Probably undergoes structural rearrangements leading to injection of the phage DNA into the host (By similarity)~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[2l04]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:020826161</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Enterobacteria phage lambda]]		[[Category: Enterobacteria phage lambda]]
-	[[Category: Davidson, A R.]]	+	[[Category: Davidson, A R]]
-	[[Category: Donaldson, L W.]]	+	[[Category: Donaldson, L W]]
-	[[Category: Gasmi-Seabrook, G M.C.]]	+	[[Category: Gasmi-Seabrook, G M.C]]
-	[[Category: Howell, P.]]	+	[[Category: Howell, P]]
-	[[Category: Maxwell, K L.]]	+	[[Category: Maxwell, K L]]
-	[[Category: Pell, L G.]]	+	[[Category: Pell, L G]]
	[[Category: Bacteriophage lambda]]		[[Category: Bacteriophage lambda]]
	[[Category: Gpv]]		[[Category: Gpv]]

OCA at 08:28, 19 June 2013

2013-06-19T08:28:49Z

←Older revision		Revision as of 08:28, 19 June 2013
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-	[[Image:2l04.png\|left\|200px]]
-
	{{STRUCTURE_2l04\| PDB=2l04 \| SCENE= }}		{{STRUCTURE_2l04\| PDB=2l04 \| SCENE= }}
-
	===The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein===		===The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein===
		+	{{ABSTRACT_PUBMED_20826161}}

-	~~{{ABSTRACT_PUBMED_20826161}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/VMTV_LAMBD VMTV_LAMBD]] Protein forming the phage's tail tube, which is composed of about 32 hexameric disks. There are 135-212 copies of gene V protein per mature phage. Probably undergoes structural rearrangements leading to injection of the phage DNA into the host (By similarity).

	==About this Structure==		==About this Structure==
Line 11:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:020826161</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020826161</ref><references group="xtra"/><references/>
	[[Category: Enterobacteria phage lambda]]		[[Category: Enterobacteria phage lambda]]
	[[Category: Davidson, A R.]]		[[Category: Davidson, A R.]]

OCA: Protected "2l04" [edit=sysop:move=sysop]

2013-01-07T11:53:01Z

Protected "2l04" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:53, 7 January 2013

OCA at 11:52, 7 January 2013

2013-01-07T11:52:55Z

←Older revision		Revision as of 11:52, 7 January 2013
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-	~~{{Seed}}~~	+	[[Image:2l04.png\|left\|200px]]
-	[[Image:2l04.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2l04", creates the "Structure Box" on the page.
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	{{STRUCTURE_2l04\| PDB=2l04 \| SCENE= }}		{{STRUCTURE_2l04\| PDB=2l04 \| SCENE= }}

	===The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein===		===The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20826161}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20826161 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_20826161}}		{{ABSTRACT_PUBMED_20826161}}

	==About this Structure==		==About this Structure==
-	~~2L04~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA].	+	[[2l04]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L04 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20826161~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020826161</ref><references group="xtra"/>
	[[Category: Enterobacteria phage lambda]]		[[Category: Enterobacteria phage lambda]]
	[[Category: Davidson, A R.]]		[[Category: Davidson, A R.]]
Line 36:		Line 24:
	[[Category: Phage tail]]		[[Category: Phage tail]]
	[[Category: Viral protein]]		[[Category: Viral protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 14:41:37 2010''