2leu - Revision history

OCA at 12:52, 20 December 2023

2023-12-20T12:52:16Z

←Older revision		Revision as of 12:52, 20 December 2023
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	==HIGH RESOLUTION 1H NMR STUDY OF LEUCOCIN A IN 90% AQUEOUS TRIFLUOROETHANOL (TFE) (0.1% TFA), 18 STRUCTURES==		==HIGH RESOLUTION 1H NMR STUDY OF LEUCOCIN A IN 90% AQUEOUS TRIFLUOROETHANOL (TFE) (0.1% TFA), 18 STRUCTURES==
-	<StructureSection load='2leu' size='340' side='right'caption='[[2leu~~]], [[NMR_Ensembles_of_Models \| 18 NMR models~~]]' scene=''>	+	<StructureSection load='2leu' size='340' side='right'caption='[[2leu]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2leu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LEU FirstGlance]. <br>		<table><tr><td colspan='2'>[[2leu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LEU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2leu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2leu OCA], [https://pdbe.org/2leu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2leu RCSB], [https://www.ebi.ac.uk/pdbsum/2leu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2leu ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2leu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2leu OCA], [https://pdbe.org/2leu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2leu RCSB], [https://www.ebi.ac.uk/pdbsum/2leu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2leu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LCCA_LEUGE LCCA_LEUGE]] Inhibits a wide spectrum of lactic acid bacteria.	+	[https://www.uniprot.org/uniprot/LCCA_LEUGE LCCA_LEUGE] Inhibits a wide spectrum of lactic acid bacteria.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 24:
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Leuconostoc gelidum]]		[[Category: Leuconostoc gelidum]]
-	[[Category: Gallagher~~, N L.F~~]]	+	[[Category: Gallagher NLF]]
-	[[Category: Nakashima~~, T T~~]]	+	[[Category: Nakashima TT]]
-	[[Category: Niemczura~~, W P~~]]	+	[[Category: Niemczura WP]]
-	[[Category: Sailer, M]]	+	[[Category: Sailer M]]
-	[[Category: Stiles~~, M E~~]]	+	[[Category: Stiles ME]]
-	[[Category: Vederas~~, J C]]~~	+	[[Category: Vederas JC]]
-	~~[[Category: Antibacterial peptide]]~~	+
-	~~[[Category: Bacteriocin~~]]	+

OCA at 07:51, 9 February 2022

2022-02-09T07:51:13Z

←Older revision		Revision as of 07:51, 9 February 2022
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	<StructureSection load='2leu' size='340' side='right'caption='[[2leu]], [[NMR_Ensembles_of_Models \| 18 NMR models]]' scene=''>		<StructureSection load='2leu' size='340' side='right'caption='[[2leu]], [[NMR_Ensembles_of_Models \| 18 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2leu]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2LEU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2leu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LEU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2leu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2leu OCA], [~~http~~://pdbe.org/2leu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2leu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2leu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2leu ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2leu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2leu OCA], [https://pdbe.org/2leu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2leu RCSB], [https://www.ebi.ac.uk/pdbsum/2leu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2leu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LCCA_LEUGE LCCA_LEUGE]] Inhibits a wide spectrum of lactic acid bacteria.	+	[[https://www.uniprot.org/uniprot/LCCA_LEUGE LCCA_LEUGE]] Inhibits a wide spectrum of lactic acid bacteria.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:03, 6 May 2020

2020-05-06T07:03:24Z

←Older revision		Revision as of 07:03, 6 May 2020
Line 1:		Line 1:

	==HIGH RESOLUTION 1H NMR STUDY OF LEUCOCIN A IN 90% AQUEOUS TRIFLUOROETHANOL (TFE) (0.1% TFA), 18 STRUCTURES==		==HIGH RESOLUTION 1H NMR STUDY OF LEUCOCIN A IN 90% AQUEOUS TRIFLUOROETHANOL (TFE) (0.1% TFA), 18 STRUCTURES==
-	<StructureSection load='2leu' size='340' side='right' caption='[[2leu]], [[NMR_Ensembles_of_Models \| 18 NMR models]]' scene=''>	+	<StructureSection load='2leu' size='340' side='right'caption='[[2leu]], [[NMR_Ensembles_of_Models \| 18 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2leu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2LEU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2leu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2LEU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2leu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2leu OCA], [http://pdbe.org/2leu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2leu RCSB], [http://www.ebi.ac.uk/pdbsum/2leu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2leu ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2leu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2leu OCA], [http://pdbe.org/2leu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2leu RCSB], [http://www.ebi.ac.uk/pdbsum/2leu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2leu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Leuconostoc gelidum]]		[[Category: Leuconostoc gelidum]]
	[[Category: Gallagher, N L.F]]		[[Category: Gallagher, N L.F]]

OCA at 19:32, 1 August 2018

2018-08-01T19:32:14Z

←Older revision		Revision as of 19:32, 1 August 2018
Line 1:		Line 1:
		+
	==HIGH RESOLUTION 1H NMR STUDY OF LEUCOCIN A IN 90% AQUEOUS TRIFLUOROETHANOL (TFE) (0.1% TFA), 18 STRUCTURES==		==HIGH RESOLUTION 1H NMR STUDY OF LEUCOCIN A IN 90% AQUEOUS TRIFLUOROETHANOL (TFE) (0.1% TFA), 18 STRUCTURES==
	<StructureSection load='2leu' size='340' side='right' caption='[[2leu]], [[NMR_Ensembles_of_Models \| 18 NMR models]]' scene=''>		<StructureSection load='2leu' size='340' side='right' caption='[[2leu]], [[NMR_Ensembles_of_Models \| 18 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2leu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LEU FirstGlance]. <br>		<table><tr><td colspan='2'>[[2leu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LEU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2leu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2leu OCA], [http://pdbe.org/2leu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2leu RCSB], [http://www.ebi.ac.uk/pdbsum/2leu PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2leu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2leu OCA], [http://pdbe.org/2leu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2leu RCSB], [http://www.ebi.ac.uk/pdbsum/2leu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2leu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 09:52, 11 September 2015

2015-09-11T09:52:10Z

←Older revision		Revision as of 09:52, 11 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2leu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LEU FirstGlance]. <br>		<table><tr><td colspan='2'>[[2leu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LEU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2leu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2leu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2leu RCSB], [http://www.ebi.ac.uk/pdbsum/2leu PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2leu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2leu OCA], [http://pdbe.org/2leu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2leu RCSB], [http://www.ebi.ac.uk/pdbsum/2leu PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2leu" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:46, 25 December 2014

2014-12-25T14:46:00Z

←Older revision		Revision as of 14:46, 25 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2leu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2leu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2leu RCSB], [http://www.ebi.ac.uk/pdbsum/2leu PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2leu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2leu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2leu RCSB], [http://www.ebi.ac.uk/pdbsum/2leu PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LCCA_LEUGE LCCA_LEUGE]] Inhibits a wide spectrum of lactic acid bacteria.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Leuconostoc gelidum]]		[[Category: Leuconostoc gelidum]]
-	[[Category: Gallagher, N L.F.]]	+	[[Category: Gallagher, N L.F]]
-	[[Category: Nakashima, T T.]]	+	[[Category: Nakashima, T T]]
-	[[Category: Niemczura, W P.]]	+	[[Category: Niemczura, W P]]
-	[[Category: Sailer, M.]]	+	[[Category: Sailer, M]]
-	[[Category: Stiles, M E.]]	+	[[Category: Stiles, M E]]
-	[[Category: Vederas, J C.]]	+	[[Category: Vederas, J C]]
	[[Category: Antibacterial peptide]]		[[Category: Antibacterial peptide]]
	[[Category: Bacteriocin]]		[[Category: Bacteriocin]]

OCA at 11:28, 20 October 2014

2014-10-20T11:28:17Z

←Older revision		Revision as of 11:28, 20 October 2014
Line 1:		Line 1:
-	[[~~Image~~:2leu.~~png\|left\|200px~~]]	+	==HIGH RESOLUTION 1H NMR STUDY OF LEUCOCIN A IN 90% AQUEOUS TRIFLUOROETHANOL (TFE) (0.1% TFA), 18 STRUCTURES==
		+	<StructureSection load='2leu' size='340' side='right' caption='[[2leu]], [[NMR_Ensembles_of_Models \| 18 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2leu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LEU FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2leu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2leu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2leu RCSB], [http://www.ebi.ac.uk/pdbsum/2leu PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The first three-dimensional structure of a type IIa bacteriocin from lactic acid bacteria is reported. Complete 1H resonance assignments of leucocin A, a 37 amino acid antimicrobial peptide isolated from the lactic acid bacterium Leuconostoc gelidum UAL187, were determined in 90% trifluoroethanol (TFE)-water and in aqueous dodecylphosphocholine (DPC) micelles (1:40 ratio of leucocin A:DPC) using two-dimensional NMR techniques (e.g., DQF-COSY, TOCSY, NOESY). Circular dichroism spectra, NMR chemical shift indices, amide hydrogen exchange rates, and long-range nuclear Overhauser effects indicate that leucocin A adopts a reasonably well defined structure in both TFE and DPC micelle environments but exists as a random coil in water or aqueous DMSO. Distance geometry and simulated annealing calculations were employed to generate structures for leucocin A in both lipophilic media. While some differences were noted between the structures calculated for the two different solvent systems, in both, the region encompassing residues 17-31 assumes an essentially identical amphiphilic alpha-helix conformation. A three-strand antiparallel beta-sheet domain (residues 2-16), anchored by the disulfide bridge, is also observed in both media. In TFE, these two regions have a more defined relationship relative to each other, while, in DPC micelles, the C-terminus is folded back onto the alpha-helix. The implications of these structural features with regard to the antimicrobial mechanism of action and target recognition are discussed.

-	~~{{STRUCTURE_2leu\| PDB=2leu \| SCENE= }}~~	+	Three-dimensional structure of leucocin A in trifluoroethanol and dodecylphosphocholine micelles: spatial location of residues critical for biological activity in type IIa bacteriocins from lactic acid bacteria.,Fregeau Gallagher NL, Sailer M, Niemczura WP, Nakashima TT, Stiles ME, Vederas JC Biochemistry. 1997 Dec 9;36(49):15062-72. PMID:9398233<ref>PMID:9398233</ref>

-	~~===HIGH RESOLUTION 1H NMR STUDY OF LEUCOCIN A IN 90% AQUEOUS TRIFLUOROETHANOL (TFE) (0~~.~~1% TFA), 18 STRUCTURES===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9398233}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[2leu]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA].~~	+	</StructureSection>
	[[Category: Leuconostoc gelidum]]		[[Category: Leuconostoc gelidum]]
	[[Category: Gallagher, N L.F.]]		[[Category: Gallagher, N L.F.]]

OCA: Protected "2leu" [edit=sysop:move=sysop]

2013-01-07T14:58:52Z

Protected "2leu" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:58, 7 January 2013

OCA at 14:58, 7 January 2013

2013-01-07T14:58:48Z

←Older revision		Revision as of 14:58, 7 January 2013
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-	{{Seed}}
	[[Image:2leu.png\|left\|200px]]		[[Image:2leu.png\|left\|200px]]

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	{{STRUCTURE_2leu\| PDB=2leu \| SCENE= }}		{{STRUCTURE_2leu\| PDB=2leu \| SCENE= }}

	===HIGH RESOLUTION 1H NMR STUDY OF LEUCOCIN A IN 90% AQUEOUS TRIFLUOROETHANOL (TFE) (0.1% TFA), 18 STRUCTURES===		===HIGH RESOLUTION 1H NMR STUDY OF LEUCOCIN A IN 90% AQUEOUS TRIFLUOROETHANOL (TFE) (0.1% TFA), 18 STRUCTURES===

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	{{ABSTRACT_PUBMED_9398233}}		{{ABSTRACT_PUBMED_9398233}}

	==About this Structure==		==About this Structure==
-	~~2LEU is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA].	+	[[2leu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA].
-		+
-	~~==Reference==~~	+
-	Three-dimensional structure of leucocin A in trifluoroethanol and dodecylphosphocholine micelles: spatial location of residues critical for biological activity in type IIa bacteriocins from lactic acid bacteria., Fregeau Gallagher NL, Sailer M, Niemczura WP, Nakashima TT, Stiles ME, Vederas JC, Biochemistry. 1997 Dec 9;36(49):15062-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9398233 9398233]	+
	[[Category: Leuconostoc gelidum]]		[[Category: Leuconostoc gelidum]]
-	[[Category: Single protein]]
	[[Category: Gallagher, N L.F.]]		[[Category: Gallagher, N L.F.]]
	[[Category: Nakashima, T T.]]		[[Category: Nakashima, T T.]]
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	[[Category: Antibacterial peptide]]		[[Category: Antibacterial peptide]]
	[[Category: Bacteriocin]]		[[Category: Bacteriocin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:10:26 2008''

OCA at 00:10, 29 July 2008

2008-07-29T00:10:30Z

←Older revision		Revision as of 00:10, 29 July 2008
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-	[[Image:2leu.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2leu.png\|left\|200px]]

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	{{STRUCTURE_2leu\| PDB=2leu \| SCENE= }}		{{STRUCTURE_2leu\| PDB=2leu \| SCENE= }}

-	~~'''~~HIGH RESOLUTION 1H NMR STUDY OF LEUCOCIN A IN 90% AQUEOUS TRIFLUOROETHANOL (TFE) (0.1% TFA), 18 STRUCTURES~~'''~~	+	===HIGH RESOLUTION 1H NMR STUDY OF LEUCOCIN A IN 90% AQUEOUS TRIFLUOROETHANOL (TFE) (0.1% TFA), 18 STRUCTURES===


-	~~==Overview==~~	+	<!--
-	The ~~first three-dimensional structure of a type IIa bacteriocin from lactic acid bacteria is reported. Complete 1H resonance assignments of leucocin A~~, ~~a 37 amino acid antimicrobial peptide isolated from~~ the ~~lactic acid bacterium Leuconostoc gelidum UAL187, were determined in 90% trifluoroethanol~~ (~~TFE)-water and in aqueous dodecylphosphocholine (DPC) micelles (1~~:~~40 ratio of leucocin A:DPC) using two-dimensional NMR techniques (e~~.g.~~, DQF-COSY, TOCSY, NOESY~~)~~. Circular dichroism spectra~~, NMR chemical shift indices, amide hydrogen exchange rates, and long-range nuclear Overhauser effects indicate that leucocin A adopts a reasonably well defined structure in both TFE and DPC micelle environments but exists as a random coil in water or aqueous DMSO. Distance geometry and simulated annealing calculations were employed to generate structures for leucocin A in both lipophilic media. While some differences were noted between the ~~structures calculated for the two different solvent systems, in both, the region encompassing residues 17-31 assumes an essentially identical amphiphilic alpha-helix conformation~~. ~~A three~~-~~strand antiparallel beta~~-sheet domain (residues 2-16), anchored by the disulfide bridge, is also observed in both media. In TFE, these two regions have a more defined relationship relative to each other, while, in DPC micelles, the C-terminus is folded back onto the alpha-helix. The implications of these structural features with regard to the antimicrobial mechanism of action and target recognition are discussed.	+	The line below this paragraph, {{ABSTRACT_PUBMED_9398233}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 9398233 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_9398233}}

	==About this Structure==		==About this Structure==
-	2LEU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA].	+	2LEU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Leuconostoc_gelidum Leuconostoc gelidum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LEU OCA].

	==Reference==		==Reference==
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	[[Category: Antibacterial peptide]]		[[Category: Antibacterial peptide]]
	[[Category: Bacteriocin]]		[[Category: Bacteriocin]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 09~~:30:05 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:10:26 2008''