2lnh - Revision history

OCA at 06:56, 1 May 2024

2024-05-01T06:56:37Z

←Older revision		Revision as of 06:56, 1 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LNH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LNH FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [https://pdbe.org/2lnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [https://www.ebi.ac.uk/pdbsum/2lnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lnh ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [https://pdbe.org/2lnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [https://www.ebi.ac.uk/pdbsum/2lnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lnh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/WASL_HUMAN WASL_HUMAN] Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.<ref>PMID:19366662</ref>		[https://www.uniprot.org/uniprot/WASL_HUMAN WASL_HUMAN] Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.<ref>PMID:19366662</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Intrinsically disordered protein (IDP)-mediated interactions are often characterized by low affinity but high specificity. These traits are essential in signaling and regulation that require reversibility. Enterohaemorrhagic Escherichia coli (EHEC) exploit this situation by commandeering host cytoskeletal signaling to stimulate actin assembly beneath bound bacteria, generating "pedestals" that promote intestinal colonization. EHEC translocates two proteins, EspF(U) and Tir, which form a complex with the host protein IRTKS. The interaction of this complex with N-WASP triggers localized actin polymerization. We show that EspF(U) is an IDP that contains a transiently alpha-helical N-terminus and dynamic C-terminus. Our structure shows that single EspF(U) repeat forms a high-affinity trimolecular complex with N-WASP and IRTKS. We demonstrate that bacterial and cellular ligands interact with IRTKS SH3 in a similar fashion, but the bacterial protein has evolved to outcompete cellular targets by utilizing a tryptophan switch that offers superior binding affinity enabling EHEC-induced pedestal formation.
-
-	Enterohaemorrhagic Escherichia Coli Exploits a Tryptophan Switch to Hijack Host F-Actin Assembly.,Aitio O, Hellman M, Skehan B, Kesti T, Leong JM, Saksela K, Permi P Structure. 2012 Aug 21. PMID:22921828<ref>PMID:22921828</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2lnh" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[Wiskott-Aldrich syndrome protein\|Wiskott-Aldrich syndrome protein]]	+	*[[Wiskott-Aldrich syndrome protein 3D structures\|Wiskott-Aldrich syndrome protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:09, 15 February 2023

2023-02-15T11:09:07Z

←Older revision		Revision as of 11:09, 15 February 2023
Line 1:		Line 1:

	==Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly==		==Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly==
-	<StructureSection load='2lnh' size='340' side='right'caption='[[2lnh~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2lnh' size='340' side='right'caption='[[2lnh]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Eco57 Eco57~~] and [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LNH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LNH FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WASL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), BAIAP2L1, IRTKS ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), espF(U), tccP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [https://pdbe.org/2lnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [https://www.ebi.ac.uk/pdbsum/2lnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lnh ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [https://pdbe.org/2lnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [https://www.ebi.ac.uk/pdbsum/2lnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lnh ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/WASL_HUMAN WASL_HUMAN]] Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.<ref>PMID:19366662</ref> [[https://www.uniprot.org/uniprot/ESFU3_ECO57 ESFU3_ECO57]] Required for efficient pedestal formation in host epithelial cells during infection. Acts as an intermediate between Tir (via host BAIAP2) and host WASL/N-WASP. Directly binds and activates WASL/N-WASP, which stimulates actin polymerization and leads to the formation of actin pedestals at the sites of bacterial adhesion.<ref>PMID:18650809</ref> <ref>PMID:22921828</ref> [[https://www.uniprot.org/uniprot/BI2L1_HUMAN BI2L1_HUMAN]] May function as adapter protein. Involved in the formation of clusters of actin bundles. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection.<ref>PMID:17430976</ref> <ref>PMID:19366662</ref> <ref>PMID:22921828</ref>	+	[https://www.uniprot.org/uniprot/WASL_HUMAN WASL_HUMAN] Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.<ref>PMID:19366662</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Eco57~~]]	+	[[Category: Escherichia coli O157:H7]]
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Aitio, O]]	+	[[Category: Aitio O]]
-	[[Category: Hellman, M]]	+	[[Category: Hellman M]]
-	[[Category: Kesti, T]]	+	[[Category: Kesti T]]
-	[[Category: Leong~~, J M~~]]	+	[[Category: Leong JM]]
-	[[Category: Permi, P]]	+	[[Category: Permi P]]
-	[[Category: Saksela, K]]	+	[[Category: Saksela K]]
-	[[Category: Skehan, B]]	+	[[Category: Skehan B]]
-	~~[[Category: Protein complex]]~~	+
-	~~[[Category: Signaling protein-protein binding complex~~]]	+

OCA at 10:14, 12 May 2021

2021-05-12T10:14:23Z

←Older revision		Revision as of 10:14, 12 May 2021
Line 1:		Line 1:

	==Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly==		==Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly==
-	<StructureSection load='2lnh' size='340' side='right' caption='[[2lnh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2lnh' size='340' side='right'caption='[[2lnh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Eco57 Eco57] and [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2LNH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Eco57 Eco57] and [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LNH FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WASL ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), BAIAP2L1, IRTKS ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), espF(U), tccP ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WASL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), BAIAP2L1, IRTKS ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), espF(U), tccP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [~~http~~://pdbe.org/2lnh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2lnh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2lnh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [https://pdbe.org/2lnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [https://www.ebi.ac.uk/pdbsum/2lnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lnh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/WASL_HUMAN WASL_HUMAN]] Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.<ref>PMID:19366662</ref> [[~~http~~://www.uniprot.org/uniprot/ESFU3_ECO57 ESFU3_ECO57]] Required for efficient pedestal formation in host epithelial cells during infection. Acts as an intermediate between Tir (via host BAIAP2) and host WASL/N-WASP. Directly binds and activates WASL/N-WASP, which stimulates actin polymerization and leads to the formation of actin pedestals at the sites of bacterial adhesion.<ref>PMID:18650809</ref> <ref>PMID:22921828</ref> [[~~http~~://www.uniprot.org/uniprot/BI2L1_HUMAN BI2L1_HUMAN]] May function as adapter protein. Involved in the formation of clusters of actin bundles. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection.<ref>PMID:17430976</ref> <ref>PMID:19366662</ref> <ref>PMID:22921828</ref>	+	[[https://www.uniprot.org/uniprot/WASL_HUMAN WASL_HUMAN]] Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.<ref>PMID:19366662</ref> [[https://www.uniprot.org/uniprot/ESFU3_ECO57 ESFU3_ECO57]] Required for efficient pedestal formation in host epithelial cells during infection. Acts as an intermediate between Tir (via host BAIAP2) and host WASL/N-WASP. Directly binds and activates WASL/N-WASP, which stimulates actin polymerization and leads to the formation of actin pedestals at the sites of bacterial adhesion.<ref>PMID:18650809</ref> <ref>PMID:22921828</ref> [[https://www.uniprot.org/uniprot/BI2L1_HUMAN BI2L1_HUMAN]] May function as adapter protein. Involved in the formation of clusters of actin bundles. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection.<ref>PMID:17430976</ref> <ref>PMID:19366662</ref> <ref>PMID:22921828</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 27:
	[[Category: Eco57]]		[[Category: Eco57]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Aitio, O]]		[[Category: Aitio, O]]
	[[Category: Hellman, M]]		[[Category: Hellman, M]]

OCA at 19:25, 1 August 2018

2018-08-01T19:25:29Z

←Older revision		Revision as of 19:25, 1 August 2018
Line 1:		Line 1:
		+
	==Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly==		==Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly==
	<StructureSection load='2lnh' size='340' side='right' caption='[[2lnh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2lnh' size='340' side='right' caption='[[2lnh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57] and [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LNH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57] and [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LNH FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WASL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), BAIAP2L1, IRTKS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), espF(U), tccP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WASL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), BAIAP2L1, IRTKS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), espF(U), tccP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [http://pdbe.org/2lnh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [http://www.ebi.ac.uk/pdbsum/2lnh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [http://pdbe.org/2lnh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [http://www.ebi.ac.uk/pdbsum/2lnh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lnh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 20:44, 10 September 2015

2015-09-10T20:44:15Z

←Older revision		Revision as of 20:44, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2lnh' size='340' side='right' caption='[[2lnh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2lnh' size='340' side='right' caption='[[2lnh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_o157:h7 Escherichia coli o157:h7~~] and [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LNH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57] and [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LNH FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WASL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), BAIAP2L1, IRTKS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), espF(U), tccP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ~~Escherichia coli O157:H7~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WASL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), BAIAP2L1, IRTKS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), espF(U), tccP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [http://www.ebi.ac.uk/pdbsum/2lnh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [http://pdbe.org/2lnh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [http://www.ebi.ac.uk/pdbsum/2lnh PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2lnh" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Wiskott-Aldrich syndrome protein\|Wiskott-Aldrich syndrome protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli o157:h7~~]]	+	[[Category: Eco57]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Aitio, O]]		[[Category: Aitio, O]]
	[[Category: Hellman, M]]		[[Category: Hellman, M]]

OCA at 05:55, 25 December 2014

2014-12-25T05:55:16Z

←Older revision		Revision as of 05:55, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LNH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LNH FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WASL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), BAIAP2L1, IRTKS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), espF(U), tccP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 Escherichia coli O157:H7])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WASL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), BAIAP2L1, IRTKS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), espF(U), tccP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 Escherichia coli O157:H7])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [http://www.ebi.ac.uk/pdbsum/2lnh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [http://www.ebi.ac.uk/pdbsum/2lnh PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/WASL_HUMAN WASL_HUMAN]] Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.<ref>PMID:19366662</ref> [[http://www.uniprot.org/uniprot/ESFU3_ECO57 ESFU3_ECO57]] Required for efficient pedestal formation in host epithelial cells during infection. Acts as an intermediate between Tir (via host BAIAP2) and host WASL/N-WASP. Directly binds and activates WASL/N-WASP, which stimulates actin polymerization and leads to the formation of actin pedestals at the sites of bacterial adhesion.<ref>PMID:18650809</ref> <ref>PMID:22921828</ref> [[http://www.uniprot.org/uniprot/BI2L1_HUMAN BI2L1_HUMAN]] May function as adapter protein. Involved in the formation of clusters of actin bundles. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection.<ref>PMID:17430976</ref> <ref>PMID:19366662</ref> <ref>PMID:22921828</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	[[Category: Escherichia coli o157:h7]]		[[Category: Escherichia coli o157:h7]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Aitio, O.]]	+	[[Category: Aitio, O]]
-	[[Category: Hellman, M.]]	+	[[Category: Hellman, M]]
-	[[Category: Kesti, T.]]	+	[[Category: Kesti, T]]
-	[[Category: Leong, J M.]]	+	[[Category: Leong, J M]]
-	[[Category: Permi, P.]]	+	[[Category: Permi, P]]
-	[[Category: Saksela, K.]]	+	[[Category: Saksela, K]]
-	[[Category: Skehan, B.]]	+	[[Category: Skehan, B]]
	[[Category: Protein complex]]		[[Category: Protein complex]]
	[[Category: Signaling protein-protein binding complex]]		[[Category: Signaling protein-protein binding complex]]

OCA at 05:33, 29 September 2014

2014-09-29T05:33:05Z

←Older revision		Revision as of 05:33, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2lnh.~~png~~\|~~left\|200px~~]]	+	==Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly==
		+	<StructureSection load='2lnh' size='340' side='right' caption='[[2lnh]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LNH FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WASL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), BAIAP2L1, IRTKS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), espF(U), tccP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 Escherichia coli O157:H7])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [http://www.ebi.ac.uk/pdbsum/2lnh PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Intrinsically disordered protein (IDP)-mediated interactions are often characterized by low affinity but high specificity. These traits are essential in signaling and regulation that require reversibility. Enterohaemorrhagic Escherichia coli (EHEC) exploit this situation by commandeering host cytoskeletal signaling to stimulate actin assembly beneath bound bacteria, generating "pedestals" that promote intestinal colonization. EHEC translocates two proteins, EspF(U) and Tir, which form a complex with the host protein IRTKS. The interaction of this complex with N-WASP triggers localized actin polymerization. We show that EspF(U) is an IDP that contains a transiently alpha-helical N-terminus and dynamic C-terminus. Our structure shows that single EspF(U) repeat forms a high-affinity trimolecular complex with N-WASP and IRTKS. We demonstrate that bacterial and cellular ligands interact with IRTKS SH3 in a similar fashion, but the bacterial protein has evolved to outcompete cellular targets by utilizing a tryptophan switch that offers superior binding affinity enabling EHEC-induced pedestal formation.

-	~~{{STRUCTURE_2lnh\| PDB=2lnh \| SCENE= }}~~	+	Enterohaemorrhagic Escherichia Coli Exploits a Tryptophan Switch to Hijack Host F-Actin Assembly.,Aitio O, Hellman M, Skehan B, Kesti T, Leong JM, Saksela K, Permi P Structure. 2012 Aug 21. PMID:22921828<ref>PMID:22921828</ref>

-	~~===Enterohaemorrhagic E~~. ~~coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22921828}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[2lnh]] is a 3 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA].	+	</StructureSection>
	[[Category: Escherichia coli o157:h7]]		[[Category: Escherichia coli o157:h7]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 05:15, 12 September 2012

2012-09-12T05:15:30Z

←Older revision		Revision as of 05:15, 12 September 2012
Line 1:		Line 1:
-	[[Image:2lnh.~~jpg~~\|left\|200px]]	+	[[Image:2lnh.png\|left\|200px]]

	{{STRUCTURE_2lnh\| PDB=2lnh \| SCENE= }}		{{STRUCTURE_2lnh\| PDB=2lnh \| SCENE= }}
Line 5:		Line 5:
	===Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly===		===Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly===

		+	{{ABSTRACT_PUBMED_22921828}}

	==About this Structure==		==About this Structure==

OCA at 05:15, 29 August 2012

2012-08-29T05:15:46Z

←Older revision		Revision as of 05:15, 29 August 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:2lnh.jpg\|left\|200px]]

-	~~The entry~~ 2lnh ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_2lnh\| PDB=2lnh \| SCENE= }}

-	~~Authors: Aitio, O., Hellman, M., Skehan, B., Kesti, T., Leong, J.M., Saksela, K., Permi, P~~.	+	===Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly===

-	~~Description~~: ~~Enterohaemorrhagic E~~. coli ~~(EHEC) exploits a tryptophan switch to hijack host F~~-~~actin assembly~~	+
		+	==About this Structure==
		+	[[2lnh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA].
		+	[[Category: Escherichia coli o157:h7]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Aitio, O.]]
		+	[[Category: Hellman, M.]]
		+	[[Category: Kesti, T.]]
		+	[[Category: Leong, J M.]]
		+	[[Category: Permi, P.]]
		+	[[Category: Saksela, K.]]
		+	[[Category: Skehan, B.]]
		+	[[Category: Protein complex]]
		+	[[Category: Signaling protein-protein binding complex]]

OCA at 05:10, 8 March 2012

2012-03-08T05:10:46Z

←Older revision		Revision as of 05:10, 8 March 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 2lnh is ON HOLD	+	The entry 2lnh is ON HOLD until Paper Publication

	Authors: Aitio, O., Hellman, M., Skehan, B., Kesti, T., Leong, J.M., Saksela, K., Permi, P.		Authors: Aitio, O., Hellman, M., Skehan, B., Kesti, T., Leong, J.M., Saksela, K., Permi, P.

	Description: Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly		Description: Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly