2lvw - Revision history

OCA at 06:57, 1 May 2024

2024-05-01T06:57:46Z

←Older revision		Revision as of 06:57, 1 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2lvw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LVW FirstGlance]. <br>		<table><tr><td colspan='2'>[[2lvw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LVW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvw OCA], [https://pdbe.org/2lvw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lvw RCSB], [https://www.ebi.ac.uk/pdbsum/2lvw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lvw ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvw OCA], [https://pdbe.org/2lvw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lvw RCSB], [https://www.ebi.ac.uk/pdbsum/2lvw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lvw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/ILVN_ECOLI ILVN_ECOLI]		[https://www.uniprot.org/uniprot/ILVN_ECOLI ILVN_ECOLI]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The solution structure of IlvN, the regulatory subunit of Escherichia coli acetohydroxyacid synthase I, in the valine-bound form has been determined using high-resolution multidimensional, multinuclear nuclear magnetic resonance (NMR) methods. IlvN in the presence or absence of the effector molecule is present as a 22.5 kDa dimeric molecule. The ensemble of 20 low-energy structures shows a backbone root-mean-square deviation of 0.73 +/- 0.13 A and a root-mean-square deviation of 1.16 +/- 0.13 A for all heavy atoms. Furthermore, more than 98% of the backbone phi and psi dihedral angles occupy the allowed and additionally allowed regions of the Ramachandran map, which is indicative of the fact that the structures are of high stereochemical quality. Each protomer exhibits a betaalphabetabetaalphabetaalpha topology that is a characteristic feature of the ACT domain seen in metabolic enzymes. In the valine-bound form, IlvN exists apparently as a single conformer. In the free form, IlvN exists as a mixture of conformational states that are in intermediate exchange on the NMR time scale. Thus, a large shift in the conformational equilibrium is observed upon going from the free form to the bound form. The structure of the valine-bound form of IlvN was found to be similar to that of the ACT domain of the unliganded form of IlvH. Comparisons of the structures of the unliganded forms of these proteins suggest significant differences. The structural and conformational properties of IlvN determined here have allowed a better understanding of the mechanism of regulation of branched chain amino acid biosynthesis.
-
-	The coil-to-helix transition in IlvN regulates the allosteric control of Escherichia coli acetohydroxyacid synthase I.,Karanth NM, Sarma SP Biochemistry. 2013 Jan 8;52(1):70-83. doi: 10.1021/bi301415m. Epub 2012 Dec 21. PMID:23205845<ref>PMID:23205845</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2lvw" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:13, 22 February 2023

2023-02-22T13:13:31Z

←Older revision		Revision as of 13:13, 22 February 2023
Line 1:		Line 1:

	==Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)==		==Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)==
-	<StructureSection load='2lvw' size='340' side='right'caption='[[2lvw~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2lvw' size='340' side='right'caption='[[2lvw]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2lvw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LVW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2lvw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LVW FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3670, ilvN, JW3645 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvw OCA], [https://pdbe.org/2lvw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lvw RCSB], [https://www.ebi.ac.uk/pdbsum/2lvw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lvw ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvw OCA], [https://pdbe.org/2lvw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lvw RCSB], [https://www.ebi.ac.uk/pdbsum/2lvw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lvw ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ILVN_ECOLI ILVN_ECOLI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Acetolactate synthase]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Ecoli~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Karanth~~, M N~~]]	+	[[Category: Karanth MN]]
-	[[Category: Sarma~~, S P]]~~	+	[[Category: Sarma SP]]
-	~~[[Category: Act domain]]~~	+
-	~~[[Category: Aha]]~~	+
-	~~[[Category: Branched chain amino acid biosynthesis]]~~	+
-	~~[[Category: Ilvn]]~~	+
-	~~[[Category: Regulatory subunit]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Valine~~]]	+

OCA at 10:54, 19 May 2021

2021-05-19T10:54:42Z

←Older revision		Revision as of 10:54, 19 May 2021
Line 1:		Line 1:

	==Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)==		==Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)==
-	<StructureSection load='2lvw' size='340' side='right' caption='[[2lvw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2lvw' size='340' side='right'caption='[[2lvw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2lvw]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LVW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2LVW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2lvw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LVW FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3670, ilvN, JW3645 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3670, ilvN, JW3645 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2lvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvw OCA], [~~http~~://pdbe.org/2lvw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2lvw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2lvw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2lvw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvw OCA], [https://pdbe.org/2lvw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lvw RCSB], [https://www.ebi.ac.uk/pdbsum/2lvw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lvw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Karanth, M N]]		[[Category: Karanth, M N]]
	[[Category: Sarma, S P]]		[[Category: Sarma, S P]]

OCA at 23:11, 9 August 2018

2018-08-09T23:11:35Z

←Older revision		Revision as of 23:11, 9 August 2018
Line 1:		Line 1:
		+
	==Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)==		==Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)==
	<StructureSection load='2lvw' size='340' side='right' caption='[[2lvw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2lvw' size='340' side='right' caption='[[2lvw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3670, ilvN, JW3645 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3670, ilvN, JW3645 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvw OCA], [http://pdbe.org/2lvw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lvw RCSB], [http://www.ebi.ac.uk/pdbsum/2lvw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvw OCA], [http://pdbe.org/2lvw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lvw RCSB], [http://www.ebi.ac.uk/pdbsum/2lvw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lvw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:21, 11 September 2015

2015-09-11T01:21:31Z

←Older revision		Revision as of 01:21, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2lvw' size='340' side='right' caption='[[2lvw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2lvw' size='340' side='right' caption='[[2lvw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2lvw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LVW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LVW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2lvw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LVW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LVW FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3670, ilvN, JW3645 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ~~Escherichia coli K-12~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3670, ilvN, JW3645 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lvw RCSB], [http://www.ebi.ac.uk/pdbsum/2lvw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvw OCA], [http://pdbe.org/2lvw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lvw RCSB], [http://www.ebi.ac.uk/pdbsum/2lvw PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2lvw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 20:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
-	[[Category: ~~Escherichia coli k-12~~]]	+	[[Category: Ecoli]]
	[[Category: Karanth, M N]]		[[Category: Karanth, M N]]
	[[Category: Sarma, S P]]		[[Category: Sarma, S P]]

OCA at 12:47, 18 December 2014

2014-12-18T12:47:49Z

←Older revision		Revision as of 12:47, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2lvw\| PDB=2lvw \| SCENE= }}~~	+	==Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)==
-	===Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)===	+	<StructureSection load='2lvw' size='340' side='right' caption='[[2lvw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~{{ABSTRACT_PUBMED_23205845}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2lvw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LVW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LVW FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3670, ilvN, JW3645 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lvw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lvw RCSB], [http://www.ebi.ac.uk/pdbsum/2lvw PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The solution structure of IlvN, the regulatory subunit of Escherichia coli acetohydroxyacid synthase I, in the valine-bound form has been determined using high-resolution multidimensional, multinuclear nuclear magnetic resonance (NMR) methods. IlvN in the presence or absence of the effector molecule is present as a 22.5 kDa dimeric molecule. The ensemble of 20 low-energy structures shows a backbone root-mean-square deviation of 0.73 +/- 0.13 A and a root-mean-square deviation of 1.16 +/- 0.13 A for all heavy atoms. Furthermore, more than 98% of the backbone phi and psi dihedral angles occupy the allowed and additionally allowed regions of the Ramachandran map, which is indicative of the fact that the structures are of high stereochemical quality. Each protomer exhibits a betaalphabetabetaalphabetaalpha topology that is a characteristic feature of the ACT domain seen in metabolic enzymes. In the valine-bound form, IlvN exists apparently as a single conformer. In the free form, IlvN exists as a mixture of conformational states that are in intermediate exchange on the NMR time scale. Thus, a large shift in the conformational equilibrium is observed upon going from the free form to the bound form. The structure of the valine-bound form of IlvN was found to be similar to that of the ACT domain of the unliganded form of IlvH. Comparisons of the structures of the unliganded forms of these proteins suggest significant differences. The structural and conformational properties of IlvN determined here have allowed a better understanding of the mechanism of regulation of branched chain amino acid biosynthesis.

-	~~==About this Structure==~~	+	The coil-to-helix transition in IlvN regulates the allosteric control of Escherichia coli acetohydroxyacid synthase I.,Karanth NM, Sarma SP Biochemistry. 2013 Jan 8;52(1):70-83. doi: 10.1021/bi301415m. Epub 2012 Dec 21. PMID:23205845<ref>PMID:23205845</ref>
-	~~[[2lvw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k~~-12 Escherichia coli k-~~12]~~. ~~Full experimental information is available from [http~~:/~~/oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2LVW OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023205845</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Acetolactate synthase]]		[[Category: Acetolactate synthase]]
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
-	[[Category: Karanth, M N.]]	+	[[Category: Karanth, M N]]
-	[[Category: Sarma, S P.]]	+	[[Category: Sarma, S P]]
	[[Category: Act domain]]		[[Category: Act domain]]
	[[Category: Aha]]		[[Category: Aha]]

OCA at 16:07, 19 June 2013

2013-06-19T16:07:15Z

←Older revision		Revision as of 16:07, 19 June 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_2lvw\| PDB=2lvw \| SCENE= }}
		+	===Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)===
		+	{{ABSTRACT_PUBMED_23205845}}

-	~~The entry~~ 2lvw is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[2lvw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LVW OCA].

-	~~Authors~~: Karanth, M.N., Sarma, S.P.	+	==Reference==
-		+	<ref group="xtra">PMID:023205845</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Solution NMR studies of the dimeric regulatory~~ subunit ~~IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)~~	+	[[Category: Acetolactate synthase]]
		+	[[Category: Escherichia coli k-12]]
		+	[[Category: Karanth, M N.]]
		+	[[Category: Sarma, S P.]]
		+	[[Category: Act domain]]
		+	[[Category: Aha]]
		+	[[Category: Branched chain amino acid biosynthesis]]
		+	[[Category: Ilvn]]
		+	[[Category: Regulatory subunit]]
		+	[[Category: Transferase]]
		+	[[Category: Valine]]

OCA at 19:33, 17 April 2013

2013-04-17T19:33:34Z

←Older revision		Revision as of 19:33, 17 April 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 2lvw is ON HOLD	+	The entry 2lvw is ON HOLD until Paper Publication

	Authors: Karanth, M.N., Sarma, S.P.		Authors: Karanth, M.N., Sarma, S.P.

	Description: Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)		Description: Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)

OCA: Protected "2lvw" [edit=sysop:move=sysop]

2012-07-18T04:45:23Z

Protected "2lvw" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:45, 18 July 2012

OCA: New page: '''Unreleased structure''' The entry 2lvw is ON HOLD Authors: Karanth, M.N., Sarma, S.P. Description: Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohyd...

2012-07-18T04:45:22Z

New page: '''Unreleased structure''' The entry 2lvw is ON HOLD Authors: Karanth, M.N., Sarma, S.P. Description: Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohyd...

New page

'''Unreleased structure'''

The entry 2lvw is ON HOLD

Authors: Karanth, M.N., Sarma, S.P.

Description: Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)