2m9m - Revision history

OCA at 06:02, 15 May 2024

2024-05-15T06:02:46Z

←Older revision		Revision as of 06:02, 15 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2m9m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M9M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2m9m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M9M FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [https://pdbe.org/2m9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [https://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [https://pdbe.org/2m9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [https://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 09:31, 14 June 2023

2023-06-14T09:31:29Z

←Older revision		Revision as of 09:31, 14 June 2023
Line 3:		Line 3:
	<StructureSection load='2m9m' size='340' side='right'caption='[[2m9m]]' scene=''>		<StructureSection load='2m9m' size='340' side='right'caption='[[2m9m]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M9M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2m9m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M9M FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [https://pdbe.org/2m9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [https://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [https://pdbe.org/2m9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [https://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN] Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The FANCM/FAAP24 heterodimer has distinct functions in protecting cells from complex DNA lesions such as interstrand crosslinks. These functions rely on the biochemical activity of FANCM/FAAP24 to recognize and bind to damaged DNA or stalled replication forks. However, the DNA-binding activity of this complex was not clearly defined. We investigated how FAAP24 contributes to the DNA-interacting functions of the FANCM/FAAP24 complex by acquiring the N-terminal and C-terminal solution structures of human FAAP24. Modeling of the FAAP24 structure indicates that FAAP24 may possess a high affinity toward single-stranded DNA (ssDNA). Testing of various FAAP24 mutations in vitro and in vivo validated this prediction derived from structural analyses. We found that the DNA-binding and FANCM-interacting functions of FAAP24, although both require the C-terminal (HhH)2 domain, can be distinguished by segregation-of-function mutations. These results demonstrate dual roles of FAAP24 in DNA damage response against crosslinking lesions, one through the formation of FANCM/FAAP24 heterodimer and the other via its ssDNA-binding activity required in optimized checkpoint activation.Cell Research advance online publication 3 September 2013; doi:10.1038/cr.2013.124.
		+
		+	Structure analysis of FAAP24 reveals single-stranded DNA-binding activity and domain functions in DNA damage response.,Wang Y, Han X, Wu F, Leung JW, Lowery MG, Do H, Chen J, Shi C, Tian C, Li L, Gong W Cell Res. 2013 Sep 3. doi: 10.1038/cr.2013.124. PMID:23999858<ref>PMID:23999858</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 2m9m" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Gong W]]		[[Category: Gong W]]

OCA at 10:20, 31 August 2022

2022-08-31T10:20:59Z

←Older revision		Revision as of 10:20, 31 August 2022
Line 1:		Line 1:

	==Solution Structure of ERCC4 domain of human FAAP24==		==Solution Structure of ERCC4 domain of human FAAP24==
-	<StructureSection load='2m9m' size='340' side='right'caption='[[2m9m~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='2m9m' size='340' side='right'caption='[[2m9m]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[2m9m]] is a 1 chain structure.~~ Full ~~experimental~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M9M FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M9M FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2m9n\|2m9n]]</div></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [https://pdbe.org/2m9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [https://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [https://pdbe.org/2m9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [https://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr>	+
	</table>		</table>
-	== Function ==
-	[[https://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN]] Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The FANCM/FAAP24 heterodimer has distinct functions in protecting cells from complex DNA lesions such as interstrand crosslinks. These functions rely on the biochemical activity of FANCM/FAAP24 to recognize and bind to damaged DNA or stalled replication forks. However, the DNA-binding activity of this complex was not clearly defined. We investigated how FAAP24 contributes to the DNA-interacting functions of the FANCM/FAAP24 complex by acquiring the N-terminal and C-terminal solution structures of human FAAP24. Modeling of the FAAP24 structure indicates that FAAP24 may possess a high affinity toward single-stranded DNA (ssDNA). Testing of various FAAP24 mutations in vitro and in vivo validated this prediction derived from structural analyses. We found that the DNA-binding and FANCM-interacting functions of FAAP24, although both require the C-terminal (HhH)2 domain, can be distinguished by segregation-of-function mutations. These results demonstrate dual roles of FAAP24 in DNA damage response against crosslinking lesions, one through the formation of FANCM/FAAP24 heterodimer and the other via its ssDNA-binding activity required in optimized checkpoint activation.Cell Research advance online publication 3 September 2013; doi:10.1038/cr.2013.124.
-
-	Structure analysis of FAAP24 reveals single-stranded DNA-binding activity and domain functions in DNA damage response.,Wang Y, Han X, Wu F, Leung JW, Lowery MG, Do H, Chen J, Shi C, Tian C, Li L, Gong W Cell Res. 2013 Sep 3. doi: 10.1038/cr.2013.124. PMID:23999858<ref>PMID:23999858</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2m9m" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gong, W]]	+	[[Category: Gong W]]
-	[[Category: Han, X]]	+	[[Category: Han X]]
-	[[Category: Shi, C]]	+	[[Category: Shi C]]
-	[[Category: Tian, C]]	+	[[Category: Tian C]]
-	[[Category: Wu, F]]	+	[[Category: Wu F]]
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Ercc4 domain]]~~	+
-	~~[[Category: Faap24]]~~	+
-	~~[[Category: Fanconi anemia~~]]	+

OCA at 04:54, 25 August 2022

2022-08-25T04:54:31Z

←Older revision		Revision as of 04:54, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='2m9m' size='340' side='right'caption='[[2m9m]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='2m9m' size='340' side='right'caption='[[2m9m]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2m9m]] is a 1 chain structure ~~with sequence from [https://en.wikipedia.org/wiki/Human Human]~~. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M9M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2m9m]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M9M FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2m9n\|2m9n]]</div></td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2m9n\|2m9n]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FAAP24, C19orf40 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [https://pdbe.org/2m9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [https://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [https://pdbe.org/2m9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [https://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Gong, W]]		[[Category: Gong, W]]

OCA at 10:05, 26 May 2021

2021-05-26T10:05:36Z

←Older revision		Revision as of 10:05, 26 May 2021
Line 1:		Line 1:

	==Solution Structure of ERCC4 domain of human FAAP24==		==Solution Structure of ERCC4 domain of human FAAP24==
-	<StructureSection load='2m9m' size='340' side='right' caption='[[2m9m]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='2m9m' size='340' side='right'caption='[[2m9m]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2m9m]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2M9M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2m9m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M9M FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2m9n\|2m9n]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2m9n\|2m9n]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FAAP24, C19orf40 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FAAP24, C19orf40 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [~~http~~://pdbe.org/2m9m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [https://pdbe.org/2m9m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [https://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN]] Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.	+	[[https://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN]] Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Gong, W]]		[[Category: Gong, W]]
	[[Category: Han, X]]		[[Category: Han, X]]

OCA at 22:59, 9 August 2018

2018-08-09T22:59:00Z

←Older revision		Revision as of 22:59, 9 August 2018
Line 1:		Line 1:
		+
	==Solution Structure of ERCC4 domain of human FAAP24==		==Solution Structure of ERCC4 domain of human FAAP24==
	<StructureSection load='2m9m' size='340' side='right' caption='[[2m9m]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='2m9m' size='340' side='right' caption='[[2m9m]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2m9n\|2m9n]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2m9n\|2m9n]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FAAP24, C19orf40 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FAAP24, C19orf40 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [http://pdbe.org/2m9m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [http://www.ebi.ac.uk/pdbsum/2m9m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [http://pdbe.org/2m9m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [http://www.ebi.ac.uk/pdbsum/2m9m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2m9m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 20:18, 10 September 2015

2015-09-10T20:18:16Z

←Older revision		Revision as of 20:18, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2m9m' size='340' side='right' caption='[[2m9m]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='2m9m' size='340' side='right' caption='[[2m9m]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2m9m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M9M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2m9m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M9M FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2m9n\|2m9n]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2m9n\|2m9n]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FAAP24, C19orf40 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FAAP24, C19orf40 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [http://www.ebi.ac.uk/pdbsum/2m9m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [http://pdbe.org/2m9m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [http://www.ebi.ac.uk/pdbsum/2m9m PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2m9m" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Gong, W]]		[[Category: Gong, W]]
	[[Category: Han, X]]		[[Category: Han, X]]

OCA at 05:36, 25 December 2014

2014-12-25T05:36:25Z

←Older revision		Revision as of 05:36, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [http://www.ebi.ac.uk/pdbsum/2m9m PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [http://www.ebi.ac.uk/pdbsum/2m9m PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN]] Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:25, 18 December 2014

2014-12-18T12:25:41Z

←Older revision		Revision as of 12:25, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2m9m\| PDB=2m9m \| SCENE= }}~~	+	==Solution Structure of ERCC4 domain of human FAAP24==
-	===Solution Structure of ERCC4 domain of human FAAP24===	+	<StructureSection load='2m9m' size='340' side='right' caption='[[2m9m]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
-	~~{{ABSTRACT_PUBMED_23999858}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2m9m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M9M FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2m9n\|2m9n]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FAAP24, C19orf40 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m9m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m9m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m9m RCSB], [http://www.ebi.ac.uk/pdbsum/2m9m PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The FANCM/FAAP24 heterodimer has distinct functions in protecting cells from complex DNA lesions such as interstrand crosslinks. These functions rely on the biochemical activity of FANCM/FAAP24 to recognize and bind to damaged DNA or stalled replication forks. However, the DNA-binding activity of this complex was not clearly defined. We investigated how FAAP24 contributes to the DNA-interacting functions of the FANCM/FAAP24 complex by acquiring the N-terminal and C-terminal solution structures of human FAAP24. Modeling of the FAAP24 structure indicates that FAAP24 may possess a high affinity toward single-stranded DNA (ssDNA). Testing of various FAAP24 mutations in vitro and in vivo validated this prediction derived from structural analyses. We found that the DNA-binding and FANCM-interacting functions of FAAP24, although both require the C-terminal (HhH)2 domain, can be distinguished by segregation-of-function mutations. These results demonstrate dual roles of FAAP24 in DNA damage response against crosslinking lesions, one through the formation of FANCM/FAAP24 heterodimer and the other via its ssDNA-binding activity required in optimized checkpoint activation.Cell Research advance online publication 3 September 2013; doi:10.1038/cr.2013.124.

-	~~==Function==~~	+	Structure analysis of FAAP24 reveals single-stranded DNA-binding activity and domain functions in DNA damage response.,Wang Y, Han X, Wu F, Leung JW, Lowery MG, Do H, Chen J, Shi C, Tian C, Li L, Gong W Cell Res. 2013 Sep 3. doi: 10.1038/cr.2013.124. PMID:23999858<ref>PMID:23999858</ref>
-	~~[[http://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN]] Plays a role in DNA repair through recruitment~~ of ~~the FA core complex to damaged~~ DNA~~. Regulates FANCD2 monoubiquitination upon~~ DNA damage. ~~Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents~~, ~~when repressed~~. ~~Targets FANCM~~/~~FAAP24 complex to the DNA, preferentially to single strand DNA~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[2m9m]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:023999858</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Gong, W.]]	+	[[Category: Gong, W]]
-	[[Category: Han, X.]]	+	[[Category: Han, X]]
-	[[Category: Shi, C.]]	+	[[Category: Shi, C]]
-	[[Category: Tian, C.]]	+	[[Category: Tian, C]]
-	[[Category: Wu, F.]]	+	[[Category: Wu, F]]
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]
	[[Category: Ercc4 domain]]		[[Category: Ercc4 domain]]
	[[Category: Faap24]]		[[Category: Faap24]]
	[[Category: Fanconi anemia]]		[[Category: Fanconi anemia]]

OCA at 04:09, 20 September 2013

2013-09-20T04:09:59Z

←Older revision		Revision as of 04:09, 20 September 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_2m9m\| PDB=2m9m \| SCENE= }}
		+	===Solution Structure of ERCC4 domain of human FAAP24===
		+	{{ABSTRACT_PUBMED_23999858}}

-	~~The entry 2m9m is ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/FAP24_HUMAN FAP24_HUMAN]] Plays a role in DNA repair through recruitment of the FA core complex to damaged DNA. Regulates FANCD2 monoubiquitination upon DNA damage. Induces chromosomal instability as well as hypersensitivity to DNA cross-linking agents, when repressed. Targets FANCM/FAAP24 complex to the DNA, preferentially to single strand DNA.

-	~~Authors~~: ~~Wu, F~~.~~, Han, X~~.~~, Shi, C~~.~~, Gong, W~~.~~, Tian, C~~.	+	==About this Structure==
		+	[[2m9m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M9M OCA].

-	~~Description~~: ~~Solution Structure of ERCC4~~ domain ~~of human FAAP24~~	+	==Reference==
		+	<ref group="xtra">PMID:023999858</ref><references group="xtra"/><references/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Gong, W.]]
		+	[[Category: Han, X.]]
		+	[[Category: Shi, C.]]
		+	[[Category: Tian, C.]]
		+	[[Category: Wu, F.]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Ercc4 domain]]
		+	[[Category: Faap24]]
		+	[[Category: Fanconi anemia]]