2msj - Revision history

OCA at 10:16, 30 August 2023

2023-08-30T10:16:20Z

←Older revision		Revision as of 10:16, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='2msj' size='340' side='right'caption='[[2msj]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2msj' size='340' side='right'caption='[[2msj]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2msj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Blennius_americanus Blennius~~ americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MSJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2msj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MSJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2msj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2msj OCA], [https://pdbe.org/2msj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2msj RCSB], [https://www.ebi.ac.uk/pdbsum/2msj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2msj ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2msj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2msj OCA], [https://pdbe.org/2msj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2msj RCSB], [https://www.ebi.ac.uk/pdbsum/2msj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2msj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ANP12_ZOAAM ANP12_ZOAAM] Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Blennius americanus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baardsnes, J]]	+	[[Category: Zoarces americanus]]
-	[[Category: Davies~~, P L~~]]	+	[[Category: Baardsnes J]]
-	[[Category: Deluca~~, C I~~]]	+	[[Category: Davies PL]]
-	[[Category: Graether~~, S P~~]]	+	[[Category: Deluca CI]]
-	[[Category: Hill~~, G A~~]]	+	[[Category: Graether SP]]
-	[[Category: Jia, Z]]	+	[[Category: Hill GA]]
-	~~[[Category: Antifreeze protein]]~~	+	[[Category: Jia Z]]
-	~~[[Category: Ice binding protein]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: Thermal hysteresis protein~~]]	+

OCA at 15:43, 3 November 2021

2021-11-03T15:43:56Z

←Older revision		Revision as of 15:43, 3 November 2021
Line 3:		Line 3:
	<StructureSection load='2msj' size='340' side='right'caption='[[2msj]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2msj' size='340' side='right'caption='[[2msj]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2msj]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2MSJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2msj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MSJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2msj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2msj OCA], [~~http~~://pdbe.org/2msj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2msj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2msj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2msj ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2msj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2msj OCA], [https://pdbe.org/2msj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2msj RCSB], [https://www.ebi.ac.uk/pdbsum/2msj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2msj ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 06:27, 20 May 2020

2020-05-20T06:27:45Z

←Older revision		Revision as of 06:27, 20 May 2020
Line 1:		Line 1:

	==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 N46S==		==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 N46S==
-	<StructureSection load='2msj' size='340' side='right' caption='[[2msj]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='2msj' size='340' side='right'caption='[[2msj]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2msj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2MSJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2msj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2MSJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2msj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2msj OCA], [http://pdbe.org/2msj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2msj RCSB], [http://www.ebi.ac.uk/pdbsum/2msj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2msj ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2msj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2msj OCA], [http://pdbe.org/2msj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2msj RCSB], [http://www.ebi.ac.uk/pdbsum/2msj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2msj ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 27:		Line 27:

	==See Also==		==See Also==
-	*[[Antifreeze protein\|Antifreeze protein]]	+	*[[Antifreeze protein 3D structures\|Antifreeze protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Blennius americanus]]		[[Category: Blennius americanus]]
		+	[[Category: Large Structures]]
	[[Category: Baardsnes, J]]		[[Category: Baardsnes, J]]
	[[Category: Davies, P L]]		[[Category: Davies, P L]]

OCA at 16:50, 15 August 2018

2018-08-15T16:50:30Z

←Older revision		Revision as of 16:50, 15 August 2018
Line 1:		Line 1:
		+
	==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 N46S==		==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 N46S==
	<StructureSection load='2msj' size='340' side='right' caption='[[2msj]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2msj' size='340' side='right' caption='[[2msj]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2msj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MSJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2msj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MSJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2msj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2msj OCA], [http://pdbe.org/2msj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2msj RCSB], [http://www.ebi.ac.uk/pdbsum/2msj PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2msj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2msj OCA], [http://pdbe.org/2msj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2msj RCSB], [http://www.ebi.ac.uk/pdbsum/2msj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2msj ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 9:		Line 10:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/2msj_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/2msj_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2msj ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:50, 11 September 2015

2015-09-11T11:50:59Z

←Older revision		Revision as of 11:50, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2msj' size='340' side='right' caption='[[2msj]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2msj' size='340' side='right' caption='[[2msj]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2msj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Zoarces_americanus Zoarces~~ americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MSJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2msj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_americanus Blennius americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MSJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2msj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2msj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2msj RCSB], [http://www.ebi.ac.uk/pdbsum/2msj PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2msj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2msj OCA], [http://pdbe.org/2msj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2msj RCSB], [http://www.ebi.ac.uk/pdbsum/2msj PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 23:		Line 23:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2msj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 30:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Zoarces~~ americanus]]	+	[[Category: Blennius americanus]]
	[[Category: Baardsnes, J]]		[[Category: Baardsnes, J]]
	[[Category: Davies, P L]]		[[Category: Davies, P L]]

OCA at 16:04, 25 December 2014

2014-12-25T16:04:57Z

←Older revision		Revision as of 16:04, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2msj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MSJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2msj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MSJ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2msj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2msj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2msj RCSB], [http://www.ebi.ac.uk/pdbsum/2msj PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2msj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2msj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2msj RCSB], [http://www.ebi.ac.uk/pdbsum/2msj PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Zoarces americanus]]		[[Category: Zoarces americanus]]
-	[[Category: Baardsnes, J.]]	+	[[Category: Baardsnes, J]]
-	[[Category: Davies, P L.]]	+	[[Category: Davies, P L]]
-	[[Category: Deluca, C I.]]	+	[[Category: Deluca, C I]]
-	[[Category: Graether, S P.]]	+	[[Category: Graether, S P]]
-	[[Category: Hill, G A.]]	+	[[Category: Hill, G A]]
-	[[Category: Jia, Z.]]	+	[[Category: Jia, Z]]
	[[Category: Antifreeze protein]]		[[Category: Antifreeze protein]]
	[[Category: Ice binding protein]]		[[Category: Ice binding protein]]
	[[Category: Mutant]]		[[Category: Mutant]]
	[[Category: Thermal hysteresis protein]]		[[Category: Thermal hysteresis protein]]

OCA at 03:20, 29 September 2014

2014-09-29T03:20:06Z

←Older revision		Revision as of 03:20, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2msj.~~png\|left~~\|200px]]	+	==TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 N46S==
		+	<StructureSection load='2msj' size='340' side='right' caption='[[2msj]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2msj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MSJ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2msj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2msj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2msj RCSB], [http://www.ebi.ac.uk/pdbsum/2msj PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/2msj_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Some cold water marine fishes avoid cellular damage because of freezing by expressing antifreeze proteins (AFPs) that bind to ice and inhibit its growth; one such protein is the globular type III AFP from eel pout. Despite several studies, the mechanism of ice binding remains unclear because of the difficulty in modeling the AFP-ice interaction. To further explore the mechanism, we have determined the x-ray crystallographic structure of 10 type III AFP mutants and combined that information with 7 previously determined structures to mainly analyze specific AFP-ice interactions such as hydrogen bonds. Quantitative assessment of binding was performed using a neural network with properties of the structure as input and predicted antifreeze activity as output. Using the cross-validation method, a correlation coefficient of 0.60 was obtained between measured and predicted activity, indicating successful learning and good predictive power. A large loss in the predictive power of the neural network occurred after properties related to the hydrophobic surface were left out, suggesting that van der Waal's interactions make a significant contribution to ice binding. By combining the analysis of the neural network with antifreeze activity and x-ray crystallographic structures of the mutants, we extend the existing ice-binding model to a two-step process: 1) probing of the surface for the correct ice-binding plane by hydrogen-bonding side chains and 2) attractive van der Waal's interactions between the other residues of the ice-binding surface and the ice, which increases the strength of the protein-ice interaction.

-	~~{{STRUCTURE_2msj\| PDB=2msj \| SCENE= }}~~	+	Quantitative and qualitative analysis of type III antifreeze protein structure and function.,Graether SP, DeLuca CI, Baardsnes J, Hill GA, Davies PL, Jia Z J Biol Chem. 1999 Apr 23;274(17):11842-7. PMID:10207002<ref>PMID:10207002</ref>

-	~~===TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 N46S===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10207002}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2msj]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Zoarces_americanus Zoarces americanus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA].~~	+

	==See Also==		==See Also==
	*[[Antifreeze protein\|Antifreeze protein]]		*[[Antifreeze protein\|Antifreeze protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010207002<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Zoarces americanus]]		[[Category: Zoarces americanus]]
	[[Category: Baardsnes, J.]]		[[Category: Baardsnes, J.]]

OCA at 18:41, 20 October 2012

2012-10-20T18:41:44Z

←Older revision		Revision as of 18:41, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[2msj]] is a 1 chain structure ~~of [[Antifreeze protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA].	+	[[2msj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA].

	==See Also==		==See Also==

OCA: Protected "2msj" [edit=sysop:move=sysop]

2012-07-27T04:55:32Z

Protected "2msj" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:55, 27 July 2012

OCA at 04:55, 27 July 2012

2012-07-27T04:55:30Z

←Older revision		Revision as of 04:55, 27 July 2012
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-	{{Seed}}
	[[Image:2msj.png\|left\|200px]]		[[Image:2msj.png\|left\|200px]]

-	<!--
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-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_2msj\| PDB=2msj \| SCENE= }}		{{STRUCTURE_2msj\| PDB=2msj \| SCENE= }}

	===TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 N46S===		===TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 N46S===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10207002}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_10207002}}		{{ABSTRACT_PUBMED_10207002}}

	==About this Structure==		==About this Structure==
-	~~2MSJ~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/~~Macrozoarces_americanus Macrozoarces~~ americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA].	+	[[2msj]] is a 1 chain structure of [[Antifreeze protein]] with sequence from [http://en.wikipedia.org/wiki/Zoarces_americanus Zoarces americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MSJ OCA].
		+
		+	==See Also==
		+	*[[Antifreeze protein\|Antifreeze protein]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10207002~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010207002</ref><references group="xtra"/>
-	[[Category: ~~Macrozoarces~~ americanus]]	+	[[Category: Zoarces americanus]]
	[[Category: Baardsnes, J.]]		[[Category: Baardsnes, J.]]
	[[Category: Davies, P L.]]		[[Category: Davies, P L.]]
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	[[Category: Mutant]]		[[Category: Mutant]]
	[[Category: Thermal hysteresis protein]]		[[Category: Thermal hysteresis protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:15:43 2009''