2n70 - Revision history

OCA at 10:22, 15 March 2023

2023-03-15T10:22:08Z

←Older revision		Revision as of 10:22, 15 March 2023
Line 1:		Line 1:

	==Two-fold symmetric structure of the 18-60 construct of S31N M2 from Influenza A in lipid bilayers==		==Two-fold symmetric structure of the 18-60 construct of S31N M2 from Influenza A in lipid bilayers==
-	<StructureSection load='2n70' size='340' side='right'caption='[[2n70~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2n70' size='340' side='right'caption='[[2n70]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n70]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N70 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n70]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Udorn/307/1972(H3N2)) Influenza A virus (A/Udorn/307/1972(H3N2))]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N70 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n70 OCA], [https://pdbe.org/2n70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n70 RCSB], [https://www.ebi.ac.uk/pdbsum/2n70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n70 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n70 OCA], [https://pdbe.org/2n70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n70 RCSB], [https://www.ebi.ac.uk/pdbsum/2n70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n70 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/M2_I72A2 M2_I72A2]] Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation (By similarity).<ref>PMID:7508997</ref>	+	[https://www.uniprot.org/uniprot/M2_I72A2 M2_I72A2] Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation (By similarity).<ref>PMID:7508997</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Andreas~~, L B~~]]	+	[[Category: Andreas LB]]
-	[[Category: Chou~~, J J~~]]	+	[[Category: Chou JJ]]
-	[[Category: Eddy~~, M T~~]]	+	[[Category: Eddy MT]]
-	[[Category: Emsley, L]]	+	[[Category: Emsley L]]
-	[[Category: Gelev, V]]	+	[[Category: Gelev V]]
-	[[Category: Griffin~~, R G~~]]	+	[[Category: Griffin RG]]
-	[[Category: Miller~~, E A~~]]	+	[[Category: Miller EA]]
-	[[Category: Ni, Q]]	+	[[Category: Ni Q]]
-	[[Category: Pintacuda, G]]	+	[[Category: Pintacuda G]]
-	[[Category: Reese, M]]	+	[[Category: Reese M]]
-	~~[[Category: Influenza]]~~	+
-	~~[[Category: M2]]~~	+
-	~~[[Category: S31n]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 15:41, 8 June 2021

2021-06-08T15:41:04Z

←Older revision		Revision as of 15:41, 8 June 2021
Line 3:		Line 3:
	<StructureSection load='2n70' size='340' side='right'caption='[[2n70]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2n70' size='340' side='right'caption='[[2n70]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n70]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N70 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2N70 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n70]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N70 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2n70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n70 OCA], [~~http~~://pdbe.org/2n70 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2n70 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2n70 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2n70 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n70 OCA], [https://pdbe.org/2n70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n70 RCSB], [https://www.ebi.ac.uk/pdbsum/2n70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n70 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/M2_I72A2 M2_I72A2]] Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation (By similarity).<ref>PMID:7508997</ref>	+	[[https://www.uniprot.org/uniprot/M2_I72A2 M2_I72A2]] Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation (By similarity).<ref>PMID:7508997</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:

	==See Also==		==See Also==
-	*[[Ion channels\|Ion channels]]	+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:34, 21 August 2019

2019-08-21T07:34:21Z

←Older revision		Revision as of 07:34, 21 August 2019
Line 1:		Line 1:

	==Two-fold symmetric structure of the 18-60 construct of S31N M2 from Influenza A in lipid bilayers==		==Two-fold symmetric structure of the 18-60 construct of S31N M2 from Influenza A in lipid bilayers==
-	<StructureSection load='2n70' size='340' side='right' caption='[[2n70]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2n70' size='340' side='right'caption='[[2n70]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2n70]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N70 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2n70]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N70 FirstGlance]. <br>
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 2n70" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2n70" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ion channels\|Ion channels]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Andreas, L B]]		[[Category: Andreas, L B]]
	[[Category: Chou, J J]]		[[Category: Chou, J J]]

OCA at 06:27, 26 July 2016

2016-07-26T06:27:21Z

←Older revision		Revision as of 06:27, 26 July 2016
Line 1:		Line 1:
		+
	==Two-fold symmetric structure of the 18-60 construct of S31N M2 from Influenza A in lipid bilayers==		==Two-fold symmetric structure of the 18-60 construct of S31N M2 from Influenza A in lipid bilayers==
	<StructureSection load='2n70' size='340' side='right' caption='[[2n70]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2n70' size='340' side='right' caption='[[2n70]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2n70]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N70 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2n70]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N70 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n70 OCA], [http://pdbe.org/2n70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n70 RCSB], [http://www.ebi.ac.uk/pdbsum/2n70 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n70 OCA], [http://pdbe.org/2n70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n70 RCSB], [http://www.ebi.ac.uk/pdbsum/2n70 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n70 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 07:40, 16 December 2015

2015-12-16T07:40:45Z

←Older revision		Revision as of 07:40, 16 December 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Two-fold symmetric structure of the 18-60 construct of S31N M2 from Influenza A in lipid bilayers==
		+	<StructureSection load='2n70' size='340' side='right' caption='[[2n70]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2n70]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N70 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n70 OCA], [http://pdbe.org/2n70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n70 RCSB], [http://www.ebi.ac.uk/pdbsum/2n70 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/M2_I72A2 M2_I72A2]] Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation (By similarity).<ref>PMID:7508997</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We report a magic angle spinning (MAS) NMR structure of the drug-resistant S31N mutation of M218-60 from Influenza A. The protein was dispersed in diphytanoyl-sn-glycero-3-phosphocholine lipid bilayers, and the spectra and an extensive set of constraints indicate that M218-60 consists of a dimer of dimers. In particular, approximately 280 structural constraints were obtained using dipole recoupling experiments that yielded well-resolved (13)C-(15)N, (13)C-(13)C, and (1)H-(15)N 2D, 3D, and 4D MAS spectra, all of which show cross-peak doubling. Interhelical distances were measured using mixed (15)N/(13)C labeling and with deuterated protein, MAS at omegar/2pi = 60 kHz, omega0H/2pi = 1000 MHz, and (1)H detection of methyl-methyl contacts. The experiments reveal a compact structure consisting of a tetramer composed of four transmembrane helices, in which two opposing helices are displaced and rotated in the direction of the membrane normal relative to a four-fold symmetric arrangement, yielding a two-fold symmetric structure. Side chain conformations of the important gating and pH-sensing residues W41 and H37 are found to differ markedly from four-fold symmetry. The rmsd of the structure is 0.7 A for backbone heavy atoms and 1.1 A for all heavy atoms. This two-fold symmetric structure is different from all of the previous structures of M2, many of which were determined in detergent and/or with shorter constructs that are not fully active. The structure has implications for the mechanism of H(+) transport since the distance between His and Trp residues on different helices is found to be short. The structure also exhibits two-fold symmetry in the vicinity of the binding site of adamantyl inhibitors, and steric constraints may explain the mechanism of the drug-resistant S31N mutation.

-	~~The entry 2n70 is ON HOLD~~	+	Structure and Mechanism of the Influenza A M218-60 Dimer of Dimers.,Andreas LB, Reese M, Eddy MT, Gelev V, Ni QZ, Miller EA, Emsley L, Pintacuda G, Chou JJ, Griffin RG J Am Chem Soc. 2015 Dec 2;137(47):14877-86. doi: 10.1021/jacs.5b04802. Epub 2015 , Aug 31. PMID:26218479<ref>PMID:26218479</ref>

-	~~Authors~~: Andreas, L.B~~., Reese, M.~~, Eddy, M.T~~., Gelev, V., Ni, Q., Miller, E.A.,~~ Emsley, L~~., Pintacuda, G., Chou, J.J.~~, Griffin, R.G.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Two-fold symmetric structure of the 18-60 construct of S31N M2 from Influenza A in lipid bilayers~~	+	<div class="pdbe-citations 2n70" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Andreas, L B]]
		+	[[Category: Chou, J J]]
		+	[[Category: Eddy, M T]]
		+	[[Category: Emsley, L]]
		+	[[Category: Gelev, V]]
		+	[[Category: Griffin, R G]]
		+	[[Category: Miller, E A]]
	[[Category: Ni, Q]]		[[Category: Ni, Q]]
-	[[Category: ~~Emsley~~, ~~L]]~~	+	[[Category: Pintacuda, G]]
-	~~[[Category: Eddy, M.T~~]]	+
	[[Category: Reese, M]]		[[Category: Reese, M]]
-	[[Category: ~~Griffin, R.G~~]]	+	[[Category: Influenza]]
-	[[Category: ~~Andreas, L.B~~]]	+	[[Category: M2]]
-	[[Category: ~~Chou, J.J~~]]	+	[[Category: S31n]]
-	[[Category: ~~Pintacuda, G]]~~	+	[[Category: Viral protein]]
-	~~[[Category: Miller, E.A]]~~	+
-	~~[[Category: Gelev, V~~]]	+

OCA: Protected "2n70" [edit=sysop:move=sysop]

2015-09-09T05:25:41Z

Protected "2n70" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:25, 9 September 2015

OCA: New page: '''Unreleased structure''' The entry 2n70 is ON HOLD Authors: Andreas, L.B., Reese, M., Eddy, M.T., Gelev, V., Ni, Q., Miller, E.A., Emsley, L., Pintacuda, G., Chou, J.J., Griffin, R.G....

2015-09-09T05:25:40Z

New page: '''Unreleased structure''' The entry 2n70 is ON HOLD Authors: Andreas, L.B., Reese, M., Eddy, M.T., Gelev, V., Ni, Q., Miller, E.A., Emsley, L., Pintacuda, G., Chou, J.J., Griffin, R.G....

New page

'''Unreleased structure'''

The entry 2n70 is ON HOLD

Authors: Andreas, L.B., Reese, M., Eddy, M.T., Gelev, V., Ni, Q., Miller, E.A., Emsley, L., Pintacuda, G., Chou, J.J., Griffin, R.G.

Description: Two-fold symmetric structure of the 18-60 construct of S31N M2 from Influenza A in lipid bilayers
[[Category: Unreleased Structures]]
[[Category: Ni, Q]]
[[Category: Emsley, L]]
[[Category: Eddy, M.T]]
[[Category: Reese, M]]
[[Category: Griffin, R.G]]
[[Category: Andreas, L.B]]
[[Category: Chou, J.J]]
[[Category: Pintacuda, G]]
[[Category: Miller, E.A]]
[[Category: Gelev, V]]