2naw - Revision history

OCA at 09:59, 14 June 2023

2023-06-14T09:59:46Z

←Older revision		Revision as of 09:59, 14 June 2023
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	==NMR solution structure of Exendin-4/conotoxin chimera (Ex-4[1-27]/pl14a)==		==NMR solution structure of Exendin-4/conotoxin chimera (Ex-4[1-27]/pl14a)==
-	<StructureSection load='2naw' size='340' side='right'caption='[[2naw~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2naw' size='340' side='right'caption='[[2naw]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2naw]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NAW FirstGlance]. <br>		<table><tr><td colspan='2'>[[2naw]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NAW FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2nav\|2nav]]</div></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2naw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2naw OCA], [https://pdbe.org/2naw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2naw RCSB], [https://www.ebi.ac.uk/pdbsum/2naw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2naw ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2naw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2naw OCA], [https://pdbe.org/2naw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2naw RCSB], [https://www.ebi.ac.uk/pdbsum/2naw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2naw ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CJEA_CONPO CJEA_CONPO] [https://www.uniprot.org/uniprot/EXE4_HELSU EXE4_HELSU] Venom protein that mimics the incretin hormone glucagon-like peptide 1 (GLP-1). It stimulates insulin synthesis and secretion, protects against beta-cell apoptosis in response to different insults, and promotes beta-cell proliferation. It also promotes satiety, reduces food intake, reduces fat deposition, reduces body weight and inhibits gastric emptying. Interacts with GLP-1 receptor (GLP1R). Induces hypotension that is mediated by relaxation of cardiac smooth muscle.<ref>PMID:8405712</ref> <ref>PMID:19837656</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Craik~~, D J~~]]	+	[[Category: Craik DJ]]
-	[[Category: Schroeder~~, C I~~]]	+	[[Category: Schroeder CI]]
-	[[Category: Swedberg~~, J E]]~~	+	[[Category: Swedberg JE]]
-	~~[[Category: Agonist]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 09:06, 23 February 2022

2022-02-23T09:06:10Z

←Older revision		Revision as of 09:06, 23 February 2022
Line 3:		Line 3:
	<StructureSection load='2naw' size='340' side='right'caption='[[2naw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2naw' size='340' side='right'caption='[[2naw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2naw]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NAW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2NAW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2naw]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NAW FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2nav\|2nav]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2nav\|2nav]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2naw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2naw OCA], [~~http~~://pdbe.org/2naw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2naw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2naw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2naw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2naw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2naw OCA], [https://pdbe.org/2naw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2naw RCSB], [https://www.ebi.ac.uk/pdbsum/2naw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2naw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:32, 20 May 2020

2020-05-20T06:32:45Z

←Older revision		Revision as of 06:32, 20 May 2020
Line 1:		Line 1:

	==NMR solution structure of Exendin-4/conotoxin chimera (Ex-4[1-27]/pl14a)==		==NMR solution structure of Exendin-4/conotoxin chimera (Ex-4[1-27]/pl14a)==
-	<StructureSection load='2naw' size='340' side='right' caption='[[2naw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2naw' size='340' side='right'caption='[[2naw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2naw]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NAW OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2NAW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2naw]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NAW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2NAW FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2nav\|2nav]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2nav\|2nav]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2naw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2naw OCA], [http://pdbe.org/2naw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2naw RCSB], [http://www.ebi.ac.uk/pdbsum/2naw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2naw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2naw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2naw OCA], [http://pdbe.org/2naw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2naw RCSB], [http://www.ebi.ac.uk/pdbsum/2naw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2naw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Craik, D J]]		[[Category: Craik, D J]]
	[[Category: Schroeder, C I]]		[[Category: Schroeder, C I]]

OCA at 05:09, 9 September 2016

2016-09-09T05:09:09Z

←Older revision		Revision as of 05:09, 9 September 2016
Line 5:		Line 5:
	<table><tr><td colspan='2'>[[2naw]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NAW FirstGlance]. <br>		<table><tr><td colspan='2'>[[2naw]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NAW FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2nav\|2nav]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2nav\|2nav]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2naw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2naw OCA], [http://pdbe.org/2naw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2naw RCSB], [http://www.ebi.ac.uk/pdbsum/2naw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2naw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2naw OCA], [http://pdbe.org/2naw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2naw RCSB], [http://www.ebi.ac.uk/pdbsum/2naw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2naw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:30, 20 June 2016

2016-06-20T07:30:27Z

←Older revision		Revision as of 07:30, 20 June 2016
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2naw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2naw OCA], [http://pdbe.org/2naw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2naw RCSB], [http://www.ebi.ac.uk/pdbsum/2naw PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2naw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2naw OCA], [http://pdbe.org/2naw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2naw RCSB], [http://www.ebi.ac.uk/pdbsum/2naw PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glucagon-like peptide-1 (GLP-1) signaling through the glucagon-like peptide 1 receptor (GLP-1R) is a key regulator of normal glucose metabolism, and exogenous GLP-1R agonist therapy is a promising avenue for the treatment of type 2 diabetes mellitus. To date, the development of therapeutic GLP-1R agonists has focused on producing drugs with extended serum half-life. This has been achieved by engineering synthetic analogs of GLP-1 or the more stable exogenous GLP-1R agonist exendin-4 (Ex-4). These synthetic peptide hormones share the overall structure of GLP-1 and Ex-4, with a C-terminal helical segment and a flexible N-terminal tail. Although numerous studies have investigated the molecular determinants underpinning GLP-1 and Ex-4 binding and signaling through the GLP-1R, these have primarily focused on the length and composition of the N-terminal tail or on how to modulate the helicity of the full-length peptides. Here, we investigate the effect of C-terminal truncation in GLP-1 and Ex-4 on the cAMP pathway. To ensure helical C-terminal regions in the truncated peptides, we produced a series of chimeric peptides combining the N-terminal portion of GLP-1 or Ex-4 and the C-terminal segment of the helix-promoting peptide alpha-conotoxin pl14a. The helicity and structures of the chimeric peptides were confirmed using circular dichroism and NMR, respectively. We found no direct correlation between the fraction helicity and potency in signaling via the cAMP pathway. Rather, the most important feature for efficient receptor binding and signaling was the C-terminal helical segment (residues 22-27) directing the binding of Phe22 into a hydrophobic pocket on the GLP-1R.
		+
		+	Truncated glucagon-like peptide-1 and exendin-4 alpha-conotoxin pl14a peptide chimeras maintain potency, alpha-helicity and reveal interactions vital for cAMP signaling in vitro.,Swedberg JE, Schroeder CI, Mitchell J, Fairlie DP, Edmonds DJ, Griffith DA, Ruggeri RB, Derksen DR, Loria PM, Price DA, Liras S, Craik DJ J Biol Chem. 2016 May 10. pii: jbc.M116.724542. PMID:27226591<ref>PMID:27226591</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 2naw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:07, 1 June 2016

2016-06-01T08:07:13Z

←Older revision		Revision as of 08:07, 1 June 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 2naw ~~is ON HOLD~~	+	==NMR solution structure of Exendin-4/conotoxin chimera (Ex-4[1-27]/pl14a)==
-		+	<StructureSection load='2naw' size='340' side='right' caption='[[2naw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~Authors~~: ~~Schroeder, C~~.I.~~, Swedberg, J~~.E.~~, Craik, D~~.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[2naw]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NAW FirstGlance]. <br>
-	~~Description~~: Ex-4[1-27]/~~pl14a~~	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2nav\|2nav]]</td></tr>
-	[[~~Category~~: ~~Unreleased Structures]~~]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2naw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2naw OCA], [http://pdbe.org/2naw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2naw RCSB], [http://www.ebi.ac.uk/pdbsum/2naw PDBsum]</span></td></tr>
-	[[Category: ~~Swedberg~~, J.E]]	+	</table>
-	[[Category: Schroeder, C.I]]	+	__TOC__
-	[[Category: ~~Craik~~, D.J]]	+	</StructureSection>
		+	[[Category: Craik, D J]]
		+	[[Category: Schroeder, C I]]
		+	[[Category: Swedberg, J E]]
		+	[[Category: Agonist]]
		+	[[Category: Toxin]]

OCA: Protected "2naw" [edit=sysop:move=sysop]

2016-01-20T09:24:49Z

Protected "2naw" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:24, 20 January 2016

OCA: New page: '''Unreleased structure''' The entry 2naw is ON HOLD Authors: Schroeder, C.I., Swedberg, J.E., Craik, D.J. Description: Ex-4[1-27]/pl14a Category: Unreleased Structures [[Category:...

2016-01-20T09:24:47Z

New page: '''Unreleased structure''' The entry 2naw is ON HOLD Authors: Schroeder, C.I., Swedberg, J.E., Craik, D.J. Description: Ex-4[1-27]/pl14a Category: Unreleased Structures [[Category:...

New page

'''Unreleased structure'''

The entry 2naw is ON HOLD

Authors: Schroeder, C.I., Swedberg, J.E., Craik, D.J.

Description: Ex-4[1-27]/pl14a
[[Category: Unreleased Structures]]
[[Category: Swedberg, J.E]]
[[Category: Schroeder, C.I]]
[[Category: Craik, D.J]]