2ntq - Revision history

OCA at 10:20, 30 August 2023

2023-08-30T10:20:34Z

←Older revision		Revision as of 10:20, 30 August 2023
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	<StructureSection load='2ntq' size='340' side='right'caption='[[2ntq]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2ntq' size='340' side='right'caption='[[2ntq]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ntq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Dicd3 Dicd3~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NTQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ntq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_dadantii_3937 Dickeya dadantii 3937]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NTQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADA:ALPHA~~-~~D-GALACTOPYRANURONIC+ACID'>ADA</scene>~~, ~~<scene name='pdbligand=M8C:METHYL+ALPHA-D-GALACTOPYRANURONATE'>M8C</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[1qjv\|1qjv]], [[2nsp\|2nsp]], [[2nst\|2nst]], [[2nt6\|2nt6]], [[2nt9\|2nt9]], [[2ntb\|2ntb]], [[2ntp\|2ntp]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=M8C:METHYL+ALPHA-D-GALACTOPYRANURONATE'>M8C</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>pemA, pem ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~198628 DICD3])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.11 3.1.1.11]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ntq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntq OCA], [https://pdbe.org/2ntq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ntq RCSB], [https://www.ebi.ac.uk/pdbsum/2ntq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ntq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ntq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntq OCA], [https://pdbe.org/2ntq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ntq RCSB], [https://www.ebi.ac.uk/pdbsum/2ntq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ntq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PMEA_DICD3 PMEA_DICD3]] Involved in maceration and soft-rotting of plant tissue.	+	[https://www.uniprot.org/uniprot/PMEA_DICD3 PMEA_DICD3] Involved in maceration and soft-rotting of plant tissue.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dicd3]]~~	+	[[Category: Dickeya dadantii 3937]]
-	~~[[Category: Hydrolase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brocklehurst, K]]	+	[[Category: Brocklehurst K]]
-	[[Category: Fries, M]]	+	[[Category: Fries M]]
-	[[Category: Pickersgill~~, R W~~]]	+	[[Category: Pickersgill RW]]
-	[[Category: Shevchik~~, V E]]~~	+	[[Category: Shevchik VE]]
-	~~[[Category: Pme hexasaccharide complex~~]]	+

OCA at 11:37, 16 February 2022

2022-02-16T11:37:36Z

OCA at 05:40, 13 May 2020

2020-05-13T05:40:01Z

←Older revision		Revision as of 05:40, 13 May 2020
Line 1:		Line 1:

	==Crystal structure of pectin methylesterase in complex with hexasaccharide VII==		==Crystal structure of pectin methylesterase in complex with hexasaccharide VII==
-	<StructureSection load='2ntq' size='340' side='right' caption='[[2ntq]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='2ntq' size='340' side='right'caption='[[2ntq]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ntq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2NTQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ntq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2NTQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=M8C:METHYL+ALPHA-D-GALACTOPYRANURONATE'>M8C</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=M8C:METHYL+ALPHA-D-GALACTOPYRANURONATE'>M8C</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qjv\|1qjv]], [[2nsp\|2nsp]], [[2nst\|2nst]], [[2nt6\|2nt6]], [[2nt9\|2nt9]], [[2ntb\|2ntb]], [[2ntp\|2ntp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qjv\|1qjv]], [[2nsp\|2nsp]], [[2nst\|2nst]], [[2nt6\|2nt6]], [[2nt9\|2nt9]], [[2ntb\|2ntb]], [[2ntp\|2ntp]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pemA, pem ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pemA, pem ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.11 3.1.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.11 3.1.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ntq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntq OCA], [http://pdbe.org/2ntq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ntq RCSB], [http://www.ebi.ac.uk/pdbsum/2ntq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ntq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2ntq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntq OCA], [http://pdbe.org/2ntq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ntq RCSB], [http://www.ebi.ac.uk/pdbsum/2ntq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ntq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:

	==See Also==		==See Also==
-	*[[Methylesterase\|Methylesterase]]	+	*[[Methylesterase 3D structures\|Methylesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 40:		Line 40:
	[[Category: Dicd3]]		[[Category: Dicd3]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
		+	[[Category: Large Structures]]
	[[Category: Brocklehurst, K]]		[[Category: Brocklehurst, K]]
	[[Category: Fries, M]]		[[Category: Fries, M]]

OCA at 16:48, 15 August 2018

2018-08-15T16:48:15Z

←Older revision		Revision as of 16:48, 15 August 2018
Line 1:		Line 1:
		+
	==Crystal structure of pectin methylesterase in complex with hexasaccharide VII==		==Crystal structure of pectin methylesterase in complex with hexasaccharide VII==
	<StructureSection load='2ntq' size='340' side='right' caption='[[2ntq]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2ntq' size='340' side='right' caption='[[2ntq]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pemA, pem ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pemA, pem ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.11 3.1.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.11 3.1.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ntq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntq OCA], [http://pdbe.org/2ntq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ntq RCSB], [http://www.ebi.ac.uk/pdbsum/2ntq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ntq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntq OCA], [http://pdbe.org/2ntq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ntq RCSB], [http://www.ebi.ac.uk/pdbsum/2ntq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ntq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/2ntq_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/2ntq_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 03:52, 7 February 2016

2016-02-07T03:52:29Z

←Older revision		Revision as of 03:52, 7 February 2016
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ntq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:32, 9 September 2015

2015-09-09T21:32:56Z

←Older revision		Revision as of 21:32, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='2ntq' size='340' side='right' caption='[[2ntq]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2ntq' size='340' side='right' caption='[[2ntq]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ntq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Erwinia_chrysanthemi Erwinia chrysanthemi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NTQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ntq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NTQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=M8C:METHYL+ALPHA-D-GALACTOPYRANURONATE'>M8C</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=M8C:METHYL+ALPHA-D-GALACTOPYRANURONATE'>M8C</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qjv\|1qjv]], [[2nsp\|2nsp]], [[2nst\|2nst]], [[2nt6\|2nt6]], [[2nt9\|2nt9]], [[2ntb\|2ntb]], [[2ntp\|2ntp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qjv\|1qjv]], [[2nsp\|2nsp]], [[2nst\|2nst]], [[2nt6\|2nt6]], [[2nt9\|2nt9]], [[2ntb\|2ntb]], [[2ntp\|2ntp]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pemA, pem ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~556 Erwinia chrysanthemi~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pemA, pem ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Pectinesterase Pectinesterase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.11 3.1.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.11 3.1.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ntq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ntq RCSB], [http://www.ebi.ac.uk/pdbsum/2ntq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ntq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntq OCA], [http://pdbe.org/2ntq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ntq RCSB], [http://www.ebi.ac.uk/pdbsum/2ntq PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ntq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Erwinia chrysanthemi~~]]	+	[[Category: Dicd3]]
-	[[Category: ~~Pectinesterase~~]]	+	[[Category: Hydrolase]]
	[[Category: Brocklehurst, K]]		[[Category: Brocklehurst, K]]
	[[Category: Fries, M]]		[[Category: Fries, M]]
	[[Category: Pickersgill, R W]]		[[Category: Pickersgill, R W]]
	[[Category: Shevchik, V E]]		[[Category: Shevchik, V E]]
-	[[Category: Hydrolase]]
	[[Category: Pme hexasaccharide complex]]		[[Category: Pme hexasaccharide complex]]

OCA at 07:40, 24 December 2014

2014-12-24T07:40:04Z

←Older revision		Revision as of 07:40, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ntq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NTQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ntq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NTQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=M8C:METHYL+ALPHA-D-GALACTOPYRANURONATE'>M8C</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=M8C:METHYL+ALPHA-D-GALACTOPYRANURONATE'>M8C</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qjv\|1qjv]], [[2nsp\|2nsp]], [[2nst\|2nst]], [[2nt6\|2nt6]], [[2nt9\|2nt9]], [[2ntb\|2ntb]], [[2ntp\|2ntp]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qjv\|1qjv]], [[2nsp\|2nsp]], [[2nst\|2nst]], [[2nt6\|2nt6]], [[2nt9\|2nt9]], [[2ntb\|2ntb]], [[2ntp\|2ntp]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pemA, pem ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pemA, pem ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pectinesterase Pectinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.11 3.1.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pectinesterase Pectinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.11 3.1.1.11] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ntq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ntq RCSB], [http://www.ebi.ac.uk/pdbsum/2ntq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ntq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ntq RCSB], [http://www.ebi.ac.uk/pdbsum/2ntq PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PMEA_DICD3 PMEA_DICD3]] Involved in maceration and soft-rotting of plant tissue.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	[[Category: Erwinia chrysanthemi]]		[[Category: Erwinia chrysanthemi]]
	[[Category: Pectinesterase]]		[[Category: Pectinesterase]]
-	[[Category: Brocklehurst, K.]]	+	[[Category: Brocklehurst, K]]
-	[[Category: Fries, M.]]	+	[[Category: Fries, M]]
-	[[Category: Pickersgill, R W.]]	+	[[Category: Pickersgill, R W]]
-	[[Category: Shevchik, V E.]]	+	[[Category: Shevchik, V E]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Pme hexasaccharide complex]]		[[Category: Pme hexasaccharide complex]]

OCA at 02:44, 29 September 2014

2014-09-29T02:44:50Z

←Older revision		Revision as of 02:44, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2ntq.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of pectin methylesterase in complex with hexasaccharide VII==
		+	<StructureSection load='2ntq' size='340' side='right' caption='[[2ntq]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ntq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NTQ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=M8C:METHYL+ALPHA-D-GALACTOPYRANURONATE'>M8C</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qjv\|1qjv]], [[2nsp\|2nsp]], [[2nst\|2nst]], [[2nt6\|2nt6]], [[2nt9\|2nt9]], [[2ntb\|2ntb]], [[2ntp\|2ntp]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pemA, pem ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pectinesterase Pectinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.11 3.1.1.11] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ntq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ntq RCSB], [http://www.ebi.ac.uk/pdbsum/2ntq PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/2ntq_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We provide a mechanism for the activity of pectin methylesterase (PME), the enzyme that catalyses the essential first step in bacterial invasion of plant tissues. The complexes formed in the crystal using specifically methylated pectins, together with kinetic measurements of directed mutants, provide clear insights at atomic resolution into the specificity and the processive action of the Erwinia chrysanthemi enzyme. Product complexes provide additional snapshots along the reaction coordinate. We previously revealed that PME is a novel aspartic-esterase possessing parallel beta-helix architecture and now show that the two conserved aspartates are the nucleophile and general acid-base in the mechanism, respectively. Other conserved residues at the catalytic centre are shown to be essential for substrate binding or transition state stabilisation. The preferential binding of methylated sugar residues upstream of the catalytic site, and demethylated residues downstream, drives the enzyme along the pectin molecule and accounts for the sequential pattern of demethylation produced by both bacterial and plant PMEs.

-	~~{{STRUCTURE_2ntq\| PDB=2ntq \| SCENE= }}~~	+	Molecular basis of the activity of the phytopathogen pectin methylesterase.,Fries M, Ihrig J, Brocklehurst K, Shevchik VE, Pickersgill RW EMBO J. 2007 Sep 5;26(17):3879-87. Epub 2007 Aug 23. PMID:17717531<ref>PMID:17717531</ref>

-	~~===Crystal structure~~ of ~~pectin methylesterase in complex with hexasaccharide VII===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17717531}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2ntq]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA].~~	+

	==See Also==		==See Also==
	*[[Methylesterase\|Methylesterase]]		*[[Methylesterase\|Methylesterase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:017717531<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Erwinia chrysanthemi]]		[[Category: Erwinia chrysanthemi]]
	[[Category: Pectinesterase]]		[[Category: Pectinesterase]]

OCA at 05:31, 20 October 2012

2012-10-20T05:31:05Z

←Older revision		Revision as of 05:31, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2ntq]] is a 2 chain structure ~~of [[Methylesterase]]~~ with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA].	+	[[2ntq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA].

	==See Also==		==See Also==

OCA: Protected "2ntq" [edit=sysop:move=sysop]

2012-07-25T10:18:49Z

Protected "2ntq" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:18, 25 July 2012

←Older revision		Revision as of 11:37, 16 February 2022
Line 3:		Line 3:
	<StructureSection load='2ntq' size='340' side='right'caption='[[2ntq]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2ntq' size='340' side='right'caption='[[2ntq]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ntq]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2NTQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ntq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NTQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=M8C:METHYL+ALPHA-D-GALACTOPYRANURONATE'>M8C</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=M8C:METHYL+ALPHA-D-GALACTOPYRANURONATE'>M8C</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qjv\|1qjv]], [[2nsp\|2nsp]], [[2nst\|2nst]], [[2nt6\|2nt6]], [[2nt9\|2nt9]], [[2ntb\|2ntb]], [[2ntp\|2ntp]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1qjv\|1qjv]], [[2nsp\|2nsp]], [[2nst\|2nst]], [[2nt6\|2nt6]], [[2nt9\|2nt9]], [[2ntb\|2ntb]], [[2ntp\|2ntp]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pemA, pem ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pemA, pem ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.11 3.1.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.11 3.1.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2ntq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntq OCA], [~~http~~://pdbe.org/2ntq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ntq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ntq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ntq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ntq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntq OCA], [https://pdbe.org/2ntq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ntq RCSB], [https://www.ebi.ac.uk/pdbsum/2ntq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ntq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PMEA_DICD3 PMEA_DICD3]] Involved in maceration and soft-rotting of plant tissue.	+	[[https://www.uniprot.org/uniprot/PMEA_DICD3 PMEA_DICD3]] Involved in maceration and soft-rotting of plant tissue.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]