2oar - Revision history

OCA at 00:16, 28 December 2023

2023-12-28T00:16:21Z

←Older revision		Revision as of 00:16, 28 December 2023
Line 3:		Line 3:
	<StructureSection load='2oar' size='340' side='right'caption='[[2oar]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='2oar' size='340' side='right'caption='[[2oar]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2oar]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Mycta Mycta~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. The November 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Mechanosensitive Channels'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_11 10.2210/rcsb_pdb/mom_2008_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OAR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2oar]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Ra Mycobacterium tuberculosis H37Ra]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. The November 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Mechanosensitive Channels'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_11 10.2210/rcsb_pdb/mom_2008_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OAR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=AU:GOLD+ION'>AU</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.5Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~mscL ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 MYCTA])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oar OCA], [https://pdbe.org/2oar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oar RCSB], [https://www.ebi.ac.uk/pdbsum/2oar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oar ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oar OCA], [https://pdbe.org/2oar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oar RCSB], [https://www.ebi.ac.uk/pdbsum/2oar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oar ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~MSCL_MYCTU MSCL_MYCTU]~~] Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell ~~(By similarity)~~.	+	[https://www.uniprot.org/uniprot/MSCL_MYCTA MSCL_MYCTA] Channel that opens in response to stretch forces in the membrane lipid bilayer. The force required to trigger channel opening depends on the nature of the membrane lipids; the presence of phosphatidylinositol enhances mechanosensitivity of the channel. May participate in the regulation of osmotic pressure changes within the cell.[UniProtKB:P9WJN5]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oar ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oar ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Mechanosensitive ion channels play a critical role in transducing physical stresses at the cell membrane into an electrochemical response. The MscL family of large-conductance mechanosensitive channels is widely distributed among prokaryotes and may participate in the regulation of osmotic pressure changes within the cell. In an effort to better understand the structural basis for the function of these channels, the structure of the MscL homolog from Mycobacterium tuberculosis was determined by x-ray crystallography to 3.5 angstroms resolution. This channel is organized as a homopentamer, with each subunit containing two transmembrane alpha helices and a third cytoplasmic alpha helix. From the extracellular side, a water-filled opening approximately 18 angstroms in diameter leads into a pore lined with hydrophilic residues which narrows at the cytoplasmic side to an occluded hydrophobic apex that may act as the channel gate. This structure may serve as a model for other mechanosensitive channels, as well as the broader class of pentameric ligand-gated ion channels exemplified by the nicotinic acetylcholine receptor.
-
-	Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel.,Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC Science. 1998 Dec 18;282(5397):2220-6. PMID:9856938<ref>PMID:9856938</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2oar" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ion channels 3D structures\|Ion channels 3D structures]]		*[[Ion channels 3D structures\|Ion channels 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Mechanosensitive Channels]]		[[Category: Mechanosensitive Channels]]
-	[[Category: ~~Mycta~~]]	+	[[Category: Mycobacterium tuberculosis H37Ra]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: Chang, G]]	+	[[Category: Chang G]]
-	[[Category: Lee~~, A T~~]]	+	[[Category: Lee AT]]
-	[[Category: Rees~~, D C~~]]	+	[[Category: Rees DC]]
-	[[Category: Spencer~~, R H~~]]	+	[[Category: Spencer RH]]
-	[[Category: Steinbacher, S]]	+	[[Category: Steinbacher S]]
-	[[Category: Strop, P]]	+	[[Category: Strop P]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Stretch activated ion channel mechanosensitive~~]]	+

OCA at 12:51, 9 June 2021

2021-06-09T12:51:16Z

←Older revision		Revision as of 12:51, 9 June 2021
Line 1:		Line 1:

	==Mechanosensitive Channel of Large Conductance (MscL)==		==Mechanosensitive Channel of Large Conductance (MscL)==
-	<StructureSection load='2oar' size='340' side='right' caption='[[2oar]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<StructureSection load='2oar' size='340' side='right'caption='[[2oar]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2oar]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Mycta Mycta]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. The November 2008 RCSB PDB [~~http~~://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Mechanosensitive Channels'' by David Goodsell is [~~http~~://dx.doi.org/10.2210/rcsb_pdb/mom_2008_11 10.2210/rcsb_pdb/mom_2008_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2OAR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2oar]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycta Mycta]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. The November 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Mechanosensitive Channels'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_11 10.2210/rcsb_pdb/mom_2008_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OAR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mscL ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 MYCTA])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mscL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 MYCTA])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oar OCA], [~~http~~://pdbe.org/2oar PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2oar RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2oar PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2oar ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oar OCA], [https://pdbe.org/2oar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oar RCSB], [https://www.ebi.ac.uk/pdbsum/2oar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oar ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MSCL_MYCTU MSCL_MYCTU]] Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell (By similarity).	+	[[https://www.uniprot.org/uniprot/MSCL_MYCTU MSCL_MYCTU]] Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Ion channels\|Ion channels]]	+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
-	*[[Mechanosensitive channels: opening and closing\|Mechanosensitive channels: opening and closing]]	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Mechanosensitive Channels]]		[[Category: Mechanosensitive Channels]]
	[[Category: Mycta]]		[[Category: Mycta]]

OCA at 09:23, 26 December 2018

2018-12-26T09:23:42Z

←Older revision		Revision as of 09:23, 26 December 2018
Line 1:		Line 1:
-	{{Seed}}
-	[[Image:2oar.png\|left\|200px]]

	==Mechanosensitive Channel of Large Conductance (MscL)==		==Mechanosensitive Channel of Large Conductance (MscL)==
		+	<StructureSection load='2oar' size='340' side='right' caption='[[2oar]], [[Resolution\|resolution]] 3.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2oar]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycta Mycta]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. The November 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Mechanosensitive Channels'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_11 10.2210/rcsb_pdb/mom_2008_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OAR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mscL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 MYCTA])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oar OCA], [http://pdbe.org/2oar PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2oar RCSB], [http://www.ebi.ac.uk/pdbsum/2oar PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2oar ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MSCL_MYCTU MSCL_MYCTU]] Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell (By similarity).
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/2oar_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oar ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mechanosensitive ion channels play a critical role in transducing physical stresses at the cell membrane into an electrochemical response. The MscL family of large-conductance mechanosensitive channels is widely distributed among prokaryotes and may participate in the regulation of osmotic pressure changes within the cell. In an effort to better understand the structural basis for the function of these channels, the structure of the MscL homolog from Mycobacterium tuberculosis was determined by x-ray crystallography to 3.5 angstroms resolution. This channel is organized as a homopentamer, with each subunit containing two transmembrane alpha helices and a third cytoplasmic alpha helix. From the extracellular side, a water-filled opening approximately 18 angstroms in diameter leads into a pore lined with hydrophilic residues which narrows at the cytoplasmic side to an occluded hydrophobic apex that may act as the channel gate. This structure may serve as a model for other mechanosensitive channels, as well as the broader class of pentameric ligand-gated ion channels exemplified by the nicotinic acetylcholine receptor.

-	~~===Model~~ of ~~Channel Opening===~~	+	Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel.,Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC Science. 1998 Dec 18;282(5397):2220-6. PMID:9856938<ref>PMID:9856938</ref>
-	~~<applet load='1kyk' size='300' frame='true' align='right' caption='Theoretical Models'~~	+
-	~~scene='Goodsell_Sandbox/1kyl/1' />~~	+
-	~~Theoretical models<ref>The theoretical models were deposited in~~ the [[PDB]] (before it stopped accepting theoretical models) as [[1kyk]], [[1kyl]], and [[1kym]].</ref> of the <scene name='Goodsell_Sandbox/1kyk/1'>closed</scene> (initial scene), <scene name='Goodsell_Sandbox/1kyl/1'>intermediate</scene>, and <scene name='Goodsell_Sandbox/1kym/1'>open</scene> forms of the channel have been generated based on data from ~~electron paramagnetic resonance spectroscopy, using mutant forms of the protein with spin labels attached to engineered cysteine amino acids in the transmembrane segments<ref>Open~~ channel ~~structure of MscL and the gating mechanism of mechanosensitive channels~~. ~~Perozo~~, E, ~~Cortes DM~~, ~~Sompornpisut~~, P, ~~Kloda, A, Martinac, B~~. ~~Nature 2002, 418, 942~~-~~948~~. PMID:~~[http~~:~~//www.ncbi.nlm.nih.gov/pubmed/12198539 12198539]~~</ref>.	+

-	~~{{Clear}}~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~----~~	+	</div>
-	<br>	+	<div class="pdbe-citations 2oar" style="background-color:#fffaf0;"></div>
-	<~~!--~~	+
-	~~The FIRST line below this paragraph, containing "STRUCTURE_2oar", creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-		+
-	~~The SECOND line below this paragraph, {{ABSTRACT_PUBMED_9856938}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at http:~~/~~/www.pubmed.gov), where 9856938 is the PubMed ID number.~~	+
-	-->	+
-	~~{{STRUCTURE_2oar\| PDB~~=2oar ~~\| SCENE~~= }}	+
-	~~{{ABSTRACT_PUBMED_9856938}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~2OAR <ref>Structure of the MscL homolog from Mycobacterium tuberculosis~~: ~~a gated mechanosensitive ion channel., Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC, Science. 1998 Dec 18~~;~~282(5397):2220-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9856938 9856938]~~</~~ref~~> is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA].	+

	==See Also==		==See Also==
-	~~Many structural features of a related channel, MscS, including animated morphs of opening an closing, are illustrated at~~ [[Mechanosensitive channels: opening and closing]].	+	*[[Ion channels\|Ion channels]]
-		+	*[[Mechanosensitive channels: opening and closing\|Mechanosensitive channels: opening and closing]]
-	==References ~~and Notes~~==	+	== References ==
	<references/>		<references/>
-	[[Category: ~~Mycobacterium tuberculosis~~]]	+	__TOC__
-	[[Category: ~~Single protein~~]]	+	</StructureSection>
-	[[Category: Chang, G.]]	+	[[Category: Mechanosensitive Channels]]
-	[[Category: Lee, A T.]]	+	[[Category: Mycta]]
-	[[Category: Rees, D C.]]	+	[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: Spencer, R H.]]	+	[[Category: Chang, G]]
-	[[Category: Steinbacher, S.]]	+	[[Category: Lee, A T]]
-	[[Category: Strop, P.]]	+	[[Category: Rees, D C]]
		+	[[Category: Spencer, R H]]
		+	[[Category: Steinbacher, S]]
		+	[[Category: Strop, P]]
		+	[[Category: Membrane protein]]
	[[Category: Stretch activated ion channel mechanosensitive]]		[[Category: Stretch activated ion channel mechanosensitive]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 20 12:12:19 2008''

OCA: Protected "2oar" [edit=sysop:move=sysop]

2017-03-22T17:03:40Z

Protected "2oar" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:03, 22 March 2017

Eric Martz: /* About this Structure */ Added link to Mechanosensitive channels under See Also

2009-03-10T22:06:16Z

About this Structure - Added link to Mechanosensitive channels under See Also

←Older revision		Revision as of 22:06, 10 March 2009
Line 27:		Line 27:
	2OAR <ref>Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel., Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC, Science. 1998 Dec 18;282(5397):2220-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9856938 9856938]</ref> is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA].		2OAR <ref>Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel., Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC, Science. 1998 Dec 18;282(5397):2220-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9856938 9856938]</ref> is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA].

		+	==See Also==
		+	Many structural features of a related channel, MscS, including animated morphs of opening an closing, are illustrated at [[Mechanosensitive channels: opening and closing]].

	==References and Notes==		==References and Notes==

Eric Martz: /* Model of Channel Opening */ polishing

2008-11-21T22:19:14Z

Model of Channel Opening - polishing

←Older revision		Revision as of 22:19, 21 November 2008
Line 7:		Line 7:
	<applet load='1kyk' size='300' frame='true' align='right' caption='Theoretical Models'		<applet load='1kyk' size='300' frame='true' align='right' caption='Theoretical Models'
	scene='Goodsell_Sandbox/1kyl/1' />		scene='Goodsell_Sandbox/1kyl/1' />
-	Theoretical models<ref>The theoretical models were deposited in the [[PDB]] (before it stopped accepting theoretical models) as [[1kyk]], [[1kyl]], and [[1kym]].</ref> of the <scene name='Goodsell_Sandbox/1kyk/1'>closed, </scene><scene name='Goodsell_Sandbox/1kyl/1'>intermediate,</scene> and <scene name='Goodsell_Sandbox/1kym/1'>open</scene> forms of the channel have been generated based on data from electron paramagnetic resonance spectroscopy, using mutant forms of the protein with spin labels attached to engineered cysteine amino acids in the transmembrane segments<ref>Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Perozo, E, Cortes DM, Sompornpisut, P, Kloda, A, Martinac, B. Nature 2002, 418, 942-948. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12198539 12198539]</ref>.	+	Theoretical models<ref>The theoretical models were deposited in the [[PDB]] (before it stopped accepting theoretical models) as [[1kyk]], [[1kyl]], and [[1kym]].</ref> of the <scene name='Goodsell_Sandbox/1kyk/1'>closed</scene> (initial scene), <scene name='Goodsell_Sandbox/1kyl/1'>intermediate</scene>, and <scene name='Goodsell_Sandbox/1kym/1'>open</scene> forms of the channel have been generated based on data from electron paramagnetic resonance spectroscopy, using mutant forms of the protein with spin labels attached to engineered cysteine amino acids in the transmembrane segments<ref>Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Perozo, E, Cortes DM, Sompornpisut, P, Kloda, A, Martinac, B. Nature 2002, 418, 942-948. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12198539 12198539]</ref>.

	{{Clear}}		{{Clear}}

Eric Martz: Moved theoretical models to a separate Jmol

2008-11-21T22:14:46Z

Moved theoretical models to a separate Jmol

←Older revision		Revision as of 22:14, 21 November 2008
Line 2:		Line 2:
	[[Image:2oar.png\|left\|200px]]		[[Image:2oar.png\|left\|200px]]

		+	==Mechanosensitive Channel of Large Conductance (MscL)==
		+
		+	===Model of Channel Opening===
		+	<applet load='1kyk' size='300' frame='true' align='right' caption='Theoretical Models'
		+	scene='Goodsell_Sandbox/1kyl/1' />
		+	Theoretical models<ref>The theoretical models were deposited in the [[PDB]] (before it stopped accepting theoretical models) as [[1kyk]], [[1kyl]], and [[1kym]].</ref> of the <scene name='Goodsell_Sandbox/1kyk/1'>closed, </scene><scene name='Goodsell_Sandbox/1kyl/1'>intermediate,</scene> and <scene name='Goodsell_Sandbox/1kym/1'>open</scene> forms of the channel have been generated based on data from electron paramagnetic resonance spectroscopy, using mutant forms of the protein with spin labels attached to engineered cysteine amino acids in the transmembrane segments<ref>Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Perozo, E, Cortes DM, Sompornpisut, P, Kloda, A, Martinac, B. Nature 2002, 418, 942-948. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12198539 12198539]</ref>.
		+
		+	{{Clear}}
		+	----
		+	<br>
	<!--		<!--
-	The line below this paragraph, containing "STRUCTURE_2oar", creates the "Structure Box" on the page.	+	The FIRST line below this paragraph, containing "STRUCTURE_2oar", creates the "Structure Box" on the page.
	You may change the PDB parameter (which sets the PDB file loaded into the applet)		You may change the PDB parameter (which sets the PDB file loaded into the applet)
	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),		or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
	or leave the SCENE parameter empty for the default display.		or leave the SCENE parameter empty for the default display.
-	-->
-	{{STRUCTURE_2oar\| PDB=2oar \| SCENE= }}
-
-	===Mechanosensitive Channel of Large Conductance (MscL)===
-
-	==Model of Channel Opening==
-	Theoretical models<ref>The theoretical models were deposited in the [[PDB]] (before it stopped accepting theoretical models) as [[1kyk]], [[1kyl]], and [[1kym]].</ref> the <scene name='Goodsell_Sandbox/1kyk/1'>closed, </scene><scene name='Goodsell_Sandbox/1kyl/1'>intermediate,</scene> and <scene name='Goodsell_Sandbox/1kym/1'>open</scene> forms of the channel have been generated based on data from electron paramagnetic resonance spectroscopy, using mutant forms of the protein with spin labels attached to engineered cysteine amino acids in the transmembrane segments<ref>Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Perozo, E, Cortes DM, Sompornpisut, P, Kloda, A, Martinac, B. Nature 2002, 418, 942-948. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12198539 12198539]</ref>.

-	~~<!--~~	+	The SECOND line below this paragraph, {{ABSTRACT_PUBMED_9856938}}, adds the Publication Abstract to the page
-	The line below this paragraph, {{ABSTRACT_PUBMED_9856938}}, adds the Publication Abstract to the page	+
	(as it appears on PubMed at http://www.pubmed.gov), where 9856938 is the PubMed ID number.		(as it appears on PubMed at http://www.pubmed.gov), where 9856938 is the PubMed ID number.
	-->		-->
		+	{{STRUCTURE_2oar\| PDB=2oar \| SCENE= }}
	{{ABSTRACT_PUBMED_9856938}}		{{ABSTRACT_PUBMED_9856938}}

Eric Martz: /* Reference */ polishing

2008-11-21T22:05:24Z

Reference - polishing

←Older revision		Revision as of 22:05, 21 November 2008
Line 25:		Line 25:


-	==~~Reference~~==	+	==References and Notes==
	<references/>		<references/>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]

Eric Martz: /* Model of Channel Opening */ added footnote about provenance of theoretical models

2008-11-21T22:04:46Z

Model of Channel Opening - added footnote about provenance of theoretical models

←Older revision		Revision as of 22:04, 21 November 2008
Line 13:		Line 13:

	==Model of Channel Opening==		==Model of Channel Opening==
-	~~Models~~ the <scene name='Goodsell_Sandbox/1kyk/1'>closed, </scene><scene name='Goodsell_Sandbox/1kyl/1'>intermediate,</scene> and <scene name='Goodsell_Sandbox/1kym/1'>open</scene> forms of the channel have been generated based on data from electron paramagnetic resonance spectroscopy, using mutant forms of the protein with spin labels attached to engineered cysteine amino acids in the transmembrane segments<ref>Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Perozo, E, Cortes DM, Sompornpisut, P, Kloda, A, Martinac, B. Nature 2002, 418, 942-948. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12198539 12198539]</ref>.	+	Theoretical models<ref>The theoretical models were deposited in the [[PDB]] (before it stopped accepting theoretical models) as [[1kyk]], [[1kyl]], and [[1kym]].</ref> the <scene name='Goodsell_Sandbox/1kyk/1'>closed, </scene><scene name='Goodsell_Sandbox/1kyl/1'>intermediate,</scene> and <scene name='Goodsell_Sandbox/1kym/1'>open</scene> forms of the channel have been generated based on data from electron paramagnetic resonance spectroscopy, using mutant forms of the protein with spin labels attached to engineered cysteine amino acids in the transmembrane segments<ref>Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Perozo, E, Cortes DM, Sompornpisut, P, Kloda, A, Martinac, B. Nature 2002, 418, 942-948. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12198539 12198539]</ref>.

	<!--		<!--

Eran Hodis at 18:37, 9 November 2008

2008-11-09T18:37:47Z

←Older revision		Revision as of 18:37, 9 November 2008
Line 12:		Line 12:
	===Mechanosensitive Channel of Large Conductance (MscL)===		===Mechanosensitive Channel of Large Conductance (MscL)===

		+	==Model of Channel Opening==
		+	Models the <scene name='Goodsell_Sandbox/1kyk/1'>closed, </scene><scene name='Goodsell_Sandbox/1kyl/1'>intermediate,</scene> and <scene name='Goodsell_Sandbox/1kym/1'>open</scene> forms of the channel have been generated based on data from electron paramagnetic resonance spectroscopy, using mutant forms of the protein with spin labels attached to engineered cysteine amino acids in the transmembrane segments<ref>Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Perozo, E, Cortes DM, Sompornpisut, P, Kloda, A, Martinac, B. Nature 2002, 418, 942-948. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12198539 12198539]</ref>.

	<!--		<!--
Line 22:		Line 24:
	2OAR <ref>Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel., Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC, Science. 1998 Dec 18;282(5397):2220-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9856938 9856938]</ref> is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA].		2OAR <ref>Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel., Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC, Science. 1998 Dec 18;282(5397):2220-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9856938 9856938]</ref> is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA].

-	==Model of Channel Opening==
-	Models the <scene name='Goodsell_Sandbox/1kyk/1'>closed, </scene><scene name='Goodsell_Sandbox/1kyl/1'>intermediate,</scene> and <scene name='Goodsell_Sandbox/1kym/1'>open</scene> forms of the channel have been generated based on data from electron paramagnetic resonance spectroscopy, using mutant forms of the protein with spin labels attached to engineered cysteine amino acids in the transmembrane segments<ref>Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Perozo, E, Cortes DM, Sompornpisut, P, Kloda, A, Martinac, B. Nature 2002, 418, 942-948. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12198539 12198539]</ref>.

	==Reference==		==Reference==