2og0 - Revision history

OCA at 13:09, 26 July 2023

2023-07-26T13:09:57Z

←Older revision		Revision as of 13:09, 26 July 2023
Line 3:		Line 3:
	<StructureSection load='2og0' size='340' side='right'caption='[[2og0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2og0' size='340' side='right'caption='[[2og0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2og0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bacteriophage_lambda Bacteriophage lambda~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OG0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2og0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OG0 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~xis (~~[~~https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=10710 Bacteriophage lambda])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2og0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2og0 OCA], [https://pdbe.org/2og0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2og0 RCSB], [https://www.ebi.ac.uk/pdbsum/2og0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2og0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2og0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2og0 OCA], [https://pdbe.org/2og0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2og0 RCSB], [https://www.ebi.ac.uk/pdbsum/2og0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2og0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD]] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site.	+	[https://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacteriophage lambda~~]]	+	[[Category: Escherichia virus Lambda]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abbani~~, M A~~]]	+	[[Category: Abbani MA]]
-	[[Category: Cascio, D]]	+	[[Category: Cascio D]]
-	[[Category: Clubb~~, R T~~]]	+	[[Category: Clubb RT]]
-	[[Category: Johnson~~, R C~~]]	+	[[Category: Johnson RC]]
-	[[Category: Papagiannis~~, C V~~]]	+	[[Category: Papagiannis CV]]
-	[[Category: Sam~~, M D~~]]	+	[[Category: Sam MD]]
-	[[Category: Yoo, D]]	+	[[Category: Yoo D]]
-	~~[[Category: Winged helix protein]]~~	+
-	~~[[Category: Dna architectural protein]]~~	+
-	~~[[Category: Dna binding protein-dna complex]]~~	+
-	~~[[Category: Phage excision]]~~	+
-	~~[[Category: Protein-dna complex]]~~	+
-	~~[[Category: Site-specific recombination recombination~~]]	+

OCA at 12:55, 9 June 2021

2021-06-09T12:55:31Z

←Older revision		Revision as of 12:55, 9 June 2021
Line 1:		Line 1:

	==Crystal Structure of the Lambda Xis-DNA complex==		==Crystal Structure of the Lambda Xis-DNA complex==
-	<StructureSection load='2og0' size='340' side='right' caption='[[2og0]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='2og0' size='340' side='right'caption='[[2og0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2og0]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2OG0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2og0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OG0 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xis ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xis ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2og0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2og0 OCA], [~~http~~://pdbe.org/2og0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2og0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2og0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2og0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2og0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2og0 OCA], [https://pdbe.org/2og0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2og0 RCSB], [https://www.ebi.ac.uk/pdbsum/2og0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2og0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD]] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site.	+	[[https://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD]] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Bacteriophage lambda]]		[[Category: Bacteriophage lambda]]
		+	[[Category: Large Structures]]
	[[Category: Abbani, M A]]		[[Category: Abbani, M A]]
	[[Category: Cascio, D]]		[[Category: Cascio, D]]

OCA at 08:59, 18 October 2017

2017-10-18T08:59:52Z

←Older revision		Revision as of 08:59, 18 October 2017
Line 1:		Line 1:
		+
	==Crystal Structure of the Lambda Xis-DNA complex==		==Crystal Structure of the Lambda Xis-DNA complex==
	<StructureSection load='2og0' size='340' side='right' caption='[[2og0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2og0' size='340' side='right' caption='[[2og0]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2og0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OG0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2og0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OG0 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xis ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xis ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2og0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2og0 OCA], [http://pdbe.org/2og0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2og0 RCSB], [http://www.ebi.ac.uk/pdbsum/2og0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2og0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2og0 OCA], [http://pdbe.org/2og0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2og0 RCSB], [http://www.ebi.ac.uk/pdbsum/2og0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2og0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 21:41, 10 September 2015

2015-09-10T21:41:40Z

←Older revision		Revision as of 21:41, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2og0' size='340' side='right' caption='[[2og0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2og0' size='340' side='right' caption='[[2og0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2og0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_lambda Enterobacteria phage~~ lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OG0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2og0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OG0 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xis ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 ~~Enterobacteria phage~~ lambda])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xis ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2og0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2og0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2og0 RCSB], [http://www.ebi.ac.uk/pdbsum/2og0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2og0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2og0 OCA], [http://pdbe.org/2og0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2og0 RCSB], [http://www.ebi.ac.uk/pdbsum/2og0 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2og0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacteria phage~~ lambda]]	+	[[Category: Bacteriophage lambda]]
	[[Category: Abbani, M A]]		[[Category: Abbani, M A]]
	[[Category: Cascio, D]]		[[Category: Cascio, D]]

OCA at 06:28, 25 December 2014

2014-12-25T06:28:39Z

←Older revision		Revision as of 06:28, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2og0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2og0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2og0 RCSB], [http://www.ebi.ac.uk/pdbsum/2og0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2og0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2og0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2og0 RCSB], [http://www.ebi.ac.uk/pdbsum/2og0 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD]] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacteria phage lambda]]		[[Category: Enterobacteria phage lambda]]
-	[[Category: Abbani, M A.]]	+	[[Category: Abbani, M A]]
-	[[Category: Cascio, D.]]	+	[[Category: Cascio, D]]
-	[[Category: Clubb, R T.]]	+	[[Category: Clubb, R T]]
-	[[Category: Johnson, R C.]]	+	[[Category: Johnson, R C]]
-	[[Category: Papagiannis, C V.]]	+	[[Category: Papagiannis, C V]]
-	[[Category: Sam, M D.]]	+	[[Category: Sam, M D]]
-	[[Category: Yoo, D.]]	+	[[Category: Yoo, D]]
	[[Category: Winged helix protein]]		[[Category: Winged helix protein]]
	[[Category: Dna architectural protein]]		[[Category: Dna architectural protein]]

OCA at 11:29, 20 October 2014

2014-10-20T11:29:56Z

←Older revision		Revision as of 11:29, 20 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2og0.~~png~~\|~~left\|200px~~]]	+	==Crystal Structure of the Lambda Xis-DNA complex==
		+	<StructureSection load='2og0' size='340' side='right' caption='[[2og0]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2og0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OG0 FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">xis ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2og0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2og0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2og0 RCSB], [http://www.ebi.ac.uk/pdbsum/2og0 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The phage-encoded Xis protein is the major determinant controlling the direction of recombination in phage lambda. Xis is a winged-helix DNA binding protein that cooperatively binds to the attR recombination site to generate a curved microfilament, which promotes assembly of the excisive intasome but inhibits formation of an integrative intasome. We find that lambda synthesizes surprisingly high levels of Xis immediately upon prophage induction when excision rates are maximal. However, because of its low sequence-specific binding activity, exemplified by a 1.9 A co-crystal structure of a non-specifically bound DNA complex, Xis is relatively ineffective at promoting excision in vivo in the absence of the host Fis protein. Fis binds to a segment in attR that almost entirely overlaps one of the Xis binding sites. Instead of sterically excluding Xis binding from this site, as has been previously believed, we show that Fis enhances binding of all three Xis protomers to generate the microfilament. A specific Fis-Xis interface is supported by the effects of mutations within each protein, and relaxed, but not completely sequence-neutral, binding by the central Xis protomer is supported by the effects of DNA mutations. We present a structural model for the 50 bp curved Fis-Xis cooperative complex that is assembled between the arm and core Int binding sites whose trajectory places constraints on models for the excisive intasome structure.

-	~~{{STRUCTURE_2og0\| PDB=2og0 \| SCENE= }}~~	+	Fis targets assembly of the Xis nucleoprotein filament to promote excisive recombination by phage lambda.,Papagiannis CV, Sam MD, Abbani MA, Yoo D, Cascio D, Clubb RT, Johnson RC J Mol Biol. 2007 Mar 23;367(2):328-43. Epub 2007 Jan 3. PMID:17275024<ref>PMID:17275024</ref>

-	~~===Crystal Structure~~ of the ~~Lambda Xis-DNA complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17275024}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[2og0]] is a 4 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA].~~	+	</StructureSection>
	[[Category: Enterobacteria phage lambda]]		[[Category: Enterobacteria phage lambda]]
	[[Category: Abbani, M A.]]		[[Category: Abbani, M A.]]

OCA: Protected "2og0" [edit=sysop:move=sysop]

2013-01-07T15:00:44Z

Protected "2og0" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:00, 7 January 2013

OCA at 15:00, 7 January 2013

2013-01-07T15:00:41Z

←Older revision		Revision as of 15:00, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2og0.png\|left\|200px]]		[[Image:2og0.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2og0", creates the "Structure Box" on the page.
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-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_2og0\| PDB=2og0 \| SCENE= }}		{{STRUCTURE_2og0\| PDB=2og0 \| SCENE= }}

	===Crystal Structure of the Lambda Xis-DNA complex===		===Crystal Structure of the Lambda Xis-DNA complex===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17275024}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17275024 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17275024}}		{{ABSTRACT_PUBMED_17275024}}

	==About this Structure==		==About this Structure==
-	~~2OG0 is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA].	+	[[2og0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OG0 OCA].
-		+
-	~~==Reference==~~	+
-	Fis targets assembly of the Xis nucleoprotein filament to promote excisive recombination by phage lambda., Papagiannis CV, Sam MD, Abbani MA, Yoo D, Cascio D, Clubb RT, Johnson RC, J Mol Biol. 2007 Mar 23;367(2):328-43. Epub 2007 Jan 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17275024 17275024]	+
	[[Category: Enterobacteria phage lambda]]		[[Category: Enterobacteria phage lambda]]
-	[[Category: Single protein]]
	[[Category: Abbani, M A.]]		[[Category: Abbani, M A.]]
	[[Category: Cascio, D.]]		[[Category: Cascio, D.]]
Line 35:		Line 19:
	[[Category: Winged helix protein]]		[[Category: Winged helix protein]]
	[[Category: Dna architectural protein]]		[[Category: Dna architectural protein]]
		+	[[Category: Dna binding protein-dna complex]]
	[[Category: Phage excision]]		[[Category: Phage excision]]
	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]
	[[Category: Site-specific recombination recombination]]		[[Category: Site-specific recombination recombination]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:56:07 2008''

OCA at 09:56, 28 July 2008

2008-07-28T09:56:09Z

←Older revision		Revision as of 09:56, 28 July 2008
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-	[[Image:2og0.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2og0.png\|left\|200px]]

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	{{STRUCTURE_2og0\| PDB=2og0 \| SCENE= }}		{{STRUCTURE_2og0\| PDB=2og0 \| SCENE= }}

-	~~'''~~Crystal Structure of the Lambda Xis-DNA complex~~'''~~	+	===Crystal Structure of the Lambda Xis-DNA complex===


-	~~==Overview==~~	+	<!--
-	The phage-encoded Xis protein is the major determinant controlling the direction of recombination in phage lambda. Xis is a winged-helix DNA binding protein that cooperatively binds to the attR recombination site to generate a curved microfilament, which promotes assembly of the excisive intasome but inhibits formation of an integrative intasome. We find that lambda synthesizes surprisingly high levels of Xis immediately upon prophage induction when excision rates are maximal. However, because of its low sequence-specific binding activity, exemplified by a 1.9 A co-crystal structure of a non-specifically bound DNA complex, Xis is relatively ineffective at promoting excision in vivo in the ~~absence of the host Fis protein. Fis binds~~ to ~~a segment in attR that almost entirely overlaps one of~~ the ~~Xis binding sites. Instead of sterically excluding Xis binding from this site,~~ as ~~has been previously believed, we show that Fis enhances binding of all three Xis protomers to generate the microfilament~~. ~~A specific Fis-Xis interface is supported by the effects of mutations within each protein~~, ~~and relaxed, but not completely sequence-neutral, binding by the central Xis protomer~~ is ~~supported by~~ the ~~effects of DNA mutations~~. ~~We present a structural model for the 50 bp curved Fis~~-~~Xis cooperative complex that is assembled between the arm and core Int binding sites whose trajectory places constraints on models for the excisive intasome structure.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_17275024}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17275024 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_17275024}}

	==About this Structure==		==About this Structure==
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	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]
	[[Category: Site-specific recombination recombination]]		[[Category: Site-specific recombination recombination]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 10~~:49:41 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:56:07 2008''

OCA at 07:49, 4 May 2008

2008-05-04T07:49:43Z

←Older revision		Revision as of 07:49, 4 May 2008
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	[[Image:2og0.gif\|left\|200px]]		[[Image:2og0.gif\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 2og0 \|SIZE=350\|CAPTION=~~ <~~scene name='initialview01'>2og0</scene>, resolution 1.900Å~~	+	The line below this paragraph, containing "STRUCTURE_2og0", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <scene name='pdbligand=DA:2'~~-~~DEOXYADENOSINE~~-~~5'-MONOPHOSPHATE'>DA</scene>~~, ~~<scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>~~, ~~<scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY=~~	+	or leave the SCENE parameter empty for the default display.
-	~~\|GENE= xis~~ (~~[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda]~~)	+	-->
-	~~\|DOMAIN=~~	+	{{STRUCTURE_2og0\| PDB=2og0 \| SCENE= }}
-	~~\|RELATEDENTRY=~~	+
-	\|~~RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol~~=2og0 ~~FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id~~=~~2og0 OCA], [http://www.ebi.ac.uk/pdbsum/2og0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2og0 RCSB]</span>~~	+
-	}}	+

	'''Crystal Structure of the Lambda Xis-DNA complex'''		'''Crystal Structure of the Lambda Xis-DNA complex'''
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	[[Category: Sam, M D.]]		[[Category: Sam, M D.]]
	[[Category: Yoo, D.]]		[[Category: Yoo, D.]]
-	[[Category: ~~winged~~ helix protein]]	+	[[Category: Winged helix protein]]
-	[[Category: ~~dna~~ architectural protein]]	+	[[Category: Dna architectural protein]]
-	[[Category: ~~phage~~ excision]]	+	[[Category: Phage excision]]
-	[[Category: ~~protein~~-dna complex]]	+	[[Category: Protein-dna complex]]
-	[[Category: ~~site~~-specific recombination recombination]]	+	[[Category: Site-specific recombination recombination]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:49:41 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 04~~:17:15 2008''	+