2pfi - Revision history

OCA at 09:09, 21 February 2024

2024-02-21T09:09:18Z

←Older revision		Revision as of 09:09, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='2pfi' size='340' side='right'caption='[[2pfi]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2pfi' size='340' side='right'caption='[[2pfi]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2pfi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PFI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2pfi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PFI FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~, ~~<scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~CLCNKA ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfi OCA], [https://pdbe.org/2pfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pfi RCSB], [https://www.ebi.ac.uk/pdbsum/2pfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pfi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfi OCA], [https://pdbe.org/2pfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pfi RCSB], [https://www.ebi.ac.uk/pdbsum/2pfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pfi ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Defects in CLCNKA are a cause of Bartter syndrome type 4B (BS4B) [MIM:[https://omim.org/entry/613090 613090]]. A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness.<ref>PMID:18310267</ref> <ref>PMID:15044642</ref>	+	[https://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN] Defects in CLCNKA are a cause of Bartter syndrome type 4B (BS4B) [MIM:[https://omim.org/entry/613090 613090]. A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness.<ref>PMID:18310267</ref> <ref>PMID:15044642</ref>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.	+	[https://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN] Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pfi ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pfi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The cytoplasmic domains of ClC chloride channels and transporters are ubiquitously found in eukaryotic family members and have been suggested to be involved in the regulation of ion transport. All cytoplasmic ClC domains share a conserved scaffold that contains a pair of CBS motifs. Here we describe the structure of the cytoplasmic component of the human chloride channel ClC-Ka at 1.6 A resolution. The structure reveals a dimeric organization of the domain that is unusual for CBS motif containing proteins. Using a biochemical approach combining mutagenesis, crosslinking, and analytical ultracentrifugation, we demonstrate that the interaction interface is preserved in solution and that the distantly related channel ClC-0 likely exhibits a similar structural organization. Our results reveal a conserved interaction interface that relates the cytoplasmic domains of ClC proteins and establish a structural relationship that is likely general for this important family of transport proteins.
-
-	The structure of the cytoplasmic domain of the chloride channel ClC-Ka reveals a conserved interaction interface.,Markovic S, Dutzler R Structure. 2007 Jun;15(6):715-25. PMID:17562318<ref>PMID:17562318</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2pfi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 38:		Line 29:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dutzler, R]]	+	[[Category: Dutzler R]]
-	[[Category: Markovic, S]]	+	[[Category: Markovic S]]
-	~~[[Category: Transport protein~~]]	+

OCA at 15:22, 17 June 2021

2021-06-17T15:22:47Z

←Older revision		Revision as of 15:22, 17 June 2021
Line 1:		Line 1:

	==Crystal structure of the cytoplasmic domain of the human chloride channel ClC-Ka==		==Crystal structure of the cytoplasmic domain of the human chloride channel ClC-Ka==
-	<StructureSection load='2pfi' size='340' side='right' caption='[[2pfi]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='2pfi' size='340' side='right'caption='[[2pfi]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2pfi]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2PFI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2pfi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PFI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CLCNKA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CLCNKA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfi OCA], [~~http~~://pdbe.org/2pfi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2pfi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2pfi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2pfi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfi OCA], [https://pdbe.org/2pfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pfi RCSB], [https://www.ebi.ac.uk/pdbsum/2pfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pfi ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Defects in CLCNKA are a cause of Bartter syndrome type 4B (BS4B) [MIM:[~~http~~://omim.org/entry/613090 613090]]. A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness.<ref>PMID:18310267</ref> <ref>PMID:15044642</ref>	+	[[https://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Defects in CLCNKA are a cause of Bartter syndrome type 4B (BS4B) [MIM:[https://omim.org/entry/613090 613090]]. A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness.<ref>PMID:18310267</ref> <ref>PMID:15044642</ref>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.	+	[[https://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:

	==See Also==		==See Also==
-	*[[Ion channels\|Ion channels]]	+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 39:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Dutzler, R]]		[[Category: Dutzler, R]]
	[[Category: Markovic, S]]		[[Category: Markovic, S]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 08:27, 29 August 2018

2018-08-29T08:27:26Z

←Older revision		Revision as of 08:27, 29 August 2018
Line 1:		Line 1:
		+
	==Crystal structure of the cytoplasmic domain of the human chloride channel ClC-Ka==		==Crystal structure of the cytoplasmic domain of the human chloride channel ClC-Ka==
	<StructureSection load='2pfi' size='340' side='right' caption='[[2pfi]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2pfi' size='340' side='right' caption='[[2pfi]], [[Resolution\|resolution]] 1.60Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CLCNKA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CLCNKA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfi OCA], [http://pdbe.org/2pfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pfi RCSB], [http://www.ebi.ac.uk/pdbsum/2pfi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfi OCA], [http://pdbe.org/2pfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pfi RCSB], [http://www.ebi.ac.uk/pdbsum/2pfi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pfi ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pf/2pfi_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pf/2pfi_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 06:07, 10 February 2016

2016-02-10T06:07:15Z

←Older revision		Revision as of 06:07, 10 February 2016
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pfi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:06, 11 September 2015

2015-09-11T06:06:58Z

←Older revision		Revision as of 06:06, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2pfi' size='340' side='right' caption='[[2pfi]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2pfi' size='340' side='right' caption='[[2pfi]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2pfi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PFI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2pfi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PFI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CLCNKA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CLCNKA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pfi RCSB], [http://www.ebi.ac.uk/pdbsum/2pfi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfi OCA], [http://pdbe.org/2pfi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pfi RCSB], [http://www.ebi.ac.uk/pdbsum/2pfi PDBsum]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2pfi" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ion channels\|Ion channels]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Dutzler, R]]		[[Category: Dutzler, R]]
	[[Category: Markovic, S]]		[[Category: Markovic, S]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 16:32, 19 January 2015

2015-01-19T16:32:08Z

←Older revision		Revision as of 16:32, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pfi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PFI FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pfi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PFI FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CLCNKA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CLCNKA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pfi RCSB], [http://www.ebi.ac.uk/pdbsum/2pfi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pfi RCSB], [http://www.ebi.ac.uk/pdbsum/2pfi PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Disease ==		== Disease ==
	[[http://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Defects in CLCNKA are a cause of Bartter syndrome type 4B (BS4B) [MIM:[http://omim.org/entry/613090 613090]]. A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness.<ref>PMID:18310267</ref> <ref>PMID:15044642</ref>		[[http://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Defects in CLCNKA are a cause of Bartter syndrome type 4B (BS4B) [MIM:[http://omim.org/entry/613090 613090]]. A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness.<ref>PMID:18310267</ref> <ref>PMID:15044642</ref>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Dutzler, R.]]	+	[[Category: Dutzler, R]]
-	[[Category: Markovic, S.]]	+	[[Category: Markovic, S]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 19:19, 30 September 2014

2014-09-30T19:19:13Z

←Older revision		Revision as of 19:19, 30 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2pfi\| PDB=2pfi \| SCENE= }}~~	+	==Crystal structure of the cytoplasmic domain of the human chloride channel ClC-Ka==
-	===Crystal structure of the cytoplasmic domain of the human chloride channel ClC-Ka===	+	<StructureSection load='2pfi' size='340' side='right' caption='[[2pfi]], [[Resolution\|resolution]] 1.60Å' scene=''>
-	~~{{ABSTRACT_PUBMED_17562318}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[2pfi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PFI FirstGlance]. <br>
-	==Disease==	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene><br>
-	[[http://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Defects in CLCNKA are a cause of Bartter syndrome type 4B (BS4B) [MIM:[http://omim.org/entry/613090 613090]]. A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness.<ref>PMID:18310267</ref><ref>PMID:15044642</ref>	+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CLCNKA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pfi RCSB], [http://www.ebi.ac.uk/pdbsum/2pfi PDBsum]</span></td></tr>
-	==Function==	+	<table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Defects in CLCNKA are a cause of Bartter syndrome type 4B (BS4B) [MIM:[http://omim.org/entry/613090 613090]]. A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness.<ref>PMID:18310267</ref> <ref>PMID:15044642</ref>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.		[[http://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pf/2pfi_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cytoplasmic domains of ClC chloride channels and transporters are ubiquitously found in eukaryotic family members and have been suggested to be involved in the regulation of ion transport. All cytoplasmic ClC domains share a conserved scaffold that contains a pair of CBS motifs. Here we describe the structure of the cytoplasmic component of the human chloride channel ClC-Ka at 1.6 A resolution. The structure reveals a dimeric organization of the domain that is unusual for CBS motif containing proteins. Using a biochemical approach combining mutagenesis, crosslinking, and analytical ultracentrifugation, we demonstrate that the interaction interface is preserved in solution and that the distantly related channel ClC-0 likely exhibits a similar structural organization. Our results reveal a conserved interaction interface that relates the cytoplasmic domains of ClC proteins and establish a structural relationship that is likely general for this important family of transport proteins.

-	~~==About this Structure==~~	+	The structure of the cytoplasmic domain of the chloride channel ClC-Ka reveals a conserved interaction interface.,Markovic S, Dutzler R Structure. 2007 Jun;15(6):715-25. PMID:17562318<ref>PMID:17562318</ref>
-	~~[[2pfi]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2PFI OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:017562318</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Dutzler, R.]]		[[Category: Dutzler, R.]]
	[[Category: Markovic, S.]]		[[Category: Markovic, S.]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 01:15, 25 March 2013

2013-03-25T01:15:05Z

←Older revision		Revision as of 01:15, 25 March 2013
Line 1:		Line 1:
-	[[Image:2pfi.png\|left\|200px]]
-
	{{STRUCTURE_2pfi\| PDB=2pfi \| SCENE= }}		{{STRUCTURE_2pfi\| PDB=2pfi \| SCENE= }}
-
	===Crystal structure of the cytoplasmic domain of the human chloride channel ClC-Ka===		===Crystal structure of the cytoplasmic domain of the human chloride channel ClC-Ka===
		+	{{ABSTRACT_PUBMED_17562318}}

-	~~{{ABSTRACT_PUBMED_17562318}}~~	+	==Disease==
		+	[[http://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Defects in CLCNKA are a cause of Bartter syndrome type 4B (BS4B) [MIM:[http://omim.org/entry/613090 613090]]. A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness.<ref>PMID:18310267</ref><ref>PMID:15044642</ref>
		+
		+	==Function==
		+	[[http://www.uniprot.org/uniprot/CLCKA_HUMAN CLCKA_HUMAN]] Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.

	==About this Structure==		==About this Structure==
Line 11:		Line 13:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:017562318</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017562318</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Dutzler, R.]]		[[Category: Dutzler, R.]]
	[[Category: Markovic, S.]]		[[Category: Markovic, S.]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA: Protected "2pfi" [edit=sysop:move=sysop]

2013-01-07T11:38:55Z

Protected "2pfi" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:38, 7 January 2013

OCA at 11:38, 7 January 2013

2013-01-07T11:38:54Z

←Older revision		Revision as of 11:38, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2pfi.png\|left\|200px]]		[[Image:2pfi.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2pfi", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2pfi\| PDB=2pfi \| SCENE= }}		{{STRUCTURE_2pfi\| PDB=2pfi \| SCENE= }}

	===Crystal structure of the cytoplasmic domain of the human chloride channel ClC-Ka===		===Crystal structure of the cytoplasmic domain of the human chloride channel ClC-Ka===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17562318}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17562318 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17562318}}		{{ABSTRACT_PUBMED_17562318}}
-
-	==Disease==
-	Known disease associated with this structure: Bartter syndrome, type 4, digenic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=602024 602024]]

	==About this Structure==		==About this Structure==
-	~~2PFI~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFI OCA].	+	[[2pfi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFI OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17562318~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017562318</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Dutzler, R.]]		[[Category: Dutzler, R.]]
	[[Category: Markovic, S.]]		[[Category: Markovic, S.]]
-		+	[[Category: Transport protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 16:01:05 2009''~~	+