2psh - Revision history

OCA at 09:11, 21 February 2024

2024-02-21T09:11:45Z

←Older revision		Revision as of 09:11, 21 February 2024
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	<StructureSection load='2psh' size='340' side='right'caption='[[2psh]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='2psh' size='340' side='right'caption='[[2psh]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2psh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Pennatula_reniformis Pennatula~~ reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PSH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2psh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PSH FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2psd\|2psd]], [[2pse\|2pse]], [[2psf\|2psf]], [[2psj\|2psj]], [[2psl\|2psl]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.79Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">rluc ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=6136 Pennatula reniformis])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psh OCA], [https://pdbe.org/2psh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2psh RCSB], [https://www.ebi.ac.uk/pdbsum/2psh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2psh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psh OCA], [https://pdbe.org/2psh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2psh RCSB], [https://www.ebi.ac.uk/pdbsum/2psh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2psh ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LUCI_RENRE LUCI_RENRE]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2psh ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2psh ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely employed in molecular biology as a reporter gene in cell culture experiments and small animal imaging. To accomplish this bioluminescence, the 37-kDa enzyme catalyzes the degradation of its substrate coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light. We successfully crystallized a stabilized variant of this important protein (RLuc8) and herein present the first structures for any coelenterazine-using luciferase. These structures are based on high-resolution data measured to 1.4 A and demonstrate a classic alpha/beta-hydrolase fold. We also present data of a coelenteramide-bound luciferase and reason that this structure represents a secondary conformational form following shift of the product out of the primary active site. During the course of this work, the structure of the luciferase's accessory green fluorescent protein (RrGFP) was also determined and shown to be highly similar to that of Aequorea victoria GFP.
-
-	Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis.,Loening AM, Fenn TD, Gambhir SS J Mol Biol. 2007 Dec 7;374(4):1017-28. Epub 2007 Oct 3. PMID:17980388<ref>PMID:17980388</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2psh" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Luciferase 3D structures\|Luciferase 3D structures]]		*[[Luciferase 3D structures\|Luciferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pennatula~~ reniformis]]	+	[[Category: Renilla reniformis]]
-	~~[[Category: Renilla-luciferin 2-monooxygenase~~]]	+	[[Category: Fenn TD]]
-	[[Category: Fenn~~, T D~~]]	+	[[Category: Gambhir SS]]
-	[[Category: Gambhir~~, S S~~]]	+	[[Category: Loening AM]]
-	[[Category: Loening~~, A M]]~~	+
-	~~[[Category: Alpha/beta-hydrolase]]~~	+
-	~~[[Category: Luciferase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 18:28, 20 October 2021

2021-10-20T18:28:50Z

←Older revision		Revision as of 18:28, 20 October 2021
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	<StructureSection load='2psh' size='340' side='right'caption='[[2psh]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='2psh' size='340' side='right'caption='[[2psh]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2psh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Renre Renre~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PSH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2psh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pennatula_reniformis Pennatula reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PSH FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2psd\|2psd]], [[2pse\|2pse]], [[2psf\|2psf]], [[2psj\|2psj]], [[2psl\|2psl]]</div></td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2psd\|2psd]], [[2pse\|2pse]], [[2psf\|2psf]], [[2psj\|2psj]], [[2psl\|2psl]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 ~~RENRE~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 Pennatula reniformis])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psh OCA], [https://pdbe.org/2psh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2psh RCSB], [https://www.ebi.ac.uk/pdbsum/2psh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2psh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psh OCA], [https://pdbe.org/2psh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2psh RCSB], [https://www.ebi.ac.uk/pdbsum/2psh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2psh ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Pennatula reniformis]]
	[[Category: Renilla-luciferin 2-monooxygenase]]		[[Category: Renilla-luciferin 2-monooxygenase]]
-	[[Category: Renre]]
	[[Category: Fenn, T D]]		[[Category: Fenn, T D]]
	[[Category: Gambhir, S S]]		[[Category: Gambhir, S S]]

OCA at 15:31, 17 June 2021

2021-06-17T15:31:52Z

←Older revision		Revision as of 15:31, 17 June 2021
Line 1:		Line 1:

	==Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis==		==Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis==
-	<StructureSection load='2psh' size='340' side='right' caption='[[2psh]], [[Resolution\|resolution]] 1.79Å' scene=''>	+	<StructureSection load='2psh' size='340' side='right'caption='[[2psh]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2psh]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Renre Renre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2PSH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2psh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Renre Renre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PSH FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2psd\|2psd]], [[2pse\|2pse]], [[2psf\|2psf]], [[2psj\|2psj]], [[2psl\|2psl]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2psd\|2psd]], [[2pse\|2pse]], [[2psf\|2psf]], [[2psj\|2psj]], [[2psl\|2psl]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 RENRE])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 RENRE])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psh OCA], [~~http~~://pdbe.org/2psh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2psh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2psh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2psh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psh OCA], [https://pdbe.org/2psh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2psh RCSB], [https://www.ebi.ac.uk/pdbsum/2psh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2psh ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
-	*[[Luciferase\|Luciferase]]	+	*[[Luciferase 3D structures\|Luciferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Renilla-luciferin 2-monooxygenase]]		[[Category: Renilla-luciferin 2-monooxygenase]]
	[[Category: Renre]]		[[Category: Renre]]

OCA at 08:27, 29 August 2018

2018-08-29T08:27:23Z

←Older revision		Revision as of 08:27, 29 August 2018
Line 1:		Line 1:
		+
	==Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis==		==Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis==
	<StructureSection load='2psh' size='340' side='right' caption='[[2psh]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='2psh' size='340' side='right' caption='[[2psh]], [[Resolution\|resolution]] 1.79Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 RENRE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 RENRE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psh OCA], [http://pdbe.org/2psh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2psh RCSB], [http://www.ebi.ac.uk/pdbsum/2psh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psh OCA], [http://pdbe.org/2psh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2psh RCSB], [http://www.ebi.ac.uk/pdbsum/2psh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2psh ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/2psh_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/2psh_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2psh ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:39, 11 September 2015

2015-09-11T13:39:53Z

←Older revision		Revision as of 13:39, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2psh' size='340' side='right' caption='[[2psh]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='2psh' size='340' side='right' caption='[[2psh]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2psh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Renilla_reniformis Renilla reniformis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2psh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Renre Renre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSH FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2psd\|2psd]], [[2pse\|2pse]], [[2psf\|2psf]], [[2psj\|2psj]], [[2psl\|2psl]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2psd\|2psd]], [[2pse\|2pse]], [[2psf\|2psf]], [[2psj\|2psj]], [[2psl\|2psl]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 ~~Renilla reniformis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 RENRE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2psh RCSB], [http://www.ebi.ac.uk/pdbsum/2psh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psh OCA], [http://pdbe.org/2psh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2psh RCSB], [http://www.ebi.ac.uk/pdbsum/2psh PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2psh" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Renilla reniformis]]
	[[Category: Renilla-luciferin 2-monooxygenase]]		[[Category: Renilla-luciferin 2-monooxygenase]]
		+	[[Category: Renre]]
	[[Category: Fenn, T D]]		[[Category: Fenn, T D]]
	[[Category: Gambhir, S S]]		[[Category: Gambhir, S S]]

OCA at 13:31, 19 January 2015

2015-01-19T13:31:24Z

←Older revision		Revision as of 13:31, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2psh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2psh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSH FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2psd\|2psd]], [[2pse\|2pse]], [[2psf\|2psf]], [[2psj\|2psj]], [[2psl\|2psl]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2psd\|2psd]], [[2pse\|2pse]], [[2psf\|2psf]], [[2psj\|2psj]], [[2psl\|2psl]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 Renilla reniformis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 Renilla reniformis])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2psh RCSB], [http://www.ebi.ac.uk/pdbsum/2psh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2psh RCSB], [http://www.ebi.ac.uk/pdbsum/2psh PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Renilla reniformis]]		[[Category: Renilla reniformis]]
	[[Category: Renilla-luciferin 2-monooxygenase]]		[[Category: Renilla-luciferin 2-monooxygenase]]
-	[[Category: Fenn, T D.]]	+	[[Category: Fenn, T D]]
-	[[Category: Gambhir, S S.]]	+	[[Category: Gambhir, S S]]
-	[[Category: Loening, A M.]]	+	[[Category: Loening, A M]]
	[[Category: Alpha/beta-hydrolase]]		[[Category: Alpha/beta-hydrolase]]
	[[Category: Luciferase]]		[[Category: Luciferase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 06:32, 29 September 2014

2014-09-29T06:32:23Z

←Older revision		Revision as of 06:32, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2psh.~~png~~\|~~left~~\|200px]]	+	==Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis==
		+	<StructureSection load='2psh' size='340' side='right' caption='[[2psh]], [[Resolution\|resolution]] 1.79Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2psh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PSH FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2psd\|2psd]], [[2pse\|2pse]], [[2psf\|2psf]], [[2psj\|2psj]], [[2psl\|2psl]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rluc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6136 Renilla reniformis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Renilla-luciferin_2-monooxygenase Renilla-luciferin 2-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.5 1.13.12.5] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2psh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2psh RCSB], [http://www.ebi.ac.uk/pdbsum/2psh PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/2psh_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely employed in molecular biology as a reporter gene in cell culture experiments and small animal imaging. To accomplish this bioluminescence, the 37-kDa enzyme catalyzes the degradation of its substrate coelenterazine in the presence of molecular oxygen, resulting in the product coelenteramide, carbon dioxide, and the desired photon of light. We successfully crystallized a stabilized variant of this important protein (RLuc8) and herein present the first structures for any coelenterazine-using luciferase. These structures are based on high-resolution data measured to 1.4 A and demonstrate a classic alpha/beta-hydrolase fold. We also present data of a coelenteramide-bound luciferase and reason that this structure represents a secondary conformational form following shift of the product out of the primary active site. During the course of this work, the structure of the luciferase's accessory green fluorescent protein (RrGFP) was also determined and shown to be highly similar to that of Aequorea victoria GFP.

-	~~{{STRUCTURE_2psh\| PDB=2psh \| SCENE= }}~~	+	Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis.,Loening AM, Fenn TD, Gambhir SS J Mol Biol. 2007 Dec 7;374(4):1017-28. Epub 2007 Oct 3. PMID:17980388<ref>PMID:17980388</ref>

-	~~===Crystal Structures~~ of the ~~Luciferase and Green Fluorescent Protein from Renilla Reniformis===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17980388}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2psh]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Renilla_reniformis Renilla reniformis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA].~~	+

	==See Also==		==See Also==
	*[[Luciferase\|Luciferase]]		*[[Luciferase\|Luciferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:017980388<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Renilla reniformis]]		[[Category: Renilla reniformis]]
	[[Category: Renilla-luciferin 2-monooxygenase]]		[[Category: Renilla-luciferin 2-monooxygenase]]

OCA at 19:18, 20 October 2012

2012-10-20T19:18:59Z

←Older revision		Revision as of 19:18, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2psh]] is a 2 chain structure ~~of [[Luciferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA].	+	[[2psh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA].

	==See Also==		==See Also==

OCA: Protected "2psh" [edit=sysop:move=sysop]

2012-07-27T06:57:10Z

Protected "2psh" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:57, 27 July 2012

OCA at 06:57, 27 July 2012

2012-07-27T06:57:08Z

←Older revision		Revision as of 06:57, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2psh.png\|left\|200px]]		[[Image:2psh.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2psh", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2psh\| PDB=2psh \| SCENE= }}		{{STRUCTURE_2psh\| PDB=2psh \| SCENE= }}

	===Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis===		===Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17980388}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17980388 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17980388}}		{{ABSTRACT_PUBMED_17980388}}

	==About this Structure==		==About this Structure==
-	~~2PSH~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA].	+	[[2psh]] is a 2 chain structure of [[Luciferase]] with sequence from [http://en.wikipedia.org/wiki/Renilla_reniformis Renilla reniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PSH OCA].
		+
		+	==See Also==
		+	*[[Luciferase\|Luciferase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17980388~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017980388</ref><references group="xtra"/>
	[[Category: Renilla reniformis]]		[[Category: Renilla reniformis]]
	[[Category: Renilla-luciferin 2-monooxygenase]]		[[Category: Renilla-luciferin 2-monooxygenase]]
Line 32:		Line 23:
	[[Category: Luciferase]]		[[Category: Luciferase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 23:50:17 2009''