2qou - Revision history

OCA: Redirecting to 4v56

2014-12-10T15:13:16Z

Redirecting to 4v56

←Older revision		Revision as of 15:13, 10 December 2014
Line 1:		Line 1:
-	==Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the first 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.==	+	#REDIRECT [[4v56]] This PDB entry is obsolete and replaced by 4v56
-	~~<StructureSection load='2qou' size='340' side='right' caption='~~[[~~2qou]], [[Resolution\|resolution]] 3.93Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[2qou~~]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QOU FirstGlance]. <br>	+
-	~~</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SCM:SPECTINOMYCIN'>SCM</scene><br>~~	+
-	~~<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qov\|2qov]], [[2qow\|2qow]], [[2qox\|2qox]]</td></tr>~~	+
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qou OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qou RCSB], [http://www.ebi.ac.uk/pdbsum/2qou PDBsum]</span></td></tr>	+
-	~~<table>~~	+
-	~~== Evolutionary Conservation ==~~	+
-	~~[[Image:Consurf_key_small.gif\|200px\|right]]~~	+
-	~~Check<jmol>~~	+
-	~~<jmolCheckbox>~~	+
-	~~<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qo/2qou_consurf.spt"</scriptWhenChecked>~~	+
-	~~<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>~~	+
-	~~<text>to colour the structure by Evolutionary Conservation</text>~~	+
-	~~</jmolCheckbox>~~	+
-	~~</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method~~ and ~~the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].~~	+
-	~~<div style="clear:both"></div>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	~~The widely used antibiotic spectinomycin inhibits bacterial protein synthesis~~ by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle.	+
-		+
-	A steric block in translation caused by the antibiotic spectinomycin.,Borovinskaya MA, Shoji S, Holton JM, Fredrick K, Cate JH ACS Chem Biol. 2007 Aug 17;2(8):545-52. Epub 2007 Aug 10. PMID:17696316<ref>PMID:17696316</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-		+
-	~~==See Also==~~	+
-	*[[Ribosome 3D structures\|Ribosome 3D structures]]	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Escherichia coli]]~~	+
-	~~[[Category: Borovinskaya, M A.]]~~	+
-	~~[[Category: Cate, J H.D.]]~~	+
-	~~[[Category: Fredrick, K.]]~~	+
-	~~[[Category: Holton, J M.]]~~	+
-	~~[[Category: Shoji, S.]]~~	+
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Endonuclease]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Methylation]]~~	+
-	~~[[Category: Nuclease]]~~	+
-	~~[[Category: Repressor]]~~	+
-	~~[[Category: Ribonucleoprotein]]~~	+
-	~~[[Category: Ribosomal protein]]~~	+
-	~~[[Category: Ribosome]]~~	+
-	~~[[Category: Rna-binding]]~~	+
-	~~[[Category: Rna-protein complex]]~~	+
-	~~[[Category: Rrna-binding]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription regulation]]~~	+
-	~~[[Category: Transcription termination]]~~	+
-	~~[[Category: Translation regulation]]~~	+
-	~~[[Category: Trna-binding]]~~	+

OCA at 09:13, 29 September 2014

2014-09-29T09:13:29Z

←Older revision		Revision as of 09:13, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2qou.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the first 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.==
		+	<StructureSection load='2qou' size='340' side='right' caption='[[2qou]], [[Resolution\|resolution]] 3.93Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2qou]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QOU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SCM:SPECTINOMYCIN'>SCM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qov\|2qov]], [[2qow\|2qow]], [[2qox\|2qox]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qou OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qou RCSB], [http://www.ebi.ac.uk/pdbsum/2qou PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qo/2qou_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle.

-	~~{{STRUCTURE_2qou\| PDB=2qou \| SCENE= }}~~	+	A steric block in translation caused by the antibiotic spectinomycin.,Borovinskaya MA, Shoji S, Holton JM, Fredrick K, Cate JH ACS Chem Biol. 2007 Aug 17;2(8):545-52. Epub 2007 Aug 10. PMID:17696316<ref>PMID:17696316</ref>

-	~~===Crystal structure~~ of the ~~bacterial ribosome from Escherichia coli in complex with spectinomycin~~. ~~This file contains the 30S subunit~~ of ~~the first 70S ribosome, with spectinomycin bound~~. ~~The entire crystal structure contains two 70S ribosomes.===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17696316}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2qou]] is a 21 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOU OCA].~~	+

	==See Also==		==See Also==
-	*[[~~Ribosomal protein S10~~\|~~Ribosomal protein S10~~]]	+	*[[Ribosome 3D structures\|Ribosome 3D structures]]
-	*[[Ribosomal protein S11\|Ribosomal protein S11]]	+	== References ==
-	*[[Ribosomal protein S12\|Ribosomal protein S12]]	+	<references/>
-	*[[Ribosomal protein S13\|Ribosomal protein S13]]	+	__TOC__
-	*[[Ribosomal protein S14\|Ribosomal protein S14]]	+	</StructureSection>
-	*[[Ribosomal protein S15\|Ribosomal protein S15]]	+
-	*[[Ribosomal protein S16\|Ribosomal protein S16]]	+
-	*[[Ribosomal protein S17\|Ribosomal protein S17]]	+
-	*[[Ribosomal protein S18\|Ribosomal protein S18]]	+
-	*[[Ribosomal protein S19\|Ribosomal protein S19]]	+
-	*[[Ribosomal protein S20\|Ribosomal protein S20]]	+
-	*[[Ribosomal protein S21\|Ribosomal protein S21]]	+
-	*[[Ribosomal protein S3\|Ribosomal protein S3]]	+
-	*[[Ribosomal protein S4\|Ribosomal protein S4]]	+
-	*[[Ribosomal protein S5\|Ribosomal protein S5]]	+
-	*[[Ribosomal protein S6\|Ribosomal protein S6]]	+
-	*[[Ribosomal protein S7\|Ribosomal protein S7]]	+
-	*[[Ribosomal protein S8\|Ribosomal protein S8]]	+
-	*[[Ribosomal protein S9\|Ribosomal protein S9]]	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017696316<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Borovinskaya, M A.]]		[[Category: Borovinskaya, M A.]]

OCA at 10:20, 20 October 2012

2012-10-20T10:20:47Z

←Older revision		Revision as of 10:20, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2qou]] is a 21 chain structure of [[Ribosomal protein S10]], [[Ribosomal protein S11]], [[Ribosomal protein S12]], [[Ribosomal protein S13]], [[Ribosomal protein S14]], [[Ribosomal protein S15]], [[Ribosomal protein S16]], [[Ribosomal protein S17]], [[Ribosomal protein S18]], [[Ribosomal protein S19]], [[Ribosomal protein S20]], [[Ribosomal protein S21]], [[Ribosomal protein S3]], [[Ribosomal protein S4]], [[Ribosomal protein S5]], [[Ribosomal protein S6]], [[Ribosomal protein S7]], [[Ribosomal protein S8]] and [[Ribosomal protein S9]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOU OCA].	+	[[2qou]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOU OCA].

	==See Also==		==See Also==

OCA at 01:49, 26 July 2012

2012-07-26T01:49:42Z

←Older revision		Revision as of 01:49, 26 July 2012
Line 1:		Line 1:
	[[Image:2qou.png\|left\|200px]]		[[Image:2qou.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2qou", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2qou\| PDB=2qou \| SCENE= }}		{{STRUCTURE_2qou\| PDB=2qou \| SCENE= }}

	===Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the first 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.===		===Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the first 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17696316}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17696316 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17696316}}		{{ABSTRACT_PUBMED_17696316}}

	==About this Structure==		==About this Structure==
-	[[2qou]] is a 21 chain structure of [[Ribosomal protein S10]], [[Ribosomal protein S11]], [[Ribosomal protein S12]], [[Ribosomal protein S13]], [[Ribosomal protein S15]], [[Ribosomal protein S17]], [[Ribosomal protein S2]], [[Ribosomal protein S20]], [[Ribosomal protein S21]], [[Ribosomal protein S3]] ~~and~~ [[Ribosomal protein S4]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOU OCA].	+	[[2qou]] is a 21 chain structure of [[Ribosomal protein S10]], [[Ribosomal protein S11]], [[Ribosomal protein S12]], [[Ribosomal protein S13]], [[Ribosomal protein S14]], [[Ribosomal protein S15]], [[Ribosomal protein S16]], [[Ribosomal protein S17]], [[Ribosomal protein S18]], [[Ribosomal protein S19]], [[Ribosomal protein S20]], [[Ribosomal protein S21]], [[Ribosomal protein S3]], [[Ribosomal protein S4]], [[Ribosomal protein S5]], [[Ribosomal protein S6]], [[Ribosomal protein S7]], [[Ribosomal protein S8]] and [[Ribosomal protein S9]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOU OCA].

	==See Also==		==See Also==
-	*[[Ribosomal protein S10]]	+	*[[Ribosomal protein S10\|Ribosomal protein S10]]
-	*[[Ribosomal protein S11]]	+	*[[Ribosomal protein S11\|Ribosomal protein S11]]
-	*[[Ribosomal protein S12]]	+	*[[Ribosomal protein S12\|Ribosomal protein S12]]
-	*[[Ribosomal protein S13]]	+	*[[Ribosomal protein S13\|Ribosomal protein S13]]
-	*[[Ribosomal protein S15]]	+	*[[Ribosomal protein S14\|Ribosomal protein S14]]
-	*[[Ribosomal protein S17]]	+	*[[Ribosomal protein S15\|Ribosomal protein S15]]
-	*[[Ribosomal protein S2]]	+	*[[Ribosomal protein S16\|Ribosomal protein S16]]
-	*[[Ribosomal protein S20]]	+	*[[Ribosomal protein S17\|Ribosomal protein S17]]
-	*[[Ribosomal protein S21]]	+	*[[Ribosomal protein S18\|Ribosomal protein S18]]
-	*[[Ribosomal protein S3]]	+	*[[Ribosomal protein S19\|Ribosomal protein S19]]
-	*[[Ribosomal protein S4]]	+	*[[Ribosomal protein S20\|Ribosomal protein S20]]
		+	*[[Ribosomal protein S21\|Ribosomal protein S21]]
		+	*[[Ribosomal protein S3\|Ribosomal protein S3]]
		+	*[[Ribosomal protein S4\|Ribosomal protein S4]]
		+	*[[Ribosomal protein S5\|Ribosomal protein S5]]
		+	*[[Ribosomal protein S6\|Ribosomal protein S6]]
		+	*[[Ribosomal protein S7\|Ribosomal protein S7]]
		+	*[[Ribosomal protein S8\|Ribosomal protein S8]]
		+	*[[Ribosomal protein S9\|Ribosomal protein S9]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17696316~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017696316</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Borovinskaya, M A.]]		[[Category: Borovinskaya, M A.]]
Line 42:		Line 39:
	[[Category: Holton, J M.]]		[[Category: Holton, J M.]]
	[[Category: Shoji, S.]]		[[Category: Shoji, S.]]
-	[[Category: Acetylation]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Endonuclease]]		[[Category: Endonuclease]]

OCA: Protected "2qou" [edit=sysop:move=sysop]

2010-12-26T21:56:29Z

Protected "2qou" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:56, 26 December 2010

OCA at 21:56, 26 December 2010

2010-12-26T21:56:27Z

←Older revision		Revision as of 21:56, 26 December 2010
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2qou.png\|left\|200px]]		[[Image:2qou.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~2QOU~~ is a 21 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOU OCA].	+	[[2qou]] is a 21 chain structure of [[Ribosomal protein S10]], [[Ribosomal protein S11]], [[Ribosomal protein S12]], [[Ribosomal protein S13]], [[Ribosomal protein S15]], [[Ribosomal protein S17]], [[Ribosomal protein S2]], [[Ribosomal protein S20]], [[Ribosomal protein S21]], [[Ribosomal protein S3]] and [[Ribosomal protein S4]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOU OCA].
		+
		+	==See Also==
		+	*[[Ribosomal protein S10]]
		+	*[[Ribosomal protein S11]]
		+	*[[Ribosomal protein S12]]
		+	*[[Ribosomal protein S13]]
		+	*[[Ribosomal protein S15]]
		+	*[[Ribosomal protein S17]]
		+	*[[Ribosomal protein S2]]
		+	*[[Ribosomal protein S20]]
		+	*[[Ribosomal protein S21]]
		+	*[[Ribosomal protein S3]]
		+	*[[Ribosomal protein S4]]

	==Reference==		==Reference==
Line 48:		Line 60:
	[[Category: Translation regulation]]		[[Category: Translation regulation]]
	[[Category: Trna-binding]]		[[Category: Trna-binding]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:54:22 2009''

OCA at 20:54, 16 February 2009

2009-02-16T20:54:27Z

←Older revision		Revision as of 20:54, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2QOU is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOU OCA].	+	2QOU is a 21 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOU OCA].

	==Reference==		==Reference==
-	~~A steric block in translation caused by the antibiotic spectinomycin., Borovinskaya MA, Shoji S, Holton JM, Fredrick K, Cate JH, ACS Chem Biol. 2007 Aug 17;2(8):545-52. Epub 2007 Aug 10.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17696316 17696316]~~	+	<ref group="xtra">PMID:17696316</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Protein complex]]
	[[Category: Borovinskaya, M A.]]		[[Category: Borovinskaya, M A.]]
	[[Category: Cate, J H.D.]]		[[Category: Cate, J H.D.]]
Line 50:		Line 49:
	[[Category: Trna-binding]]		[[Category: Trna-binding]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon ~~Jul 28 03~~:34:~~27 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:54:22 2009''

OCA at 00:34, 28 July 2008

2008-07-28T00:34:31Z

←Older revision		Revision as of 00:34, 28 July 2008
Line 1:		Line 1:
-	[[Image:2qou.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2qou.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2qou\| PDB=2qou \| SCENE= }}		{{STRUCTURE_2qou\| PDB=2qou \| SCENE= }}

-	~~'''~~Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the first 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.~~'''~~	+	===Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the first 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.===


-	~~==Overview==~~	+	<!--
-	The ~~widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here~~, ~~we show that in crystals of~~ the ~~Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of~~ the ~~head domain of the small ribosomal subunit~~. ~~Spectinomycin also alters the rate and completeness of reverse translocation in vitro~~. ~~These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of~~ the ~~head domain of the small ribosomal subunit and thereby disrupts the translocation cycle~~.	+	The line below this paragraph, {{ABSTRACT_PUBMED_17696316}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17696316 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_17696316}}

	==About this Structure==		==About this Structure==
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	[[Category: Translation regulation]]		[[Category: Translation regulation]]
	[[Category: Trna-binding]]		[[Category: Trna-binding]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 15~~:20:16 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:34:27 2008''

OCA at 12:20, 4 May 2008

2008-05-04T12:20:20Z

←Older revision		Revision as of 12:20, 4 May 2008
Line 1:		Line 1:
	[[Image:2qou.gif\|left\|200px]]		[[Image:2qou.gif\|left\|200px]]

-	{{Structure	+	<!--
-	\|PDB~~= 2qou \|SIZE=350\|CAPTION= <~~scene ~~name='initialview01'>2qou</scene>~~, ~~resolution 3~~.~~93Å~~	+	The line below this paragraph, containing "STRUCTURE_2qou", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <scene name='pdbligand=A:ADENOSINE~~-~~5'~~-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SCM:SPECTINOMYCIN'>SCM</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	\|~~ACTIVITY~~=	+	or leave the SCENE parameter empty for the default display.
-	\|~~GENE~~=	+	-->
-	~~\|DOMAIN=~~	+	{{STRUCTURE_2qou\| PDB=2qou \| SCENE= }}
-	~~\|RELATEDENTRY=[[2qov\|2QOV]], [[2qow\|2QOW]], [[2qox\|2QOX]]~~	+
-	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qou OCA], [http://www.ebi.ac.uk/pdbsum/2qou PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qou RCSB]</span>	+
-	}}	+

	'''Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the first 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.'''		'''Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the first 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.'''
Line 30:		Line 27:
	[[Category: Holton, J M.]]		[[Category: Holton, J M.]]
	[[Category: Shoji, S.]]		[[Category: Shoji, S.]]
-	[[Category: ~~acetylation~~]]	+	[[Category: Acetylation]]
-	[[Category: ~~antibiotic~~ resistance]]	+	[[Category: Antibiotic resistance]]
-	[[Category: ~~endonuclease~~]]	+	[[Category: Endonuclease]]
-	[[Category: ~~hydrolase~~]]	+	[[Category: Hydrolase]]
-	[[Category: ~~methylation~~]]	+	[[Category: Methylation]]
-	[[Category: ~~nuclease~~]]	+	[[Category: Nuclease]]
-	[[Category: ~~repressor~~]]	+	[[Category: Repressor]]
-	[[Category: ~~ribonucleoprotein~~]]	+	[[Category: Ribonucleoprotein]]
-	[[Category: ~~ribosomal~~ protein]]	+	[[Category: Ribosomal protein]]
-	[[Category: ~~ribosome~~]]	+	[[Category: Ribosome]]
-	[[Category: ~~rna~~-binding]]	+	[[Category: Rna-binding]]
-	[[Category: ~~rna~~-protein complex]]	+	[[Category: Rna-protein complex]]
-	[[Category: ~~rrna~~-binding]]	+	[[Category: Rrna-binding]]
-	[[Category: ~~transcription~~]]	+	[[Category: Transcription]]
-	[[Category: ~~transcription~~ regulation]]	+	[[Category: Transcription regulation]]
-	[[Category: ~~transcription~~ termination]]	+	[[Category: Transcription termination]]
-	[[Category: ~~translation~~ regulation]]	+	[[Category: Translation regulation]]
-	[[Category: ~~trna~~-binding]]	+	[[Category: Trna-binding]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:20:16 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 04~~:51:36 2008''	+

OCA at 01:51, 31 March 2008

2008-03-31T01:51:44Z

←Older revision		Revision as of 01:51, 31 March 2008
Line 4:		Line 4:
	\|PDB= 2qou \|SIZE=350\|CAPTION= <scene name='initialview01'>2qou</scene>, resolution 3.93Å		\|PDB= 2qou \|SIZE=350\|CAPTION= <scene name='initialview01'>2qou</scene>, resolution 3.93Å
	\|SITE=		\|SITE=
-	\|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> ~~and~~ <scene name='pdbligand=SCM:SPECTINOMYCIN'>SCM</scene>	+	\|LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SCM:SPECTINOMYCIN'>SCM</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
	\|ACTIVITY=		\|ACTIVITY=
	\|GENE=		\|GENE=
		+	\|DOMAIN=
		+	\|RELATEDENTRY=[[2qov\|2QOV]], [[2qow\|2QOW]], [[2qox\|2QOX]]
		+	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qou OCA], [http://www.ebi.ac.uk/pdbsum/2qou PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qou RCSB]</span>
	}}		}}

Line 27:		Line 30:
	[[Category: Holton, J M.]]		[[Category: Holton, J M.]]
	[[Category: Shoji, S.]]		[[Category: Shoji, S.]]
-	[[Category: MG]]
-	[[Category: SCM]]
	[[Category: acetylation]]		[[Category: acetylation]]
	[[Category: antibiotic resistance]]		[[Category: antibiotic resistance]]
Line 48:		Line 49:
	[[Category: trna-binding]]		[[Category: trna-binding]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Mar ~~20 18~~:27:58 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:51:36 2008''