2qvj - Revision history

OCA at 09:18, 21 February 2024

2024-02-21T09:18:13Z

←Older revision		Revision as of 09:18, 21 February 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A6H4P1_9RHAB A6H4P1_9RHAB]		[https://www.uniprot.org/uniprot/A6H4P1_9RHAB A6H4P1_9RHAB]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structure of the vesicular stomatitis virus nucleoprotein (N) in complex with RNA reveals extensive and specific intermolecular interactions among the N molecules in the 10-member oligomer. What roles these interactions play in encapsidating RNA was studied by mutagenesis of the N protein. Three N mutants intended for disruption of the intermolecular interactions were designed and coexpressed with the phosphoprotein (P) in an Escherichia coli system previously described (T. J. Green et al., J. Virol. 74:9515-9524, 2000). Mutants N (Delta1-22), N (Delta347-352), and N (320-324, (Ala)(5)) lost RNA encapsidation and oligomerization but still bound with P. Another mutant, N (Ser290-->Trp), was able to form a stable ring-like N oligomer and bind with the P protein but was no longer able to encapsidate RNA. The crystal structure of N (Ser290-->Trp) at 2.8 A resolution showed that this mutant can maintain all the same intermolecular interactions as the wild-type N except for a slight unwinding of the N-terminal lobe. These results suggest that the intermolecular contacts among the N molecules are required for encapsidation of the viral RNA.
-
-	Role of intermolecular interactions of vesicular stomatitis virus nucleoprotein in RNA encapsidation.,Zhang X, Green TJ, Tsao J, Qiu S, Luo M J Virol. 2008 Jan;82(2):674-82. Epub 2007 Nov 14. PMID:18003727<ref>PMID:18003727</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2qvj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]		*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:10, 26 July 2023

2023-07-26T13:10:54Z

←Older revision		Revision as of 13:10, 26 July 2023
Line 3:		Line 3:
	<StructureSection load='2qvj' size='340' side='right'caption='[[2qvj]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2qvj' size='340' side='right'caption='[[2qvj]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Vsiv Vsiv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Vesicular_stomatitis_Indiana_virus Vesicular stomatitis Indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~N (~~[~~https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=11277 VSIV])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [https://pdbe.org/2qvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [https://www.ebi.ac.uk/pdbsum/2qvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qvj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [https://pdbe.org/2qvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [https://www.ebi.ac.uk/pdbsum/2qvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qvj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~NCAP_VSIVA NCAP_VSIVA~~]] Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).	+	[https://www.uniprot.org/uniprot/A6H4P1_9RHAB A6H4P1_9RHAB]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Vsiv~~]]	+	[[Category: Vesicular stomatitis Indiana virus]]
-	[[Category: Green~~, T J~~]]	+	[[Category: Green TJ]]
-	[[Category: Luo, M]]	+	[[Category: Luo M]]
-	[[Category: Qiu, S]]	+	[[Category: Qiu S]]
-	[[Category: Tsao, J]]	+	[[Category: Tsao J]]
-	[[Category: Zhang, X]]	+	[[Category: Zhang X]]
-	~~[[Category: Cytoplasm]]~~	+
-	~~[[Category: Nucleocapsid]]~~	+
-	~~[[Category: Ribonucleoprotein]]~~	+
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Rna-binding]]~~	+
-	~~[[Category: Viral nucleoprotein]]~~	+
-	~~[[Category: Virion~~]]	+

OCA at 04:07, 2 July 2021

2021-07-02T04:07:20Z

←Older revision		Revision as of 04:07, 2 July 2021
Line 1:		Line 1:

	==Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant==		==Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant==
-	<StructureSection load='2qvj' size='340' side='right' caption='[[2qvj]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='2qvj' size='340' side='right'caption='[[2qvj]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Vsiv Vsiv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Vsiv Vsiv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 VSIV])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 VSIV])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [~~http~~://pdbe.org/2qvj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2qvj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2qvj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [https://pdbe.org/2qvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [https://www.ebi.ac.uk/pdbsum/2qvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qvj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NCAP_VSIVA NCAP_VSIVA]] Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).	+	[[https://www.uniprot.org/uniprot/NCAP_VSIVA NCAP_VSIVA]] Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Nucleoprotein\|Nucleoprotein]]	+	*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Vsiv]]		[[Category: Vsiv]]
	[[Category: Green, T J]]		[[Category: Green, T J]]

OCA at 10:56, 5 September 2018

2018-09-05T10:56:44Z

←Older revision		Revision as of 10:56, 5 September 2018
Line 1:		Line 1:
		+
	==Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant==		==Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant==
	<StructureSection load='2qvj' size='340' side='right' caption='[[2qvj]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2qvj' size='340' side='right' caption='[[2qvj]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Vsiv Vsiv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Vsiv Vsiv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 VSIV])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 VSIV])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [http://pdbe.org/2qvj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [http://www.ebi.ac.uk/pdbsum/2qvj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [http://pdbe.org/2qvj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [http://www.ebi.ac.uk/pdbsum/2qvj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2qvj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 30:		Line 31:
	[[Category: Tsao, J]]		[[Category: Tsao, J]]
	[[Category: Zhang, X]]		[[Category: Zhang, X]]
		+	[[Category: Cytoplasm]]
	[[Category: Nucleocapsid]]		[[Category: Nucleocapsid]]
	[[Category: Ribonucleoprotein]]		[[Category: Ribonucleoprotein]]

OCA at 09:06, 11 September 2015

2015-09-11T09:06:56Z

←Older revision		Revision as of 09:06, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2qvj' size='340' side='right' caption='[[2qvj]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2qvj' size='340' side='right' caption='[[2qvj]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Vsiv Vsiv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 ~~Vesicular stomatitis Indiana virus~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 VSIV])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [http://www.ebi.ac.uk/pdbsum/2qvj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [http://pdbe.org/2qvj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [http://www.ebi.ac.uk/pdbsum/2qvj PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2qvj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vesicular stomatitis indiana virus~~]]	+	[[Category: Vsiv]]
	[[Category: Green, T J]]		[[Category: Green, T J]]
	[[Category: Luo, M]]		[[Category: Luo, M]]

OCA at 14:15, 25 December 2014

2014-12-25T14:15:41Z

←Older revision		Revision as of 14:15, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 Vesicular stomatitis Indiana virus])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 Vesicular stomatitis Indiana virus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [http://www.ebi.ac.uk/pdbsum/2qvj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [http://www.ebi.ac.uk/pdbsum/2qvj PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NCAP_VSIVA NCAP_VSIVA]] Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Vesicular stomatitis indiana virus]]		[[Category: Vesicular stomatitis indiana virus]]
-	[[Category: Green, T J.]]	+	[[Category: Green, T J]]
-	[[Category: Luo, M.]]	+	[[Category: Luo, M]]
-	[[Category: Qiu, S.]]	+	[[Category: Qiu, S]]
-	[[Category: Tsao, J.]]	+	[[Category: Tsao, J]]
-	[[Category: Zhang, X.]]	+	[[Category: Zhang, X]]
	[[Category: Nucleocapsid]]		[[Category: Nucleocapsid]]
	[[Category: Ribonucleoprotein]]		[[Category: Ribonucleoprotein]]

OCA at 00:58, 2 October 2014

2014-10-02T00:58:58Z

←Older revision		Revision as of 00:58, 2 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2qvj.~~png~~\|~~left\|200px~~]]	+	==Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant==
		+	<StructureSection load='2qvj' size='340' side='right' caption='[[2qvj]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 Vesicular stomatitis Indiana virus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [http://www.ebi.ac.uk/pdbsum/2qvj PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of the vesicular stomatitis virus nucleoprotein (N) in complex with RNA reveals extensive and specific intermolecular interactions among the N molecules in the 10-member oligomer. What roles these interactions play in encapsidating RNA was studied by mutagenesis of the N protein. Three N mutants intended for disruption of the intermolecular interactions were designed and coexpressed with the phosphoprotein (P) in an Escherichia coli system previously described (T. J. Green et al., J. Virol. 74:9515-9524, 2000). Mutants N (Delta1-22), N (Delta347-352), and N (320-324, (Ala)(5)) lost RNA encapsidation and oligomerization but still bound with P. Another mutant, N (Ser290-->Trp), was able to form a stable ring-like N oligomer and bind with the P protein but was no longer able to encapsidate RNA. The crystal structure of N (Ser290-->Trp) at 2.8 A resolution showed that this mutant can maintain all the same intermolecular interactions as the wild-type N except for a slight unwinding of the N-terminal lobe. These results suggest that the intermolecular contacts among the N molecules are required for encapsidation of the viral RNA.

-	~~{{STRUCTURE_2qvj\| PDB=2qvj \| SCENE= }}~~	+	Role of intermolecular interactions of vesicular stomatitis virus nucleoprotein in RNA encapsidation.,Zhang X, Green TJ, Tsao J, Qiu S, Luo M J Virol. 2008 Jan;82(2):674-82. Epub 2007 Nov 14. PMID:18003727<ref>PMID:18003727</ref>

-	~~===Crystal structure~~ of ~~a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18003727}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2qvj]] is a 5 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA].~~	+

	==See Also==		==See Also==
	*[[Nucleoprotein\|Nucleoprotein]]		*[[Nucleoprotein\|Nucleoprotein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:018003727<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Vesicular stomatitis indiana virus]]		[[Category: Vesicular stomatitis indiana virus]]
	[[Category: Green, T J.]]		[[Category: Green, T J.]]

OCA at 17:42, 20 October 2012

2012-10-20T17:42:39Z

←Older revision		Revision as of 17:42, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2qvj]] is a 5 chain structure ~~of [[Nucleoprotein]]~~ with sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA].	+	[[2qvj]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA].

	==See Also==		==See Also==

OCA: Protected "2qvj" [edit=sysop:move=sysop]

2012-07-27T01:46:14Z

Protected "2qvj" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:46, 27 July 2012

OCA at 01:46, 27 July 2012

2012-07-27T01:46:13Z

←Older revision		Revision as of 01:46, 27 July 2012
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-	{{Seed}}
	[[Image:2qvj.png\|left\|200px]]		[[Image:2qvj.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2qvj", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2qvj\| PDB=2qvj \| SCENE= }}		{{STRUCTURE_2qvj\| PDB=2qvj \| SCENE= }}

	===Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant===		===Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18003727}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18003727 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18003727}}		{{ABSTRACT_PUBMED_18003727}}

	==About this Structure==		==About this Structure==
-	~~2QVJ is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA].	+	[[2qvj]] is a 5 chain structure of [[Nucleoprotein]] with sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA].
		+
		+	==See Also==
		+	*[[Nucleoprotein\|Nucleoprotein]]

	==Reference==		==Reference==
-	~~Role of intermolecular interactions of vesicular stomatitis virus nucleoprotein in RNA encapsidation., Zhang X, Green TJ, Tsao J, Qiu S, Luo M, J Virol. 2008 Jan;82(2):674-82. Epub 2007 Nov 14.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18003727 18003727]~~	+	<ref group="xtra">PMID:018003727</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Vesicular stomatitis indiana virus]]		[[Category: Vesicular stomatitis indiana virus]]
	[[Category: Green, T J.]]		[[Category: Green, T J.]]
Line 31:		Line 21:
	[[Category: Tsao, J.]]		[[Category: Tsao, J.]]
	[[Category: Zhang, X.]]		[[Category: Zhang, X.]]
-	[[Category: Cytoplasm]]
	[[Category: Nucleocapsid]]		[[Category: Nucleocapsid]]
	[[Category: Ribonucleoprotein]]		[[Category: Ribonucleoprotein]]
Line 38:		Line 27:
	[[Category: Viral nucleoprotein]]		[[Category: Viral nucleoprotein]]
	[[Category: Virion]]		[[Category: Virion]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:24:08 2008''