2rh2 - Revision history

OCA at 11:57, 30 August 2023

2023-08-30T11:57:08Z

←Older revision		Revision as of 11:57, 30 August 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rh2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RH2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rh2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RH2 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MRD:(4R)~~-~~2-METHYLPENTANE-2~~,~~4-DIOL'>MRD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 0.96Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2gqv\|2gqv]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase]~~, ~~with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rh2 OCA], [https://pdbe.org/2rh2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rh2 RCSB], [https://www.ebi.ac.uk/pdbsum/2rh2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rh2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rh2 OCA], [https://pdbe.org/2rh2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rh2 RCSB], [https://www.ebi.ac.uk/pdbsum/2rh2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rh2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DYR21_ECOLX DYR21_ECOLX]] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.	+	[https://www.uniprot.org/uniprot/DYR21_ECOLX DYR21_ECOLX] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Dihydrofolate reductase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Krahn~~, J M~~]]	+	[[Category: Krahn JM]]
-	[[Category: London~~, R E]]~~	+	[[Category: London RE]]
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Folate metabolism]]~~	+
-	~~[[Category: Methotrexate resistance]]~~	+
-	~~[[Category: Nadp]]~~	+
-	~~[[Category: One-carbon metabolism]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Plasmid-encoded r67 dhfr]]~~	+
-	~~[[Category: Tmp-resistant dhfr]]~~	+
-	~~[[Category: Trimethoprim resistance~~]]	+

OCA at 07:47, 16 March 2022

2022-03-16T07:47:46Z

←Older revision		Revision as of 07:47, 16 March 2022
Line 3:		Line 3:
	<StructureSection load='2rh2' size='340' side='right'caption='[[2rh2]], [[Resolution\|resolution]] 0.96Å' scene=''>		<StructureSection load='2rh2' size='340' side='right'caption='[[2rh2]], [[Resolution\|resolution]] 0.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rh2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2RH2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rh2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RH2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gqv\|2gqv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2gqv\|2gqv]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2rh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rh2 OCA], [~~http~~://pdbe.org/2rh2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2rh2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2rh2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2rh2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rh2 OCA], [https://pdbe.org/2rh2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rh2 RCSB], [https://www.ebi.ac.uk/pdbsum/2rh2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rh2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DYR21_ECOLX DYR21_ECOLX]] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.	+	[[https://www.uniprot.org/uniprot/DYR21_ECOLX DYR21_ECOLX]] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:12, 10 June 2020

2020-06-10T07:12:11Z

←Older revision		Revision as of 07:12, 10 June 2020
Line 1:		Line 1:

	==High Resolution DHFR R-67==		==High Resolution DHFR R-67==
-	<StructureSection load='2rh2' size='340' side='right' caption='[[2rh2]], [[Resolution\|resolution]] 0.96Å' scene=''>	+	<StructureSection load='2rh2' size='340' side='right'caption='[[2rh2]], [[Resolution\|resolution]] 0.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rh2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2RH2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rh2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2RH2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gqv\|2gqv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gqv\|2gqv]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2rh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rh2 OCA], [http://pdbe.org/2rh2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rh2 RCSB], [http://www.ebi.ac.uk/pdbsum/2rh2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rh2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2rh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rh2 OCA], [http://pdbe.org/2rh2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rh2 RCSB], [http://www.ebi.ac.uk/pdbsum/2rh2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rh2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Dihydrofolate reductase\|Dihydrofolate reductase]]	+	*[[Dihydrofolate reductase 3D structures\|Dihydrofolate reductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 29:		Line 29:
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
	[[Category: Krahn, J M]]		[[Category: Krahn, J M]]
	[[Category: London, R E]]		[[Category: London, R E]]

OCA at 18:34, 5 August 2016

2016-08-05T18:34:54Z

←Older revision		Revision as of 18:34, 5 August 2016
Line 1:		Line 1:
		+
	==High Resolution DHFR R-67==		==High Resolution DHFR R-67==
	<StructureSection load='2rh2' size='340' side='right' caption='[[2rh2]], [[Resolution\|resolution]] 0.96Å' scene=''>		<StructureSection load='2rh2' size='340' side='right' caption='[[2rh2]], [[Resolution\|resolution]] 0.96Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gqv\|2gqv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gqv\|2gqv]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rh2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rh2 RCSB], [http://www.ebi.ac.uk/pdbsum/2rh2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rh2 OCA], [http://pdbe.org/2rh2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rh2 RCSB], [http://www.ebi.ac.uk/pdbsum/2rh2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rh2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2rh2" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 21:00, 25 December 2014

2014-12-25T21:00:30Z

←Older revision		Revision as of 21:00, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rh2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RH2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rh2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RH2 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gqv\|2gqv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gqv\|2gqv]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rh2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rh2 RCSB], [http://www.ebi.ac.uk/pdbsum/2rh2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rh2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rh2 RCSB], [http://www.ebi.ac.uk/pdbsum/2rh2 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DYR21_ECOLX DYR21_ECOLX]] Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 27:
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Krahn, J M.]]	+	[[Category: Krahn, J M]]
-	[[Category: London, R E.]]	+	[[Category: London, R E]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Folate metabolism]]		[[Category: Folate metabolism]]

OCA at 01:00, 2 October 2014

2014-10-02T01:00:27Z

←Older revision		Revision as of 01:00, 2 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2rh2.~~png~~\|~~left~~\|~~200px~~]]	+	==High Resolution DHFR R-67==
		+	<StructureSection load='2rh2' size='340' side='right' caption='[[2rh2]], [[Resolution\|resolution]] 0.96Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2rh2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RH2 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gqv\|2gqv]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rh2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rh2 RCSB], [http://www.ebi.ac.uk/pdbsum/2rh2 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type II dihydrofolate reductase (DHFR) is a plasmid-encoded enzyme that confers resistance to bacterial DHFR-targeted antifolate drugs. It forms a symmetric homotetramer with a central pore which functions as the active site. Its unusual structure, which results in a promiscuous binding surface that accommodates either the dihydrofolate (DHF) substrate or the NADPH cofactor, has constituted a significant limitation to efforts to understand its substrate specificity and reaction mechanism. We describe here the first structure of a ternary R67 DHFR.DHF.NADP+ catalytic complex, resolved to 1.26 A. This structure provides the first clear picture of how this enzyme, which lacks the active site carboxyl residue that is ubiquitous in Type I DHFRs, is able to function. In the catalytic complex, the polar backbone atoms of two symmetry-related I68 residues provide recognition motifs that interact with the carboxamide on the nicotinamide ring, and the N3-O4 amide function on the pteridine ring. This set of interactions orients the aromatic rings of substrate and cofactor in a relative endo geometry in which the reactive centers are held in close proximity. Additionally, a central, hydrogen-bonded network consisting of two pairs of Y69-Q67-Q67'-Y69' residues provides an unusually tight interface, which appears to serve as a "molecular clamp" holding the substrates in place in an orientation conducive to hydride transfer. In addition to providing the first clear insight regarding how this extremely unusual enzyme is able to function, the structure of the ternary complex provides general insights into how a mutationally challenged enzyme, i.e., an enzyme whose evolution is restricted to four-residues-at-a-time active site mutations, overcomes this fundamental limitation.

-	~~{{STRUCTURE_2rh2\| PDB=2rh2 \| SCENE= }}~~	+	Crystal structure of a type II dihydrofolate reductase catalytic ternary complex.,Krahn JM, Jackson MR, DeRose EF, Howell EE, London RE Biochemistry. 2007 Dec 25;46(51):14878-88. Epub 2007 Dec 4. PMID:18052202<ref>PMID:18052202</ref>

-	~~===High Resolution DHFR R-67===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18052202}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2rh2]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA].~~	+

	==See Also==		==See Also==
	*[[Dihydrofolate reductase\|Dihydrofolate reductase]]		*[[Dihydrofolate reductase\|Dihydrofolate reductase]]
-	*[[Interferon\|Interferon]]	+	== References ==
-		+	<references/>
-	==~~Reference~~==	+	__TOC__
-	<~~ref group="xtra">PMID:018052202<~~/~~ref~~><~~references group="xtra"~~/>	+	</StructureSection>
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA at 21:35, 20 October 2012

2012-10-20T21:35:23Z

←Older revision		Revision as of 21:35, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2rh2]] is a 1 chain structure ~~of [[Dihydrofolate reductase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA].	+	[[2rh2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA].

	==See Also==		==See Also==
	*[[Dihydrofolate reductase\|Dihydrofolate reductase]]		*[[Dihydrofolate reductase\|Dihydrofolate reductase]]
		+	*[[Interferon\|Interferon]]

	==Reference==		==Reference==

OCA at 13:41, 27 July 2012

2012-07-27T13:41:21Z

←Older revision		Revision as of 13:41, 27 July 2012
Line 1:		Line 1:
	[[Image:2rh2.png\|left\|200px]]		[[Image:2rh2.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2rh2\| PDB=2rh2 \| SCENE= }}		{{STRUCTURE_2rh2\| PDB=2rh2 \| SCENE= }}

	===High Resolution DHFR R-67===		===High Resolution DHFR R-67===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18052202}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18052202 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_18052202}}		{{ABSTRACT_PUBMED_18052202}}

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	==See Also==		==See Also==
-	*[[Dihydrofolate reductase]]	+	*[[Dihydrofolate reductase\|Dihydrofolate reductase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18052202~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018052202</ref><references group="xtra"/>
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA: Protected "2rh2" [edit=sysop:move=sysop]

2010-12-26T23:54:47Z

Protected "2rh2" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:54, 26 December 2010

OCA at 23:54, 26 December 2010

2010-12-26T23:54:46Z

←Older revision		Revision as of 23:54, 26 December 2010
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2rh2.png\|left\|200px]]		[[Image:2rh2.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~2RH2 is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA].	+	[[2rh2]] is a 1 chain structure of [[Dihydrofolate reductase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RH2 OCA].
		+
		+	==See Also==
		+	*[[Dihydrofolate reductase]]

	==Reference==		==Reference==
-	~~Crystal structure of a type II dihydrofolate reductase catalytic ternary complex., Krahn JM, Jackson MR, DeRose EF, Howell EE, London RE, Biochemistry. 2007 Dec 25;46(51):14878-88. Epub 2007 Dec 4.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18052202 18052202]~~	+	<ref group="xtra">PMID:18052202</ref><references group="xtra"/>
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Krahn, J M.]]		[[Category: Krahn, J M.]]
	[[Category: London, R E.]]		[[Category: London, R E.]]
Line 38:		Line 39:
	[[Category: Tmp-resistant dhfr]]		[[Category: Tmp-resistant dhfr]]
	[[Category: Trimethoprim resistance]]		[[Category: Trimethoprim resistance]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:52:43 2008''