2rrd - Revision history

OCA at 07:06, 1 May 2024

2024-05-01T07:06:18Z

←Older revision		Revision as of 07:06, 1 May 2024
Line 1:		Line 1:

	==Structure of HRDC domain from human Bloom syndrome protein, BLM==		==Structure of HRDC domain from human Bloom syndrome protein, BLM==
-	<StructureSection load='2rrd' size='340' side='right'caption='[[2rrd~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2rrd' size='340' side='right'caption='[[2rrd]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rrd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RRD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rrd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RRD FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>BLM, RECQ2, RECQL3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rrd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rrd OCA], [https://pdbe.org/2rrd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rrd RCSB], [https://www.ebi.ac.uk/pdbsum/2rrd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rrd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rrd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rrd OCA], [https://pdbe.org/2rrd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rrd RCSB], [https://www.ebi.ac.uk/pdbsum/2rrd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rrd ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Bloom syndrome. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN] Bloom syndrome. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>	+	[https://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 21:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rrd ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rrd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Bloom syndrome is a rare genetic disorder characterized by severe growth retardation and cancer predisposition. The disease is caused by a loss of function of the Bloom syndrome protein (BLM), a member of the RecQ family of DNA helicases. Here we report on the first 3D structure of a BLM fragment, a solution structure of the C-terminal helicase-and-ribonuclease D-C-terminal (HRDC) domain from human BLM. The structure reveals unique features of BLM HRDC that are distinct from the HRDC domain of Werner syndrome protein. In particular, BLM HRDC retains many acidic residues exposed to the solvent, which makes the domain surface extensively electronegative. Consistent with this, fluorescence polarization assays showed an inability of isolated BLM HRDC to interact with DNA substrates. Analyses employing ultracentrifugation, gel-filtration, CD spectroscopy and dynamic light scattering showed that the BLM HRDC domain exists as a stable monomer in solution. The results show that BLM HRDC is a compact, robust and acidic motif which may play a distinct role apart from DNA binding.
-
-	Solution structure of the HRDC domain of human Bloom syndrome protein BLM.,Sato A, Mishima M, Nagai A, Kim SY, Ito Y, Hakoshima T, Jee JG, Kitano K J Biochem. 2010 Oct;148(4):517-25. Epub 2010 Aug 25. PMID:20739603<ref>PMID:20739603</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2rrd" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hakoshima, T]]	+	[[Category: Hakoshima T]]
-	[[Category: Ito, Y]]	+	[[Category: Ito Y]]
-	[[Category: Jee~~, J G~~]]	+	[[Category: Jee JG]]
-	[[Category: Kim~~, S Y~~]]	+	[[Category: Kim SY]]
-	[[Category: Kitano, K]]	+	[[Category: Kitano K]]
-	[[Category: Mishima, M]]	+	[[Category: Mishima M]]
-	[[Category: Nagai, A]]	+	[[Category: Nagai A]]
-	[[Category: Sato, A]]	+	[[Category: Sato A]]
-	~~[[Category: Bloom syndrome protein]]~~	+
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Dna helicase]]~~	+
-	~~[[Category: Hrdc domain]]~~	+
-	~~[[Category: Recq family~~]]	+

OCA at 10:35, 7 July 2021

2021-07-07T10:35:52Z

←Older revision		Revision as of 10:35, 7 July 2021
Line 1:		Line 1:

	==Structure of HRDC domain from human Bloom syndrome protein, BLM==		==Structure of HRDC domain from human Bloom syndrome protein, BLM==
-	<StructureSection load='2rrd' size='340' side='right' caption='[[2rrd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2rrd' size='340' side='right'caption='[[2rrd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rrd]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2RRD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rrd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RRD FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BLM, RECQ2, RECQL3 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BLM, RECQ2, RECQL3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2rrd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rrd OCA], [~~http~~://pdbe.org/2rrd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2rrd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2rrd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2rrd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rrd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rrd OCA], [https://pdbe.org/2rrd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rrd RCSB], [https://www.ebi.ac.uk/pdbsum/2rrd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rrd ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Bloom syndrome. The disease is caused by mutations affecting the gene represented in this entry.	+	[[https://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Bloom syndrome. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>	+	[[https://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Hakoshima, T]]		[[Category: Hakoshima, T]]
	[[Category: Ito, Y]]		[[Category: Ito, Y]]

OCA at 08:57, 12 September 2018

2018-09-12T08:57:54Z

←Older revision		Revision as of 08:57, 12 September 2018
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rr/2rrd_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rr/2rrd_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 18:30, 11 August 2016

2016-08-11T18:30:22Z

←Older revision		Revision as of 18:30, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of HRDC domain from human Bloom syndrome protein, BLM==		==Structure of HRDC domain from human Bloom syndrome protein, BLM==
	<StructureSection load='2rrd' size='340' side='right' caption='[[2rrd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2rrd' size='340' side='right' caption='[[2rrd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rrd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RRD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rrd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RRD FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BLM, RECQ2, RECQL3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BLM, RECQ2, RECQL3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rrd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rrd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rrd RCSB], [http://www.ebi.ac.uk/pdbsum/2rrd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rrd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rrd OCA], [http://pdbe.org/2rrd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rrd RCSB], [http://www.ebi.ac.uk/pdbsum/2rrd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rrd ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rrd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2rrd" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Hakoshima, T]]		[[Category: Hakoshima, T]]
	[[Category: Ito, Y]]		[[Category: Ito, Y]]

OCA at 06:46, 22 January 2015

2015-01-22T06:46:48Z

←Older revision		Revision as of 06:46, 22 January 2015
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Hakoshima, T.]]	+	[[Category: Hakoshima, T]]
-	[[Category: Ito, Y.]]	+	[[Category: Ito, Y]]
-	[[Category: Jee, J G.]]	+	[[Category: Jee, J G]]
-	[[Category: Kim, S Y.]]	+	[[Category: Kim, S Y]]
-	[[Category: Kitano, K.]]	+	[[Category: Kitano, K]]
-	[[Category: Mishima, M.]]	+	[[Category: Mishima, M]]
-	[[Category: Nagai, A.]]	+	[[Category: Nagai, A]]
-	[[Category: Sato, A.]]	+	[[Category: Sato, A]]
	[[Category: Bloom syndrome protein]]		[[Category: Bloom syndrome protein]]
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]

OCA at 11:32, 20 October 2014

2014-10-20T11:32:02Z

←Older revision		Revision as of 11:32, 20 October 2014
Line 1:		Line 1:
-	[[~~Image~~:2rrd.~~png\|left~~\|200px]]	+	==Structure of HRDC domain from human Bloom syndrome protein, BLM==
		+	<StructureSection load='2rrd' size='340' side='right' caption='[[2rrd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2rrd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RRD FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BLM, RECQ2, RECQL3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rrd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rrd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rrd RCSB], [http://www.ebi.ac.uk/pdbsum/2rrd PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Bloom syndrome. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BLM_HUMAN BLM_HUMAN]] Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).<ref>PMID:9388193</ref> <ref>PMID:12019152</ref> <ref>PMID:21325134</ref> <ref>PMID:23509288</ref>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rr/2rrd_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bloom syndrome is a rare genetic disorder characterized by severe growth retardation and cancer predisposition. The disease is caused by a loss of function of the Bloom syndrome protein (BLM), a member of the RecQ family of DNA helicases. Here we report on the first 3D structure of a BLM fragment, a solution structure of the C-terminal helicase-and-ribonuclease D-C-terminal (HRDC) domain from human BLM. The structure reveals unique features of BLM HRDC that are distinct from the HRDC domain of Werner syndrome protein. In particular, BLM HRDC retains many acidic residues exposed to the solvent, which makes the domain surface extensively electronegative. Consistent with this, fluorescence polarization assays showed an inability of isolated BLM HRDC to interact with DNA substrates. Analyses employing ultracentrifugation, gel-filtration, CD spectroscopy and dynamic light scattering showed that the BLM HRDC domain exists as a stable monomer in solution. The results show that BLM HRDC is a compact, robust and acidic motif which may play a distinct role apart from DNA binding.

-	~~{{STRUCTURE_2rrd\| PDB=2rrd \| SCENE= }}~~	+	Solution structure of the HRDC domain of human Bloom syndrome protein BLM.,Sato A, Mishima M, Nagai A, Kim SY, Ito Y, Hakoshima T, Jee JG, Kitano K J Biochem. 2010 Oct;148(4):517-25. Epub 2010 Aug 25. PMID:20739603<ref>PMID:20739603</ref>

-	~~===Structure of HRDC domain from human Bloom syndrome protein~~, ~~BLM===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20739603}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2rrd]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2RRD OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:020739603<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Hakoshima, T.]]		[[Category: Hakoshima, T.]]

OCA: Protected "2rrd" [edit=sysop:move=sysop]

2013-01-07T21:08:26Z

Protected "2rrd" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:08, 7 January 2013

OCA at 21:08, 7 January 2013

2013-01-07T21:08:22Z

←Older revision		Revision as of 21:08, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2rrd.png\|left\|200px]]		[[Image:2rrd.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2rrd", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2rrd\| PDB=2rrd \| SCENE= }}		{{STRUCTURE_2rrd\| PDB=2rrd \| SCENE= }}

	===Structure of HRDC domain from human Bloom syndrome protein, BLM===		===Structure of HRDC domain from human Bloom syndrome protein, BLM===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20739603}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20739603 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20739603}}		{{ABSTRACT_PUBMED_20739603}}

	==About this Structure==		==About this Structure==
-	~~2RRD~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRD OCA].	+	[[2rrd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRD OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20739603~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020739603</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Hakoshima, T.]]		[[Category: Hakoshima, T.]]
Line 38:		Line 26:
	[[Category: Hrdc domain]]		[[Category: Hrdc domain]]
	[[Category: Recq family]]		[[Category: Recq family]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 20 06:07:18 2010''

OCA at 02:57, 20 October 2010

2010-10-20T02:57:13Z

←Older revision		Revision as of 02:57, 20 October 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:2rrd.~~jpg~~\|left\|200px]]	+	[[Image:2rrd.png\|left\|200px]]

	<!--		<!--
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	===Structure of HRDC domain from human Bloom syndrome protein, BLM===		===Structure of HRDC domain from human Bloom syndrome protein, BLM===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20739603}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20739603 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20739603}}

	==About this Structure==		==About this Structure==
	2RRD is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRD OCA].		2RRD is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRD OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20739603</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Hakoshima, T.]]		[[Category: Hakoshima, T.]]
	[[Category: Ito, Y.]]		[[Category: Ito, Y.]]
-	[[Category: Jee, J.]]	+	[[Category: Jee, J G.]]
-	[[Category: Kim, S.]]	+	[[Category: Kim, S Y.]]
	[[Category: Kitano, K.]]		[[Category: Kitano, K.]]
	[[Category: Mishima, M.]]		[[Category: Mishima, M.]]
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	[[Category: Recq family]]		[[Category: Recq family]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri Sep 10 13~~:53:00 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 20 06:07:18 2010''

OCA at 09:44, 10 September 2010

2010-09-10T09:44:31Z

←Older revision		Revision as of 09:44, 10 September 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:2rrd.jpg\|left\|200px]]

-	The ~~entry 2rrd~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2rrd", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2rrd\| PDB=2rrd \| SCENE= }}

-	~~Authors: Sato~~, ~~A., Mishima, M., Nagai, A., Kim, S., Ito, Y., Hakoshima, T., Jee, J., Kitano, K.~~	+	===Structure of HRDC domain from human Bloom syndrome protein, BLM===

-	Description:

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Aug 18 11~~:15:32 2010''	+	==About this Structure==
		+	2RRD is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRD OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Hakoshima, T.]]
		+	[[Category: Ito, Y.]]
		+	[[Category: Jee, J.]]
		+	[[Category: Kim, S.]]
		+	[[Category: Kitano, K.]]
		+	[[Category: Mishima, M.]]
		+	[[Category: Nagai, A.]]
		+	[[Category: Sato, A.]]
		+	[[Category: Bloom syndrome protein]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Dna helicase]]
		+	[[Category: Hrdc domain]]
		+	[[Category: Recq family]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Sep 10 13:53:00 2010''