2uvo - Revision history

OCA at 14:55, 13 December 2023

2023-12-13T14:55:32Z

←Older revision		Revision as of 14:55, 13 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2uvo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UVO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2uvo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UVO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene~~></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wgc\|1wgc]], [[2cwg\|2cwg]], [[7wga\|7wga]]</div~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvo OCA], [https://pdbe.org/2uvo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uvo RCSB], [https://www.ebi.ac.uk/pdbsum/2uvo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uvo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvo OCA], [https://pdbe.org/2uvo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uvo RCSB], [https://www.ebi.ac.uk/pdbsum/2uvo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uvo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/AGI1_WHEAT AGI1_WHEAT]] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.	+	[https://www.uniprot.org/uniprot/AGI1_WHEAT AGI1_WHEAT] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Triticum aestivum]]		[[Category: Triticum aestivum]]
-	[[Category: Diederichs, K]]	+	[[Category: Diederichs K]]
-	[[Category: Schwefel, D]]	+	[[Category: Schwefel D]]
-	[[Category: Welte, W]]	+	[[Category: Welte W]]
-	[[Category: Wittmann, V]]	+	[[Category: Wittmann V]]
-	~~[[Category: Antifungal]]~~	+
-	~~[[Category: Carbohydrate-binding protein]]~~	+
-	~~[[Category: Chitin]]~~	+
-	~~[[Category: Chitin-binding]]~~	+
-	~~[[Category: Chitin-binding protein]]~~	+
-	~~[[Category: Glcnac]]~~	+
-	~~[[Category: Hevein domain]]~~	+
-	~~[[Category: Lectin]]~~	+
-	~~[[Category: N-acetyl-d- glucosamine]]~~	+
-	~~[[Category: Protein-carbohydrate interaction]]~~	+
-	~~[[Category: Wga]]~~	+
-	~~[[Category: Wheat germ agglutinin~~]]	+

OCA at 17:27, 15 December 2021

2021-12-15T17:27:52Z

←Older revision		Revision as of 17:27, 15 December 2021
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	<StructureSection load='2uvo' size='340' side='right'caption='[[2uvo]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2uvo' size='340' side='right'caption='[[2uvo]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2uvo]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2UVO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2uvo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UVO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wgc\|1wgc]], [[2cwg\|2cwg]], [[7wga\|7wga]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wgc\|1wgc]], [[2cwg\|2cwg]], [[7wga\|7wga]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvo OCA], [~~http~~://pdbe.org/2uvo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2uvo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2uvo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2uvo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvo OCA], [https://pdbe.org/2uvo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uvo RCSB], [https://www.ebi.ac.uk/pdbsum/2uvo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uvo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/AGI1_WHEAT AGI1_WHEAT]] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.	+	[[https://www.uniprot.org/uniprot/AGI1_WHEAT AGI1_WHEAT]] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:39, 11 March 2020

2020-03-11T07:39:31Z

←Older revision		Revision as of 07:39, 11 March 2020
Line 1:		Line 1:

	==High Resolution Crystal Structure of Wheat Germ Agglutinin in Complex with N-Acetyl-D-Glucosamine==		==High Resolution Crystal Structure of Wheat Germ Agglutinin in Complex with N-Acetyl-D-Glucosamine==
-	<StructureSection load='2uvo' size='340' side='right' caption='[[2uvo]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='2uvo' size='340' side='right'caption='[[2uvo]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2uvo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2uvo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVO FirstGlance]. <br>
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Agglutinin\|Agglutinin]]	+	*[[Agglutinin 3D structures\|Agglutinin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Triticum aestivum]]		[[Category: Triticum aestivum]]
	[[Category: Diederichs, K]]		[[Category: Diederichs, K]]

OCA at 08:18, 12 September 2018

2018-09-12T08:18:44Z

←Older revision		Revision as of 08:18, 12 September 2018
Line 1:		Line 1:
-	==~~HIGH RESOLUTION CRYSTAL STRUCTURE OF WHEAT GERM AGGLUTININ IN COMPLEX WITH~~ N-~~ACETYL~~-D-~~GLUCOSAMINE~~==	+
		+	==High Resolution Crystal Structure of Wheat Germ Agglutinin in Complex with N-Acetyl-D-Glucosamine==
	<StructureSection load='2uvo' size='340' side='right' caption='[[2uvo]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2uvo' size='340' side='right' caption='[[2uvo]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wgc\|1wgc]], [[2cwg\|2cwg]], [[7wga\|7wga]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wgc\|1wgc]], [[2cwg\|2cwg]], [[7wga\|7wga]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvo OCA], [http://pdbe.org/2uvo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uvo RCSB], [http://www.ebi.ac.uk/pdbsum/2uvo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvo OCA], [http://pdbe.org/2uvo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uvo RCSB], [http://www.ebi.ac.uk/pdbsum/2uvo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2uvo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uv/2uvo_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uv/2uvo_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 2uvo" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2uvo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Agglutinin\|Agglutinin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:27, 9 February 2016

2016-02-09T02:27:18Z

←Older revision		Revision as of 02:27, 9 February 2016
Line 6:		Line 6:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wgc\|1wgc]], [[2cwg\|2cwg]], [[7wga\|7wga]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wgc\|1wgc]], [[2cwg\|2cwg]], [[7wga\|7wga]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2uvo RCSB], [http://www.ebi.ac.uk/pdbsum/2uvo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvo OCA], [http://pdbe.org/2uvo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uvo RCSB], [http://www.ebi.ac.uk/pdbsum/2uvo PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2uvo ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2uvo" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 03:46, 25 December 2014

2014-12-25T03:46:29Z

←Older revision		Revision as of 03:46, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2uvo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2uvo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVO FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wgc\|1wgc]], [[2cwg\|2cwg]], [[7wga\|7wga]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wgc\|1wgc]], [[2cwg\|2cwg]], [[7wga\|7wga]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2uvo RCSB], [http://www.ebi.ac.uk/pdbsum/2uvo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2uvo RCSB], [http://www.ebi.ac.uk/pdbsum/2uvo PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AGI1_WHEAT AGI1_WHEAT]] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Triticum aestivum]]		[[Category: Triticum aestivum]]
-	[[Category: Diederichs, K.]]	+	[[Category: Diederichs, K]]
-	[[Category: Schwefel, D.]]	+	[[Category: Schwefel, D]]
-	[[Category: Welte, W.]]	+	[[Category: Welte, W]]
-	[[Category: Wittmann, V.]]	+	[[Category: Wittmann, V]]
	[[Category: Antifungal]]		[[Category: Antifungal]]
	[[Category: Carbohydrate-binding protein]]		[[Category: Carbohydrate-binding protein]]

OCA at 01:18, 1 October 2014

2014-10-01T01:18:59Z

←Older revision		Revision as of 01:18, 1 October 2014
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-	[[~~Image:~~2uvo.~~png~~\|~~left~~\|200px]]	+	==HIGH RESOLUTION CRYSTAL STRUCTURE OF WHEAT GERM AGGLUTININ IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE==
		+	<StructureSection load='2uvo' size='340' side='right' caption='[[2uvo]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2uvo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVO FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wgc\|1wgc]], [[2cwg\|2cwg]], [[7wga\|7wga]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2uvo RCSB], [http://www.ebi.ac.uk/pdbsum/2uvo PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uv/2uvo_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The inhibition of carbohydrate-protein interactions by tailored multivalent ligands is a powerful strategy for the treatment of many human diseases. Crucial for the success of this approach is an understanding of the molecular mechanisms as to how a binding enhancement of a multivalent ligand is achieved. We have synthesized a series of multivalent N-acetylglucosamine (GlcNAc) derivatives and studied their interaction with the plant lectin wheat germ agglutinin (WGA) by an enzyme-linked lectin assay (ELLA) and X-ray crystallography. The solution conformation of one ligand was determined by NMR spectroscopy. Employing a GlcNAc carbamate motif with alpha-configuration and by systematic variation of the spacer length, we were able to identify divalent ligands with unprecedented high WGA binding potency. The best divalent ligand has an IC(50) value of 9.8 muM (ELLA) corresponding to a relative potency of 2350 (1170 on a valency-corrected basis, i.e., per mol sugar contained) compared to free GlcNAc. X-ray crystallography of the complex of WGA and the second best, closely related divalent ligand explains this activity. Four divalent molecules simultaneously bind to WGA with each ligand bridging adjacent binding sites. This shows for the first time that all eight sugar binding sites of the WGA dimer are simultaneously functional. We also report a tetravalent neoglycopeptide with an IC(50) value of 0.9 muM being 25 500 times higher than that of GlcNAc (6400 times per contained sugar) and the X-ray structure analysis of its complex with glutaraldehyde-cross-linked WGA. Comparison of the crystal structure and the solution NMR structure of the neoglycopeptide as well as results from the ELLA suggest that the conformation of the glycopeptide in solution is already preorganized in a way supporting multivalent binding to the protein. Our findings show that bridging adjacent protein binding sites by multivalent ligands is a valid strategy to find high-affinity protein ligands and that even subtle changes of the linker structure can have a significant impact on the binding affinity.

-	~~{{STRUCTURE_2uvo\| PDB=2uvo \| SCENE= }}~~	+	Structural Basis of Multivalent Binding to Wheat Germ Agglutinin.,Schwefel D, Maierhofer C, Beck JG, Seeberger S, Diederichs K, Moller HM, Welte W, Wittmann V J Am Chem Soc. 2010 Jun 7. PMID:20527753<ref>PMID:20527753</ref>

-	~~===HIGH RESOLUTION CRYSTAL STRUCTURE OF WHEAT GERM AGGLUTININ IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20527753}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2uvo]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Triticum_aestivum Triticum aestivum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:020527753<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Triticum aestivum]]		[[Category: Triticum aestivum]]
	[[Category: Diederichs, K.]]		[[Category: Diederichs, K.]]

OCA: Protected "2uvo" [edit=sysop:move=sysop]

2013-01-07T19:19:52Z

Protected "2uvo" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:19, 7 January 2013

OCA at 19:19, 7 January 2013

2013-01-07T19:19:49Z

←Older revision		Revision as of 19:19, 7 January 2013
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-	{{Seed}}
	[[Image:2uvo.png\|left\|200px]]		[[Image:2uvo.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2uvo", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2uvo\| PDB=2uvo \| SCENE= }}		{{STRUCTURE_2uvo\| PDB=2uvo \| SCENE= }}

	===HIGH RESOLUTION CRYSTAL STRUCTURE OF WHEAT GERM AGGLUTININ IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE===		===HIGH RESOLUTION CRYSTAL STRUCTURE OF WHEAT GERM AGGLUTININ IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20527753}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20527753 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20527753}}		{{ABSTRACT_PUBMED_20527753}}

	==About this Structure==		==About this Structure==
-	~~2UVO~~ is a 4 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA].	+	[[2uvo]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20527753~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020527753</ref><references group="xtra"/>
	[[Category: Triticum aestivum]]		[[Category: Triticum aestivum]]
	[[Category: Diederichs, K.]]		[[Category: Diederichs, K.]]
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	[[Category: Wga]]		[[Category: Wga]]
	[[Category: Wheat germ agglutinin]]		[[Category: Wheat germ agglutinin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 30 13:24:50 2010''

OCA at 09:19, 30 June 2010

2010-06-30T09:19:15Z

←Older revision		Revision as of 09:19, 30 June 2010
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	===HIGH RESOLUTION CRYSTAL STRUCTURE OF WHEAT GERM AGGLUTININ IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE===		===HIGH RESOLUTION CRYSTAL STRUCTURE OF WHEAT GERM AGGLUTININ IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20527753}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20527753 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20527753}}

	==About this Structure==		==About this Structure==
-	2UVO is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA].	+	2UVO is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVO OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20527753</ref><references group="xtra"/>
	[[Category: Triticum aestivum]]		[[Category: Triticum aestivum]]
	[[Category: Diederichs, K.]]		[[Category: Diederichs, K.]]
Line 33:		Line 42:
	[[Category: Wheat germ agglutinin]]		[[Category: Wheat germ agglutinin]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Feb 17 05~~:43:~~42 2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 30 13:24:50 2010''