2uvv - Revision history

OCA at 14:55, 13 December 2023

2023-12-13T14:55:41Z

←Older revision		Revision as of 14:55, 13 December 2023
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	<StructureSection load='2uvv' size='340' side='right'caption='[[2uvv]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2uvv' size='340' side='right'caption='[[2uvv]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2uvv]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UVV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2uvv]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UVV FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=DGT:~~2~~-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td>~~<td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1jx4\|1jx4]]~~, [[1jxl\|1jxl]], [[1n48\|1n48]], [[1n56\|1n56]], [[1ryr\|1ryr]], [[1rys\|1rys]], [[1s0m\|1s0m]], [[1s0n\|1s0n]], [[1s0o\|1s0o]], [[1s10\|1s10]], [[1s97\|1s97]], [[1s9f\|1s9f]], [[2ago\|2ago]], [[2agp\|2agp]], [[2agq\|2agq]], [[2asd\|2asd]], [[2asj\|2asj]], [[2asl\|2asl]], [[2atl\|2atl]], [[2au0\|2au0]], [[2bq3\|2bq3]], [[2bqr\|2bqr]], [[2bqu\|2bqu]], [[2br0\|2br0]], [[2c22\|2c22]], [[2c28\|2c28]], [[2c2d\|2c2d]], [[2c2e\|2c2e]], [[2c2r\|2c2r]], [[2j6s\|2j6s]], [[2j6t\|2j6t]], [[2j6u\|2j6u]], [[2jef\|2jef]], [[2jeg\|2jeg]], [[2jei\|2jei]], [[2jej\|2jej]], [[2uvr\|2uvr]], [[2uvu\|2uvu]], [[2uvw\|2uvw]]<~~/div></td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA~~-~~directed_DNA_polymerase DNA~~-~~directed DNA polymerase], with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvv OCA], [https://pdbe.org/2uvv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uvv RCSB], [https://www.ebi.ac.uk/pdbsum/2uvv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uvv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvv OCA], [https://pdbe.org/2uvv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uvv RCSB], [https://www.ebi.ac.uk/pdbsum/2uvv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uvv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DPO4_SACS2 DPO4_SACS2] Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. It is involved in translesional synthesis.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</div>		</div>
	<div class="pdbe-citations 2uvv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2uvv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: DNA-directed DNA polymerase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Egli, M]]~~	+	[[Category: Saccharolobus solfataricus P2]]
-	~~[[Category: Irimia, A]]~~	+	[[Category: Egli M]]
-	~~[[Category: 8-dihydro-8- oxodeoxyguanosine]]~~	+	[[Category: Irimia A]]
-	~~[[Category: Dna damage]]~~	+
-	~~[[Category: Dna repair]]~~	+
-	~~[[Category: Dna replication]]~~	+
-	~~[[Category: Dna-binding]]~~	+
-	~~[[Category: Dna-directed dna polymerase]]~~	+
-	~~[[Category: Magnesium]]~~	+
-	~~[[Category: Metal- binding]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Mutator protein]]~~	+
-	~~[[Category: Nucleotidyltransferase]]~~	+
-	~~[[Category:~~ P2 ~~dna polymerase iv~~]]	+
-	[[Category: ~~Transferase~~]]	+
-	[[Category: ~~Translesion dna polymerase~~]]	+

OCA at 10:38, 7 July 2021

2021-07-07T10:38:50Z

←Older revision		Revision as of 10:38, 7 July 2021
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	<StructureSection load='2uvv' size='340' side='right'caption='[[2uvv]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2uvv' size='340' side='right'caption='[[2uvv]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2uvv]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2UVV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2uvv]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UVV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jx4\|1jx4]], [[1jxl\|1jxl]], [[1n48\|1n48]], [[1n56\|1n56]], [[1ryr\|1ryr]], [[1rys\|1rys]], [[1s0m\|1s0m]], [[1s0n\|1s0n]], [[1s0o\|1s0o]], [[1s10\|1s10]], [[1s97\|1s97]], [[1s9f\|1s9f]], [[2ago\|2ago]], [[2agp\|2agp]], [[2agq\|2agq]], [[2asd\|2asd]], [[2asj\|2asj]], [[2asl\|2asl]], [[2atl\|2atl]], [[2au0\|2au0]], [[2bq3\|2bq3]], [[2bqr\|2bqr]], [[2bqu\|2bqu]], [[2br0\|2br0]], [[2c22\|2c22]], [[2c28\|2c28]], [[2c2d\|2c2d]], [[2c2e\|2c2e]], [[2c2r\|2c2r]], [[2j6s\|2j6s]], [[2j6t\|2j6t]], [[2j6u\|2j6u]], [[2jef\|2jef]], [[2jeg\|2jeg]], [[2jei\|2jei]], [[2jej\|2jej]], [[2uvr\|2uvr]], [[2uvu\|2uvu]], [[2uvw\|2uvw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1jx4\|1jx4]], [[1jxl\|1jxl]], [[1n48\|1n48]], [[1n56\|1n56]], [[1ryr\|1ryr]], [[1rys\|1rys]], [[1s0m\|1s0m]], [[1s0n\|1s0n]], [[1s0o\|1s0o]], [[1s10\|1s10]], [[1s97\|1s97]], [[1s9f\|1s9f]], [[2ago\|2ago]], [[2agp\|2agp]], [[2agq\|2agq]], [[2asd\|2asd]], [[2asj\|2asj]], [[2asl\|2asl]], [[2atl\|2atl]], [[2au0\|2au0]], [[2bq3\|2bq3]], [[2bqr\|2bqr]], [[2bqu\|2bqu]], [[2br0\|2br0]], [[2c22\|2c22]], [[2c28\|2c28]], [[2c2d\|2c2d]], [[2c2e\|2c2e]], [[2c2r\|2c2r]], [[2j6s\|2j6s]], [[2j6t\|2j6t]], [[2j6u\|2j6u]], [[2jef\|2jef]], [[2jeg\|2jeg]], [[2jei\|2jei]], [[2jej\|2jej]], [[2uvr\|2uvr]], [[2uvu\|2uvu]], [[2uvw\|2uvw]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2uvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvv OCA], [~~http~~://pdbe.org/2uvv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2uvv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2uvv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2uvv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvv OCA], [https://pdbe.org/2uvv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uvv RCSB], [https://www.ebi.ac.uk/pdbsum/2uvv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uvv ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</div>		</div>
	<div class="pdbe-citations 2uvv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2uvv" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:18, 21 August 2019

2019-08-21T07:18:25Z

←Older revision		Revision as of 07:18, 21 August 2019
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	==Crystal structures of mutant Dpo4 DNA polymerases with 8-oxoG containing DNA template-primer constructs==		==Crystal structures of mutant Dpo4 DNA polymerases with 8-oxoG containing DNA template-primer constructs==
-	<StructureSection load='2uvv' size='340' side='right' caption='[[2uvv]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='2uvv' size='340' side='right'caption='[[2uvv]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2uvv]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2uvv]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVV FirstGlance]. <br>
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uv/2uvv_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uv/2uvv_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 2uvv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2uvv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
		+	[[Category: Large Structures]]
	[[Category: Egli, M]]		[[Category: Egli, M]]
	[[Category: Irimia, A]]		[[Category: Irimia, A]]

OCA at 10:16, 16 November 2017

2017-11-16T10:16:21Z

←Older revision		Revision as of 10:16, 16 November 2017
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	</div>		</div>
	<div class="pdbe-citations 2uvv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2uvv" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[DNA polymerase\|DNA polymerase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:44, 3 August 2017

2017-08-03T08:44:39Z

←Older revision		Revision as of 08:44, 3 August 2017
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-	==~~CRYSTAL STRUCTURES OF MUTANT DPO4~~ DNA ~~POLYMERASES WITH~~ 8-~~OXOG CONTAINING~~ DNA ~~TEMPLATE~~-~~PRIMER CONSTRUCTS~~==	+
		+	==Crystal structures of mutant Dpo4 DNA polymerases with 8-oxoG containing DNA template-primer constructs==
	<StructureSection load='2uvv' size='340' side='right' caption='[[2uvv]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2uvv' size='340' side='right' caption='[[2uvv]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2uvv]] is a 3 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Sulso Sulso]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2uvv]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jx4\|1jx4]], [[1jxl\|1jxl]], [[1n48\|1n48]], [[1n56\|1n56]], [[1ryr\|1ryr]], [[1rys\|1rys]], [[1s0m\|1s0m]], [[1s0n\|1s0n]], [[1s0o\|1s0o]], [[1s10\|1s10]], [[1s97\|1s97]], [[1s9f\|1s9f]], [[2ago\|2ago]], [[2agp\|2agp]], [[2agq\|2agq]], [[2asd\|2asd]], [[2asj\|2asj]], [[2asl\|2asl]], [[2atl\|2atl]], [[2au0\|2au0]], [[2bq3\|2bq3]], [[2bqr\|2bqr]], [[2bqu\|2bqu]], [[2br0\|2br0]], [[2c22\|2c22]], [[2c28\|2c28]], [[2c2d\|2c2d]], [[2c2e\|2c2e]], [[2c2r\|2c2r]], [[2j6s\|2j6s]], [[2j6t\|2j6t]], [[2j6u\|2j6u]], [[2jef\|2jef]], [[2jeg\|2jeg]], [[2jei\|2jei]], [[2jej\|2jej]], [[2uvr\|2uvr]], [[2uvu\|2uvu]], [[2uvw\|2uvw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jx4\|1jx4]], [[1jxl\|1jxl]], [[1n48\|1n48]], [[1n56\|1n56]], [[1ryr\|1ryr]], [[1rys\|1rys]], [[1s0m\|1s0m]], [[1s0n\|1s0n]], [[1s0o\|1s0o]], [[1s10\|1s10]], [[1s97\|1s97]], [[1s9f\|1s9f]], [[2ago\|2ago]], [[2agp\|2agp]], [[2agq\|2agq]], [[2asd\|2asd]], [[2asj\|2asj]], [[2asl\|2asl]], [[2atl\|2atl]], [[2au0\|2au0]], [[2bq3\|2bq3]], [[2bqr\|2bqr]], [[2bqu\|2bqu]], [[2br0\|2br0]], [[2c22\|2c22]], [[2c28\|2c28]], [[2c2d\|2c2d]], [[2c2e\|2c2e]], [[2c2r\|2c2r]], [[2j6s\|2j6s]], [[2j6t\|2j6t]], [[2j6u\|2j6u]], [[2jef\|2jef]], [[2jeg\|2jeg]], [[2jei\|2jei]], [[2jej\|2jej]], [[2uvr\|2uvr]], [[2uvu\|2uvu]], [[2uvw\|2uvw]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvv OCA], [http://pdbe.org/2uvv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uvv RCSB], [http://www.ebi.ac.uk/pdbsum/2uvv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvv OCA], [http://pdbe.org/2uvv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uvv RCSB], [http://www.ebi.ac.uk/pdbsum/2uvv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2uvv ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 36:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
-	[[Category: Sulso]]
	[[Category: Egli, M]]		[[Category: Egli, M]]
	[[Category: Irimia, A]]		[[Category: Irimia, A]]

OCA at 14:43, 7 February 2016

2016-02-07T14:43:25Z

←Older revision		Revision as of 14:43, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='2uvv' size='340' side='right' caption='[[2uvv]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2uvv' size='340' side='right' caption='[[2uvv]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2uvv]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus Sulfolobus solfataricus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2uvv]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulso Sulso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jx4\|1jx4]], [[1jxl\|1jxl]], [[1n48\|1n48]], [[1n56\|1n56]], [[1ryr\|1ryr]], [[1rys\|1rys]], [[1s0m\|1s0m]], [[1s0n\|1s0n]], [[1s0o\|1s0o]], [[1s10\|1s10]], [[1s97\|1s97]], [[1s9f\|1s9f]], [[2ago\|2ago]], [[2agp\|2agp]], [[2agq\|2agq]], [[2asd\|2asd]], [[2asj\|2asj]], [[2asl\|2asl]], [[2atl\|2atl]], [[2au0\|2au0]], [[2bq3\|2bq3]], [[2bqr\|2bqr]], [[2bqu\|2bqu]], [[2br0\|2br0]], [[2c22\|2c22]], [[2c28\|2c28]], [[2c2d\|2c2d]], [[2c2e\|2c2e]], [[2c2r\|2c2r]], [[2j6s\|2j6s]], [[2j6t\|2j6t]], [[2j6u\|2j6u]], [[2jef\|2jef]], [[2jeg\|2jeg]], [[2jei\|2jei]], [[2jej\|2jej]], [[2uvr\|2uvr]], [[2uvu\|2uvu]], [[2uvw\|2uvw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jx4\|1jx4]], [[1jxl\|1jxl]], [[1n48\|1n48]], [[1n56\|1n56]], [[1ryr\|1ryr]], [[1rys\|1rys]], [[1s0m\|1s0m]], [[1s0n\|1s0n]], [[1s0o\|1s0o]], [[1s10\|1s10]], [[1s97\|1s97]], [[1s9f\|1s9f]], [[2ago\|2ago]], [[2agp\|2agp]], [[2agq\|2agq]], [[2asd\|2asd]], [[2asj\|2asj]], [[2asl\|2asl]], [[2atl\|2atl]], [[2au0\|2au0]], [[2bq3\|2bq3]], [[2bqr\|2bqr]], [[2bqu\|2bqu]], [[2br0\|2br0]], [[2c22\|2c22]], [[2c28\|2c28]], [[2c2d\|2c2d]], [[2c2e\|2c2e]], [[2c2r\|2c2r]], [[2j6s\|2j6s]], [[2j6t\|2j6t]], [[2j6u\|2j6u]], [[2jef\|2jef]], [[2jeg\|2jeg]], [[2jei\|2jei]], [[2jej\|2jej]], [[2uvr\|2uvr]], [[2uvu\|2uvu]], [[2uvw\|2uvw]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2uvv RCSB], [http://www.ebi.ac.uk/pdbsum/2uvv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvv OCA], [http://pdbe.org/2uvv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uvv RCSB], [http://www.ebi.ac.uk/pdbsum/2uvv PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2uvv ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2uvv" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
-	[[Category: ~~Sulfolobus solfataricus~~]]	+	[[Category: Sulso]]
	[[Category: Egli, M]]		[[Category: Egli, M]]
	[[Category: Irimia, A]]		[[Category: Irimia, A]]

OCA at 16:34, 19 January 2015

2015-01-19T16:34:50Z

←Older revision		Revision as of 16:34, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2uvv]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2uvv]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVV FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jx4\|1jx4]], [[1jxl\|1jxl]], [[1n48\|1n48]], [[1n56\|1n56]], [[1ryr\|1ryr]], [[1rys\|1rys]], [[1s0m\|1s0m]], [[1s0n\|1s0n]], [[1s0o\|1s0o]], [[1s10\|1s10]], [[1s97\|1s97]], [[1s9f\|1s9f]], [[2ago\|2ago]], [[2agp\|2agp]], [[2agq\|2agq]], [[2asd\|2asd]], [[2asj\|2asj]], [[2asl\|2asl]], [[2atl\|2atl]], [[2au0\|2au0]], [[2bq3\|2bq3]], [[2bqr\|2bqr]], [[2bqu\|2bqu]], [[2br0\|2br0]], [[2c22\|2c22]], [[2c28\|2c28]], [[2c2d\|2c2d]], [[2c2e\|2c2e]], [[2c2r\|2c2r]], [[2j6s\|2j6s]], [[2j6t\|2j6t]], [[2j6u\|2j6u]], [[2jef\|2jef]], [[2jeg\|2jeg]], [[2jei\|2jei]], [[2jej\|2jej]], [[2uvr\|2uvr]], [[2uvu\|2uvu]], [[2uvw\|2uvw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jx4\|1jx4]], [[1jxl\|1jxl]], [[1n48\|1n48]], [[1n56\|1n56]], [[1ryr\|1ryr]], [[1rys\|1rys]], [[1s0m\|1s0m]], [[1s0n\|1s0n]], [[1s0o\|1s0o]], [[1s10\|1s10]], [[1s97\|1s97]], [[1s9f\|1s9f]], [[2ago\|2ago]], [[2agp\|2agp]], [[2agq\|2agq]], [[2asd\|2asd]], [[2asj\|2asj]], [[2asl\|2asl]], [[2atl\|2atl]], [[2au0\|2au0]], [[2bq3\|2bq3]], [[2bqr\|2bqr]], [[2bqu\|2bqu]], [[2br0\|2br0]], [[2c22\|2c22]], [[2c28\|2c28]], [[2c2d\|2c2d]], [[2c2e\|2c2e]], [[2c2r\|2c2r]], [[2j6s\|2j6s]], [[2j6t\|2j6t]], [[2j6u\|2j6u]], [[2jef\|2jef]], [[2jeg\|2jeg]], [[2jei\|2jei]], [[2jej\|2jej]], [[2uvr\|2uvr]], [[2uvu\|2uvu]], [[2uvw\|2uvw]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2uvv RCSB], [http://www.ebi.ac.uk/pdbsum/2uvv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2uvv RCSB], [http://www.ebi.ac.uk/pdbsum/2uvv PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
-	[[Category: Egli, M.]]	+	[[Category: Egli, M]]
-	[[Category: Irimia, A.]]	+	[[Category: Irimia, A]]
	[[Category: 8-dihydro-8- oxodeoxyguanosine]]		[[Category: 8-dihydro-8- oxodeoxyguanosine]]
	[[Category: Dna damage]]		[[Category: Dna damage]]

OCA at 08:37, 29 September 2014

2014-09-29T08:37:36Z

←Older revision		Revision as of 08:37, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2uvv.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURES OF MUTANT DPO4 DNA POLYMERASES WITH 8-OXOG CONTAINING DNA TEMPLATE-PRIMER CONSTRUCTS==
		+	<StructureSection load='2uvv' size='340' side='right' caption='[[2uvv]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2uvv]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UVV FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jx4\|1jx4]], [[1jxl\|1jxl]], [[1n48\|1n48]], [[1n56\|1n56]], [[1ryr\|1ryr]], [[1rys\|1rys]], [[1s0m\|1s0m]], [[1s0n\|1s0n]], [[1s0o\|1s0o]], [[1s10\|1s10]], [[1s97\|1s97]], [[1s9f\|1s9f]], [[2ago\|2ago]], [[2agp\|2agp]], [[2agq\|2agq]], [[2asd\|2asd]], [[2asj\|2asj]], [[2asl\|2asl]], [[2atl\|2atl]], [[2au0\|2au0]], [[2bq3\|2bq3]], [[2bqr\|2bqr]], [[2bqu\|2bqu]], [[2br0\|2br0]], [[2c22\|2c22]], [[2c28\|2c28]], [[2c2d\|2c2d]], [[2c2e\|2c2e]], [[2c2r\|2c2r]], [[2j6s\|2j6s]], [[2j6t\|2j6t]], [[2j6u\|2j6u]], [[2jef\|2jef]], [[2jeg\|2jeg]], [[2jei\|2jei]], [[2jej\|2jej]], [[2uvr\|2uvr]], [[2uvu\|2uvu]], [[2uvw\|2uvw]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uvv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2uvv RCSB], [http://www.ebi.ac.uk/pdbsum/2uvv PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uv/2uvv_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) has been shown to catalyze bypass of 7,8-dihydro-8-oxodeoxyguanosine (8-oxoG) in a highly efficient and relatively accurate manner. Crystal structures have revealed a potential role for Arg(332) in stabilizing the anti conformation of the 8-oxoG template base by means of a hydrogen bond or ion-dipole pair, which results in an increased enzymatic efficiency for dCTP insertion and makes formation of a Hoogsteen pair between 8-oxoG and dATP less favorable. Site-directed mutagenesis was used to replace Arg(332) with Ala, Glu, Leu, or His in order to probe the importance of Arg(332) in accurate and efficient bypass of 8-oxoG. The double mutant Ala(331)Ala(332) was also prepared to address the contribution of Arg(331). Transientstate kinetic results suggest that Glu(332) retains fidelity against bypass of 8-oxoG that is similar to wild type Dpo4, a result that was confirmed by tandem mass spectrometric analysis of full-length extension products. A crystal structure of the Dpo4 Glu(332) mutant and 8-oxoG:C pair revealed water-mediated hydrogen bonds between Glu(332) and the O-8 atom of 8-oxoG. The space normally occupied by Arg(332) side chain is empty in the crystal structures of the Ala(332) mutant. Two other crystal structures show that a Hoogsteen base pair is formed between 8-oxoG and A in the active site of both Glu(332) and Ala(332) mutants. These results support the view that a bond between Arg(332) and 8-oxoG plays a role in determining the fidelity and efficiency of Dpo4-catalyzed bypass of the lesion.

-	~~{{STRUCTURE_2uvv\| PDB=2uvv \| SCENE= }}~~	+	Hydrogen bonding of 7,8-dihydro-8-oxodeoxyguanosine with a charged residue in the little finger domain determines miscoding events in Sulfolobus solfataricus DNA polymerase Dpo4.,Eoff RL, Irimia A, Angel KC, Egli M, Guengerich FP J Biol Chem. 2007 Jul 6;282(27):19831-43. Epub 2007 Apr 27. PMID:17468100<ref>PMID:17468100</ref>

-	~~===CRYSTAL STRUCTURES OF MUTANT DPO4 DNA POLYMERASES WITH 8-OXOG CONTAINING DNA TEMPLATE-PRIMER CONSTRUCTS===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17468100}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2uvv]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA].~~	+

	==See Also==		==See Also==
	*[[DNA polymerase\|DNA polymerase]]		*[[DNA polymerase\|DNA polymerase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:017468100<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]

OCA at 06:57, 20 October 2012

2012-10-20T06:57:35Z

←Older revision		Revision as of 06:57, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[2uvv]] is a 3 chain structure ~~of [[DNA polymerase]]~~ with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA].	+	[[2uvv]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UVV OCA].

	==See Also==		==See Also==

OCA: Protected "2uvv" [edit=sysop:move=sysop]

2012-07-25T14:51:26Z

Protected "2uvv" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:51, 25 July 2012