2vpr - Revision history

OCA at 15:28, 13 December 2023

2023-12-13T15:28:13Z

←Older revision		Revision as of 15:28, 13 December 2023
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	<StructureSection load='2vpr' size='340' side='right'caption='[[2vpr]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='2vpr' size='340' side='right'caption='[[2vpr]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vpr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VPR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vpr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VPR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.49Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpr OCA], [https://pdbe.org/2vpr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vpr RCSB], [https://www.ebi.ac.uk/pdbsum/2vpr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vpr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpr OCA], [https://pdbe.org/2vpr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vpr RCSB], [https://www.ebi.ac.uk/pdbsum/2vpr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vpr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TETR8_PASMD TETR8_PASMD] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacterium multocidum lehmann and neumann 1899]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Hinrichs, W~~]]	+	[[Category: Pasteurella multocida]]
-	[[Category: ~~Palm, G~~]]	+	[[Category: Hinrichs W]]
-	[[Category: ~~Schuldt, L~~]]	+	[[Category: Palm G]]
-	[[Category: ~~Antibiotic resistance]]~~	+	[[Category: Schuldt L]]
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription regulation dna-binding]]~~	+
-	~~[[Category: Transcription regulator~~]]	+

OCA at 10:48, 7 July 2021

2021-07-07T10:48:10Z

←Older revision		Revision as of 10:48, 7 July 2021
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	<StructureSection load='2vpr' size='340' side='right'caption='[[2vpr]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='2vpr' size='340' side='right'caption='[[2vpr]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vpr]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2VPR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vpr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VPR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2vpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpr OCA], [~~http~~://pdbe.org/2vpr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2vpr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2vpr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2vpr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpr OCA], [https://pdbe.org/2vpr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vpr RCSB], [https://www.ebi.ac.uk/pdbsum/2vpr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vpr ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:41, 24 July 2019

2019-07-24T07:41:22Z

←Older revision		Revision as of 07:41, 24 July 2019
Line 1:		Line 1:

	==Tet repressor class H in complex with 5a,6- anhydrotetracycline-Mg==		==Tet repressor class H in complex with 5a,6- anhydrotetracycline-Mg==
-	<StructureSection load='2vpr' size='340' side='right' caption='[[2vpr]], [[Resolution\|resolution]] 2.49Å' scene=''>	+	<StructureSection load='2vpr' size='340' side='right'caption='[[2vpr]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vpr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VPR FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vpr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VPR FirstGlance]. <br>
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Bacterium multocidum lehmann and neumann 1899]]		[[Category: Bacterium multocidum lehmann and neumann 1899]]
		+	[[Category: Large Structures]]
	[[Category: Hinrichs, W]]		[[Category: Hinrichs, W]]
	[[Category: Palm, G]]		[[Category: Palm, G]]

OCA at 20:41, 19 September 2018

2018-09-19T20:41:06Z

←Older revision		Revision as of 20:41, 19 September 2018
Line 1:		Line 1:

-	==~~TET REPRESSOR CLASS~~ H ~~IN COMPLEX WITH 5A~~,6-~~ANHYDROTETRACYCLINE~~-MG==	+	==Tet repressor class H in complex with 5a,6- anhydrotetracycline-Mg==
	<StructureSection load='2vpr' size='340' side='right' caption='[[2vpr]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='2vpr' size='340' side='right' caption='[[2vpr]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 11:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vp/2vpr_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vp/2vpr_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 11:40, 11 August 2016

2016-08-11T11:40:23Z

←Older revision		Revision as of 11:40, 11 August 2016
Line 1:		Line 1:
		+
	==TET REPRESSOR CLASS H IN COMPLEX WITH 5A,6-ANHYDROTETRACYCLINE-MG==		==TET REPRESSOR CLASS H IN COMPLEX WITH 5A,6-ANHYDROTETRACYCLINE-MG==
	<StructureSection load='2vpr' size='340' side='right' caption='[[2vpr]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='2vpr' size='340' side='right' caption='[[2vpr]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vpr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pasteurella_multocida Pasteurella multocida~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VPR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vpr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VPR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vpr RCSB], [http://www.ebi.ac.uk/pdbsum/2vpr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpr OCA], [http://pdbe.org/2vpr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vpr RCSB], [http://www.ebi.ac.uk/pdbsum/2vpr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vpr ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vpr ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2vpr" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pasteurella multocida~~]]	+	[[Category: Bacterium multocidum lehmann and neumann 1899]]
	[[Category: Hinrichs, W]]		[[Category: Hinrichs, W]]
	[[Category: Palm, G]]		[[Category: Palm, G]]

OCA at 15:04, 19 January 2015

2015-01-19T15:04:43Z

←Older revision		Revision as of 15:04, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vpr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VPR FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vpr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VPR FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vpr RCSB], [http://www.ebi.ac.uk/pdbsum/2vpr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vpr RCSB], [http://www.ebi.ac.uk/pdbsum/2vpr PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Pasteurella multocida]]		[[Category: Pasteurella multocida]]
-	[[Category: Hinrichs, W.]]	+	[[Category: Hinrichs, W]]
-	[[Category: Palm, G.]]	+	[[Category: Palm, G]]
-	[[Category: Schuldt, L.]]	+	[[Category: Schuldt, L]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]

OCA at 01:53, 1 October 2014

2014-10-01T01:53:23Z

←Older revision		Revision as of 01:53, 1 October 2014
Line 1:		Line 1:
-	[[~~Image~~:2vpr.~~png\|left~~\|200px]]	+	==TET REPRESSOR CLASS H IN COMPLEX WITH 5A,6-ANHYDROTETRACYCLINE-MG==
		+	<StructureSection load='2vpr' size='340' side='right' caption='[[2vpr]], [[Resolution\|resolution]] 2.49Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2vpr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VPR FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TDC:5A,6-ANHYDROTETRACYCLINE'>TDC</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vpr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vpr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vpr RCSB], [http://www.ebi.ac.uk/pdbsum/2vpr PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vp/2vpr_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Tet repressor (TetR) mediates the most important mechanism of bacterial resistance against tetracycline (Tc) antibiotics. In the absence of Tc, TetR is tightly bound to its operator DNA; upon binding of Tc with an associated Mg(2+) ion, it dissociates from the DNA, allowing expression of the repressed genes. Its tight control by Tc makes TetR broadly useful in genetic engineering. The Tc binding site is over 20 A from the DNA, so the binding signal must propagate a long distance. We use molecular dynamics simulations and continuum electrostatic calculations to test two models of the allosteric mechanism. We simulate the TetR:DNA complex, the Tc-bound, "induced" TetR, and the transition pathway between them. The simulations support the model inferred previously from the crystal structures and reveal new details. When [Tc:Mg](+) binds, the Mg(2+) ion makes direct and water-mediated interactions with helix 8 of one TetR monomer and helix 6 of the other monomer, and helix 6 is pulled in towards the central core of the structure. Hydrophobic interactions with helix 6 then pull helix 4 in a pendulum motion, with a maximal displacement at its N-terminus: the DNA interface. The crystal structure of an additional TetR reported here corroborates this motion. The N-terminal residue of helix 4, Lys48, is highly conserved in DNA-binding regulatory proteins of the TetR class and makes the largest contribution of any amino acid to the TetR:DNA binding free energy. Thus, the conformational changes lead to a drastic reduction in the TetR:DNA binding affinity, allowing TetR to detach itself from the DNA. Tc plays the role of a specific Mg(2+) carrier, whereas the Mg(2+) ion itself makes key interactions that trigger the allosteric transition in the TetR:Tc complex.

-	~~{{STRUCTURE_2vpr\| PDB=2vpr \| SCENE= }}~~	+	Tet repressor induction by tetracycline: a molecular dynamics, continuum electrostatics, and crystallographic study.,Aleksandrov A, Schuldt L, Hinrichs W, Simonson T J Mol Biol. 2008 May 9;378(4):898-912. Epub 2008 Mar 19. PMID:18395746<ref>PMID:18395746</ref>

-	~~===TET REPRESSOR CLASS H IN COMPLEX WITH 5A~~,~~6-ANHYDROTETRACYCLINE-MG===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18395746}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2vpr]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pasteurella_multocida Pasteurella multocida]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018395746<~~/~~ref~~><~~ref group="xtra">PMID:010700280</ref><ref group="xtra">PMID:008153629</ref><references group="xtra"~~/>	+
	[[Category: Pasteurella multocida]]		[[Category: Pasteurella multocida]]
	[[Category: Hinrichs, W.]]		[[Category: Hinrichs, W.]]

OCA at 13:07, 30 January 2013

2013-01-30T13:07:33Z

←Older revision		Revision as of 13:07, 30 January 2013
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	[[Image:2vpr.png\|left\|200px]]		[[Image:2vpr.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2vpr\| PDB=2vpr \| SCENE= }}		{{STRUCTURE_2vpr\| PDB=2vpr \| SCENE= }}

	===TET REPRESSOR CLASS H IN COMPLEX WITH 5A,6-ANHYDROTETRACYCLINE-MG===		===TET REPRESSOR CLASS H IN COMPLEX WITH 5A,6-ANHYDROTETRACYCLINE-MG===

-
-	<!--
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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18395746~~</ref><ref group="xtra">PMID:~~10700280~~</ref><ref group="xtra">PMID:~~8153629~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018395746</ref><ref group="xtra">PMID:010700280</ref><ref group="xtra">PMID:008153629</ref><references group="xtra"/>
	[[Category: Pasteurella multocida]]		[[Category: Pasteurella multocida]]
	[[Category: Hinrichs, W.]]		[[Category: Hinrichs, W.]]
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	[[Category: Schuldt, L.]]		[[Category: Schuldt, L.]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
-	[[Category: Dna-binding]]
-	[[Category: Magnesium]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
-	[[Category: Plasmid]]
-	[[Category: Repressor]]
	[[Category: Transcription]]		[[Category: Transcription]]
-	[[Category: Transcription regulation]]	+	[[Category: Transcription regulation dna-binding]]
	[[Category: Transcription regulator]]		[[Category: Transcription regulator]]

OCA: Protected "2vpr" [edit=sysop:move=sysop]

2011-03-14T19:54:48Z

Protected "2vpr" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:54, 14 March 2011

OCA at 19:54, 14 March 2011

2011-03-14T19:54:46Z

←Older revision		Revision as of 19:54, 14 March 2011
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-	{{Seed}}
	[[Image:2vpr.png\|left\|200px]]		[[Image:2vpr.png\|left\|200px]]

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	===TET REPRESSOR CLASS H IN COMPLEX WITH 5A,6-ANHYDROTETRACYCLINE-MG===		===TET REPRESSOR CLASS H IN COMPLEX WITH 5A,6-ANHYDROTETRACYCLINE-MG===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18395746}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18395746 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18395746}}

	==About this Structure==		==About this Structure==
-	~~2VPR~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA].	+	[[2vpr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:10700280</ref><ref group="xtra">PMID:8153629</ref><references group="xtra"/>	+	<ref group="xtra">PMID:18395746</ref><ref group="xtra">PMID:10700280</ref><ref group="xtra">PMID:8153629</ref><references group="xtra"/>
	[[Category: Pasteurella multocida]]		[[Category: Pasteurella multocida]]
	[[Category: Hinrichs, W.]]		[[Category: Hinrichs, W.]]
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	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
	[[Category: Transcription regulator]]		[[Category: Transcription regulator]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:29:39 2009''